几种不同高分子生物材料吸附FG与单克隆抗体M1结合亲合？…

利用鼠抗人血纤维蛋白原单克隆抗体Ｍ１作为血小板替代物,以酶联免疫吸附测定为手段,通过ＦＧ－Ｍ１复合物结合力常数ｋｎ的测定,对几种高聚物材料血液相容性进行了评价。测定了不同材料表面的ＸＰＳ谱,比较了不同组成的材料表面血液相容性的差异。     

SURFACE PINNING AND ITS DETERMINATION BY MAGNETIC RELAXATION

ＳＵＲＦＡＣＥＰＩＮＮＩＮＧＡＮＤＩＴＳＤＥＴＥＲＭＩＮＡＴＩＯＮＢＹＭＡＧＮＥＴＩＣＲＥＬＡＸＡＴＩＯＮＺｅｎｇＺａｏｙａｎｇ１）ＤｉｎｇＳｈｉｙｉｎｇ１）ＹａｏＸｉｘｉａｎ１，２）（１）ＰｈｙｓｉｃｓＤｅｐａｒｔｍｅｎｔａｎｄＳｏｌｉｄＳｔａｔ...     

LEAKY LAMB WAVES IN MULTI-LAYERED FLUID-SATURATED POROUS MEDIA IMMERSED IN LIQUID

ＬＥＡＫＹＬＡＭＢＷＡＶＥＳＩＮＭＵＬＴＩ┐ＬＡＹＥＲＥＤＦＬＵＩＤ┐ＳＡＴＵＲＡＴＥＤＰＯＲＯＵＳＭＥＤＩＡＩＭＭＥＲＳＥＤＩＮＬＩＱＵＩＤＺｈｏｕＹｕｆｅｎｇ１）ＷａｎｇＹａｏｊｕｎ１）ＭａＬｉ２）ＧａｏＴｉａｎｆｕ２）（１）Ｉｎｓｔｉｔｕｔｅ...     

ON THE EXPONENT SET OF PRIMITIVE LOCALLY SEMICOMPLETE DIGRAPHS

ＯＮＴＨＥＥＸＰＯＮＥＮＴＳＥＴＯＦＰＲＩＭＩＴＩＶＥＬＯＣＡＬＬＹＳＥＭＩＣＯＭＰＬＥＴＥＤＩＧＲＡＰＨＳ￥ＺｈａｎｇＫｅｍｉｎｇ１）；ＢｕＹｕｅｈｕａ２）（１）ＤｅｐａｒｔｍｅｎｔｏｆＭａｔｈｃｍａｔｉｅｓ，ＮａｎｊｉｎｇＵｎｉｖｅｒｓｉｔｙ，Ｎ...     

同源四倍体高粱、约翰逊草及杂种F_1的同工酶分析

同源四倍体高粱、约翰逊草及杂种Ｆ＿１的同工酶分析乔亚科（河北农业技术师范学院农学系，昌黎，０６６６００）ＩＳＯＥＮＺＹＭＥＡＮＡＬＹＳＩＳＯＦＡＵＴＯＴＲＡＰＬＯＩＤＳＯＲＧＨＵＭ，Ｓ．ｈａｌｅｐｅｒｓｅ（Ｌ）ＰｅｒｓＡＮＤＴＨＥＩＲＨＹＢＲＩＤＦ＿...     

微波MESFET上变频器

微波ＭＥＳＦＥＴ上变频器张锦卫＊冯立民苗敬峰（东南大学无线电工程系，南京２１００１８）随着微波ＦＥＴ的迅速发展，微波场效应管上变频器的研究引起了人们的兴趣．１９８０年Ａｂｌａｓｓｍｅｉｅｒ等人利用ＧａＡｓＭＥＳＦＥＴ制作了频率较低的上变频器［１］，...     

THE MOTION PROPERTIES OF THE STARS CONFINED TO THE SYMMETRIC AXIS OF AN OSCILLATION KUZMIN DISK

ＴＨＥＭＯＴＩＯＮＰＲＯＰＥＲＴＩＥＳＯＦＴＨＥＳＴＡＲＳＣＯＮＦＩＮＥＤＴＯＴＨＥＳＹＭＭＥＴＲＩＣＡＸＩＳＯＦＡＮＯＳＣＩＬＬＡＴＩＯＮＫＵＺＭＩＮＤＩＳＫＦｕＹａｎｎｉｎｇ１，２）ＳｕｎＹｉｓｕｉ１）（１）ＤｅｐａｒｔｍｅｎｔｏｆＡｓｔｒｏｎ...     

