长角血蜱蜕皮激素含量与卵巢微粒体Na^＋—K^＋—ATP酶…

为阐明蜕皮激素在长角血蜱生殖中的作用机制，测定了雌蜱不同生殖时期体内（果和血淋 蜕皮激素含量和卵巢微粒体Ｎａ＾＋－Ｋ＾＋－ＡＴＰ酶活力的变化，结果表明，产卵前，蜕 皮甾类含量和酶活力变化不明显，产卵时，则达到最高；产卵后，蜕皮甾类含量和酶活力又下降，外源蜕皮激素（２０－羟基蜕皮素酮）处理饱血３ｄ雌蜱有激活酶活力作用，但不表现剂理依赖关系。     

长角血蜱蜕皮激素分泌部分的研究

饱血后１，３，５天的长角血蜱，其卵巢的合神经节的蜕皮激素含量在组织培养前后均没有明显变化；体壁在组织培养后，以酮（Ｅ）含量在组织培养前分别增加了１４．３和５．２倍；脂肪体单独进行组织培养时，Ｅ含量均略有下降，２０－羟基蜕皮酮（２０－Ｅ）均略有上升；脂肪体连同体壁一起培养时２０－Ｅ比组织培养前分别增加了１．７，２．３和４．７倍，显示了体壁是Ｅ的合成分泌部位，脂肪体是Ｅ转化为２０－Ｅ的部位。     

荧光染料离子缔合作用在荧光法测定微量DNA中的应用

建立了高灵敏荧光分光光度测定ＤＮＡ 和ＲＮＡ 的新方法． 方法的线性范围分别为０－０４ ～１－２０μｇ／ｍ Ｌ（ 鲑鱼精子ＳＭＤＮＡ 和小牛胸腺ＣＴＤＮＡ） , ０－１０ ～１－２０μｇ／ｍＬ（ 酵母ＲＮＡ） , 检测限分别为１７ｎｇ／ｍＬ（ＳＭＤＮＡ） , ２４ｎｇ／ｍＬ（ＣＴＤＮＡ） , ９８ｎｇ／ｍＬ（ＲＮＡ） ． 将方法用于实际样品金黄色葡萄球菌中ＤＮＡ 含量的测定, 结果满意．     

长角血蜱蜕皮激素含量与卵巢微粒体Na~+-K~+-ATP酶活力的关系(英文)

为阐明蜕皮激素在长角血蜱生殖中的作用机制，测定了雌蜱不同生殖时期体内（卵巢和血淋巴）蜕皮激素含量和卵巢微粒体Na＋－K＋－ATP酶活力的变化．结果表明，产卵前，蜕皮甾类含量和酶活力变化不明显；产卵时，则达到最高；产卵后，蜕皮甾类含量和酶活力又下降．外源燃皮激素（20－羟基蜕皮酮）处理饱血3d雌蜱有激活酶活力作用，但不表现剂量依赖关系．     

陕北红枣叶片中几种生物物质的含量分析

分析了五个陕北红枣品种（梨枣、骏枣、狗头枣、大木枣、圪土达枣）中糖类（含还原性糖和淀粉）、粗脂肪、蛋白质、核酸（含ＤＮＡ和ＲＮＡ）的含量，并对各品种之间的各物质含量进行了比较分析，发现其间存在着较为显著的差异。研究表明，在６月上旬，陕北红枣近顶端新鲜叶片中各成分的含量为：还原性糖０．８０－２．５０ｍｇ／ｇ，淀粉１０．００－３３．２０ｍｇ／ｇ，粗脂肪８．４－１３．９ｍｇ／ｇ，蛋白质２５．４９－２８．２７ｍｇ／ｇ，ＤＮＡ０．５８－１．３５ｍｇ／ｇ，ＲＮＡ４．２０－８．９６ｍｇ／ｇ。此工作为红枣的进一步研究打下了基础。     

