小鼠附睾精子的显微受精

目的：建立显微受精的方法,并探讨小鼠附睾精子在显微注射进入卵母细胞后的受精能力。方法：用显微注射法把小鼠附睾头和附睾尾精子注入卵母细胞的胞质内或卵周隙进行显微受精。结果：把单个附睾尾精子注入卵细胞质中,培养后１４个存活的卵细胞中,有５个卵裂为２－细胞期胚胎;将单个附睾头精子注入卵母细胞中,有２５个存活,其中９个受精发育为２－细胞期胚胎;将附睾尾精子注入卵周隙进行带下受精,３０个存活的卵细胞中有４个     

Role for sperm in spatial patterning of the early mouse embryo

Despite an apparent lack of determinants that specify cell fate, spatial patterning of the mouse embryo is evident early in development. The axis of the post-implantation egg cylinder can be traced back to organization of the pre-implantation blastocyst. This in turn reflects the organization of the cleavage-stage embryo and the animal-vegetal axis of the zygote. These findings suggest that the cleavage pattern of normal development may be involved in specifying the future embryonic axis; however, how and when this pattern becomes established is unclear. In many animal eggs, the sperm entry position provides a cue for embryonic patterning, but until now no such role has been found in mammals. Here we show that the sperm entry position predicts the plane of initial cleavage of the mouse egg and can define embryonic and abembryonic halves of the future blastocyst. In addition, the cell inheriting the sperm entry position acquires a division advantage and tends to cleave ahead of its sister. As cell identity reflects the timing of the early cleavages, these events together shape the blastocyst whose organization will become translated into axial patterning after implantation. We present a model for axial development that accommodates these findings with the regulative nature of mouse embryos.     

关于亚纯函数的拟Borel例外值与例外函数

该文讨论了亚纯函数的l级精简拟Borel例外值不等式的一种改进,同时,还将一个拟Bosel例外值的重要不等式推广到了拟Borel例外函数的情形.     

Java异常处理

目的通过Java异常处理的特点和机制,处理程序中出现的错误。方法根据Java异常捕获、异常抛出的要求,分析异常处理的方法。结果结合实际,提出了如何有效地抛出异常和捕获异常的实用策略。结论异常处理提高了程序的安全性和健壮性。     

关于哥德巴赫数的例外集--Dirichlet函数零点的基本引理

设ｑ０,ｑ１,ｑ２是任意正整数,ｘ是一充分大的正实数,０〈δ〈１／３是一固定的这数,ε是充分小的正实数,Ｑ＝Ｘ＾δ,满足「ｑ０,ｑ１」＾ε（ｑ０,ｑ１）和「ｑ０,ｑ２」≤Ｑ（ｑ０,ｑ２）本文给出「ｑ１,ｑ２」≤Ｑ＾２ε（ｑ１,ｑ２）。     

In vitro production of functional sperm in cultured neonatal mouse testes

Spermatogenesis is one of the most complex and longest processes of sequential cell proliferation and differentiation in the body, taking more than a month from spermatogonial stem cells, through meiosis, to sperm formation. The whole process, therefore, has never been reproduced in vitro in mammals, nor in any other species with a very few exceptions in some particular types of fish. Here we show that neonatal mouse testes which contain only gonocytes or primitive spermatogonia as germ cells can produce spermatids and sperm in vitro with serum-free culture media. Spermatogenesis was maintained over 2?months in tissue fragments positioned at the gas-liquid interphase. The obtained spermatids and sperm resulted in healthy and reproductively competent offspring through microinsemination. In addition, neonatal testis tissues were cryopreserved and, after thawing, showed complete spermatogenesis in vitro. Our organ culture method could be applicable through further refinements to a variety of mammalian species, which will serve as a platform for future clinical application as well as mechanistic understanding of spermatogenesis.     

Morrey空间中的例外集和Hausdorff测度（英文）

研究了Morrey空间中的例外集问题,证明了零容度例外集的可去性.另外,也得到了零容度集合的Hausdorff维度,并利用Hausdorff测度刻画集合的容度.     

异常环境下应用软件失效分析

分析应用软件在运行中与环境之间的关系,以及在异常环境下软件容易失效的原因,并提出软件开发过程中针对异常环境应该给出适当的反应或处理.     

制造单元设计中瓶颈问题的处理

近几年来，不少文章提出了在制造单元设计中评价机床利用率的不同方法，多数论文是假设按带有单元间输送作为预定参数的网络模型来处理的，从这一观点出发，为消除所谓瓶颈因素的其他经济的方法可以重复配置机床成将某些工序扩散到单元外加工，本文分析了在处理瓶颈问题时单元间输送的作用，集中分析了它的非独立性性质，因而较大地简化了解决这一问题方法的复杂性，此外，文中还讨论了在处理瓶颈问题时将某些工序扩散到单元外加工的     

谈附加疑问句的特殊情况

在英语中，附加疑问句通常有很多特殊情况，例如疑问部分与陈述部分的主语和谓语不一致，肯定与否定形式有例外情况，等等，应引起我们的充分注意。     

Effects of ubiquitin-proteasome pathway on mouse sperm capacitation, acrosome reaction and in vitro fertilization

Chlortetracycline (CTC) fluorescence patterns were used to study changes in the patterns B and AR of mouse sperm after incubation with reagents that would block the UPP. They were the monoclonal antibody against ubiquitinated proteins—UCP1; the polyclonal antibody against ubiquitin—anti-Ub, and a special inhibitor against proteasome—ALLN. Furthermore, we treated the capacitated sperm or the eggs with these reagents separately and tested whether the normal in vitro fertilization was blocked or not. Results illustrate that UCP1, anti-Ub, and ALLN have little effects on sperm capacitation and acrosome reaction, but they do inhibit fusion of mouse sperm with eggs, which suggests that UPP play an important role in mouse in vitro fertilization.     

Effects of ubiquitin-protea-some pathway on mouse sperm capacitation, acrosome reaction and in vitro fertilization

Chlortetracycline (CTC) fluorescence patterns were used to study changes in the patterns B and AR of mouse sperm after incubation with reagents that would block the UPP. They were the monoclonal antibody against ubiquitinated proteins-UCP1; the polyclonal antibody against ubiquitin anti-Ub, and a special inhibitor against proteasome ALLN. Furthermore, we treated the capacitated sperm or the eggs with these reagents separately and tested whether the normal in vitro fertilization was blocked or not. Results illustrate that UCP1, anti-Ub, and ALLN have little effects on sperm capacitation and acrosome reaction,but they do inhibit fusion of mouse sperm with eggs, which suggests that UPP play an important role in mouse in vitro fertilization.``     

多数据库中例外模式和高选票模式的挖掘

文章在分析前人算法不足的基础上,提出了一种改进的挖掘多数据库中例外模式和高选票模式的算法,算法分析结果表明改进的算法能更加准确有效地在多数据库中挖掘出潜在的例外模式和高选票模式.