沼泽红假单胞菌高产辅酶Q10菌株的诱变选育研究

以沼泽红假单胞菌为出发菌株,通过紫外诱变和罗红霉素抗性筛选,以期获得辅酶Q10高产菌株.试验结果表明,紫外照射时间60-70s时可能达到最大正向突变概率.通过紫外诱变、罗红霉素初筛和发酵复筛,获得一株沼泽红假单胞菌突变株R-1,辅酶Q10产量达到0.021g/L,较出发菌株产量（0.018g/L）提高16％.经过6次传代培养,突变菌株产辅酶Q10产量稳定,可用于进一步研究.     

产脂肪酶菌株Acinetobacter calcoaceticus UN9的诱变选育

本实验菌株醋酸钙不动杆菌(Acinetobacter calcoaceticus)M1-7的最佳紫外诱变时间为60,s.利用紫外诱变后涂布到添加不同脂肪酶底物和分解产物的分离培养基,筛选出具有抗性的正突变株.其中柠檬酸钠抗性突变菌株UN9最高酶活力达15.960,U/mL,比出发菌株提高了130.97%,远远高于琥珀酸钠、正丁酸、正己酸、三丁酸甘油酯等抗性突变菌株.经5次传代后,菌株UN9遗传性能稳定,平均酶活力为15.866,U/mL.     

他克莫司产生菌原生质体制备、再生与诱变

对筑波链霉菌的原生质体制备、再生条件进行了研究,建立了筑波链霉菌的原生质体诱变筛选体系.在此基础上,通过原生质体的紫外诱变处理,筛选得到1株高产菌株,与出发菌株相比,该菌株的平均发酶单位提高51.4%.通过原生质体的紫外诱变来筛选他克莫司高产菌株是一种行之有效的方法.     

氯化锂-紫外-微波复合诱变红霉素高产菌株的研究

以红霉素链霉菌为出发菌株进行诱变、选育高产菌株．探讨了氯化锂、紫外、微波3种诱变方式对红霉素链霉菌的影响,根据后代的存活率和突变率选定合适的诱变条件．结果表明,将这3种诱变方式结合起来可获得较高的正突变率,达到了40．6％．筛选得到诱变菌株W-12,该菌株效价比初始菌株提高了11％,达到了良好的筛选效果．     

灰色链霉菌快中子诱变育种

快中子诱变抗生素菌种,应用于点青霉、红霉素链霉菌等已有报导。链霉素是个已生产了二十多年的老抗生素,生产水平已经很高,而且很不容易再提高。该菌种已经过常用的各种理化因子处理,但至今尚未见到快中子诱变灰色链霉菌的报导。我们用静电加速器产生的快中子辐照灰色链霉菌(Streptomyces griseus),作过十多批处理,经过两年多的菌种选育,证明适当剂量的快中子辐照能诱发灰色链霉菌的菌落形态、色素、生理特性、营养缺陷和产量等的突变,选育到年平均增产26.7％的高产菌株。     

用亮氨酸抗性法选育L-甲硫氨酸高产突变株

用亮氨酸(Leu)抗性为标记筛选L-甲硫氨酸(L-Met)高产突变株．以一株具有M-海因水解酶系统的芽孢杆菌MV0073为出发菌株，经过6轮紫外诱变，筛选出27株抗亮氨酸的L-Met高产突变株．其中突变株M604的L-Met产量是出发菌株的3倍，且培养液中积累的高含量L-Met能在培养后期稳定持续，M604连续传代6次，遗传性状稳定，表明用Leu抗性选育L-Met高产突变株是一种切实可行的方法。     

铜抗性菌株的原生质体诱变选育及其吸附能力检测

将酿酒酵母(saccharomyces cerevisiae)菌株Y1单倍体化，筛选得到单倍体出发菌株SY1，通过紫外线(UV)和亚硝基胍(NTG)复合诱变，获得1株高抗性突变菌株，其Cu^2 抗性水平为8mmol／L，是出发菌株的8倍，其Cu^2 吸附量是出发菌株的4．6倍。50世代培养结果表明，其遗传稳定性在96％以上。     

林可霉素菌种选育及摇瓶培养基的优化

以林可霉素生产菌L10-18林可链霉菌(Streptomyces linloinensis)为出发菌株,采用紫外线(UV)、甲基磺酸乙酯(EMS)进行双重复合诱变处理,以终点产物林可霉素的产率为选择压力进行菌株筛选,结果表明,诱变得到的林可霉素抗性突变株L13-6-3发酵水平比出发菌株提高14.1%.再经过培养基优化,其组成为葡萄糖12 g/L,豆饼粉3.0 g/L,(NH_4)_2S0_40.6 g/L,玉米浆0.2 g/L的条件下,诱变菌株发酵水平再提高5.2%.     

利用紫外线诱变原生质体筛选四环素高产菌株的研究

以金色链霉菌为出发菌株，经溶菌酶作用制备原生质体，然后以紫外线对原生质体进行诱变处理，在再生培养基上再生培养，获得再生突变株，将其中的单菌落以琼脂挖块法进行初筛获得第２０号菌株，其相对效价为１．２８４。     

林可霉素产生菌林可链霉菌高产菌株的选育：Ⅰ.摇瓶初筛

本课题是利用抗生素抗性与产量之间的相关性进行林可菌素产生菌林可链霉菌高产菌株选育。本次实验以湖北制药厂五分厂生产上使用的林可链霉菌８０＾＃菌株为出发菌株，用紫外线与氯化锂进行复合处理，在含林可霉素的平板上筛选出林可霉素抗性有所提高的突变株，再经摇瓶初筛，得到五株摇瓶效价有大幅度提高的突变株。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that