一种基于综合信息的剪接位点识别方法

为提高剪接位点识别的精度,提出一种基于综合信息的剪接位点识别方法.通过分析供体位点与受体位点的剪接信号、剪接序列、位点附近序列的二级结构,以及剪接因子作用过程等特征,分别为供体位点与受体位点建立信号模型和序列模型;应用Vienna软件中的Mfold包预测每个剪接位点附近序列最稳定的二级结构,将传统的四字符核酸表转化为八字符核酸表,每个序列用八字符进行描述,用结合了结构信息的序列对信号模型和序列模型进行训练学习;最后用训练好的模型进行剪接位点的识别.实验结果证明:该方法对剪接位点的识别取得了很好的效果,其识别精度可达95%以上.     

预测竞争性和非竞争性剪接位点对

基于剪接位点竞争机制,剪接位点对分成竞争性剪接位点对和非竞争性剪接位点对.并且竞争性和非竞争性剪接位点对的分类是一个很重要的工作.结合位置权重矩阵、离散量和支持向量机,提出了预测竞争性和非竞争性剪接位点对的新方法.独立检验集中90%以上的剪接位点对能被正确地分类成竞争性和非竞争性剪接位点对.此预测成功率高于其它方法.     

固化法布里-珀罗光纤传感器技术

装配和波阵面分裂固有发布里-珀罗光纤温度传感器。传感器将石英玻璃管接合到单模光纤尖端制作而成,其直径与光纤相同,长度不超过0.5mm。     

钢板在混凝土中锚固性能的试验研究

钢板在混凝土中锚固性能是保证预制拼接梁结构钢接头可靠工作的重要问题。本文通过预埋钢板的混凝土梁对比试验,着重研究了加焊抗剪栓筋钢板在混凝土中的锚固性能与受力机理,并提出了钢板锚固区计算方法。     

Study on Pneumatic Splice

An ideal pneumatic splice has not only interminglings in the middle, but also many small wrappings on both sides of the spliced region, and must have near overlapping loopy wrappings at both ends. Only the fluffy and hairy untwisted yarn ends obtained from a swirling jet untwisting can generate many small wrappings when spliced.To get the fluffy and hairy untwisted yarn ends, a small swirling intensity value X must be used to eliminate a reverse flow zone. To increase the wrappings at both ends, a larger swirling intensity value X must be used to form a reverse flow zone. Only a perfect splice can better the yarn's appearance,increase its strength and make it impossible for its fibers to slip easily.     

Theoretical Analysis of the Factors Affecting Splice Loss During Fabric Spreading

Material utilization is mainly concerned by industrialpractitioners since it contributes to minimize the totalproduction costs for garment manufacturing. Apart fromthe marker loss, splice loss (the fabric loss during thejoining of different fabric rolls) is one of the primarycauses of fabric loss, but it has not been considered spe-cifically in the past literatures. The amount of splice lossduring fabric spreading is governed by various factors in-cluding the marker length, the number of fabric rolls,and the number of splicing intervals for laying-up. Inour study, these three effects on splice loss during fabricspreading were computed and analysed.     

含铺层拼接层合板的等效刚度估算

通过分析铺层纤维取向和面内变形特点,提出纤维切断铺层拼接层合板的力学性能分析方法。按单层偏轴向力学性能转换关系,推导了不同纤维方向拼接层的等效工程弹性常数计算公式,并举例说明了含铺层拼接层合板的等效刚度计算方法。     

悬臂梁段拼接节点耗能的刚性钢框架时程分析

在带悬臂梁段拼接的刚性钢框架中,如果将梁的高强螺栓拼接节点设计得弱一些,可以利用其摩擦消耗地震能量文中首先根据已有的节点试验研究,提出该类拼接节点2种典型的滞回模型,并通过对试验构件滞回性能的数值模拟,验证所提出模型的有效性将该模型用于模拟框架结构中的拼接节点,利用SAP2000对框架结构进行弹塑性时程分析,研究耗能拼接节点对框架结构整体抗震性能的影响研究结果表明,利用拼接耗能的钢框架可以提高结构的耗能能力,从而改善结构的抗震性能     

