Effects of angiotensin II and of an angiotensin II receptor antagonist on Simian virus 40-induced tumor growth in vivo

Summary The effects of angiotensin II and of the competitive angiotensin II receptor antagonist saralasin on in vivo tumor growth were investigated in hamsters. Angiotensin II strongly inhibited tumor growth while saralasin stimulated it, though the high dose used had partial agonistic angiotensin II-like actions. Lower doses of saralasin were without significant effect on tumor weights.Acknowledgments. These studies were supported by the Deutsche Forschungsgemeinschaft (DFG) within the SFB 90 Cardiovaskuläres System. Saralasin was a gift from Dr A.W. Castellion, Norwich Pharmacal Company, USA. We thank S. Thiele, P. Baumgärtl and D. Kurz for excellent technical assistance.     

Serum activity inhibiting specific simian virus 40-induced transplantation resistance and its correlation with primary SV40 tumors appearance in hamsters

Summary Using the modified technique of transplantation test, ITR serum activity was found in most (14 out of 21) individual hamster sera obtained during the latent period of primary SV40 carcinogenesis (60 days after virus infection when newborn). On the other hand, as a rule, no ITR activity was observed in the sera of the same hamsters after tumor appearance and during their growth. ITR activity rapidly disappeared from sera of hamsters neonatally infected with SV40 after their successful immunization with the same virus during the latent period. There appears to be a correlation between the presence of ITR serum factor during the latent period and the subsequent primary SV40 tumor appearance in hamsters.Acknowledgment. The author wishes to thank Dr Galina I. Deichman for continuing advice and encouragement during the course of these studies.     

Serum activity inhibiting specific simian virus 40-induced transplantation resistance and its correlation with primary SV40 tumors appearance in hamsters.

Using the modified technique of transplantation test, ITR serum activity was found in most (14 out of 21) individual hamster sera obtained during the latent period of primary SV40 carcinogenesis (60 days after virus infection when newborn). On the other hand, as a rule, no ITR activity was observed in the sera of the same hamsters after tumor appearance and during their growth. ITR activity rapidly disappeared from sera of hamsters neonatally infected with SV40 after their successful immunization with the same virus during the latent period. There appears to be a correlation between the presence of ITR serum factor during the latent period and the subsequent primary SV40 tumor appearance in hamsters.     

Effects of intracerebroventricular injections of angiotensin II on prolactin plasma levels in the Rhesus monkey

Summary Intracerebroventricular injections of angiotensin II (A II) induced a sharp and dose-dependent increase in plasma prolactin (PRL) levels in the awake Rhesus monkey. Our results suggest that in primates, A II may be involved in the mechanisms controlling PRL secretion.Acknowledgments. We thank Mrs. J. Arsaut and D. Verrier for excellent technical assistance. This work was supported by grants from INSERM (CRL No. 78.1.2656), DGRST (77.7.9654) and CNRS (ERA 493).     

Failure of somatostatin to influence experimental tumor cell growth in vivo and in vitro

The influence of somatostatin on tumor cell growth was studied in vivo in mice (sarcoma 180 ascites tumor and Lewis lung tumor) and in vitro on nontransformed and polyoma-transformed cell lines. 4 or 20 micrograms/100 g of cyclic somatostatin and 4 micrograms/100 g of linear protamin Zn-bound somatostatin were injected s.c. twice daily in the in vivo study. Cyclic somatostatin (1, 4 or 10 micrograms/ml) was added twice daily to the cell cultures. Somatostatin administration influenced neither the survival of animals nor the growth rate of cultured cell lines.     

