东北红豆杉愈伤组织诱导及组织培养研究

东北红豆杉扦插苗幼茎愈伤组织的诱导频率B5－B组（86．7％）＞MS－A组（60．2％）＞MS－B组（37．0％）。MS培养基中2，4－D和NAA同时存在时，愈伤组织诱导效果比不含NAA的要好1．5－2倍。MS培养基是 愈伤组织生长的适宜培养基。经过几代的继代培养，愈伤组织的生长周期由35－40d缩短为15－18d。红豆杉树与扦插苗间、树的不同个体间，甚至同一树不同部位的幼茎在愈伤组织诱导及生长方面都存在较大差异。     

谷氨酰胺在小麦花药培养中的作用之探讨

以Ｃ１７为基本培养基,研究分析了在诱导培养基中添加不同浓度的谷氨酰胺对小麦花药愈伤诱导的影响效应。三年来多个基因型的试验结果表明,诱导培养基中添加谷氨酰胺对小麦花药愈伤诱导没有促进效应,这可能是和添加的谷氨酰胺与培养基中的亮氨酸在进入三羧酸循环时产生竞争抑制有关。     

蒲公英试管培养中芽形成的研究

应用组织培养技术,探讨不同外植体、生长调节剂及pH对蒲公英芽诱导形成的影响。结果表明：以茎尖作为外植体芽诱导率最高,可达95％,35d时,每个外植体所产生的芽数达5个以上,叶片次之,茎段较低;MS培养基中添加0．05—5mg/L6-BA或KT,对芽形成具有促进作用,其中6-BA2mg/L的用量效果最优,浓度大于5mg／L则抑制芽形成;6-BA和KT分别与NAA或IAA结合使用时比单独使用6-BA和KT效果更为显著,其最佳的浓度比例为2：1;最优组合为2mg/L6-BA 1mg/L IAA;pH以5．8为宜。     

Regulation of protein synthesis, intracellular electrolytes and cataract formation in vitro

Control of protein synthesis is associated with changes in the ratio of intracellular Na+ to K+ in the cultured embryonic chick lens. Correlations of intracellular Na+/K+ ratios with crystallin synthesis and cataract formation in vitro suggest that the Na+/K+ ratio may have an important role in the regulation of protein synthesis during cataractogenesis.     

满天星试管苗的生根培养

本实验研究了适于满天星试管苗生根的基本培养基和生长素的种类，浓度．结果表明：l／2MS培养基为生根的最适基本培养基，在培养基中加入0．5mg／L的NAA最有利于根的分化和生长．     

噻二唑苯基脲在平贝母组织培养中的作用

以平贝母鳞片和黄化幼茎为外植体作组织培养,进行了噻二唑苯基脲等４种因素的正交试验。结果表明：ＴＤＺ在浓度很低时便具有很强的细胞分裂素活性,可刺激平贝母外植体脱分和分化,抑制生根;生长素ＮＡＡ在平贝母组织培养中起着比细胞分裂素更为重要的作用;在ＭＳ,ＭＮ和Ｎ６ ３种高盐培养基上,诱导结果无显著差异,但ＭＳ对鳞片离体培养相对较好,Ｎ＾对黄化幼茎培养较佳。     

不同激素及添加物对杭白菊组织培养的影响

以杭白菊茎尖为试材,将其接种在培养基上（以NAA,AC,2,4-D,6-BA及水解乳蛋白为5因素进行正交,设计16组培养基）进行愈伤诱导及出芽,探讨不同添加剂对杭白菊组织培养的影响,同时分析不同因素之间影响的显著性．结果表明,不同种类浓度配比对杭白菊组织培养会产生较大影响,最佳愈伤培养基组成为MS＋3mg／L6-BA＋0．2mg／LNAA＋0．8mg／L2,4-D＋0．5％AC＋0．2％水解乳蛋白＋0．8％琼脂＋3％蔗糖;出芽培养基最佳组成为MS＋1mg／L6-BA＋0．5％AC＋0．8％琼脂＋3％蔗糖．     

Glucocorticoid hormones inhibit DNA synthesis and enhance interferon production in human lymphoid cell line

Although buffy coat leukocytes have been the prime source of human interferon, cells of the Burkitt lymphoma line Namalwa are increasingly used for the large scale production of interferon. On induction with Sendai virus or Newcastle disease virus, Namalwa cells produce a substantial quantity of interferon which contains predominantly the leukocyte antigenic species and minor amounts of fibroblast-type interferon. We have recently demonstrated that inducers of erythropoietic differentiation in Friend cells are able to enhance interferon synthesis in Namalwa cells when added to cultures larger than or equal to 24 h before interferon induction by Sendai virus. The most potent compounds, n-butyrate, stimulated interferon production about 30-fold and has also been independelty described by others. All active compounds inhibited DNA synthesis in Namalwa cells and the extent of inhibition apparently paralleled the stimulatory potency of the respective compound. Induction of differentiation of Friend cells can be antagonised by various steroid hormones, which by themselves have no measurable effects on these cells. In contrast, we report here that glucocorticoid hormones inhibit DNA synthesis in Namalwa cells and augment Sendai virus-induced interferon synthesis.     

不同培养条件对金钗石斛悬浮培养细胞生长的影响

研究了培养温度、培养时间、初始p H、摇床转速对金钗石斛液体悬浮培养的影响。结果表明,在培养条件分别为培养温度为25℃、培养天数为30d、初始p H为6、摇床转速为120r/min时,金钗石斛原球茎增殖量最大,石斛碱的积累量也相对最高。