High resolution linkage disequilibrium and haplotype maps for the genes in the centromeric region of chromosome 15 in Tibetans and comparisons with Han population

WITH THE SUCCESSFUL COMPLETION OF THE HUMAN GE- NOME PROJECT, ONE OF THE SCIENTIFIC MILESTONES, GENETIC VARIATIONS AND THEIR FUNCTIONAL IMPLICATIONS, HAVE BE- COME ONE OF THE FOCUSES IN GENOME RESEARCH. IT HAS BEEN KNOWN THAT GENETIC VARIATIONS, TOGETHER WITH ENVI- RONMENT, ARE RESPONSIBLE FOR THE DIFFERENCES IN COMPLEX TRAITS IN INDIVIDUALS: PHYSICAL CHARACTERISTICS, DISE…     

Study of CH4 and CO oxidation from electrochemical method

THE TWO FACTORS USED FOR EVALUATING THE QUALITY OF CATALYST ARE I) THE AMOUNT OF ACTIVE SITE PARTICIPATING IN THE REACTION, I.E. THE AREA OF REDOX PEAKS; AND II) THE REGENERATION FREQUENCY OF ACTIVE SITE IN THE REACTION, I.E. THE MATCH OF REDOX POTENTIALS. THE CONTRIBUTION OF EACH FACTOR HAS ITS OWN STRONG POINT IN DIFFERENT REACTIONS, WHILE CYCLIC VOLTAMMETRY (CV) IS A SUITA…     

Post-transcriptional gene silencing signal could move rapidly and bidirectionally in grafted Arabidopsis thaliana

RNA-MEDIATED GENE SILENCING IS A UNIVERSAL GENE- REGULATION SYSTEM FUNDAMENTAL IN BIOLOGICAL PROCESSESAND PHENOMENA OF THE DOUBLE-STRANDED RNA (DSRNA) TRIGGERED SEQUENCE-SPECIFIC MRNA DEGRADATION[1―3]. RNA SILENCING OCCURS IN A BROAD RANGE OF EUKARYOTIC …     

Mapping of the rice （Oryza sativa L.） thermo-sensitive genic male sterile gene tms5 with EST and SSR markers

VELOPING“TWO-LINE”HYBRID RICE.THE POLLEN FERTILITY OF TGMS IS REGULATED BY THE TEMPERATURE OF ENVIRONMENT.THE POLLENS OF TGMS LINES ARE STERILE WHEN THE ENVI-RONMENT TEMPERATURE IS ABOVE A CRITICAL POINT,BUT FERTILE BELOW THIS POINT.SO FAR,A NUMBER OF T…     

Theoretical analysis of the backdraft phenomena induced by liquid fuel

1 INTRODUCTION THE BACKDRAFT PHENOMENON IS DEFINED AS AN EXPLO- SION CAUSED BY THE INRUSH OF FRESH AIR FROM ANY SOURCE OR CAUSE (SUDDEN OPEN OF DOOR OR CRASH OF GLAZING), INTO A BURNING BUILDING, WHERE COMBUSTION HAS BEEN TAKING PLACE IN A SHORTAGE OF AIR…     

Barrier layer in the northeastern South China Sea and its formation mechanism

A VERTICALLY UNIFORM LAYER OF TEMPERATURE(ISOTHERMAL LAYER),SALINITY(ISOHALINE LAYER)AND DENSITY(MIXED LAYER)IS USUALLY FORMED IN THE UPPER OCEAN DUE TO THE WIND STIRRING.UNDERNEATH IS THE LAYER WITH RELATIVELY STRONG VERTICAL GRADIENT AS THE THERMOCLINE,…     

Regulation of microRNA on plant development and viral infection

THE FIRST MIRNA WAS IDENTIFIED IN C. ELEGANS AS EARLY AS IN 1993; THE IMPORTANCE OF MIRNAS, HOWEVER, IS RECOGNIZED ONLY RECENTLY AFTER THE DISCOVERY OF MIRNAS EXISTING UNIVERSALLY IN EUKARYOTIC ORGANISMS. THE SECOND MIRNA WAS IDENTIFIED IN 2000[1]. SINCE …     

