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1.
Summary Studies on the sarcomere length-force relation in Ca++-activated vertebrate muscle myofibrils indicate that the maximum isometric force can be generated even at sarcomere lengths around 1 m, indicating that the ascending limb of the sarcomere length-force relation is virtually absent.We thank Miss S. Gomi for her technical assistance. This work was supported by the grant (No. 587028) form the Ministry of Education, Science and Culture of Japan. 相似文献
2.
Summary Cinematographic recordings of Ca-activated contraction in glycerinated bundles of myofibrils of the horseshoe crab striated muscle indicated that the A-band width did not change during rapid sarcomere shortening from 11 to 4 m, while it increased by about 10% in nearly isometric conditions.We wish to thank T. Taniguchi, M. Kusama and M. Goto for their excellent technical assistance in taking cinematographic recordings. 相似文献
3.
Summary Isolated cardiac cells from bullforg atrial tissue can be readily prepared by digestion of intact fragments of atrial tissue with trypsin and collagenase. These isolated cells have dimensions of about 5 m in width and range in length from 300 m to over 500 m. Such isolated cells may prove useful for the investigation of contractile activity of cardiac muscle at the single cell level and at the sarcomere level within the single cell.This investigation was supported by U.S. Public Health Service, National Institutes of Health Grant No. HL 12426 and a Kansas Heart Association Grant-in-Aid.We wish to thank Dr.J. Sommers of Duke University Medical Center for doing electron microscopy of the isolated cells to confirm the single cell nature of the preparations. 相似文献
4.
Antidiuretic effects of oxytocin in the Brattleboro rat 总被引:1,自引:0,他引:1
J. Lyness A. G. Robinson M. N. Sheridan D. M. Gash 《Cellular and molecular life sciences : CMLS》1985,41(11):1444-1446
Summary The antidiuretic activity of oxytocin (OT) was measured in Brattleboro rats with congenital diabetes insipidus. A dose dependent antidiuretic response was found in animals receiving chronic infusions of 0.1 g/h, 1.0 g/h, and 5 g/h of OT. OT infused at the rate of 5 g/h over a 7-day period completely reversed the symptoms of diabetes insipidus. The results support the concept that OT serves as a weak agonist of vasopressin at the level of the kidney and at pharmacological levels exhibits antidiuretic activity. 相似文献
5.
Summary In the rabbit jejunum, the elevation of temperature within the range of 25–37°C diminished the sensitivity to noradrenaline (NA) for both the relaxation and amplitude inhibition. The relaxation by NA was mainly mediated via adrenergic -receptors at 25, 30 or 37°C. The amplitude inhibition was mediated via -receptors at 37°C, and both - and -receptors at 30 or 25°C. 相似文献
6.
Hypothalamic histamine modulates adaptive behavior of rats at high environmental temperature 总被引:1,自引:0,他引:1
K. Fujimoto T. Sakata K. Ookuma M. Kurokawa A. Yamatodani H. Wada 《Cellular and molecular life sciences : CMLS》1990,46(3):283-285
Summary Histamine content in the rat hypothalamus was lower at 4°C and higher at 31°C compared to that at 21°C. Pretreatment with -fluoromethylhistidine, a suicide inhibitor of histidine decarboxylase, attenuated both the increased level of hypothalamic histamine and rat adaptive behavior at 31°C. Increase of histamine content in the hypothalamus appears to be an important factor contributing to rat adaptive behavior to high environmental temperature. 相似文献
7.
I. A. Macdonald 《Cellular and molecular life sciences : CMLS》1978,34(4):426-427
Summary Tritium distribution on randomly labelled taurocholate (TC) was estimated at 28%, 4%, 1% and <0.5% on the hydrogens opposite the 3-, 7- and 12-OH groups and taurine moiety respectively. Anomalously,C. perfringens 3-hydroxysteroid dehydrogenase (3-HSDH) catalyzed tritium loss of 36% on formation of 7-, 12-dihydroxy-3-keto-5-cholanoate, implying additional losses of tritium at other sites by this enzyme.This work is supported by the National Cancer Institute. 相似文献
8.
