苜蓿花叶病毒复制酶基因全长cDNA植物表达载体的构建

将苜蓿花叶病毒中国分离株（Alfalfa mosaic virus Chinese isolate，AlMV-Ch）的复制酶P2亚基（90KD蛋白）基因的全长cDNA构建到植物表达载体pROK Ⅱ中，得到重组植物表达载体pAlMV-FL．     

六安地区高粱花叶病毒P3基因的克隆和序列测定

从六安产甘蔗病叶中提取总RNA,逆转录获得甘蔗花叶病毒(Sorghun mosaic virus,SrMV)基因组cDNA。设计P3基因特异引物,通过PCR扩增P3基因,并将P3基因cDNA克隆、测序。     

Increased viral pathogenicity after insertion of a 28S ribosomal RNA sequence into the haemagglutinin gene of an influenza virus

The haemagglutinin glycoprotein HA of influenza viruses is responsible for the attachment of the virus to neuraminic acid-containing receptors at the cell surface and subsequent penetration by triggering fusion of the viral envelope with cellular membranes. To express full activity of the newly synthesized precursor, HA has to be modified by post-translational proteolytic cleavage into the polypeptides HA1 and HA2 by cellular enzymes. If proteases suitable for cleavage are not present in the host cell, the resulting virus particles are non-infectious. During adaptation of the apathogenic influenza virus A/turkey/Oregon/71 to chicken embryo cells, which are not permissive for HA cleavage, we obtained an infectious virus variant with increased pathogenicity. Sequence analysis revealed that during adaptation 54 nucleotides were inserted into the HA gene; their sequence corresponds to a region of the 28S ribosomal RNA. This insertion is probably responsible for increased cleavability of HA, as well as for infectivity and pathogenicity of the adapted virus.     

Effects of metal ions and heat shock on the expression of a metallothionein-like gene htMT2 in Helianthus tuberosus

The expression of htMT2 in tissues of Helianthus tuberosus treated with metal ions and heat shock is studied by Northern blot hybridization. The results indicate that htMT2 is not expressed in roots. The expression in leaves and stems can be reduced by Cu2+ and induced by low concentration of Zn2+. However, the expression of htMT2 can be suppressed by Zn2+ when its concentrations are above 500 μmol/L. The effects of Ca2+ on the expression of htMT2 in leaves are similar to that of Zn2+. But Ca2+ induces the expression of htMT2 in stems. The expression levels of htMT2 in various tissues are not significantly affected by heat shock. The results above demonstrate that the expression of htMT2 can be regulated by metal ions and confirm that htMT2 is a new metallothionein-like gene.     

The study on the immune response induced by expressing recombinant plasmid of dengue virus type 2 NS3 protein

The PSV·NS3, an expressing recombinant plasmid of dengue virus type 2 NS3 protein, was injected directly into the quadriceps of Balb/C mice to explore whether it could inducing immune response. The splenic T cell subsets of two groups was analysed by flow cytometry. It was found that the percentage of CD4⁺ and CD8⁺ T cells of experimental group were significantly higher than those of the control group. The titer of IgG antibody was as high as 1∶5 120 in experimental group, but it couldn’t be detected in control group by ELISA. The western blot further proved that the IgG antibody was specific for NS3 protein. Those results Suggested that inoculation Balb/C mice with PSV·NS3 could inducing immune response, and the NS3 protein might be used as the candidate protein of DNA vaccine of dengue virus. Foundation item: Supported by Foundation of American Chinese Medicine Biography, LI Xiang-qun(1963−), male, M.D, Research direction: molecular and clinical virology.     

首例t（8;20）易位的M2—ANLL白血病重组位点的初步研究

对临床上确认为Ｍ２－ＡＮＬＬ的病例，首先进行骨髓细胞染色体的核型分析，经Ｇ显带和Ｒ显色确定为ｔ（８；２０）（ｑ２２；ｐ２３）易位，是一种新的变异型，为进行分子生物学研究，分别设计引物，反转录扩增检测ＥＴＯ基因ｃＤＮＡ ３‘０端顺序和ＥＴＯ融合转录物检测为阴性，从分子水平上排除了经典的ｔ（８，２１）易位的可能性。说明了该病例为ｔ（８；２０）易位，ＥＴＯ基因受累的新变异型白血病。     

CTAB对血红蛋白仿过氧化物酶催化活性的影响

本文研究了表面活性剂介质存在下,血红蛋白作为过氧化物模拟酶,以邻苯二酚供氢体底物的催化特性和反应条件,并探讨了表面活性剂对酶催化反应的影响及可能机理,稳态速率法测定了米氏常数（Km）、米氏速率（Vm）及反应级数等动力学参数。试验表明本体系在阳离子表面活性剂CTAB存在和pH10.3的条件下形成的产物在258nm处有最大的吸收峰。     

板钛矿和锐钛矿TiO2对水热合成Bi4Ti3O12微结构的影响

分别以板钛矿和锐钛矿型的TiO2为先驱物水热合成钛酸铋亚微米棒.由X射线衍射、选区电子衍射以及Raman光谱的测量结果可知,合成的产品为Bi4Ti3O12. 源自板钛矿和锐钛矿的Bi4Ti3O12,其结构表现出明显的差异.在同样的条件下,Bi插入锐钛矿TiO2的量多于其插入板钛矿TiO2的量,从而导致部分X射线衍射峰向高角度方向稍微移动.保留下来的短Ti-O键限制了b-Bi4Ti3O12亚微米棒沿[010]方向生长,使得b-Bi4Ti3O12亚微米棒长度不均.