Adhesion of subsets of human blood mononuclear cells to porcine endothelial cells

Cellular immune response is a major barrier to xenotransplantation, and cell adhesion is the first step in intercellular recognition. Flow-cytometric adhesion assay has been used to investigate the differential adhesions of monocyte (Mo), natural killer cell (NK) and T lymphocyte (T) present within human peripheral blood mononuclear cells (PBMC) to porcine aortic endothelial cells (PAEC), and to demonstrate the effect of human interferon-γ (hIFN-γ) or/and tumor necrosis factor-α (hTNF-α) pretreatment of PAEC on their adhesiveness for different PBMC subsets. The preferential sequence for PBMC subset binding to resting PAEC is Mo, NK and T cells, among which T cells show the slightest adherence; hTNF-α can act across the species, and augment Mo, NK and T cell adhesion ratios by 40%, 110% and 3 times, respectively. These results confirm at the cell level that host Mo and NK cells are major participants in the cellular xenograft rejection, thereby, providing a prerequisite for further studying the human Mo/NK-PAEC interactive mechanisms.     

The time course of visual categorization： Electrophysiological evidence from ERP

Categorization is the process to ascertain the identity of an object by a specific standard[1,2]. Categorization isa fundamental cognitive process; in fact, the raw per- ception would be of little use without the effective clas- sification of original inf…     

Synthesis and micellization behavior of stimuli-responsive polypeptide hybrid triblock copolymer

Polypeptide hybrid triblock copolymer, poly（L-glutamic acid）-b-poly（propylene oxide）-b-poly （L-glutamic acid） （PLGA-b-PPO-b-PLGA）, was synthesized by the ring-opening polymerization of benzyI-L- glutamic N-carboxyanhydride （BLG-NCA） using poly（propylene glycol） bis（2-aminopropyl ether） as initiator, followed by the subsequent deprotection step. The obtained double hydrophilic triblock co- polymer exhibits ＂schizophrenic＂ micellization behavior in aqueous solution upon dually playing with solution pH and temperature. The multi-responsive micellization behavior of this polypeptide hybrid triblock copolymer has been thoroughly investigated by 1H NMR, laser light scattering （LLS）, tempera- ture-dependent optical transmittance, and circular dichroism spectroscopy （CD）.     

ABT3生根粉处理在油棕大树移栽的应用

在油棕大树移植中,用50~150 m g.L-1的ABT 3号生根粉溶液灌土球移栽,结果表明:可以促进移栽大树的早期生长,新根出土球数和新叶数都显著高于同期用常规方法移栽的植株,尤其是以处理B为后期生长打下良好基础。其中以100 m g.L-1灌土球处理的效果最佳。     

在井下温度合成瓦斯水合物的实验

通过实验研究了利用模拟瓦斯气体在井下温度和中高压的条件下合成瓦斯水合物。采用以实验为主，理论分析和计算机模拟相结合的研究方法，目的在于找出压力、温度及表面活性剂对水合物生成过程的影响。在井下温度和中高压的条件下，采用合适的表面活性剂及一定配比成功合成了瓦斯水合物。得出的结论是：表面活性剂在溶液中形成胶束后很大程度上可增加瓦斯气体的溶解度；与压力相比。温度对水合物的影响更重要：在气一液界面先合成的水合物有利于剩余水合物的合成。     

Identification of selenocysteine insertion sequence (SECIS) element in eukaryotic selenoproteins by RNA Draw program

The computer program RNA Draw was used to identify the secondary structures in the 3′ untranslated regions (3′UTRs) of the mRNAs from 46 eukaryotic selenoproteins among 7 species. The program found one or two possible SECIS elements in these selenoproteins. The SECIS element consists of a stem-loop or hairpin structure with three conserved sequences of AUGA-(A)AA-GA. SECIS element was not found by the RNA Draw program in randomly selected non-selenoproteins. The results showed that SECIS element is the unique character of the genes of eukaryotic selenoproteins. Thus it is possible to use RNA Draw to search the SECIS elements in gene bank for potential new selenoproteins.     