T. Nagatsu T. Kato Y. Numata K. Ikuta M. Sano I. Nagatsu T. Takeuchi M. Matsuzaki H. Umezawa 《Cellular and molecular life sciences : CMLS》1977,33(5):581-583
Summary Serum dopamine--hydroxylase activity in spontaneously hypertensive rats and Wistar-Kyoto rats had a positive correlation with dopamine--hydroxylase and tyrosine hydroxylase activities in mesenteric vessels, vas deferens, and adrenal glands at 14–16 weeks of age, a negative correlation with dopamine--hydroxylase activity in locus coeruleus at 3 weeks and 14–16 weeks of age, and a positive correlation with tyrosine hydroxylase activity only at 3 weeks of age, but not at 14–16 weeks of age. 相似文献
9.
In the present study we show, for the first time, that phenylephrine (PHE), an -adrenergic receptor agonist, potentiates the effect of isoproterenol (ISO), a -adrenergic agonist, in activating pineal type II5-deiodinase (5-D) activity. The potentiating effect of PHE was observed only at doses of ISO which induce submaximal activation of the enzyme. However, at doses which lead to maximal activation of the enzyme, PHE was ineffective. The results suggest that not only -, but also -adrenergic receptors, are involved in the sympathetic noradrenergic regulation of pineal 5-D activity in the adult rat. 相似文献
10.
Summary The total cerebellar proteins RNA and DNA contents from DLT4- and LT3-treated rats was studied at 6 and 35 days of age. The effect of injections of 5 g/j of DLT4 is comparable to that of 25 g of LT3 at birth, followed by 0.5 g every 2 days. On the other hand, injection of 0.5 g of LT3 every 2 days does not induce any significant modification of the total DNA contents in the cerebellum. 相似文献
11.
Hotspots of homologous recombination 总被引:9,自引:0,他引:9
G. R. Smith 《Cellular and molecular life sciences : CMLS》1994,50(3):234-241
Homologous recombination occurs at higher than average frequency at and near hotspots. Hotspots are special nucleotide sequences recognized by proteins that promote, directly or indirectly, a rate limiting step of recombination. This review focuses on two well-studied examples, the Chi sites of the bacteriumEscherichia coli and the M26 site of the fission yeastSchizosaccharomyces pombe. Chi, 5 G-C-T-G-G-T-G-G 3, is recognized by the RecBCD enzyme, which nicks the DNA near Chi and produces a 3-ended single-stranded DNA tail; this tail is a potent substrate for homologous pairing by RecA and single-stranded DNA binding proteins. M26, 5 A-T-G-A-C-G-T 3, is recognized by a heterodimeric protein and stimulates, by an as-yet-unknown mechanism, meiotic recombination at and near theade6 gene. Additional hotspots in bacteria, fungi, and mammals enhance recombination directly or indirectly via a variety of mechanisms. Although hotspots are widespread among organisms, the biological role of their localized enhancement of recombination remains a matter of speculation. 相似文献
12.
Pujol E Rodríguez-Cuenca S Frontera M Justo R Lladó I Kraemer FB Gianotti M Roca P 《Cellular and molecular life sciences : CMLS》2003,60(9):1982-1989
Gender- and site-related differences in the lipolytic capacity, at the different steps of the adrenergic pathway, in gonadal and inguinal white adipose tissue (WAT), were assessed by studying 2A-adrenergic receptor (AR), 3-AR and hormone-sensitive lipase (HSL) protein levels, and by determining the lipolytic response to different agents. Gonadal WAT showed a lower 2A/3-AR ratio, a greater lipolytic capacity in response to AR agonists, and higher HSL activity and protein levels than inguinal WAT. In female rats, we found greater 2A-AR protein levels and 2A/3-AR ratio compared to their male counterparts, but, on the other hand, a higher lipolytic response to -AR agonists and a greater lipolytic capacity at the postreceptor level, including a more activated HSL protein. Thus, the lipolytic capacity was clearly higher in gonadal than in inguinal WAT, at the different steps of the adrenergic pathway studied. Moreover, in both tissues, females showed a greater inhibition of lipolysis via 2-AR, which was counteracted by the higher lipolytic capacity at the postreceptor level.Received 1 April 2003; received after revision 11 June 2003; accepted 23 June 2003 相似文献
13.
Summary Ouabagenin (I) has been degraded stepwise to the known 3-methoxy-17-carbomethoxy-1,3,5:10-estratriene (XII). Thus the presence of a normal steroid skeleton and the -configuration of the butenolidering at C-17 is proven. The 14-configuration of the hydroxyl at C-14 was also proven. As a result of these and earlier findings, structure I can be definitely assigned to ouabagenin.
