Stimulation of gastric secretion by prostaglandin F2 α in rats

Summary In rats with chronic gastric fistulas, prostaglandin F₂ stimulated the gastric acid secretion in graded doses of 50. 100, 200 and 400 g/kg b. wt, while higher doses above 1 mg/kg b. wt tended to inhibit significantly. The gastric antisecretory effect of prostaglandin E₁ could not be altered or modified by subsequent treatment of prostaglandin F₂ , while the latter alone without any prior treatment of the former, stimulated output of gastric juice, HCl and pepsin without significantly affecting the concentration of these components.Acknowledgement. The gift of the prostaglandin E₁ and prostaglandin F₂ is acknowledged to Dr J.E. Pike, Upjohn, USA. This paper was read at the IXth Annual Conference of the Indian Pharmacological Society held at Benares (1976) and abstracted in the Indian J. Pharmac.9, 73 (1977).     

Antioxidant effects on prostaglandin synthesis in rabbit kidney medulla slices

Summary Sodium diethyldithiocarbamate, 2,6-di-tert-butylphenol and N,N-diphenyl-p-phenylenediamine inhibited the generation of medullary prostaglandin E, but 1,2-dimethoxyethane (OH scavenger) and 2,5-dimethylfuran (¹O₂ scavenger) stimulated basal prostaglandin E production 1.2–1.3-fold. These results suggest that the balance between formation and removal of cellular lipid peroxides sets a peroxide level that can regulate the rate of prostaglandin formation in cells.     

Δ6-Tetrahydrocannabinol-7-oic acid,a urinary Δ6-THC metabolite: Isolation and synthesis

Zusammenfassung 7-Hydroxy- ⁶-tetrahydrocannabinol (7-Hydroxy- ⁶-THC), ein psychotomimetisch aktiver Metabolit des ⁶-THC, wird von Kaninchen in inaktive ⁶-THC-7 Säure metabolisiert. Die Synthese dieser Säure wird beschrieben.

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The work in Jerusalem was supported by the US National Institute of Mental Health and in Stockholm by the Swedish Medical Research Council.     

Isomeric homoallylic cations formed from 19-substituted steroids

Zusammenfassung Die Existenz von zwei verschiedenen isomeren Homoallylkationen in der Umlagerung von Steroid- ⁵-19-Methansulfonsäure-Estern zu 5, 19-cyclo-6-hydroxylierten Steroiden und deren Umlagerung zu ⁵(¹⁰)-B-homo-7-hydroxylierten Steroiden wurde bewiesen, und eine neue Art der Homoallylumlagerung eines ⁵(¹⁰)-B-homo-7-hydroxylierten Steroids zum 5, 6-Methano-9-en und zum 5, 6-Methano-10-ol wurde beobachtet.

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This material was presented at theSymposium on Steroids Made Through Intramolecular Functionalization on the C ₁₈-and C ₁₉-Methyl Groups, at the 149th meeting of the American Chemical Society in Detroit (Michigan), on April 7, 1965.     

Effect of 723-1723-1723-1on ethanol withdrawal in mice

Summary ⁹ (10 mg/kg, i.p.) administered to mice immediately after withdrawal from a 3-day exposure to ethanol vapor was found to intensify withdrawal reactions. No effect was seen when ⁹ was administered chronically during the exposure to ethanol.     

Variations de l'activité des auxines-oxydases dans les racines duLens

Summary 2, 4-dichlorophenol (DCP), 2, 4-dinitrophenol (DNP) and 2, 4-dinitro-o-cresol (DNC) applied at a concentration of 1.10^–7 to 1.10^–3 M, produce a dramatic increase in IAA-oxidase activity in young cells ofLens root. IAA destruction by breis from root tissues was inhibited by Mn⁺⁺ ion at low concentrations of DCP (1·10^– to 1.10^–7 M), but was enhanced by Mn⁺⁺ ion at higher (1.10^–5 to 1.10^–3 M) DCP levels.

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Ce travail a pu être entreprise grâce à l'appui duFonds national suisse pour la Recherche scientifique.     

Control of obesity inA vy /a mice by 5α-androstan-17-one

Summary Treating VY/WfL-A ^vy /a mice with 5-androstan-17-one, a mammalian glucose-6-phosphate dehydrogenase inhibitor, prevented the mice from becoming obese. The weight difference between treated and controlA ^vy /a mice was mainly due to a decreased accumulation of triacylglycerol. The compound did not suppress appetite, had no detectable toxicity and did not affect the lipogenesis rates in the liver and carcass. The weight-controlling effect of 5-androstan-17-one inA ^vy /a mice was reversible upon withdrawal of treatment.The authors wish to thank Mr W.R. Gibson and Drs C.G. Culbertson and P.N. Harris for performing the pathological examinations.     