混合动力汽车电池管理系统SOC的评价

为建立混合动力汽车电池管理系统的需要，探索镍氢电池荷电状态(SOC)的实时测量和估计方法，分析当前一般SOC定义在变电流放电情况下出现不适应的原因，和现有各种荷电状态估计方法存在的问题。为此，根据能量守恒原理，提出了一种新SOC的概念，使之能很好地适应混合动力汽车用电池在变电流状态下的实时荷电状态估计，并且基于新的SOC定义，建立电池荷电状态计算模型，进行仿真分析，简化计算，明确物理意义，提高了SOC的判断精度，减少混合动力汽车的复杂性，减少整车的成本，为混合动力汽车系统优化匹配提供了依据。     

Isolation and purification of BVⅠ-2H from bee venom and analysis of its biological action

The medical use of bee venom for rheumatoid arthritis (RA) has a very long tradition. In this study, isolation and purification of polypeptides from bee venom were carried out on sephadex chromatography, heparin sepharose CL-6B chromatography and HPLC. Several fractions were extracted, and their effects on activation of splenocyte and THP-1 cell were studied. The inhibitory fraction was selected for further studies. Finally, BVⅠ-2H that the HPLC elution profiles was a single peak was isolated by C8 column. ESI- MS detection results showed that BVⅠ-2H was a fraction of bee venom, and the molecular weight of the major component was 644.8. BVⅠ-2H could inhibit ConA-induced splenocyte proliferation, IL-1 production and interfere with splenocyte cycle in mice. Moreover, BVⅠ-2H could inhibit PMA-induced TNFa production in THP-1 cells, which was due to its inhibitory effects on TNFa mRNA expression and protein phosphorylation of IkBa. Our studies indicated that BVⅠ-2H was one of the anti-inflammatory components of bee venom.     

SDL-2000动态语义定义方法

讨论了SDL-2000的建议Z．100的附件F中用分布式实时抽象状态机(ASM：Abstract State Machine)形式地定义SDL(Specisification and Descripiton Language)动态语义的方法。与基于通信顺序进程的方法相比，该方法能够更直接地定义SDL的动态语义，具有易于维护和可读性好等特点。     

网格数据传输的服务质量研究

通过引入服务等级协商协议(SLA)和并行传输机制,对网格中数据并行传输的体系结构进行了研究,针对具有服务质量保证的数据服务的传输进行分析,以及对数据传输质量控制进行性能评测,得出本系统的数据传输质量控制过程可以正确、有效地控制文件传输带宽以及整个传输过程.在分片传输过程中,多个服务器相互协作,共同实现文件传输.     

Preparation of MCM-41-supported chiral Salen Mn （Ⅲ） catalysts and their catalytic properties in the asymmetric epoxidation of olefins

A secondary amino-modified mesoporous molecular sieve MCM-41 was obtained by reaction of bis(3-(triethoxysilyl)propyl)amine with MCM-41. The chiral Salen-Mn (III) complex was anchored onto the modified MCM-41 by a multi-step grafting method and two heterogenized catalysts with different Mn contents were obtained. The catalysts were characterized by XRD, N2 adsorption, ICP, FT-IR and DR UV-Vis. Their catalysis on asymmetric epoxidation of several olefins was studied with NaClO and m-CPBA as oxidants respectively. It was found that both the activity and enantioselectivity of the cata- lysts decreased after the homogeneous catalyst was heterogenized. The reasons resulting in the de- crease of catalytic performance were discussed.     

电动汽车用电池SOC定义与检测方法

为建立电动汽车电池管理系统的需要 ,探求铅酸电池荷电状态 (SOC)的实时测量和估计方法 ,分析了当前 SOC定义在变电流放电情况下出现不适应的原因 ,现有各种荷电状态检测方法的特点和存在的问题。在此基础上 ,对 SOC定义进行了修正 ,提出了“标定荷电状态”和“动态荷电状态”的概念 ,使之能很好地适应电动汽车用电池在变电流状态下的实时荷电状态估计。基于修正 SOC定义的电池荷电状态检测方法和计算模型具有简便、实用和可靠性     

Vp of muscovite-biotite gneiss up to 950℃ at 400 MPa: Constraints on the origin of abnormal seismic layers in continental crust

