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1.
以杜仲(EncommiaulmoidesOliver)当年生枝条的叶片、带芽茎段为外植体,基本培养基为MS,附加不同浓度的BA、KT和NAA.最佳芽诱导培养基为:MS KT1 0mg/L NAA0 1~0 5mg/L;MS BA2 0mg/L NAA0 1~0 5mg/L,最佳芽丛增殖培养基为:MS KT2 0mg/L NAA0 1~0 2mg/L;MS BA1 0~2 0mg/L NAA0 1~0 2mg/L;MS KT2 0mg/L IBA0 1~0 2mg/L;MS BA1 0~2 0mg/L IBA0 1~0 2mg/L,单芽增殖培养基:MS KT1 0mg/L NAA0 1mg/L,生根培养基:13MS KT0 1mg/L NAA0 5mg/L.  相似文献   

2.
新铁炮百合组织培养和快速繁殖研究   总被引:35,自引:4,他引:35  
以日本新铁炮百合鳞茎及叶为外植体成功获得再生植株,并建立了快速无性繁殖系。鳞片和叶的最佳诱导培养基分别为MS+0.4-1mg/L BA 0.2mg/L NAA;MS 0.1mg/L(或0.5mg/L)BA+0.1mg/L NAA或0.5mg/L IBA。芽增殖培养基选MS+0.2-0.5mg/L BA 0.1mg/L NAA或0.2mg/L IBA。生根培养基为1/2MS+0.1mg/L IBA 0.1%活性炭。  相似文献   

3.
以粉蕉吸芽为材料开展茎尖离体快速繁殖研究,结果表明:以MS+BA3-5mg/l(单位不同)+NAA0.2为诱导培养基可以获得不定芽;以MS+BA3.0+Ad10.0为继代培养基可以获得较高的不定芽增殖率且变异率极低,后期附加一定量的NAA有助于促根;生根培养以MS+IBA1.0-2.0+NAA0.1+Ac1为宜。  相似文献   

4.
在附加激素的MS培养基上,培养大叶白麻消毒茎切断,诱导产生愈伤组织和丛生芽。经过继代培养,建立了大叶白麻无性繁殖系,实验结果表明:1)培养基MS+6BA1mg/L+NAA2mg/L愈伤组织诱导,MS+6BA1mg/L+NAA0.2mg/L及MS+6BA2mg/L诱导外殖体产生丛生芽和继代培养,1/2+IAA0.2mg/L诱导生根效果最好。  相似文献   

5.
食荚型豌豆组织培养和植株再生研究   总被引:1,自引:0,他引:1  
以豌豆子叶、茎、真叶为材料进行外植体筛选及组织培养研究,试验结果表明:豌豆带节茎段是最佳试验材料;在MS附加1 mg/L BA和1 mg/L NAA培养基中,愈伤组织诱导率达100%,愈伤组织在分化培养基中未分化出芽;带节茎段在MS附加2~3 mg/L BA和0.1 mg/L NAA培养基中,腋芽和丛生芽产生率达100%,芽增殖系数3以上;不定芽在MS附加3 mg/L NAA上生根率86%;试管苗移栽成活率85%左右.  相似文献   

6.
兰州百合和野百合组织培养及快速繁殖研究   总被引:42,自引:1,他引:42  
以兰州百合(Lilium davidii Var.Unicolor (Hoog)Cotton)和野百合(Lilium brownii F.E.Brown ex Miellez) 鳞茎及叶片为外植体获得再生植株,并建立起快速无性繁殖系。兰州百合鳞茎的最佳诱导培养基为MS+0.6-1.0mg/LBA+0.2mg/LNAA;芽增殖培养基选MS+0.5-0.6mg/LBA 0.1mg/L NAA;生根培养基为1/2MS+0.2mg/l IAA 0.1%活性炭。野百合鳞茎及叶的最佳诱导培养基分别为MS+0.1-0.5mg/LBA 0.1-0.2mg/L NAA或0.5mg/L IAA和MS+0.7mg/L BA 0.07mg/L NAA;鳞茎诱导的芽的增殖培养基为MS+0.2-0.4mg/L BA 0.1mg/L NAA;生根培养基为1/2MS+0.15mg/L NAA 0.1%活性炭。  相似文献   

7.
库拉索芦荟(Aloe vera L.)、中国芦荟[A.vera L.var.chinensis(Haw.)Berg.]、上农大叶芦荟(A.vera L.var SAC Gu.)三种芦荟的茎段和顶芽外植体在MS附加BA3-4mg/L的培养基上培养,产生大量不定芽,不定芽长大后,在MS附加IBA1-2mg/L NAA 0.2mg/L的培养基上生根并形成完整植株。生根试管苗经练苗后移入沙床,生长良好。  相似文献   

8.
将油莱0guCMS与诸葛菜属间杂种田交一代(BC1),在MS附加不同激素浓度的基本培养基上诱导丛生芽.结果发现,MS+1.5mg/L 6—BA 0.15mg/L NAA对诱导丛生芽有较好的效果,丛生芽增殖系数可达3.2.小芽生根培养基以MS 0.1mg/L NAA的效果较好.  相似文献   

9.
凤仙花茎段培养与快速繁殖   总被引:2,自引:0,他引:2  
试验以茎段为外植体,进行凤仙花离体再生与快速繁殖技术研究.结果表明:在附加BA1.0mg/L、NAA0.2mg/L、3%蔗糖的MS固体培养基上可诱导茎段外植体直接产生大量不定芽,诱导率达100%;在含BA1.0mg/L、N从0.2mg/L、3%蔗糖的MS培养基上进行继代培养,可大量增殖;在含NAA、1.0mg/L。3%蔗糖的1/2MS培养基中长根成苗.本试验表明风仙花离体繁殖,增殖系数大,适合于种苗快速繁殖.  相似文献   

10.
福建省山樱花的组织培养及植株再生   总被引:5,自引:0,他引:5  
以春季萌发的福建山樱花嫩枝为外植体诱导丛生芽 。在1/4MS+BA1.0mg/L IBA0.1mg/L的培养基上,其丛生芽诱导率达87.5%;在继代培养中选择MS+BA1.0mg/L IBA0.1mg/L GA30.3mg/L或KT1.0mg/L NAA0.1mg/L BA30.3mg/L培养基,不定芽增殖较快。赤霉素对其不定芽的伸长有明显效果,当芽伸长在3-4cm时,切下置于生根培养基1/2MS+NAA1.0mg/L IBA1.0mg/L BA0.75mg/L中,生根率达90%以上,移栽成活率达95%。  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

13.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

14.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

15.
正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-  相似文献   

16.
We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-  相似文献   

17.
正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that  相似文献   

18.
<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with  相似文献   

19.
20.
<正>"Journal of Jilin University(Science Edition)"is a comprehensive academic journal in the fields of science sponsored by Jilin University and administrated by the Ministry of Education of the Peoples Republic of China.The journal started publication in 1955.The original name at starting publication was"Journal of Natural Science of Northeast People University",which was changed into"Acta Scientiarum Naturalium Universitatis Jilinensis"in 1958 owing to the name change of the university.The present journal name has  相似文献   

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