Correction of RNA aberrant splice increases foreign gene expression in transgenic mice

Transgenic mice with mammary gland secreting human granulocyte colony stimulating factor (G-CSF) were produced using mice whey acid protein gene promoter. It was found that there was very low expression level in mammary gland. Human G-CSF cDNA was obtained by RT-PCR from transgenic mice mammary gland. Sequence analysis showed that this G-CSF gene deleted the 4th exon, and compared with human G-CSF genomic DNA, there were donor and acceptor splice sites in the deletion fragment. It was considered that the 3rd and 4th introns also delete in G-CSF fragment. The transgenic construct was corrected by deleting the 3rd and 4th introns to construct the minigene, which was used to produce transgenic mice by microinjection. Northern blot showed that G-CSF expression using the new construct increased 5.4 times as that before in transgenic mice. The results suggested that it was possible that RNA aberrant splice result in low expression in transgenic mice.     

基于快速傅里叶变换的剪接特征提取

挖掘剪接特征是剪接位点识别算法的基础,在频域空间挖掘对位点识别有帮助的特征至关重要.利用基于快速傅里叶变换的剪接特征提取方法对其进行特征提取,该方法能够将时域信息转化到频域中,以此来构建所需的频域特征,为了比较还构建了位置特征与统计特征. 实验结果表明将频域特征加入剪接位点识别中能够有效地提高识别精度,这也表明将信号处理方法应用于生物信息学领域是可行有效的.      

分形结构及尺度对粒子扩散行为的影响

土壤污染对人体健康和生态环境会产生极大危害.探明污染物扩散机理,准确预测污染物在土壤中的输运过程是高效控制和修复的前提.已有研究表明,土壤结构及尺度差异对污染物输运行为具有明显影响.为定量分析上述差异对预测结果的影响,在建立单块Sierpinski分形地毯模型的基础上,构建了四块拼接Sierpinski分形地毯模型模拟...     

梁铰区搭接节点抗震试验研究

通过对梁铰区采用纵向搭接的筋砼中节点的抗震性能试验研究。探讨了梁铰区搭接连接的可行性。重点分析了不同的搭接型式对梁铰区的弯曲延性、刚度退化，粘结锚固及耗能性能的影响，提出了此类节点最佳锚固型式及锚固长度的设计建设。     

模式生物基因序列的识别

真核生物的全基因组序列可分为三种:外显子、内含子和基因间序列.基于剪切位点附近序列的保守性,序列的组分特征和编码序列阅读框存在三周期性,三种序列的标准离散源由序列上64个三联体的概率和5′端与3′尾剪切位点附近(共30位点)上4个碱基的概率,共184个参数构成.某条序列的类型就可以由该序列的离散量与上面三个标准离散源的离散量之间的离散增量最小值决定.当标准离散源具有184个信息参数时预测率比64参数预测的成功率至少提高4.61%,前者的预测成功率依次如下:线虫88.37%,酵母菌90.72%,拟南芥91.08%,果蝇92.28%,大肠杆菌92.88%.对预测成功的和错误的两类序列进行比较,发现这些预测错误序列的184个参数值与其预测结果所属的那类序列本身的参数值十分类似.     

Pyrococcus sp.DNA聚合酶内蛋白子突变体的剪切性质

将含麦芽糖结合蛋白－嗜热菌ＤＮＡ聚合酶内蛋白子基因的ｐＭＩ８４、ｐＭＩ９４、ｐＭＩ９５三个重组突变体质粒转入Ｅ．ｃｏｌｉ２４２６经发酵及ＩＰＴＧ低温诱导表达,直链淀粉糖亲和纯化获得ＭＩ８４（ｓｅｒ１／Ｓｅｒ５３８Ｓｔｏｐ）、ＭＩ９４（Ｓｅｒ１→ＣｙｓＨ１／Ｓｅｒ５３８Ｓｔｏｐ）、ＭＩ９５（Ｓｅｒ１→Ａｌａ１／Ｓｅｒ５３８Ｓｔｏｐ）三种蛋白质前体。研究了ＰＨ温度、巯基试剂对内蛋白子Ｎ末端的剪切影响。     

