GroE heat-shock proteins promote assembly of foreign prokaryotic ribulose bisphosphate carboxylase oligomers in Escherichia coli

Assembly of foreign prokaryotic ribulose bisphosphate carboxylases (Rubiscos) in Escherichia coli requires both heat-shock proteins groEL and groES. GroEL is related to a chloroplast protein implicated in Rubisco assembly. Bacteria and chloroplasts therefore have a conserved mechanism that uses auxiliary proteins to assist in the assembly of Rubisco.     

锌试剂-蛋白质体系的共振光散射光谱研究

 在pH 3.0的B-R缓冲溶液中,锌试剂能与蛋白质结合形成复合物.此结合反应能显著加强锌试剂的瑞利光散射信号.详细研究了此结合反应的最佳反应条件,并以此反应为基础,利用共振瑞利散射光技术,建立了一个测定蛋白质的新方法.该方法对牛血清白蛋白(BSA)、人血清白蛋白(HSA)以及免疫球蛋白测定的线性范围分别为0.25~12.5,0.10~15.0μg/mL和0.10~12.5μg/mL,检出限均小于0.05μg/mL,且大量的常见金属离子、氨基酸等共存物质不干扰测定.方法具有很高的灵敏度、很好的选择性及重现性.用于血清样品中蛋白质的测定,结果满意.     

药物与蛋白质相互作用的荧光光谱法研究概述

结合1972年以来的内容、研究方法和进展.     

Finding finer functions for partially characterized proteins by protein-protein interaction networks

Based on high-throughput data, numerous algorithms have been designed to find functions of novel proteins. However, the effectiveness of such algorithms is currently limited by some fundamental factors, including (1) the low a-priori probability of novel proteins participating in a detailed function; (2) the huge false data present in high-throughput datasets; (3) the incomplete data coverage of functional classes; (4) the abundant but heterogeneous negative samples for training the algorithms; and (5) the lack of detailed functional knowledge for training algorithms. Here, for partially characterized proteins, we suggest an approach to finding their finer functions based on protein interaction sub-networks or gene expression patterns, defined in function-specific subspaces. The proposed approach can lessen the above-mentioned problems by properly defining the prediction range and functionally filtering the noisy data, and thus can efficiently find proteins’ novel functions. For thousands of yeast and human proteins partially characterized, it is able to reliably find their finer functions (e.g., the translational functions) with more than 90% precision. The predicted finer functions are highly valuable both for guiding the follow-up wet-lab validation and for providing the necessary data for training algorithms to learn other proteins.     

氟喹诺酮类药物与蛋白质相互作用的研究

运用荧光淬灭机理研究了氟喹诺酮类药物洛美沙星，氧氟沙星，衣诺沙星与3种蛋白质(牛血清蛋白，免疫球蛋白，胰蛋白酶)之间的相互作用，计算了两者之间的结合常数和结合位点数，根据实验当中所得的热力学参数确定了它们之间的作用类型，氟喹诺酮类药物的含量与其荧光强度呈现良好的线性关系，在此实验条件下测定了胶囊及人工合成样中洛美沙星和衣诺沙星的含量，其浓度范围分别为0．018～0．878和0．030～3．171μg／mL。     

第一类抗冻蛋白与冰晶相互作用能的简单计算

第一类抗冻蛋白分子上的苏氨酸或缬氨酸对其在冰晶表面的吸附起主要作用,在此观点的基础上,认为抗冻蛋白分子在冰晶表面的吸附依赖于这些氨基酸上的甲基与冰晶的van der Waals相互作用,从理论上计算了第一类抗冻蛋白分子与冰晶的相互作用能,给出了几种第一类抗冻蛋白分子与冰晶的相互作用能.计算结果与实验结果符合较好.     

Polarized synchronous light scattering characterization of the interaction of proteins with sodium dodecyl sulfonate

In acid buffer solution, proteins with positive charge can react with anion surfactant and result in a great enhancement of synchronous light scattering (SLS) signals. In this contribution, the correlative experiment was made to compare the interaction of human serum albumin (HSA) and immunoglobulin G (IgG) with sodium dodecyl sulfonate (SDS). Based on the measurements of the polarization light scattering signals, a new method of scattering polarization was constituted to distinguish these two interaction systems with molecular weight difference (HSA 66 kDa; IgG 150 kDa). The results were con- sistent with the data measured by dynamic light scattering (DLS) technique.     

多声子对弱耦合体磁极化子性质的影响

研究了磁场中多原子极性晶体中多声子相互作用对弱耦合体极化子基态能量和有效质量的影响．采用么正变换、线性组合算符和拉格朗日乘子法。计算了多声子相互作用对磁极化子基态能量和有效质量的附加贡献．结果表明，基态能量的附加贡献为电子-不同支LO声子-磁场间耦合的附加能量以及电子-不同支LO声子间耦合的附加能量之和，有效质量的附加贡献为电子-不同支LO声子间耦合的附加贡献．     

谈体育教学直观示范的问题

体育直观教学中,位置的选择的必要性.选择恰当的示范位置,学生看清楚所要学的动作.这是示范前首先要考虑的问题;效果的考虑,恰当的示范位置为示范产生效果创造的重要前提,使示范达到预定目的.     

