My library的大学馆个性化定制服务浅析

大学图书馆通过My library提供个性化定制服务是图书馆个性化服务的重要组成部分,是网络时代"以人为本"、"以用户为中心"理念的现实体现;通过个性化服务,可以在某种程度上提高大学馆的服务水准和服务效益.     

基于Human library的高校图书馆阅读服务创新

概述了Human library活动的产生与发展过程,分析了Human library阅读服务的创新,探讨了高校图书馆开展Human library活动的优势,提出了Human library在高校阅读推广中的策略。     

图书馆新服务——Living library

本文介绍了近几年国内外新兴起的图书馆服务Living library,阐述了其国内外的发展状况,简单总结其在国外、国内发展中的特点,并分析了其特性,探讨了Living library在国内高校图书馆的开展。     

Research of the Kernel Operator Library Based on Cryptographic Algorithm

The variety of encryption mechanism and algorithms which were conventionally used have some limitations. The kernel operator library based on Cryptographic algorithm is put forward. Owing to the impenetrability of algorithm, the data transfer system with the cryptographic algorithm library has many remarkable advantages in algorithm rebuilding and optimization, easily adding and deleting algorithm, and improving the security power over the traditional algorithm. The user can choose any one in all algorithms with the method against any attack because the cryptographic algorithm library is extensible.     

从Google的新算法看图书馆的变数

探析了Google Library计划对图书馆未来发展和馆员职业取向的深远影响,阐述了Google library对中国图书馆数字化建设的启迪。     

My library的大学馆个性化定制服务浅析

大学图书馆通过My library提供个性化定制服务是图书馆个性化服务的重要组成部分,是网络时代“以人为本“、“以用户为中心“理念的现实体现;通过个性化服务,可以在某种程度上提高大学馆的服务水准和服务效益。     

浅谈Web2.0时代的library2.0

本文针对图书馆面临的挑战与机遇,谈到了web2.0在图书馆中的应用,即library2.0,并对web2.0技术在图书馆中的运用做了几点思考。     

Intrusion Detection Method for Program Vulnerability via Library Calls

Library function call sequence is the direct reflection of a program＇s behavior. The relationship between program vulnerability and library calls is analyzed, and an intrusion detection method via library calls is proposed, in which the short sequences of library call are used as signature profile. In this intrusion detection method, library interposition is used to hook library calls, and with the discussion of the features of the library call sequence in detail, an algorithm based on information-theory is applied to determine the appropriate length of the library call sequence. Experiments show good performance of our method against intrusions caused by the popular program vulnerabilities.     

基于Human Library的高校图书馆阅读疗法服务创新

本文介绍了Human Library的概况、特色及优势,分析了human library新模式的阅读疗法在高校图书馆的可行性,得出Human Library是高校图书馆阅读疗法新药方的结论。     

CDTF-Library’s Expansion to an E-Library

CDTF-Library is a library in Chinese-German Technical Faculty of Qingdao University of Science and Technology in Qingdao.The library was established in the spring of 2004.The CDTF-Library is very small in scale.For our limited budget,the best solution to solve the problems is to expand the library to an e-library on the Internet.So I decided to collect German resources from internet at first,and set up a website for the readers as the subject for the practical customer service project design.At the same time I set up a chat room as an advertisement for the e-library.In the chat room played back audio and video materials what the e-library have.     

A five-fold pig bacterial artificial chromosome library: a resource for positional cloning and physical mapping

A pig BAC library was constructed with genomic DNA from a male Erhualian pig. After partial digestion with Hind III or BamH I the fragments obtained were cloned into the pBeloBAC11 vector. The library consists of 184320 clones which stored in 480 pieces 384-well plates (20 plates per superpool). A two-step 4-dimension PCR screening system was established to screen the positive clones. An average insert size of 128 kb was estimated from 105 randomly isolated clones, which indicates that the library is more than five times of genomic coverage. For the demonstration of the probability to pick out any unique genes or DNA markers from the library, 10 single-copy genes were screened out and the positive clones were yielded between 1 and 8 with an average of 3.6. Positive superpools were obtained for 32 microsatellite markers selected from different regions of pig genome. The number of positive superpools for each marker varies from 1 to 9 with an average of 4.78. This BAC library provides an additional resource for pig physical mapping and gene identification.     

