自同构群的阶等于其阶p倍的有限p-群

假设G为阶大于p~2的有限非循环p-群,如果G的阶整除G的自同构群Aut(G)的阶,则称G为LA-群。本文主要考虑满足p|G|=|Aut(G)|的有限p-群G,并且分类了满足这一条件的某些有限p-群类。     

Superconvergence for L2-projection in finite elements

Two classes of superconvergence points for L 2 projection u\-n in n degree finite elements on uniform grids are shown by element analysis method. Superconvergence points of u n are n+1 order Gauss point G n+1 for odd d≥1 (so the endpoint of each element is of superconvergent), and n+1 order pointset Z n+1 for even n ≥2 (which includes the endpoints and midpoint), respectively. Symmetry point is only an important part of them.     

邻香草醛缩胱氨酸双Schiff碱的合成及其对粟酒裂殖酵母细胞生长代谢的热动力学研究

用摩尔比为2:1的邻香草醛(C_8H_8O_3)与L-胱氨酸(C_6H_(12)N_2O_4S_2)反应,合成了一种新的双Schiff碱化合物--双{2-[(3-巯基丙酸钠)-2-亚胺基-甲基]-6-甲氧基-苯酚}(OVCS)。通过元素分析、红外光谱、核磁共振等手段对其组成和结构进行了表征,确定其化学式为Na_2(C_(22)H_(22)N_2O_8S_2),采用TAM air微量热仪测定了新合成的Schiff碱化合物(OVCS)在305.15 K时对粟酒裂殖酵母细胞作用的产热曲线;根据产热曲线计算了在OVCS作用下,粟酒裂殖酵母细胞生长代谢的最大发热功率p_(max)、速率常数k、传代时间tG、抑制率I和半抑制浓度C_(I,50)等热动力学参数。通过实验可以发现随着OVCS浓度的增加,粟酒裂殖酵母细胞的生长代谢速率常数k、生长代谢的总热效应Q_(total)、最大发热功率p_(max)均减小,抑制率I、达到生长代谢最大功率所需时间t_(max)、传代时间tG均增加等规律,半抑制浓度C_(I,50)为35.99 mg/L(或9.62×10~(-2)mol/L)。实验结果表明,OVCS对粟酒裂殖酵母细胞有抑制作用,且浓度越大,抑制作用越强。     

Finite Abelian group of |A(G)|=27 pq

we have discussed structures of Abelian groupG by order |A(G)| of automoorphism group and have obtained all types of finite Abelian grooupG when the order ofA(G) equals 2⁷ pq(p, q are odd primmes). Huang Benwen: born in Oct. 1948. Associate professor. Current research interest is in structures of finite group     

Studies on the reaction between Laccase ando-methoxyphenol by microcalorimetry

The reaction between Laccase ando-methoxyphenol have been studied by LKB-2107 batch microcalorimetry system. Thermodynamic parameters Δ _r H _m , ΔG _o , ΔG _T ^≠ and kinetic parameters (K _m ,k ₂) have been determined. The process of the reaction has been analyzed from changes in energy by using the transition state theory. Two methods for enhancing catalytic power of Laccase are proposed. The results shown that formation of an enzyme-substrate complex is “anticatalytic”. The enter and sole source of catalytic power is the stabilization of transition state; reactant-state interactions are by nature inhibitory and only waste catalytic power. Supported by the National Natural Science Foundation of China Wang Tianzhi: born in 1968. Ph D     

求解对流扩散方程的一类AGE方法

结合Crank-Nicolson格式和第二类Saul’yev非对称格式,设计求解对流扩散方程的交替分组显式方法.得到求解对流扩散方程的交替分组显式方法为该方法是绝对稳定的,且使用方便,适合并行计算,具有较好的精度.     

Effects of antisense CENP-B on the proliferation of HeLa (Tet-off) cells

In order to study the change of the expression of centromere protein B (CENP-B) caused by antisense transfection, proto-eukaryotically expressed fused protein GST-CENP-B (65 ku) was injected into mouse, and a peculiar anti-CENP-B serum MaCenpB was collected. A strain of transfected HeLa Tet-off cell HaCb, which contains antisense CENP-B expressing vector pBI-EGFP-as-CenpB, was prepared. Northern blot and Western blot were used to analyze the repression of internal CENP-B in transfected cells. According to the growth curve, the proliferation of HeLa (Tet-off) is repressed by antisense CENP-B, and the multiplication time is prolonged for 32.81 h. The analysis of flow cytometry revealed that, compared with HeLa (Tet-off), the G₁ cell population of HaCb is increased (ΔG₁= 9%) while S fraction is decreased (ΔS = 11%), but the G₂/M phase is nearly unchanged (ΔG₂/M=3%). In the meanwhile, the mitotic index of HaCb declines greatly compared with that of HeLa (Tet-off). Immunofluorescence showed that the assembling of centromeres in HaCb cell is arrested. These results suggest that a normal expression of CENP-B may be necessary for cell proliferation.     

