日本脑炎病毒（JEV）内蒙古分离株M73—1E基因的5′端？…

根据国外报道的ＪＥＶ（Ｊａｐａｎｅｓｅ ｅｎｃｅｐｈａｌｉｔｉｓ ｖｉｒｕｓ）基因组全序列,针对ＪＥＶ Ｅ基因５′端设计合成一对特异引物,以日本脑炎病毒内蒙古分离株Ｍ７３－１ＲＮＡ为模板,经ＲＴ－ＰＣＲ扩增,获得３６６ｂｐ的ＪＥＶ Ｅ基因ｃＤＮＡ片段。将此片段重组于质粒ｐＵＣ１９中,并转化大肠杆菌ＤＨ５α,经ＰＣＲ扩增,酶切及序列分析,结果表明ＪＥＶ Ｍ７３－１ ５′端１２６个核苷酸序列与ＪＥＶ日     

高输入阻抗低噪声JFET的研究

高输人阻抗低噪声ＪＦＥＴ的研究冯景星（福州大学电子科学与应用物理系，福州，３５０００２）１理论分析１．１漏电流ＩＤ与同源电压ＶＤＳ、栅源电压ＶＧＳ的关系１）由ＪＦＥＴ的工作原理，从理论上可推导得ＩＤ、ＶＤＳ、ＶＧＳ之间的关系为：式中：由（１）式可得跨...     

THE ORBITAL EVOLUTION OF THE NEAR-EARTH ASTEROIDS

ＴＨＥＯＲＢＩＴＡＬＥＶＯＬＵＴＩＯＮＯＦＴＨＥＮＥＡＲ┐ＥＡＲＴＨＡＳＴＥＲＯＩＤＳＬｉｕＬｉｎＪｉＪｉａｎｇｈｕｉ（ＤｅｐａｒｔｍｅｎｔｏｆＡｓｔｒｏｎｏｍｙ，ＮａｎｊｉｎｇＵｎｉｖｅｒｓｉｔｙ，２１００９３，Ｎａｎｊｉｎｇ，ＰＲＣ；）Ｋｅｙｗ...     

甜菜坏死黄脉病毒新疆分离物外壳蛋白基因的原核表达载体和植物表达载体的构建

将克隆入ｐＧＥＭ７ｚｆ（＋）的甜菜坏死黄脉病毒（ＢＮＹＶＶ）新疆分离物外壳蛋白（ＣＰ）基因的ｃＤＮＡ的ｐＧＥＢ３，用ＸｂａＩ切下，Ｋｌｅｎｏｗ补平，再用ＢａｍＨＩ切下ｃＤＮＡ片段。用ＮｄｅＩ切开原核表达载体ｐＪＷ２，用Ｋｌｅｎｏｗ补平，再用ＢａｍＨＩ切去小片段。将该ｃＤＮＡ与ｐＪＷ２大片段用Ｔ４连接酶连接，构建了ＢＮＹＶＶＣＰ基因的表达载体ｐＪＷＢ４，转化到大肠杆菌ＤＨ５α。经培养和高温诱导，ｐＪＷＢ４成功地表达出了ＢＮＹＶＶ的外壳蛋白。将ｐＧＥＢ３和ｐＢＩ１２１用Ｘｂａｌ和ＢａｍＨＩ酶切Ｔ４连接酶连接，构建了ＢＹＶＶＣＰ基因的植物表达载体，转化到ＤＨ５α，筛选出正向连结的阳性克隆ｐＢＩＢ３，转化入农杆菌ＬＢＡ４４０４（ｐＡＬ４４０４），经用ＰＣＲ扩增和γ３２Ｐ标记的探针杂交约证实为阳性克隆。往甜菜植株中转化工作正在进行中。     

用JAVA/CORBA构筑新型的企业分布开放系统

ＪＡＶＡ的可下载对象功能和它与ＷＷＷ浏览器的紧密结合,使ＪＡＶＡ成为开发基于Ｉｎｔｅｒｎｅｔ和ＷＥＢ的分布式应用的理想媒体,但由于ＪＡＶＡ缺乏Ｃｌｉｅｎｔ／Ｓｅｒｖｅｒ（客户／服务器）模型,必须通过增加一个分布对象基础结构来弥补,ＯＭＧ（对象管理组）的ＣＯＲＢＡ解决了ＪＡＶＡ在Ｃｌｉｅｎｔ／Ｓｅｒｖｅｒ的缺陷,通过ＪＡＶＡ和ＣＯＲＢＡ的结合,可以构筑新型的企业分布开放系统。     

视频压缩编码的国际标准

为了使视频通信能更有效地利用信道带宽和存储空间，必须对视频数据进行压缩编码．在介绍了视频压缩编码的基本方法以后，着重叙述应用范围从静止图像到ＨＤＴＶ的国际视频编码标准或方案，其中包括ＪＢＩＧ，ＪＰＥＧ，Ｈ．２６１，ＭＰＥＧ和ＨＤＴＶ等．最后，对视频压缩领域的应用现状和前景作了一些分析．     

一类边值问题的解集结构

本文给出了一类边值问题的唯一性及解集的结构，同时，对ＰＢＶＰ和ＢＶＰ，证明了当右端函数ｆ关于ｕ单调不减时，上，下解的存在性等价于解的存在性，其主要结果改进和推广了Ｖ．Ｌａｋｓ，Ｅ．Ｌｉｚ和Ｊ．Ｊ．Ｎｉｅｔｏ等人的相应结果。     

发展浙江近海增养殖渔业的若干建议

发展浙江近海增养殖渔业的若干建议严建培（浙江省科技情报研究所杭州３１０００６）ＩＤＥＡＳＯＮＴＨＥＤＥＶＥＬＯＰＭＥＮＴＯＦＣＯＡＳＴＡＬＡＯＵＡＣＵＬＴＵＲＥＯＦＺＨＥＪＩＡＮＧＰＲＯＶＩＮＣＥＹａｎＪｉａｎｐｅｉ（ＺｈｅｊｉａｎｇＳｃｉｅｎｃｅ－...     

利用JAVA编程实现网络安全通信

网络安全涉及面极广，而数据传输的准确性，机密数据的安全性是计算机网络安全通信的关键。在ＩＮＴＥＲＮＥＴ网络环境下，讨论利用ＪＡＶＡ语言实现网络安全通信的编程特性，着重说明基于ＴＣＰ／ＩＰ协议的ＪＡＶＡ．Ｎｅｔ，Ｓｏｃｋｅｔ类库的通信机制，以及客户机／服务器工作模式。     

THE OPTICAL OBSERVATION OF THE COLLISTIONS OF COMET SHOEMAKER－LEVY 9 WITH JUPITER

ＴＨＥＯＰＴＩＣＡＬＯＢＳＥＲＶＡＴＩＯＮＯＦＴＨＥＣＯＬＬＩＳＴＩＯＮＳＯＦＣＯＭＥＴＳＨＯＥＭＡＫＥＲ－ＬＥＶＹ９ＷＩＴＨＪＵＰＩＴＥＲ￥ＺｈｏｕＨｏｎｇｎａｎ（ＤｅｐａｒｔｍｅｎｔｏｆＡｓｔｒｏｎｏｍｙ，ＮａｎｊｉｎｇＵｎｉｖｅｒｓｉｔｙ，２１...     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with