多数据库的数据元素映射移位方法

本文给出一种采用数据映射函数库(向量值函数)实现数据库文件之间数据元素位移的方法,解决了从N个源数据文件自动、高效地生成M个目标数据文件的问题。     

Interactions of pyrogallol red with proteins and the determination of proteins by resonance light-scattering

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用微机演示李萨如图形

提出了用微机演示李萨如图形的简单方法．     

Demonstration by NMR of folding domains in lysozyme

Although there has been much speculation on the pathways of protein folding, only recently have experimental data on the topic been available. The study of proteins under conditions where species intermediate between the fully folded and unfolded states are stable has provided important information, for example about the disulphide intermediates in BPTI, cis/trans proline isomers of RNase A3 and the molten globule state of alpha-lactalbumin. An alternative approach to investigating folding pathways has involved detection and characterization of transient conformers in refolding studies using stopped-flow methods coupled with NMR measurements of hydrogen exchange. The formation of intermediate structures has been detected in the early stages of folding of cytochrome c, RNaseA and barnase. For alpha-lactalbumin, hydrogen exchange kinetics monitored by NMR proved to be crucial for identifying native-like structural features in the stable molten globule state. An analogous partially folded protein stable under equilibrium conditions has not been observed for the structurally homologous protein hen egg-white lysozyme, although there is evidence that a similar but transient state is formed during refolding. Here we describe NMR experiments based on competition between hydrogen exchange and the refolding process which not only support the existence of such a transient species for lysozyme, but enable its structural characteristics to be defined. The results indicate that the two structural domains of lysozyme are distinct folding domains, in that they differ significantly in the extent to which compact, probably native-like, structure is present in the early stages of folding.     

内p-群与外p-群的结构和性质

本文以p-群和内∑-群研究成果为基础,以它们的研究方法为依托,采用反证法、分析法,得到若干成果,丰富了研究内∑-群这一领域的成果．文章首先以可解次单群的结构和性质,来引出文章所讨论的任一真子群为素数方幂阶的有限群的结构和性质,给出来一个有限群满足这一性质的充分必要条件,得到了若干结论,并且指出了任一真子群为素数方幂阶的有限群和有限次单群、CP-群之间的包含关系．最后,进一步拓宽这一性质,引出外p-群的定义,给出了一个外p-群的必要条件．     

Magnification of light from many distant quasars by gravitational lenses

Exceptionally bright quasars with redshifts up to z = 6.28 have recently been discovered. Quasars are thought to be powered by the accretion of gas onto supermassive black holes at the centres of galaxies. Their maximum (Eddington) luminosity depends on the mass of the black hole, and the brighter quasars are inferred to have black holes with masses of more than a few billion solar masses. The existence of such massive black holes poses a challenge to models for the formation of structures in the early Universe, as it requires their formation within one billion years of the Big Bang. Here we show that up to one-third of known quasars with z approximately equal to 6 will have had their observed flux magnified by a factor of ten or more, as a consequence of gravitational lensing by galaxies along the line of sight. The inferred abundance of quasar host galaxies, as well as the luminosity density provided by the quasars, has therefore been substantially overestimated.     

一种多功能治疗仪的设计

为了减轻社会上妇女患有子宫炎、霉菌性及细菌性阴道炎等疾病的痛苦,通过对全自动微电脑触摸屏控制的“妇科清洗辅助治疗仪[”4]深入研究,在此基础上,研制出“多功能(电脑妇科)治疗仪”。     

FENG Guo-dong, et al

Aim To investigate a new method for synthesis of an Immobilized-Metal Affinity Chromatography(IMAC) adsorbent with superparamagnetism(Fe3O4/SiO2-GPTMS-Asp-Co).Method The magnetic microspheres were synthesized in situ modification and the transmission electron microscope,field emission scanning electron microscopy,X-ray diffractometer,energy spectrometer and vibrating sample magnetometer were used to characterize the appearance,particle size distribution,phase composition,chemical constituents and magnetic properties of silica magnetic microspheres(MMS).Results The silica MMS prepared by reverse microemulsions exhibit a superior core-shell structure.The size distribution of the microspheres varies from between 100 nm to 200 nm and the main phase of microspheres is amorphous SiO2 and spinel Fe3O4.The microspheres retain superparamagnetism and can be used as biomaterials.Conclusion The result indicates that the IMAC adsorbent we prepared has outstanding advantages in the separation of the natural products proteins from the crude bacterial lysate,such as simple operation,high selectivity and capacity.     

离子型表面活性剂对血红蛋白谱学性质的影响

利用荧光光谱和吸收光谱技术研究表面活性剂对血红蛋白(Hb)存在形态及结构变化的影响，结果表明，离子表面活性剂的加入使蛋白质分子变性，十二烷基硫酸钠(SDS)使胁荧光增强较十六烷基三甲基溴化铵(CTAB)明显；同步荧光光谱中随波长差(△λ)的增加，胁荧光峰位置发生约10nm的红移，荧光强度也随之发生变化；血红蛋白406nm处Soret带吸收峰加入表面活性剂后降低或升高、红移是由于血红蛋白的构象发生了变化，结论：在实验过程中，胁形成低聚物或单体，结构发生去折叠并伴随血红素基团的裸露，温度及pH值在溶液体系中也会影响胁变性。