Preliminary study on discovery of a novel molecule that interacts with CPP32 using two-hybrid system

Of ICE protease family, CPP32 (apopain or Yama) plays a central role in different apoptotic pathways. To study the molecules regulating CPP32, yeast two_hybrid system was used to identify proteins (peptides) that interact with CPP32. First, the CPP32 gene was cloned into plasmid vector pGBT9. The resulting recombinant plasmid was designated as pGBT9/CPP. The pGBT9 /CPP plasmid was transformed into the yeast strain HF7C, then the leukemia library was introduced. The transformation mixture was plated on medium lacking Trp, Leu and His in the initial screen. Colonies growing on the selection medium were further assayed for β galactosidase activity. Within 42 Trp +Leu +His + colonies only 5 turned blue in the presence of X_Gal. Plasmid DNA from 5 positive yeast colonies was prepared respectively and used to transform HB101 by electroporation. The transformation mixture was plated on medium lacking Leu to be selected for the library plasmid. Finally, only one library plasmid, designated as pY1, was determined to be truly positive by retransformation of pGBT9/CPP and the library plasmid into HF7C. The inserted cDNA of pY1 encodes a peptide of 15 amino acids, suggesting that it may be the domain interacting with CPP32.     

Community structure of β-Proteobacterial ammonia-oxidizing bacteria in prawn farm sediment

To examine the community structure of β-Proteobacterial ammonia-oxidizing bacteria （AOB） in prawn farm sediment, the 16S rRNA gene library was constructed with β-Proteobacterial AOB-specific primers. The library was screened by PCR-restriction fragment length polymorphism （RFLP） analysis and clones with unique RFLP patterns were sequenced. Two groups of β-Proteobacterial AOB, the Nitrosomonas and the Nitrosospira, were detected. The Nitrosomonas occupied an absolute dominant position, accounting for more than 90% of total clones in the clone library, while the Nitrosospira accounting for 5.48%. Nitrosomonas-affiliated clones were grouped into the Nitrosomonas marina and the Nitrosomonas sp. Nm143 clusters, and Nitrosospira-affiliated clones were grouped into the Nitrosospira cluster 1. No other clusters of β-Proteobacterial AOB were found. The results enriched our knowledge of AOB diversity in the prawn farm sediment and provided important foundational data for further functional studies of these microbes in mariculture environments.     

Screening and Insert Size Analysis of Candidate C-Genome BAC Clones from Brassica napus

Thirty-two C-genome specific candidate bacterial artificial chromosome (BAC) clones were successfully screened from the BAC library by four-dimensional PCR method with the primer pairs of 75 simple sequence repeat (SSR) markers located in the nine C-genome linkage groups of Brassica napus. The screened 32 BAC clones have an average insert size of 114.2 kb with a range of 30-190 kb. They are the first set of C-genome BAC clones screened from B. napus genomic BAC library. The average insert size of this set of BAC clones presented that the constructed BAC library had a high quality. This set of BAC clones can be used as markers to identify individual chromosomes of B. napus C-genome.     

Construction and characterization of a bacterial artificial chromosome library of peach

This report briefly describes the construction and characterization of a peach [ prunus persica (L.) Batch] Var. Jingyu bacterial artificial chromosome (BAC) library. The variety Jingyu has many important agronomic characters of stone fruits, and it is a main parent in Chinese peach breeding. After cloning of the high molecular weight peach DNA into pBeloBAC 11, we obtained over 22 000 recombinant clones. The BAC library has an average insert size of 95 kb and represents approximately 7 times peach haploid genome equivalents. After being screened with two randomly amplified polymorphic DNA markers, W4 and P20, which are linked to yellow flesh and nectarine genes of peach respectively, ten positive clones have been detected. This library is very useful for map-based cloning of peach genes and physical mapping of peach genome.     

Evaluation of Cross-Strait Public Library Website

0 IntroductionLibraryis an organization that mainly serves to provide in-formation. The resource,quality,authorities of informa-tion,and individualization of services ,all reveal different com-petitive strategies and positioning of librariesininformationserv-ices . With the rise of the Digital Library, every library con-structs the library Web sites of different style and category.Theinformation supplying service ranges fromthe on-line bibliogra-phy retrieval , to multi-platforminformation d…     

数字时代高校图书馆用户需求分析与微服务研究

随着全球互联网的推广普及,各行各业都发生了翻天覆地的变革。高校图书馆全面进入library2.0数字时代,用户的需求也不断变化,传统意义上的图书馆服务面临着重大的变革。为了顺应数字化潮流,积极拓展新的服务,高校图书馆必须了解用户的需求,利用一切可利用的手段、资源来提供个性化、碎片化、多元化的微服务。     

Construction of Large Human Single—chain Antibody Phage Display Library

A large human naive single chain antibody (scFv) library is constructed from 60 healthy donors via phage display technique.During the period,some methods are employed to optimize the diversity,such as multi donors,different annealing temperature,half-nest PCR.and assembly by two-way fusion PCR.In this study,78 electroporations resulted in 1010 library,diversity of which is assayed by enzyme fingerprint.The efficiency and diversity are all better than other researches.