Hydrogen ion binding of bacterial cell wall

Riassunto Il cell-wall diStaphylococcus aureus è capace di legare cationi. Poichè la capacità legante ioni dipende dal pH del mezzo, si è studiata la capacità legante idrogenioni di cell-wall liofilizzato isolato daS. aureus. Titolazioni al di sotto di pH 9.0 hanno dimostrato la presenza di 3 siti leganti con pK rispettivamente di 7,15, 4,70 e 3,19 e capacità massima legante di 2, 28 e 232 -equiv. /g (peso secco cell-wall). Viene discussa la natura chimica dei gruppi dissociabili.     

Calcium transport by red blood cell membranes from young and adult cattle

Summary It is shown using inside-out membrane vesicles that cattle red cells extrude calcium by means of a calmodulin sensitive Ca-pump whose activity is high in calves and extremely low in adult cows. The deline is not due to loss of calmodulin susceptibility nor to a drop in Ca-affinity.     

Calcium transport by red blood cell membranes from young and adult cattle

It is shown using inside-out membrane vesicles that cattle red cells extrude calcium by means of a calmodulin sensitive Ca-pump whose activity is high in calves and extremely low in adult cows. The decline is not due to loss of calmodulin susceptibility nor to a drop in Ca-affinity.     

Calcium binding in chemically skinned fibers of rat myocardium during force development

Summary The amout of bound calcium and force were measured in chemically skinned rat myocardial fibers during isometric contraction at different concentrations of free Ca²⁺. The data obtained suggest that calcium binding by cardiac myofibrils is cooperative and probably depends on mechanical tension.The author expresses his gratitude to Dr V. I. Kapelko for valuable discussion of the experimental results.     

Calcium binding in chemically skinned fibers of rat myocardium during force development

The amount of bound calcium and force were measured in chemically skinned rat myocardial fibers during isometric contraction at different concentrations of free Ca2+. The data obtained suggest that calcium binding by cardiac myofibrils is cooperative and probably depends on mechanical tension.     

Effect of erythropoietin on red cell differentiation binding of erythropoietin to DNA

Zusammenfassung Die Fähigkeit der DNS verschiedener Rattengewebe, Erythropoietin zu binden, wurde durch Differentialzentrifugation im Sucrosegradient untersucht. Es ergab sich, dass DNS von Knochenmark, Leber, Milz und Thymus Erythropoietin binden, DNS von Hirn und Nieren Erythropoitein hingegen nicht zu binden vermögen.     

The oxygen-linked hydrogen ion binding (the Haldane coefficient) of bovine hemoglobin.

The Haldane coefficient (the amount of the oxygen-linked hydrogen ion binding of hemoglobin) was determined in bovine erythrolysate (Hb concentration equals 13.5 mM) by means of the differential titration method with varying PCO2 from 0 to 74 mm Hg and pH from 6.0 to 8.5 at 37 degrees C. The maximum value of the coefficient was found to be 0.49 mM per mM Hb at PCO2 equals 0 and pH 7.20. With increasing of PCO2, the coefficient became smaller in all ranges of pH studied. The coefficient under the conditions of pH 7.20 and PCO2 equals 45 mm Hg that are normally prevailing in the interior of bovine erythrocytes was 0.31.     

On the binding of gelatine to modified serum proteins through formaldehyde in an experimental blood volume expander

Zusammenfassung Beim Formolieren und Erhitzen einer Mischung von Rinderserum und teilweise abgebauter Gelatine wird ein Teil der Gelatine kovaliert an die Moleküle des bei diesem Prozess modifizierten Serums gebunden. Die Trennung der noch freien von der an die Serumproteine gebundenen Gelatine konnte durch Gelfiltration mit Sephadex G 200 und durch fraktionierte Fällung mit Trichloressigsäure erreicht werden.     

Red blood cell electrolytes in essential hypertension

Zusammenfassung Mit empfindlicher Methode wurde der Na-, K- und H₂O-Gehalt der Erythrozyten von Hochdruckpatienten und normalen Personen untersucht und kein Elektrolytunterschied zwischen normalen und hypertensiven Versuchspersonen gefunden.     

Studies of the blood ofAscidia ceratodes. Total blood cell counts,differential blood cell counts,hematocrit values,seasonal variations,and fluorescent characteristics of blood cells

Summary Seasonal, and animal size and weight variations of the blood cells of the vanadium-containing ascidian,Ascidia ceratodes, were determined. The fluorescent properties of various cell types were ascertained, and discussed in terms of cell development, phylogenic position of the species, and chemicals in the cells.This investigation was supported by a grant from the Research Corporation and one of us (W.R.B.) received partial support from various University of California administrated fellowships. The current address of W.R.B. is Chevron Research Products, Richmond, CA. The use of the facilities at the Bodega Marine Laboratory, University of California are acknowledged.     

Lead binding to human haemoglobin

Zusammenfassung Die Verbindung von Blei mit menschlichem Hämoglobin wurde in vitro mittels Pb-203 untersucht, wobei man die Anzahl der zugänglichen Verbindungsstellen am Molekül des Hämoglobins und der Verbindungskonstante für den Blei-Hämoglobinkomplex bestimmte. Die Verbindung war weder von der Anwesenheit der -Ketten noch von freien Sulphydrylgruppen abhängig. Mittels EDTA konnte Blei von Hämolysaten, nicht aber aus intektan Erythrozyten entfernt werden.     

Mercury binding to human haemoglobin

Zusammenfassung Mittels²⁰³Hg als Hg-Chlorid wurden die freien SH-Gruppen des Hämoglobins in einem stromafreien Hämolysat titriert und festgestellt, dass die durchschnittliche Zahl freier, reaktiver SH-Gruppen pro Molekül 2,5 betrug.

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Lead-203 was kindly supplied by the M. R. C. Cyclotron Unit, Hammersmith, London. D. B. is a Wellcome Senior Research Fellow in Clinical Science.