Here we present experimental results of compressional wave velocity (Vp) of muscovite-biotite gneiss from Higher Himalayan Crystallines (HHC) at the temperature up to 950℃ and the pressure of 0.1―400 MPa. At 400 MPa, when the temperature is lower than 600℃, Vp decreases linearly with increasing temperature at the rate of (Vp/T)p -4.43×10-4 km/s ℃. In the temperature range of 600―800℃, Vp drops significantly and the signal is degraded gradually due to the dehydration of muscovite and α-quartz softening. When the temperature rises from 800℃ to 875℃, Vp increases and the signals become clear again as a result of the temperature going through the β-quartz range. The experiments indicate that the duration has great influence on the experimental results when temperature is above the dehydration point of biotite. During the first 30 h at 950℃, the Vp decreases substantially from 5.9 to 5.4 km/s and the signal amplitude is attenuated by more than 80%. After the 30-h transition, the Vp and the amplitude of ultrasonic wave signals become steady. The decrease of Vp and attenuation of the signals at 950℃ are associated with the breakdown reactions of biotite. The experiments suggest that the breakdown of muscovite and/or quartz softening can contribute to the low seismic wave velocity in thickened quartz-rich felsic-crust such as what is beneath southern Tibet. Additionally, α-β quartz transition generates a measurable high seismic velocity zone, which provides a possibility of precisely constraining the temperature in the upper-middle continental crust. Our study also demonstrates that duration is a key factor to obtain credible experimental results.     

Spontaneous formation of tubular microstructure vesicle from PTA／SDS system

Spontaneous vesicle formation and transformation of vesicle to tubular microstructure have been observed in aqueous mixtures of tri-(n-dodecyl-dimethyl-hydroxy-propylammonium chloride) phosphate (PTA) and sodium dedecyl sulfate (SDS), which is supported by negative-staining transmission electron microscopy (TEM), scan electron microscopy (SEM) and dynamic light scattering (DLS). Spherical vesicles aggregate to tubular microstructures with their diameters and lengths respectively of about 100 and 7000 nm in the freshly prepared sample at the molar ratio of PTA/SDS=3/7 and a total surfactant concentration of 0.01 mol/L. The diameter, the length and the quantity of tubular structures all increase with the increase of the total concentration of surfactant and the sample aging.     

全井眼地层微成像仪测井图像失真的恢复技术

基于全井眼地层微成像仪(FMI)的仪器结构和测量原理,对井下仪器的运动状态进行分析,推导出描述仪器运动状态的微分方程,在此基础上对仪器的遇卡-解卡事件进行识别,然后利用卡尔曼滤波方法对井下仪器的运动状态作出最佳估计,得到原始采样数据的真实深度,最后利用Akima插值算法对极板数据进行重采样并生成成像图像.结果表明:经过校正后生成的FMI图像有效地消除了由于井下仪器的非匀速运动引起的拉伸、压缩、扭曲等图像失真现象,恢复了地层的原貌.该技术不仅适用于FMI成像测井,同样适用于其他进行了加速度测量的成像测井,具有推广应用价值.     

Surface Dissociation Properties of Short Chain Carboxyl Mercaptan Self-Assembled Monolayers by Impedance Titration

Impedance titration was used to determine the surface dissociation characteristics of short-chain carboxyl mercaptan self-assembled monolayers （SAMs）. Based on the change of the cyclic voltammetric peak current and the charge-transfer resistance, which was related to pH value of the solution, the surface pKa of mercaptoacetic acid（MA）, 3-mercaptopropionic acid（MPA） and ω-mercaptohexanic acid（MHA） self-assembled membranes, with ionic strength being 0.1 mol/L, were determined to be 5.20, 4.80, 7.40, respectively. In addition, factors such as time needed for assembling, structure of monolayers and ionic strength, which effected the surface pKa, were studied as well. Such surface pKa shifts were sufficiently explained by interactions between interfacial molecules and hydrophobicity.     

基于动态建模思想的物料清单柔性化定义

为满足各个企业及其部门对物料清单(BOM)形式与意义的不同需求,以动态建模思想为基础,提出了BOM的柔性化定义.分析了BOM的数据组成及其特点,指出了产品数据与BOM之间的二维相关性,利用面向对象的方法建立了BOM柔性化定义对象模型,通过5个元类描述适合企业具体情况的实体对象类和关系对象类,从而动态建立具体的BOM对象模型.该方法已应用于工艺信息管理系统的设计中.结果表明该方法能很好满足企业对BOM定义的个性化定制和动态更改的需求.