Asymmetry between the upper and lower visual fields： An event-related potential study

ASYMMETRIES OF HUMAN VISUAL INFORMATION PROCESS- ING ACROSS VISUAL FIELDS HAVE BEEN AN INTERESTING ISSUE IN COGNITIVE PSYCHOLOGY AND NEUROSCIENCE. IN ADDITION TO INVESTIGATIONS OF THE ASYMMETRIES BETWEEN THE LEFT AND RIGHT VISUAL FIELDS THAT ARE BELIEVED …     

Influences of marine floor hydrothermal activity on organic matter abundance in marine carbonate rocks——A case study of middle-upper Proterozoic in the northern part of North China

FOR A LONG TIME, RESEARCHERS HAVE BEEN REGARDING THE BIOLOGICAL PRODUCTIVITY, THE SEDIMENTATION RATE, THE DEPOSITION ENVIRONMENT, AND THE PRESERVATION CONDITION OF THE DEPOSITIONAL ORGANIC MATTER AS THE CONTROLLING FACTORS OF ORGANIC MATTER ABUNDANCE IN T…     

Synthesis of nanoporous silica with interior composite cells with synthetic block copolypeptide as template

IN NATURE,PROTEIN AND POLYSACCHARIDE ARE INVOLVED IN THE FORMATION OF A REMARKABLE DIVERSITY OF EXQUISITE NANOSTRUCTURED SILICATES IN BIOLOGICAL SYSTEMS SUCH AS DIATOMS AND SPONGES.THE SILAFFIN[1]AND SILICATEIN[2]ISOLATED FROM MARINE DIATOMS AND SPONGES C…     

Structural and expressional analysis of a cDNA that expresses predominantly in rice stamens

The development of stamen is an important stage during the sexual reproduction in higher plants. Studying the genes that predominantly or even specifically expressed in stamens will aid in undestanding the molecular mechanism of stamen development. The structure of a rice CHS-like gene (D5) which predominantly expressed in anthers has been analysised, showing high structural similarity with two other anther-specific genes (A1 and BA42) fromBrassica napus. Northern analysis also reveals that the three genes share a similar expression pattern and that the expression of D5 may relate to the early development of pollens. It is concluded that D5, A1 and BA42 represent a group of diverged CHS-like genes which are related to stamen development.     

栽培黄芩生长过程中CHS和UBGAT表达及黄芩苷积累动态研究

分别采用荧光实时定量PCR和高效液相色谱法分析测定了4月到10月栽培黄芩生长过程中CHS(查尔酮合酶)及UBGAT(UDP-葡萄糖醛酸:黄芩素7-O-葡萄糖醛酸基转移酶)表达水平和黄芩苷含量的动态变化规律.结果表明CHS及UBGAT表达水平均在6月份和8月份达到高峰,而黄芩苷含量的最高峰出现在8月份.研究结果提示在黄芩栽培过程中,6月份和8月份是黄酮类合成和积累的关键时期,因此在这两个月加强田间管理对于提高黄芩药材的质量至关重要.     

Molecular evolution of the exon 2 of CHS genes and the possibility of its application to plant phylogenetic analysis

The exon 2 of chalcone synthase (CHS) gene is relatively conserved during evolution. In this study, three exon 2 fragments from two species in gymnosperm (Cycas panzhihuaensis, Ginkgo biloba) and seven from four species in angiosperm (Magnolia denudata, Salix babylonica, Nymphaea tetragona, Camellia japonica) have been amplified by PCR from genomic DNA and sequenced. Together with other 73 sequences ofCHS collected from EMBL database and literature, these sequences, which embrace 19 families of gymnosperm and angiosperm, have been analyzed for their phylogenetic relations by parsimony method. The result indicated that sequences from the same systematic family usually grouped together except those from Theaceae, Magnoliaceae and Nymphaeaceae. The relative rate test revealed the rate heterogeneity of CHS genes among the families. For the nucleotide substitution the sequences from Asteraceae and Solanaceae evolve faster than those from the other families analyzed while the sequences from Poaceae, Asteraceae and Solanaceae evolve faster for the nonsynonymous substitution. These results suggest that the duplication and extinction events of CHS genes are different among systematic families, therefore it seems impractical to look for orthologous sequences from CHS genes to study plant phylogeny at the family level andlor above. However, it is possible to do so below the family level.     