HARMONIC AC SUSCEPTIBILITY FOR TEXTURED YBa_2Cu_3O_7

ＨＡＲＭＯＮＩＣＡＣＳＵＳＣＥＰＴＩＢＩＬＩＴＹＦＯＲＴＥＸＴＵＲＥＤＹＢａ２Ｃｕ３Ｏ７ＧｅＹｏｎｇ１）ＤｉｎｇＳｈｉＹｉｎｇ２）（１）ＤｅｐａｒｔｍｅｎｔｏｆＰｈｙｓｉｃｓ,ＮａｔｉｏｎａｌＬａｂｏｆＳｏｌｉｄＳｔａｔｅＭｉｃｒｏｓｔｒｕｃｔｕｒ...     

TECTONIC DEVELOPMENT OF THE METAMORPHIC CORE COMPLEX OF THE WUGONGSHAN IN THE NORTHERN JIANGXI PROVINCE

ＴＥＣＴＯＮＩＣＤＥＶＥＬＯＰＭＥＮＴＯＦＴＨＥＭＥＴＡＭＯＲＰＨＩＣＣＯＲＥＣＯＭＰＬＥＸＯＦＴＨＥＷＵＧＯＮＧＳＨＡＮＩＮＴＨＥＮＯＲＴＨＥＲＮＪＩＡＮＧＸＩＰＲＯＶＩＮＣＥＳｕｎＹａｎ１）ＳｈｕＬｉａｎｇｓｈｕ１）Ｍ．Ｆａｕｒｅ２）Ｊ．Ｃｈａ...     

MICROSPHERULESINSUZHOUGRANITE：ABLATEDPRODUCTSOFACOMETARYEXPLOSION

ＭＩＣＲＯＳＰＨＥＲＵＬＥＳＩＮＳＵＺＨＯＵＧＲＡＮＩＴＥ：ＡＢＬＡＴＥＤＰＲＯＤＵＣＴＳＯＦＡＣＯＭＥＴＡＲＹＥＸＰＬＯＳＩＯＮＨｕＺｈｏｎｇｗｅｉ１）ＷａｎＹｕｑｉｕ２）ＷａｎｇＥｒｋａｎｇ２）（１）ＤｅｐａｒｔｍｅｎｔｏｆＡｓｔｒｏｎｏｍｙ，...     

Fibrinogen Structural Changes Caused by Glass Surface Adsorption Detected by Solid Surface Fluorescence Spectroscopy

ＦｉｂｒｉｎｏｇｅｎＳｔｒｕｃｔｕｒａｌＣｈａｎｇｅｓＣａｕｓｅｄｂｙＧｌａｓｓＳｕｒｆａｃｅＡｄｓｏｒｐｔｉｏｎＤｅｔｅｃｔｅｄｂｙＳｏｌｉｄＳｕｒｆａｃｅＦｌｕｏｒｅｓｃｅｎｃｅＳｐｅｃｔｒｏｓｃｏｐｙ＊ＧｏｎｇＹａｎｄａｏ（公衍道），ＤｉｎｇＸ...     

农药三唑酮分子印迹聚合物的识别特性研究

采用分子印迹技术合成了对农药三唑酮有高选择性的印迹聚合物,通过Scatchard分析研究了印迹聚合物的结构特性.结果表明,以甲基丙烯酸为功能单体的印迹聚合物,通过氢键作用可以形成两类结合位点.用多点结合模型计算两类结合位点的离解常数分别为K1=7.89×10-4mol/L,K2=3.53×10-3mol/L.底物的选择性结合实验表明,该聚合物对三唑酮呈现高度的选择性及识别能力.为在生物样品中选择富集三唑酮提供了可能性.     

三唑醇分子烙印聚合物的吸附特性

实验采用分子烙印技术合成了对农药三唑醇有特异性作用的分子烙印聚合物(molecularly im-printed polymer,MIP).通过平衡吸附实验,评价了其对三唑醇的亲和力和选择性.与非烙印聚合物相比,分子烙印聚合物对三唑醇表现了较强的亲和力;Scatchard分析表明,以甲基丙烯酸为功能单体,以三唑醇为模板的分子烙印聚合物,通过离子作用和氢键作用可形成两类结合位点.用多点结合模型计算两类不同结合位点的离解常数为Kd1=2.80×10-4mol/L,Kd2=9.71×10-3 mol/L.实验表明,该聚合物对三唑醇有高亲和力和选择性,为在生物样品中选择富集三唑醇提供了可能性.     

