影响复迭代分形图的若干因素分析

提出影响复迭代变换Z=Zα C对应分形图的一系列因素,并利用计算机图示实验和数据分析方法对这些影响因素进行了分析。     

乳酸乳球菌电转化条件的研究

以质粒pTRKH2为材料,对乳酸乳球菌MG1614进行电转化条件的研究.实验结果表明,在电转化过程中,电场强度、电阻大小、细胞生长状态和电击后菌株复苏时间均对转化效率有明显影响,得到了乳酸乳球菌的最适电转化条件为:对数生长中期的细胞,经1%甘氨酸处理,在10kVcm电场强度、400Ω电阻下电转化,转化后细胞在SGM17培养液中培养2h后涂布选择性培养基,转化率(转化子个数与DNA质量之比)可达1.2×103个μg,比优化前的转化效率提高近100倍.     

用芬太尼抑制全麻气管插管引起机体的应激反应

全身麻醉气管插管时刺激厌喉、气管,机体对此可产生过激的应激反应,可能导致对机体的损伤,采用芬太尼可抑制机体的应激反应［１］。临床多采用血压（ＢＰ）、心率（ＨＲ）及病人的反应来作为应激反应的指标,由于它们易受到多种因素的影响,评价应激反应不够客观。近１...     

Action mechanisms of a new erythrocyte-derived depressing factor

To investigate the action mechanisms of a new erythrocyte-derived depressing factor (EDDF), the focus is placed on the effect of EDDF on both cytosolic and nuclear free calcium (Ca²⁺) transportation in vascular smooth muscle cell (VSMC), as well as the apoptosis and cell cycle of VSMC of rats. EDDF has been extracted from human erythrocytes. The changes of Ca²⁺ levels in cytoplasm ([Ca²⁺]_i) and nucleus ([Ca²⁺]_n) have been observed using a laser scanning confocal microscope together with fluo-3/AM as a calcium indicator. Flow cytometric technique was used to study the effect of EDDF on cell cycle and apoptosis of VSMC. [Ca²⁺], and [Ca²⁺]_n were significantly decreased through several different pathways: ( i ) it reduced the Ca²⁺ influx by blocking L-type voltage-dependent calcium channel (L-VDC) and R-type voltage-dependent calcium channel (R-VDC); (ii) it inhibited the Ca²⁺ release from inositol 1, 4, 5-trisphosphate (IP₃) sensitive calcium store; and (iii) activated Ca²⁺-ATPase of sarcoplasmic reticulum (SR) and promoted the transportation of Ca²⁺ from cytoplasm to SR. However, EDDF seemed to have little inhibitory effect on the Ca²⁺ release from ryonodine sensitive calcium pool. It was also found that EDDF (10⁻⁴ g/mL) significantly decreased the proportion of S phase of human umbilical vein (HUV) and inhibited the proliferation of VSMC induced by angiotensin II (Angll, 10⁻⁵ mol/L). The apopotosis did not occur when VSMC was cultured under normal condition. While VSMC apoptosis was induced by Angll (10⁻⁵ mol/L) and EDDF (10⁻⁴ g/mL) seemed to have little effect on it. The inhibitory effect of EDDF on the elevation of [Ca²⁺]_i and [Ca²⁺]_n of VSMC might play an essential role in its action mechanisms and the ways it affects the Ca²⁺ handling of VSMC demonstrate that EDDF was different from other endogenous blood pressure regulators and some known antihypertensive drugs. EDDF could inhibit the proliferation of VSMC, which indicated that it might be beneficial to the prevention and treatment of hypertension and arteriosclerosis.     

影响农杆菌介导的辣椒基因转化因素的研究

试验设置农杆菌类型、辣椒基因型、外植体、vir基因活化培养基、方法5个因素，研究了利用根癌农杆菌介导法辣椒遗传转化的效果。以获得Km抗性芽外植体频率为指标，发现农杆菌菌株类型和辣椒基因型对转化效率都有较大影响，直接影响着转化效率。采用预培养2d的子叶外植体，用农杆菌侵染5～7min，可获得最高的转化效率。     

页岩储层SRV影响因素分析

为了计算页岩储层压裂改造体积,建立了与施工参数相关联的储层改造体积SRV(Stimulated Reservoir Volume)计算模型。应用该模型研究了弹性模量、泊松比、地应力差、储层厚度等因素对储层改造体积的影响,分析得知泊松比和水平主应力差存在临界值,分别为0.36 MPa和6 MPa,超过临界值后难于形成缝网。通过页岩储层实例分析得出储层改造体积随着压裂排量的增加而增大,施工排量存在一个最佳值10 m3/min;储层改造体积随着液量的增加而增大,增幅先增后减;增大施工压力可增大储层改造体积,但增幅不明显。该模型为页岩储层体积压裂施工参数优选和改造体积预测提供参考。     

小波变换在大学生学业影响因素分析中的应用研究

本文将小波变换应用于大学生学业成绩分析。通过不同班级学业成绩进行问卷调查,运用DB4小波对该数据进行小波变换,从而得到小波细节信号。同时,对该细节信号求方差,对得到的各组细节信号和方差数据进行比较,对不同班级出现的学业差异问题原因进行分析和研判。     

致密油藏直井体积压裂储层改造体积的影响因素

基于三叠系长7致密储层地质特征建立直井典型缝网模型,利用数值模拟方法对体积压裂与常规压裂的开发效果进行对比,模拟不同缝网形态、裂缝间距及导流能力对体积压裂储层改造体积(VSR)的影响。结果表明:体积压裂改造方式能够改善油藏的渗流环境,增加储层动用程度,大幅度提高单井产能;储层改造体积越大,压后产量越高;相同改造体积下,开发效果与井筒连通的有效裂缝体积密切相关;裂缝间距及主、次裂缝导流能力对储层改造体积的影响较大。     

Observation of stimulated emission by direct three-photon excitation

Multiphoton processes, predicted theoretically in 1931, were for a long time considered to be mainly of academic interest. This view changed when it was shown that a two-photon absorption process could, because of a quadratic dependence of excitation on intensity, produce a spatially confined excitation useful for three-dimensional data storage and imaging. Two-photon absorption has received considerable attention recently because of the development of highly efficient two-photon-sensitive materials, leading to numerous technological applications. These successes have created interest in exploring applications based on three-photon excitations. For a three-photon process, a longer excitation wavelength such as those common in optical communications can be used. Also, the cubic dependence of the three-photon process on the input light intensity provides a stronger spatial confinement, so that a higher contrast in imaging can be obtained. Here we report the observation of a highly directional and up-converted stimulated emission as an amplified spontaneous emission, produced in an organic chromophore solution by a strong simultaneous three-photon absorption at 1.3 microm. This achievement suggests opportunities for a three-photon process in frequency-upconversion lasing, short-pulse optical communications, and the emerging field of biophotonics.     

Communication between osteoblasts stimulated by electromagnetic fields

Pulsed electromagnetic field can affect the proliferation of osteoblasts, but the mechanism is obscure yet. The communication between osteoblasts, isolated from calvaria bone of newborn SD rats and stimulated with the rectangular electromagnetic field of 15 Hz and 4 mT, was studied. Our results showed that the osteoblasts radiated a kind of light after they were stimulated with the electromagnetic field and it is the light that promotes the proliferation of un-stimulated osteoblasts.