Minus domination number in cubic graph

An upper bound is established on the parameter Γ -(G) for a cubic graph G and two infinite families of 3-connected graphs G k, G * k are constructed to show that the bound is sharp and, moreover, the difference Γ -(G * k)-γ s(G * k) can be arbitrarily large, where Г -(G * k) and γ s(G * k) are the upper minus domination and signed domination numbers of G * k, respectively. Thus two open problems are solved.     

Rapid induction of mRNAs forPC3 by partial hepatectomy and epidermal growth factor

The expression of immediate early gene plays a pivotal role in rat hepatocyte proliferation from G₀ to G₁ phases and the progression through G₁ phase of the cell cycle within several hours after 2/3 hepatectomy. We investigated the different gene expressions within 1 h after 2/3 hepatectomy by representational difference analysis. Sequence analysis indicated thatPC3 induced by NGF was a kind of immediate early gene and might be correlated with liver regeneration. Moreover, we found that 2/3 hepatectomy could induce the expressing ofPC3 mRNA by Northern blot with a peak 1–2 h after surgery. In primary cultures of rat hepatocytes, addition of EGF resulted in rapid and transient induction ofPC3 mRNA. It was first reported thatPC3 gene belongs to immediate early gene associated with liver regeneration.     

红色赤潮藻对球形棕囊藻的化感作用研究

红色赤潮藻(Akashiwo sanguinea)和球形棕囊藻(Phaeocystis globosa)都是近年来在我国近岸海域频繁引发赤潮的藻类。为了探索红色赤潮藻和球形棕囊藻之间是否存在化感作用及作用方式,本研究采用不同起始密度培养红色赤潮藻与球形棕囊藻,并将前者的完整细胞培养液、无细胞滤液、藻液超声破碎液分别与球形棕囊藻进行混合培养,分析红色赤潮藻对球形棕囊藻细胞生长的影响。结果显示,在红色赤潮藻细胞起始密度保持5×10⁵ cells/L的混合培养条件下,起始密度分别为5×10⁵、1×10⁶、2×10⁶、1×10⁷ cells/L的球形棕囊藻生长均受到明显的抑制;无论是含有红色赤潮藻完整细胞的培养液,还是其无细胞滤液或藻液超声破碎液,均对球形棕囊藻的生长具有显著抑制作用(P<0.01),抑制强度由强至弱依次为完整细胞培养液、无细胞滤液和藻液超声破碎液;相反,球形棕囊藻的存在对红色赤潮藻细胞的生长无显著影响(P>0.05)。因此,红色赤潮藻具有抑制球形棕囊藻生长...     

铁山港红树林高岭土沉积区虫害及互花米草调查与分析

为调查红树林大面积死亡的原因,以及虫害、互花米草对高岭土的响应,本研究在铁山港榄根村红树林区设立23个样方,调查样方内红树植物主要植食性害虫的种类和密度,以及植物叶片受损率;调查蛀干害虫在白骨壤树干上的分布规律,并统计互花米草的面积,从而分析高岭土对有害生物以及有害生物对红树植物的影响。结果显示,调查区域主要植食性害虫为柚木肖弄蝶夜蛾（Hybaea puera）、海榄雌瘤斑螟（Ptyomaxia sp.）和蛎盾蚧（Lepidosaphes sp.）。其中夜蛾主要分布在低潮位,盾蚧主要分布在中高潮位,斑螟虫口数量少,无明显分布趋势。在高岭土影响较小的低潮区,红树植物叶片的受损较为严重。本研究首次发现多毛梢小蠹（Cryphalus pilosus）危害白骨壤的树干,说明高岭土粘附在叶片表面一定程度上减缓食叶害虫的危害,但引发了蛀干害虫的发生。调查区域互花米草斑块约180个,面积约2.82 hm²,死亡红树林斑块内已有互花米草入侵,高岭土的淤积未对互花米草造成不利影响,相反在高岭土淤积严重导致红树林死亡的区域,互花米草最先入侵定殖。本研究为调查地白骨壤死亡的原因提供数据支持,并为后期人工修复提出科学建议。     