拟南芥和线虫外显子/内含子剪切位点的研究

将拟南芥(A.tha liana)和线虫(C.eleg ans)的基因序列中的外显子按第一外显子,中间外显子和最后外显子划分成三类.分别将外显子/内含子剪切位点、翻译起始和终止位点附近的三联体的3个位点作为3条子链,以各条子链的不同碱基个数作为离散源参数,共12个离散源参数,计算各类外显子离散量.用离散增量实现了对三种序列类型的预测,预测成功率都达到80%以上;并且统计了剪切位点附近的碱基相对频数,结果比较了由于三联体所取位置及个数不同而造成的对预测结果的差异,说明了剪切位点附近碱基的保守性.     

两不同单模平面光波导的连接损耗与模场半宽之间的关系

模场半宽是单模光波导的重要参数,它与众多的传输特性相联系.对于圆对称单模光纤,文献提出了两个著名的模场半径,并发现它们与光纤的众多传输特性相联系.对于单模平面波导,文献提出了高斯最小二乘拟合模场半宽的定义,文献提出了两种矩定义模场半宽——近场二阶矩和Laplace模场半宽,并导出它们和横向偏移损耗和角向偏移损耗的关系式,对阶跃对称单模平面光波导的TE_0模的计算表明:当偏移量较小时,用矩定义模场半宽算得的损耗将比用高斯最小二乘拟合模场半宽算得的更准确.本文首先导出了任意两不同对称单模平面光波导在高斯近似下的横向偏移和角向偏移损耗公式,接着用矩定义模场半宽直接代替高斯模场半宽得到了矩定义与连接损耗的关系式,最后以阶跃对称单模平面光波导的TE_0模为例进行了大量计算,结果发现当偏移量较小时,用矩定义和高斯最小二乘拟合模场半宽算得的损耗误差均较小,并且若两光波导厚度相差不大,归一化频率及偏移量均较小时,用矩定义算出的连接损耗比高斯近似准确.     

A new method for splice site prediction based on the sequence patterns of splicing signals and regulatory elements

It is of significance for splice site prediction to develop novel algorithms that combine the sequence patterns of regulatory elements such as enhancers and silencers with the patterns of splicing signals. In this paper, a statistical model of splicing signals was built based on the entropy density profile （EDP） method, weight array method （WAM） and K test; moreover, the model of splicing regulatory elements was developed by an unsupervised self-learning method to detect motifs associated with regulatory elements. With two models incorporated, a multi-level support vector machine （SVM） system was devised to perform ab initio prediction for splice sites originating from DNA sequence in eukaryotic genome. Results of large scale tests on human genomic splice sites show that the new method achieves a comparative high performance in splice site prediction. The method is demonstrated to be with at least the same level of performance and usually better performance than the existing SpliceScan method based on modeling regulatory elements, and shown to have higher accuracies than the traditional methods with modeling splicing signals such as the GeneSplicer. In particular, the method has evident advantage over splice site prediction for the genes with lower GC content.     

单模光纤的接头损耗

对用OTDR测量光纤损耗进行了研究。结果表明，真正的接头损耗应由OTDR双向测试的平均值决定。由于光纤后向散射的变化，OTDR从一个方向测得的单向值并不代表真正的接头损耗，单向值大也不代表接头损耗大，在实际铺设光缆时，单向值高达0．5dB是可以接受的。     

大型板装配拼接节点试验研究

提出了装配整体式钢筋混凝土大型板一种新的横向拼接节点构造,对9根新型横向连接预制板试件进行试验研究与承载力分析,量测并分析试件的荷载、挠度及应变,观测试件的裂缝发展情况和破坏过程.试件的破坏形态包括弯曲破坏、受剪破坏及轴向拉伸破坏等形式.试验表明,该新型横向连接节点连接可靠,具有良好的承载能力和延性.对新型横向连接节点...     

空间铰接链杆体系几何组成性质分析的解析方法

采用解析方法．通过对结点位移性质的论证，提出了空间铰接链杆体系几何组成性质分析的解析方法．用此方法来分析网架、塔架及类似空间结构的几何组成，将会有十分有益的工程意义．