A simple method for the collection of leucocytes from rat blood

Zusammenfassung Blutleukozyten von Ratten wurden mit menschlichem (normalem) Serum behandelt. Die Viabilität dieser Präparationen konnte dadurch demonstriert werden, dass die Zellen durch Phytohemagglutinin in «blast» Formen umgewandelt wurden.     

A simple method for sealing surgical skin wounds in young or new-born mice

Summary To close skin incisions in albino mice, a solution of polymethyl methacrylate in chloroform is recommended to be smeared over the wound, a procedure which virtually eliminates the incidence of maternal cannibalism.     

A simple method for heme isolation

Zusammenfassung Neues Verfahren für Häm-Isolierung durch Aceton-Fällung: Lösung in Aethylacetat-Eisessig und Auskristallisieren mit Wasserzugabe.     

A simple microcolorimetric method for the determination of pyruvate in 0,1 ml samples of blood

Riassunto Viene descritto un micrometodo colorimetrico alla 2,4 dinitrofenilidrazina che permette la determinazione del piruvato ematico in 0,1 ml di sangue. Il metodo è di semplice attuazione e soddisfa ai normali criteri analitici.     

A simple method for reconstructing serial sections

Résumé On décrit une méthode simple de reconstruction des sections sériées qui évite l'exécution fastidieuse d'un grand nombre de dessins. Bien qu'elle ne donne pas une image à trois dimensions, cette méthode est aussi utile qu'une autre pour étudier l'anatomie et les relations structurales par reconstruction de sections sériées.     

A rapid and simple method for the isolation of pure eosinophilic leukocytes from horse blood

Summary An improved and short method is described for the isolation of intact eosinophilic leukocytes from horse blood with high yield (1–1.5 g/20 1). Viability and purity of the preparations were verified by light and electron microscopy and by the trypan blue exclusion test. Isolated eosinophils were 98–100% pure, intact and viable, and they could be shown to phagocytise immune-complexes.The authors are indebted to the Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung for financial support.     

A simple enzymatic method for isolation of hepatocytes

Summary A new method is described for isolating hepatocytes by an enzymatic procedure: 0.05% bacterial collagenase in calcium-free Hanks' solution is injected into the liver tissue which is removed aseptically. The time required to prepare culture and risk of bacterial and fungal contamination is greatly reduced.     

A simple method for reduction of non-specific fluorescence

Résumé Cette technique pour la réduction de la fluorescence non-spécifique utilise diverses méthodes communes en une série spéciale. Premièrement, les anticorps fluorescents sont adsorbés avec la poudre du foie. Suivant la centrifugation, une colonne de Séphadex-25 est utilisée pour séparer la fluorescéine libre. La méthode contraire donne une fluorescence non-spécifique qui ressemble à celle obtenue avec une seule adsorption ou Séphadex filtration.     

Amino acid conjugates in blood and urine. I. A simple method for n-substituted amino acid determination

Zusammenfassung Eine neue, vereinfachte, elektrophoretische Trennungstechnik zur quantitativen Bestimmung von Aminosäuren in Körperflüssigkeiten in konjugierter Form oder als ninhydrin-negative Polypeptide wird beschrieben.     

A simple method for the preparation of diastereoisomeric phosphonodipeptides

Summary Diastereoisomeric mixtures of phosphonodipeptides, prepared from racemic dialkyl 1-aminoalkanephosphonates, were separated by ion-exchange column chromatography.     

A new simple assay for total blood lipids by refractometry

Summary A fast simple method for assay of total blood lipids has been devised by precipitating blood proteins with methanol and extracting lipids into diethyl ether. The change in refractive index of the methanol-ether due to dissolved lipids is measured in a refractometer.     

A simple method for estimating melanophore responses to drugs in fishes

Résumé On décrit une technique simple pour mesurer les réactions mélanophoriques dans la peau des poissons.     

A simple method for counting nuclei in the preimplantation mouse embryo

Summary An easy and rapid method of counting the number of cells in the preimplantation mouse embryo is described. The procedure increases the speed with which large numbers of embryos can be processed using a simple squash technique. Cell numbers are determined by exposing the embryos to the fluorescent DNA-binding dye, Hoechst 33258, removing the zona pellucida and simply squashing the embryo and counting the number of fluorescent nuclei. An increase in fluorescent intensity and maintenance of nuclear conformation of the squashed preparations are greatly improved by the use of the non-ionic detergent Triton X-100. Viability of dye-treated fertilized one-cell and blastocyst stage embryos is maintained at least up to day 13 of pregnancy following transfer of the embryos to the uteri of pseudopregnant recipients. Additional uses for this staining technique are discussed.Acknowledgment. Financial support was provided by NIH Grant HDD-06210 (KME) and by Basil O'Connor Starter Research Grant No. 5-379 from the March of Dimes Birth Defects Foundation (VEP). We thank Steven Halpern for help with the photography and Jon Flax for expert technical assistance.     

Intrasplenic infusion. A simple method for intraportal infusion in rats

Summary A simple and time-saving method for intraportal acute or chronic infusion in the rat by the insertion of a catheter into the spleen is described. This method has proved to be especially useful for performing chronic infusions in conscious animals.The author thanks Providencia Cruz for excellent secretarial assistance. Present address: Veterans Administration Center, Medical Research Division, San Juan, Puerto Rico.     

A simple method of cannulating the portal vein and obtaining multiple blood samples in the rat

Summary A simple and rapid technique for cannulating the portal vein in the rat is described. Multiple blood samples can be obtained or alternatively substrate may be infused through the cannula.Supported by the Clayton Foundation for Research, Houston, Texas. Common resource support was also provided by the Research Service of the Veterans Administration. To whom reprint requests should be addressed as follows.     

A simple method for counting nuclei in the preimplantation mouse embryo

An easy and rapid method of counting the number of cells in the preimplantation mouse embryo is described. The procedure increases the speed with which large numbers of embryos can be processed using a simple squash technique. Cell numbers are determined by exposing the embryos to the fluorescent DNA-binding dye, Hoechst 33258, removing the zona pellucida and simply squashing the embryo and counting the number of fluorescent nuclei. An increase in fluorescent intensity and maintenance of nuclear conformation of the squashed preparations are greatly improved by the use of the non-ionic detergent Triton X-100. Viability of dye-treated fertilized one-cell and blastocyst stage embryos is maintained at least up to day 13 of pregnancy following transfer of the embryos to the uteri of pseudopregnant recipients. Additional uses for this staining technique are discussed.     

A simple method for cultivating the early chick embryo in vitro

Summary A method for cultivating the early chick embryo in an artificial medium has been developed that permits the determination of the stage at the onset of the treatment and the continuous observation of the embryogenesis.Kungliga Fysiografiska Sällskapet, Lund, supported this work.