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Replication of Escherichia coli requires DNA polymerase I 总被引:15,自引:0,他引:15
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Isolation of an E. coli strain with a mutation affecting DNA polymerase 总被引:165,自引:0,他引:165
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Effect of antiserum to E. coli DNA polymerase on synthesis of phi-X174 DNA in extracts of phi-X-infected cells 总被引:1,自引:0,他引:1
D T Denhardt 《Nature》1968,220(5163):131-134
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New RNA polymerase from Escherichia coli infected with bacteriophage T7 总被引:89,自引:0,他引:89
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Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase. 总被引:53,自引:0,他引:53
The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the antimetabolic drug trimethoprim. 相似文献
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Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP 总被引:1,自引:0,他引:1
The 3.3-A resolution crystal structure of the large proteolytic fragment of Escherichia coli DNA polymerase I complexed with deoxythymidine monophosphate consists of two domains, the smaller of which binds zinc-deoxythymidine monophosphate. The most striking feature of the larger domain is a deep crevice of the appropriate size and shape for binding double-stranded B-DNA. A flexible subdomain may allow the enzyme to surround completely the DNA substrate, thereby allowing processive nucleotide polymerization without enzyme dissociation. 相似文献
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DNA restriction enzyme from E. coli 总被引:116,自引:0,他引:116
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用聚合酶链式反应技术,从T7噬菌体中选择扩增编码T7DNA滞酶5’→3’聚合酶的基因序列,扩增片段利用引入的酶切位点克隆至载体PSK上,从而得到了不含有3’→5’外切酶基因的T7DNA聚合酶基因,并将该重组基因克隆至原核表达质粒pET28a上,在大肠杆菌中进行了表达。 相似文献
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Mutant of E. coli containing an altered DNA-dependent RNA polymerase 总被引:17,自引:0,他引:17
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Nucleotide sequence of E. coli B tRNA1-Val 总被引:16,自引:0,他引:16
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Single-molecule studies of the effect of template tension on T7 DNA polymerase activity 总被引:8,自引:0,他引:8
T7 DNA polymerase catalyses DNA replication in vitro at rates of more than 100 bases per second and has a 3'-->5' exonuclease (nucleotide removing) activity at a separate active site. This enzyme possesses a 'right hand' shape which is common to most polymerases with fingers, palm and thumb domains. The rate-limiting step for replication is thought to involve a conformational change between an 'open fingers' state in which the active site samples nucleotides, and a 'closed' state in which nucleotide incorporation occurs. DNA polymerase must function as a molecular motor converting chemical energy into mechanical force as it moves over the template. Here we show, using a single-molecule assay based on the differential elasticity of single-stranded and double-stranded DNA, that mechanical force is generated during the rate-limiting step and that the motor can work against a maximum template tension of approximately 34 pN. Estimates of the mechanical and entropic work done by the enzyme show that T7 DNA polymerase organizes two template bases in the polymerization site during each catalytic cycle. We also find a force-induced 100-fold increase in exonucleolysis above 40 pN. 相似文献
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ZHU Liqin LI Hui XIONG Jinhu WANG Tongxiang OU Xuan WEI Yun WU Xinxing~ Institute of Virology Medical School Wuhan University Wuhan Hubei China 《武汉大学学报:自然科学英文版》2006,11(3):749-755
0 IntroductionInbroicguhltat einxpge cDtaNtiAonvafcocri nper eivse ant ninegw aanpdp cruoraicnhg wdiits-heases . Due to the comparative weakness of cellulari mmunity that is purely induced by E7 DNA vac-cine,research on DNA vaccine is now focused onhowto enhance the i mmunogenicity of DNA vac-cine.It has been found in some research that thestrength of cellular i mmunity activity has things todo with the speed of antigen molecules’degrada-tion,i .e.the faster the degradation,the higher th… 相似文献