Failure of somatostatin to influence experimental tumor cell growth in vivo and in vitro

Summary The influence of somatostatin on tumor cell growth was studied in vivo in mice (sarcoma 180 ascites tumor and Lewis lung tumor) and in vitro on nontransformed and polyoma-transformed cell lines. 4 or 20 g/100 g of cyclic somatostatin and 4 g/100 g of linear protamin Zn-bound somatostatin were injected s.c. twice daily in the in vivo study. Cyclic somatostatin (1, 4 or 10 g/ml) was added twice daily to the cell cultures. Somatostatin administration influenced neither the survival of animals nor the growth rate of cultured cell lines. *** DIRECT SUPPORT *** A2025117 00011     

Effects of angiotensin II and epinephrine infusion on juxtaglomerular cell granularity and arterioles of the rat kidney

Zusammenfassung In Ratten wurde durch Injektion von Angiotensin II und Epinephrin akuter systemischer Hochdruck erzeugt. Angiotensin II verursachte Kontraktion der kleinen Arterien mit Zylindern in distalen Abschnitten und Sammelröhrchen. Der Juxtaglomeruläre Index (JGI) war leicht erhöht (p < 0.02). Epinephrin verursachte erhebliche Hyperämie und Dilatation der kleinen Arterien; der JGI war etwas erniedrigt (p < 0,1). Die Ergebnisse zeigen, dass, obwohl Angiotensin II und Epinephrin hochaktive Vasokonstriktoren sind und auch erhöhten Blutdruck aufrechtzuerhalten vermögen, sie verschiedene Effekte auf individuelle Gefässbereiche haben.

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This investigation was supported by U.S.P.H.S. Grant No. HE 06975 of the National Heart Institute.     

Effects of phenethyl alcohol on influenza virus growth and macromolecular synthesis in chick embryo cells

Zusammenfassung Phenethylalkohol wirkt virusstatisch und es wird vermutet, dass die mit der Zellmembran in Beziehung stehenden Vorgänge der Zellvermehrung beeinflusst werden.     

Changes in immune reactivity during growth of an adenovirus 12-induced transplantable tumour in CBA mice

Summary Early suppression, followed by a period of enhancement and finally, suppression, was seen when the spleen cell response to T and B cell mitogens was monitored during growth of an adenovirus 12-induced tumour in CBA black mice. The macrophage content of the tumour changed with time and these fluctuations correlated with the ability of tumour tissue extracts to enhance the normal spleen cell response to mitogen.Acknowledgments. Thanks are dur to the Yorkshire Cancer Research Campaign, and the Cancer Research Committee of Sheffield University for financial support, also to Professor C.W. Potter for advice.     

Enhancement of tumor development in physically-stressed mice inoculated with an oncogenic virus

Riassunto L'applicazione di un parziale bendaggio nei topi produce uno stato di fisiologica tensione caratterizzata, in parte, dall'involuzione della ghiandola del timo. Topi sottoposti a una tale tensione sono più suscettibili allo sviluppo dei tumori quando vengono inoculati con un oncogenico virus.     

Sodium concentration and the effect of angiotensin II on iieal smooth muscle

Zusammenfassung Die kontraktile Reaktion des isolierten Meerschweinchen-Ileums auf Angiotensin II variierte direkt mit der Natriumkonzentration der Nährflüssigkeit, nicht aber bei ACh; Atropin (10^–6 g/ml) reduzierte die Wirkung auf Angiotensin um 60–70% und hob die Wirkung auf äquivalente Mengen von ACh auf. In atropinhaltiger Ringerlösung potenzierte ein hoher Natriumgehalt (+ 30 meq./l) die Reaktion auf Angiotensin zehnfach.     

Effect of cortisol and norethandrolone on inflammation and tumor growth

Résumé Le norethandrolone administré chez le rat intact ou surrénalectomisé se montre très efficace à prévenir la perte de poids causée par le cortisol. Cet antagonisme, toutefois, n'est pas démontrable en ce qui concerne l'effet anti-inflammatoire, la diminution de la croissance tumorale et l'atrophie du thymus et de la surrénale dus au cortisol. En outre, le norethandrolone présente à lui seul des propriétés anti-inflammatoires et thymolytiques, plus spécialement chez l'animal intact.

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This work was supported by grant from the National Cancer Institute of Canada. The authors wish to thank Pfizer Canada, Montreal, and G. D. Searle & Co., Chicago, U.S.A. who graciously supplied cortisol acetate and norethandrolene.

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Medical Research Associate of the National Research Council of Canada.

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Work done under tenure of a summer fellowship from the Fondation Rhéaume of Montreal (Canada).