The partitioning ofΔ 1-tetrahydrocannabinol into erythrocyte membranes in vivo and its effect on membrane fluidity1into erythrocyte membranes in vivo and its effect on membrane fluidity1

Summary ¹-Tetrahydrocannabinol ( ¹-THC) has been quantified directly in erythrocyte membranes from drug-treated mice using gas chromatography/mass spectrometry. Concentrations of approximately 6 ng ¹-THC/mg membrane protein (10^–5 M) were found when effects of the drug on behavior were prevalent. At these concentrations the drug produced a decrease in membrane order as measured by ESR.This work was supported by grants from the Medical Research Council, the Wellcome Trust and the E. P. Abraham Cephalosporin Trust     

Nitrogenase activity in a transfilter culture of rhizobia with a non-leguminous plant callus culture: Transfer of fixed15N2 from bacteria toPortulaca callus

Summary A transfilter culture of the non-leguminous plantPortulaca grandiflora var. JR andRhizobium sp. cowpea 32H1 was established. Using¹⁵N₂-analysis we demonstrated that¹⁵N-containing substances produced by the bacteria passed through the membrane and¹⁵N was enriched in the plant cells.Acknowledgment. The authors wish to express their gratitude to Prof. H. Marschner and Dr G. Hentschel for the determination of the nitrogen-content of our samples by the Dumasmethod and for advice on the optic emission method. Drs J. Burton, Milwaukee, Wisc., and Tjepkema, Oregon State University, are thanked for generous gifts ofRhizobium sp. cowpea 32H1. This work was supported by the DFG.     

Vorgänge bei pH-Änderung und bei Belichtung eines Anthocyanidins

Summary The anthocyanidin investigated here exists below pH 4 as a cationAH ₂ ⁺ (wave length of absorption maximum _max=459 mµ), between pH 5 and 7 in the neutral formAH ( _max=492 mµ) and above pH 8 as an anionA ^– ( _max=537 mµ). At pH 5 the freshly dissolved substance is partially converted into a colourless formBH ( _max=372 mµ) and a chemical equilibrium betweenAH andBH is reached within 1 h. A kinetic study of the process of formation ofBH shows that an intermediate productZ is formed. This process can be reversed by light exposure. It can be concluded from a kinetic investigation by using flash light thatBH is transformed by the absorbing light into a new substanceL ( _max 275 mµ and 225 mµ), andL changes partially intoBH, partially intoZ, which itself is transformed partially intoAH ₂ ⁺ ,AH,A ^–, partially intoBH. The reactionZ AH,AH ₂ ⁺ ,A ^– is proportional to the concentration of protons [H ⁺], the reactionZ BH independent of [H ⁺]. Thus a photochemical production ofAH ₂ ⁺ ,AH,A ^– fromBH is readily obtained in the presence ofH ⁺ and not obtained in the absence ofH ⁺.     

Unterschiedliche Empfindlichkeit verschiedener Angiotensinderivate gegen Abbau durch Plasma oder Nierenhomogenat

Summary -Asp¹-Angiotensin II had a more pronounced action on the blood pressure of nephrectomized rats than the corresponding-compound.-Asp¹-Angiotensins were more slowly destroyed by rat serum, rat kidney homogenate, and human plasma than-compounds, especially-Asp¹-Angiotensin II amide.     

Developmental changes of aldehyde dehydrogenase isozymes in human livers: Mitochondrial ALDH2 isozyme is expressed in fetal livers

Summary Previous reports suggested that the major cytosolic aldehyde dehydrogenase (ALDH₁) was present in fetal and infant livers, but the major mitochondrial isozyme (ALDH₂) was absent or severely diminished. Re-examination by means of starch gel electrophoresis followed by enzyme activity staining, and by means of dot blot immuno-hybridization of liver samples with known genotypes of theALDH ₂ locus, indicated that bothALDH ₁ andALDH ₂ genes are expressed in fetal and infant livers. In addition, ALDH₄ isozyme was also observed. The results imply that a fetus with the usual homozygousALDH ₂ ¹ /ALDH ₂ ¹ genotype, but not one with the atypicalALDH ₂ ¹ /ALDH ₂ ² orALDH ₂ ² /ALDH ₂ ² , is capable of detoxifying acetaldehyde transferred from the mother.     