Glykoside und Aglykone, 178. Mitteilung. 相似文献
Glykoside und Aglykone, 178. Mitteilung. 相似文献
14.
Summary The conversion of cholesterol into 19-nor-5-cholestan-3-ol by the spongeAxinella polypoides involves a partial loss (40%) of the 3-hydrogen atom; moreover administration to the sponge of [4-14C]cholesterol tritiated at C-4 and C-7 showed that the 4- and 7-hydrogen atoms are retained in this conversion. A competitive uptake experiment, [4-14C]cholesterol vs. [7-3H2]5-cholestanol, showed that the sponge utilized exclusively cholesterol for the production of 19-nor-5-cholestan-3-ol.Part of this work has been presented at the Nato Conference on Marine Natural Products (Jersey, Great Britain, October, 1976).-This contribution is part of the Programma finalizzato Oceanografia e Fondi marini-sottoprogetto Risorse biologiche C.N.R. Italy. 相似文献
15.
P. Gandhi 《Cellular and molecular life sciences : CMLS》1978,34(1):15-15
Summary Incubation of 5,5-7,7-tetramethoxy-8,8-biflavone withAspergillus niger results in the formation of 4,4-dihydroxy-5,5-7,7-tetramethoxy-8,8-biflavan.Part of this work was done at Allahabad University, Chemical Laboratories. 相似文献
16.
W. Emser R. Dechoux M. Weiland A. Wirz-Justice 《Cellular and molecular life sciences : CMLS》1993,49(8):686-687
In a double-blind placebo crossover study of 13 healthy volunteers, the pineal hormone melatonin (10 mg) was given at 4 pm, and the electroretinogram measured under conditions of dark and light adaptation. A significant diminution of b-wave amplitude was found under both photopic (=5.4 V, p<0.05) and scotopic conditions (=7.4 V, p<0.01). These data indicate that melatonin may transduce the dark signal at the level of the retina as well as the pineal. Acute administration of melatonin decreases sensitivity to light. 相似文献
17.
Summary Uptake ofd-glucose anomers by isolated rat retina was studied. After 3 min incubation at 37°C in the presence of or anomer (750 g/ml), a significantly greater uptake (1.32 mg/g wet tissue) of -anomer was observed compared with that of -d-glucose (1.11 mg/g wet tissue). This result and other data suggest that the carrier ford-glucose transport in the retina prefers the -anomer stereospecifically.Acknowledgment. The authors are grateful to Mr S. Suzuki for his technical assistance. 相似文献
18.
F. Rollo W. Asci S. Antonini I. Marota M. Ubaldi 《Cellular and molecular life sciences : CMLS》1994,50(6):576-584
The paper reports on the molecular analysis of samples of approximately 5,300-year-old grass found at the alpine archaeological site where the so-called Tyrolean Iceman was discovered. The grass comes from a cloak made of long grass blades and/or the stuffing of the snow footwear worn by the Iceman. The results show that while the largest fraction of the DNA extractable from the grass is of foreign origin, a much smaller part belongs to the original genetic material of the grass itself, and can be used as a valuable taxonomic clue to the plant species utilized by neolithic men to manufacture their equipment. On the other hand, the foreign DNA, or at least a portion of it, comes from microorganisms-mainly filamentous fungi and unicellular algae-which seem to have been associated with the grass since the time the grass was harvested. 相似文献
19.
Summary The synthesis of 17-hydroxy-17-hydroxymethyl-4-androsten-3-one fromReichsteins compound S is described. Transformation of 3,3-ethylenedioxy-17-hydroxy-17-hydroxymethyl-5-androsten into 17-methyl-isotestosterone demonstrates the configuration of the substituents at C-17. 相似文献
20.
A. Locker 《Cellular and molecular life sciences : CMLS》1959,15(3):100-101
Summary (1) The influence of temperature on the dependence of tissue-respiration upon body-size is examined in those tissues of cold- and warm-blooded animals, which are similar with respect to their general enzymatic activity, namely the skin of frogs (intraspecific comparison) and the liver of mice, rats, and guinea pigs (interspecific comparison).(2) In both tissues the respiration decreases more, with increasing body-size, at higher temperature than at lower temperature.(3) This effect of temperature on size-dependent activities is possibly rather common. When poikilothermic and homoiothermic animals are compared, these relationships must be considered. 相似文献