低温等离子体在材料表面改性中的应用

概要介绍了目前低温等离子体在材料表面改性方面的研究进展。材料的许多特性,如金属的表面硬度、耐腐蚀、耐磨擦,聚合物的表面浸润性、亲性性、粘附性以及生物功能材料的生物相容性等,决定了材料的应用。低温等离子体并不改变材料的板材特性而仅影响材料的表面特性。对金属如不锈钢等用氮气等离子源离子注入,可以在表面形成Fe2N,Fe3N和Fe4N的铁的氮化物,提高表面的硬度和耐腐蚀性能;氧气、氮气等离子体会在聚合物材料表面形成微针孔结构,改善其浸润性、粘附性;用等离子聚合法在生物材料表面聚合高分子材料,如氧化对二甲苯可以降低血小板的吸附。因此,低温等离子体在材料的表面改性方面有很好的应用前景。     

阿特拉津分子印迹聚合物微球的制备及表征

以阿特拉津（Atrazine）为模板分子,甲基丙烯酸（MAA）为功能单体,乙二醇二甲基丙烯酸酯（EDMA）为交联剂,采用沉淀聚合法制备了粒径约210nm的阿特拉津纳米分子印迹聚合物（MIPs）微球.采用紫外分光光度法确定了模板分子与功能单体的最佳物质的量比为1:4.Scatchard分析表明,分子印迹聚合物纳米微球存在两种不同的结合位点,最大表观吸附量(Q_max)和平衡离解常数（K_d）分别为Q_max1=38.08μg/g,K_d1=0.2489μg/L,Q_max2=310.33μg/g,K_d2=6.6269μg/L.此方法制备的分子印迹聚合物对阿特拉津具有良好的选择性吸附能力.     

活性/可控自由基聚合制备分子印迹纳米膜

通过可逆加成-断裂链转移(RAFT)自由基反应,在球形硅胶表面制备了分子印迹纳米膜.茶碱吸附实验显示,与对照组的杂化球形硅胶相比,分子印迹纳米膜杂化球形硅胶对茶碱具有更高的吸附结合能力.利用RAFT所具有的活性/可控的特点,通过改变实验条件控制分子印迹纳米膜在硅胶表面的生长,可以得到较厚的分子印迹纳米膜以获得较好的吸附量,也可以得到较薄的分子印迹纳米膜以获得较好的吸附效率.     

Size-dependent control of the binding of biotinylated proteins to streptavidin using a polymer shield

Many medical and biotechnological processes rely on controlling and manipulating the molecular-recognition capabilities of proteins. This can be achieved using small molecules capable of competing for protein binding or by changing environmental parameters that affect protein structure and hence binding. An alternative is provided by stimuli-responsive polymers that change reversibly from a water-soluble expanded coil to a water-insoluble collapsed globule upon small changes in temperature, pH or light intensity: when attached to proteins in the vicinity of their binding sites, they reversibly block and release small ligands. Here we show how this approach can be extended to achieve size-selective binding of large, macromolecular ligands. We use the thermally responsive polymer poly(N,N-diethylacrylamide) (PDEAAm), and attach it to the protein streptavidin approximately 20 A from the binding site for biotinylated proteins. Below the lower critical solution temperature of PDEAAm, the polymer is in its extended state and acts as a 'shield' to block the binding of large biotinylated proteins; above this temperature, it collapses and exposes the binding site, thereby allowing binding. We find that the degree of shielding depends on both the size of the biotinylated protein and the size of PDEAAm, suggesting that 'smart' polymer shields could be tailored to achieve a wide range of size-dependent ligand discrimination for use in affinity separations, biosensors and diagnostics technologies.     

抗凝血生物材料研究中的表面分析方法

开发抗凝血生物材料最主要的目的就是要找到一种在与人体血液和组织相接触后不会引起血栓、凝血和炎症等特性的高分子材料,而抗凝血生物材料的以上特性与其表面性质紧密相关.因此,需要对新合成的材料的表面性质进行表征.综述了近年来应用得比较广泛的7种用于抗凝血生物材料表面性质分析的常规方法和1种新兴的核分析方法,并以在研究中的运用为实例,介绍了各种方法的原理、特点以及一些不足,指出只有借助多种分析方法,才能全面了解抗凝血生物材料的表面结构.     

Location of high affinity Ca2+-binding sites within the predicted transmembrane domain of the sarcoplasmic reticulum Ca2+-ATPase

Cation pumps bind and translocate ions with the intermediate formation of a phosphoenzyme. In spite of extensive knowledge of the primary and even secondary structures of several of these cation transport enzymes, however, no high affinity cation binding sites have yet been determined. Here we report the use of oligonucleotide-directed, site-specific mutagenesis to identify the amino acids involved in Ca2+ binding in one of these transport enzymes, the Ca2+-ATPase of sarcoplasmic reticulum. Alteration of Glu 309, Glu 771, Asn 796, Thr 799, Asp 800 or Glu 908, each of which is predicted to lie near the centre of the transmembrane domain in putative transmembrane sequences M4, M5, M6 and M8 resulted in complete loss of Ca2+ transport function and of Ca2+-dependent phosphorylation of the enzyme by ATP. Phosphorylation of each of the mutant enzymes with inorganic phosphate was observed, however, even in the presence of Ca2+, which inhibits phosphorylation in the wild-type enzyme possessing an intact high affinity Ca2+-binding site. These results suggest that at least six polar, oxygen-containing residues lying near the centre of the transmembrane domain provide ligands for one or both of the two high affinity Ca2+ binding sites in the Ca2+-ATPase.