Thermodynamic study on elimination of platinum inclusions in phosphate laser glasses for inertial confinement fusion applications

A new method to calculate the formation enthalpies and free energies of compounds based on standard electromotive force of cation, φ0, and free energies of formation, ΔG2980, or formation enthalpies, ΔH2980, of chloride was proposed. A linear relationship between φ0 and ΔG2980, φ0 and ΔH2980, and ΔG2980 and ΔH2980 was found respectively. The parameters used for thermodynamic calculation were calculated. The calculated values of the formation enthalpies and the free energies of formation of platinum compounds, such as Pt3(PO4)2 and Pt(PO3)2, were given for the first time. The mechanism of eliminating platinum inclusions in phosphate laser glass by POCI3 gas bubbling was interpreted from thermodynamic aspect.     

广西海岸带互花米草入侵时空动态遥感监测研究

为全面了解和分析外来入侵物种互花米草(Spartina alterniflora)在广西海岸带的时空分布特征与扩散趋势,应用遥感与GIS技术手段,基于多源多时序高分辨率遥感影像对广西海岸带互花米草进行时空动态监测,并结合历史调查资料进行分析。结果表明：2001、2008、2010、2013、2016、2019年广西海岸带互花米草面积分别为46.90、204.05、331.23、608.75、776.99、1 320.79 hm²;2001-2019年,互花米草面积增加了1 273.89 hm²,年均增长率为20.38%。2019年,北海市海岸带互花米草总面积为1 317.06 hm²,占广西互花米草总面积的99.72%;钦州市互花米草面积为3.68 hm²;互花米草已入侵防城港市,面积约为0.05 hm²。丹兜海、铁山港湾、廉州湾及北海银滩至营盘镇近岸滩涂是互花米草集中扩张区,且仍处于快速增长阶段;防城港市企沙半岛东侧近岸滩涂及北海市涠洲岛均已出现互花米草零星斑块;广西山口红树林生态国家级自然保护区内互花米草扩散有所减缓,而广西合浦儒艮国家级自然保护区内仍处于增长阶段。广西海岸带互花米草入侵仍处于快速扩张状态,且呈由东往西扩散的趋势。本研究可为广西海岸带互花米草治理提供基础数据和理论指导,对湿地保护、管理和可持续发展具有重要意义。     

3种苏铁属植物总黄酮、总多糖含量及抗氧化活性研究

为探究锈毛苏铁(Cycas ferruginea)、叉孢苏铁(C.segmentifida)和石山苏铁(C.sexseminifera) 3种苏铁属(Cycas)植物的总黄酮、总多糖含量以及抗氧化活性,本研究采用超声波辅助法对其根、叶柄、叶、雄球花、茎干的总黄酮及总多糖含量进行提取,以DPPH自由基(DPPH·)、羟自由基(·OH)、超氧阴离子自由基(O^-₂·)清除率评价其抗氧化活性,并采用Pearson相关性分析法分析相关性。结果表明：锈毛苏铁、叉孢苏铁和石山苏铁叶的总黄酮含量较高,大小依次为锈毛苏铁(8.61 mg·100 mg^-1)>叉孢苏铁(7.82 mg·100 mg^-1)>石山苏铁(1.04 mg·100 mg^-1);茎干中总多糖含量最高,大小依次为叉孢苏铁(28.32 mg·100 mg^-1)>石山苏铁(24.43 mg·100 mg^-1)>锈毛苏铁(16.59 mg·100 mg^-1<...     

Construction and characterization of partiallyntrC-deleted mutants inAlcaligenes faecalis

To study the effect of ntrC gene product on the expression and regulation of other important nitrogen-fixing genes in Alcaligenes faecalis, partially ntrC-deleted mutants of A. faecalis have been generated. To start with, the ntrC gene of A. faecalis was cloned into a suicide plasmid pSUP202 to create a recombinant plasmid pSUM1. The ntrC gene in pSUM1 was then replaced by a lacZ-Km^r fragment resulted in the generation of a plasmid pSUM2. The lacZ fragment in pSUM2 was further removed and a plasmid pSUM3 produced. As a second step, the plasmid pSUM2 or pSUM3 was introduced into the wild type of A. faecalis A1501 by conjugation and two partially ntrC-deleted mutants A15CM1 (ntrC∷lacZ) and A15CM2 (ntrC ^- ) were obtained. To understand the regulatory effect of the NtrC on the expression of nifH and nifA, a nifH-lacZ gene or a nifH-lacZ gene was introduced into the ntrC^- mutant by conjugation. The results indicated that: (ⅰ) although the ntrC^-mutant was nif ⁺ , its nitrogen fixation activity was only 20% that of the wild type; (ⅱ) the ntrC^- mutant failed to grow on the medium containing nitrate as a sole nitrogen source; (ⅲ) the regulation of ntrC gene expression did not require its own product; (ⅳ) the expression of nifH in A . faecalis was positively regulated by the ntrC. Deletion of the ntrC resulted in the reduction of nifH expression or even totally inactivated nitrogen fixation; (ⅴ) there was no obvious influence on the expression of nifA in A. faecalis if the ntrC gene was deleted.     