小鼠4.5S RNA逆转座子cDNA的克隆及其表达载体的构建

利用ＲＴ－ＰＣＲ方法,从小鼠肝脏组织总ＲＮＡ中扩增出４．５Ｓ ＲＮＡ的ｃＤＮＡ．此ｃＤＮＡ被克隆到ｐＧＥＭ３Ｚｆ（＋）质粒,酶切鉴定并测序。然后将该序列插入以虫荧光素酶基因作了报导基因的表达载体ｐＳＶｌｕｃ２０的ＰｖｕⅡ位点,构建;了４．５Ｓ ＲＮＡ逆转座子的表达载体ｐＳＶｌｕｃ２０－４．５Ｓ,并对４．５Ｓ ＲＮＡ逆转座子的生物学功能进行了研究。     

蜕皮激素在长角血蜱生殖过程及胚胎发育过程中的应用

用反相高效液相色谱法分离测定了长角血蜱提取物中的活性物质a－蜕皮激素和β－蜕皮激素的含量。结果表明：长角血蜱成虫血淋巴中的蜕皮激素在产卵前出现了一高峰；卵巢中的蜕皮激素则在饱血后第三天出现高峰；另外，还发现长角血蜱血淋巴、卵巢中的蜕皮激素含量在吸血交配前出现了一个小峰。在卵的发育过程中，蜕皮激素含量一直升高，持续到幼虫孵出。     

灭幼脲对云斑天牛的不育作用及核酸含量的影响

用灭幼脲处理的补充营养寄主植物饲养云斑天牛Ｂａｔｏｃｅｒａｈｏｒｓｆｉｅｌｄｉ（Ｈｏｐｅ）成虫，对子代卵的孵化有明显抑制作用，生化分析表明：灭幼脲可以促进雌虫卵巢组织ＤＮＡ，ＲＮＡ及一日龄卵ＤＮＡ的合成；对一日龄卵内ＲＮＡ含量影响不显著，对发育末期胚胎的ＤＮＡ，ＲＮＡ有明显抑制作用。结合灭幼脲在世代间垂直传递途径，讨论了灭幼脲对云斑天牛成虫不育作用的机理。     

水分胁迫下不同抗旱性小麦品种核酸代谢的差异

抗旱和不抗旱小麦品种在渗透势为－０．０２５ＭＰａ和－０．０５ＭＰａ ＰＥＧ溶液中根际胁迫３天和５天，叶片的ＤＮＡ、ＲＮＡ含量均呈下降趋势，但抗旱品种７３－１１下降幅度比敏感品种晋２１４８小。ＲＮＡ电泳结果表明：２３ｓＲＮＡ、１８ｓＲＮＡ、１６ｓＲＮＡ以及小分子量ＲＮＡ的含量均下降，２个品种的下降差异不明显，但在峰形上有区别。ＤＮＡ电泳结果表明，ＤＮＡ各组分含量在水分胁迫下也表现为下降趋势，且２个品     

氯化钠介质中核酸对铈（Ⅲ）的荧光猝灭特性及其分布应用

在ｐＨ〈６．２的ＮａＣｌ介质中，Ｃｅ的荧光为核酸所猝灭。２０℃时小牛胸腺ＤＮＡ，鱼精子ＤＮＡ和酵母ＲＮＡ对Ｃｅ的荧光猝灭在０－６．０μｇ／ｍｌ的浓度范围内符合方程ｌｎ（Ｆ０／ｆ）＝ｋｃ＋ｂ，在３．０－８．０μｇ／ｍｌ范围内却符合主程Ｆ０／Ｆ＝ｋｃ＋ｂ。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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Brief Introduction to “Journal of Jilin University( Science Edition) ”

<正>"Journal of Jilin University(Science Edition)"is a comprehensive academic journal in the fields of science sponsored by Jilin University and administrated by the Ministry of Education of the Peoples Republic of China.The journal started publication in 1955.The original name at starting publication was"Journal of Natural Science of Northeast People University",which was changed into"Acta Scientiarum Naturalium Universitatis Jilinensis"in 1958 owing to the name change of the university.The present journal name has