AtNCED3及AtAAO3基因启动子功能分析及其驱动CHS基因表达

NCED3及AAO3系ABA信号积累的关键基因,其转录调控研究是细胞ABA信号精细调节及植物抗逆分子机制阐明的关键.通过NCED3及AAO3启动子结构与功能的分析,建立了NCED3及AAO3启动子驱动CHS基因表达受干旱诱导致烟草花色变化的体系.     

NaCl和CHS对棉花幼苗的渗透胁迫效应

在短期条件下,用ＣＨＳ（浓缩Ｈｏａｇｌａｎｄ培养液）、ＣＨＳ并在根际加压、ＮａＣｌ溶液、ＮａＣｌ溶液并在根际加压处理棉花幼苗,ＣＨＳ与ＮａＣｌ溶液等渗（渗适势均为－０．４８ＭＰａ）,根据加压＋０．４８ＭＰａ。结果表明ＮａＣｌ和ＣＨＳ的渗透胁迫效应可抑制棉花幼苗的生长,还可减少叶蒸腾率、植物体含水量并降低木质部汁液渗透势。     

Modification of a viral satellite DNA-based gene silencing vector and its application to leaf or flower color change in Petunia hybrida

Gene silencing conserved in plants and animals is mediated by mechanisms that involve sequence- specific RNA degradation[1,2]. Gene silencing has been proven to play an important role in the study of gene function. Recently, a procedure known as virus ind…     

查尔酮合酶基因在植物防御反应中的调控作用

论述了查尔酮合酶基因(chs)在植物防御反应中与病原物的相互关系,探讨了与查尔酮合酶因诱导表达相关的顺式作用元件和反式作用因子及其DNA-蛋白质间的相互作用,阐明其在植物抗病性防御反应中的重要作用。     

我国社区卫生服务信息化的现状与对策

社区卫生服务信息化建设是我国医疗卫生改革的突破口。分析了我国社区卫生服务信息化发展的现状，指出目前我国社区卫生服务信息化建设存在的问题，并提出相应的对策。     

基于CHS软件的飞机线束设计应用

飞机电气系统的线束设计主基于AutoCAD的二维图纸,和excel表格来完成。这需耗费大量的人力时间来完成从原理图到线束分叉图的整个设计过程。而且由于二维图纸没有校验措施,错误较难避免,且大量表格维护更是费时费力。为此将美国Mentor Graphics公司的CHS（Capital Harness System）专业线束设计软件引入飞机电气系统线束设计的二维图纸设计过程,并根据飞机设计求进行二次开发,使所设计的图纸可进行电气校验,建立电气元器件库整合进二维图纸的数据库,大大的提高了设计的效率,同时也保证了设计的可靠性和可维护性。     

不同花色品种蝴蝶兰花色素苷含量分析及相关基因表达研究

分析红色、黄色、粉紫色和白色蝴蝶兰品种不同开放度的花和叶花色素苷生物合成途径7个结构基因PAL、4CL、CHS、DFR、F3H、F3'5H和ANS基因和3个调节基因的表达.结果显示:10个基因的表达因不同花色、不同发育阶段而异.这10个基因在叶中的表达量相对花较低.黄金甲中PAL、4CL、CHS、F3H、F3'5H基因的表达量均较高,PAL、4CL、CHS和F3'5H在开花期达到峰值,粉色花空港红鹰花DFR、CHS、F3H、F3'5H表达量较高.白色花空港枫叶和黄色花富乐夕阳花中CHS、DFR、F3H、F3'5H和ANS表达量较低.CHS在6个品种花的不同阶段皆有表达.F3H基因在6个品种中表达量较低,与花色素合成不存在直接相关.调节基因b HLH 6个品种各发育阶段的表达量均较高,MYB表达量较低,这些结果表明,蝴蝶兰花色素苷积累是CHS、F3'5H、DFR和ANS等关键结构基因共同表达的结果,编码转录因子b HLH基因的表达可能在花色形成中作用较大.