Root and xylem ABA changes in response to soil drying in two woody plants

Two woody plants, Platycladus orientalise (tolerant to drought) and Acacia auriculi-formis (sensitive to drought), have been subjected to rapid and slow soil drying. ABA levels in their roots and xylem sap have been determined using radioimmunoassay (RIA, sensitivity is 0.4 pmol per assay vial) with a monoclonal antibody against ( + )-ABA. ABA contents of P. orientalise and A. auriculiformis growing in well watered soil are 0.3 and 2.5 nmol·gDW-1 in roots and 1.6 and 0.4 μmol in xylem saps, respectively. A rapid soil drying has been applied to these two plants with soil water content (SWC) being reduced to 0.02 and 0.06 g·gDW-1 respectively. Under such treatment, ABA was increased by 22 times and 2 times in roots and by 7 times and 34 times in xylem saps respectively for P. orientalise and A. auriculiformis. After rewatering for 6 d, ABA in roots and xylem sap of both species returned to control levels. When a slow soil drying was applied, SWC was reduced to 0.1 and 0.13 g·gDW-1 respectively for P. orientalise and A. auriculiformis. ABA was increased by 5 times and 1.6 times in roots and by 6 times and 19 times in xylem saps respectively for these two plants. ABA in roots and xylem saps decreased to near control levels 8 d after watering. Plant leaf water potentials of both plants hardly changed at times when root and xylem ABA showed substantial increase in response to soil drying. It is concluded that ABA levels in the roots and xylem saps of P. orientalise and A. auriculiformis are more sensitively regulated than leaf water potential in response to soil drying and can act as a chemical signal in root-shoot communications of the drought stress.     

广西珍稀濒危真红树植物小花老鼠簕分布及种群特征

为了填补珍稀濒危真红树植物小花老鼠簕（Acanthus ebracteatus）在广西分布特征的研究空缺,了解其分布、种群大小及群落特征,本文采用野外勘察与样方调查相结合的方法,对广西境内小花老鼠簕可能的分布区域进行全面调查。结果表明：在广西小花老鼠簕仅分布于防城港市江山半岛西南侧和黄竹江中游潮滩,总面积766 m²（分别为686 m²和80 m²）,共1 831株（分别为1 796株和35株）;小花老鼠簕分布在高潮线附近有海漆（Excoecaria agallocha）等相对高大的乔灌木遮挡区域,群落结构分层明显,均呈集群分布;江山半岛小花老鼠簕种群生长相对稳定。广西小花老鼠簕种群分布范围极小,生境狭窄,容易遭到人为干扰,处境严峻,亟需对其进行抢救性保护和物种恢复。     

Prescribing symmetric scalar curvature onS 2

The problem of prescribing scalar curvature in S2 is discussed, and the solvability of the e-quation - Δu + 2-Reu = 0 on S2, is given, where R∈C0(S2). It is known that there are some obstructions . Some new results are given by seeking a solution of the minimax type. For example, supposing that R is G symmetric and is constant on the set of fixed points on S2 under G (where G is a subgroup of O(3)), It is proved that the equation is solvable if and only if R is positive somewhere.     

HPV16E7-HSP70 Hybrid DNA Vaccine Induces E7-Specific Cytotoxic T Cells and Antitumor Immunity

0 IntroductionInbroicguhltat einxpge cDtaNtiAonvafcocri nper eivse ant ninegw aanpdp cruoraicnhg wdiits-heases . Due to the comparative weakness of cellulari mmunity that is purely induced by E7 DNA vac-cine,research on DNA vaccine is now focused onhowto enhance the i mmunogenicity of DNA vac-cine.It has been found in some research that thestrength of cellular i mmunity activity has things todo with the speed of antigen molecules’degrada-tion,i .e.the faster the degradation,the higher th…