Single amino acid substitutions insn-glycerol-3-phosphate dehydrogenase allozymes fromDrosophila virilis

Summary The amino acid sequence was compared among the three allelic variants (allozymes) ofsn-glycerol-3-phosphate dehydrogenase inD. virilis, which are detected by one-dimensional electrophoresis. The GPDH^f variant was different from the GPDH^m by only one substitution of 68-lysine for asparagine; GPDH^s differed from GPDH^m by substitution of 127-glycine for arginine. No electrophoretically silent substitutions were found in a total of 352 amino acid residues.     

Transfilter associations of nonleguminous plants with rhizobia: Channelling of N2 fixed by rhizobia into the nitrogen-metabolism ofPortulaca grandiflora callus tissues

Summary In transfilter associations ofPortulaca grandiflora callus tissues withRhizobium sp. cowpea 32H1 the nitrogenium fixed by the bacteria was channelled into the normal pathway of nitrogen metabolism of higher plants. In associations kept in an¹⁵N₂ containing atmosphere 10% of the¹⁵N taken up into the plant cells was incorporated intoPortulaca proteins. The results demonstrate that cells of nonleguminous plants are profiting from the quasi-symbiotic situation.     

The distribution of some enzymes involved in the steroidogenesis of hen's ovary

Riassunto È stata studiata con metodi istochimici la distribuzione della ⁵-3-idrossisteroide deidrogenasi ( ⁵-3-HSDH) e delle 17-idrossisteroide deidrogenasi (17-HSDH), DPN- e TPN-dipendenti, nell'ovario di gallina ovulante. Mentre la ⁵-3-HSDH è presente in tutti i tessuti a secrezione steroide dell'ovario, la 17-HSDH è localizzata solo nelle cellule della granulosa.     

Hashish: Synthesis of Δ9-tetrahydrocannabinol (THC), a metabolite ofΔ 9-THC1

Summary The synthesis of (±)-211-dihydroxy- ⁹-THC, a difunctionalized metabolite of ⁹-THC, is presented.Acknowledgment. This work was carried out with the support of NIDA Grant No. DA 00574. We are grateful to Mr. J. Coe for assistance with some of the experiments.     

Open-field behavior and acquisition of discriminative response control in Δ9-THC tolerant rats

Résumé La tolérance de l'effet du 1- ⁹-tétrahydrocannabinol ( ⁹-THC) a été examinée par une méthode nouvelle. Les résultats obtenus permettent une interprétation de tolérance pharmacologique pour ⁹-THC.

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Numbering system according to IUPAC rules. The drug was generously supplied by Dr.T. Petrzilka, University of Zürich.     

Ein neues Verfahren zur Herstellung von 19-Norsteroiden

Summary The new methods for the production of 19-norsteroids described involve the conversion of 5-halogen-6-hydroxy-steroids into the corresponding 5-halogen-6:19-ethers either with lead tetraacetate or by the hypoiodite reaction^8,10. The 6:19-oxygen bridge is then opened reductively either directly or after oxidation of the 6:19-ethers to lactones or preferably after introduction of a ^4,6-3-oxo grouping. Acylolytic cleavage of the ⁴-3-oxo-6:19-ethers followed by alkaline hydrolysis gives ^4,6-3-oxo-19-hydroxy-steroids. The 19-hydroxycompounds formed are easily converted into 19-norsteroids by known methods.

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Über Steroide, 189. (vorläufige) Mitteilung; 188. Mitt. vgl.J. Kalvoda, J. Schmidlin, G. Anner undA. Wettstein, Exper.18, 398 (1962).     

Mikrobiologische Oxydation von Steroiden durch Cephalosporia

Summary It was found that members of the Cephalosporium species transform steroids under the known conditions of microbiological oxydation. Cephalosporium subverticillatum is able to produce ⁴-testoloactone from progesterone, but, in case of a longer fermentation period, ⁴-androsten-3, 17-dione can be isolated from the broth.     

Über den Phytoöstrogengehalt einiger Pflanzen

Summary The quantity of phyto-estrogens in red clover, hop, peas, and cabbage was estimated by titration on castrated female rats and infantile mice. The level of phytoestrogens was as follows: in hop 1050–2000/kg, in peas 4–6/kg, in cabbage 24/kg, and in red clover 6–9/kg. The author supposes that the sexual disorders described byRosenberger ¹⁴ in cows fed with cabbage, can be explained by the presence of phyto-estrogens in this plant.