集成微电极结构的芯片介电电泳富集过程研究

集成微电极结构的介电电泳芯片具有高效、非侵入式等优点,在生化样品操纵与分析中具有重要的实用价值和研究意义。从理论上分析了芯片介电电泳富集的影响因素,构建了5种微电极间距分别为20,40,60,80,100μm的微流控芯片,重点研究了微电极间距和流体流速对酵母菌细胞富集效率的影响。结果表明,当PBS缓冲液电导率50μs/cm,进样速度10μL/min,施加电压8 VP-P,8 MHz保持不变时,通过改变微电极间距,发现微距为目标细胞直径的4—8倍时富集效率最高,为90%左右;当电极间距为20μm,改变进样速度,其他条件保持不变,发现进样流速为30~50μL/min时,富集效率最高,达到87%以上。     

基于光诱导介电泳原理的粒子操纵研究

理论分析了溶液电导率和粒子直径对光诱导介电泳力的影响,针对光诱导介电泳对微球的操纵机理进行建模仿真。设计了光诱导介电泳芯片,通过激发光电导层的光电效应形成虚拟电极,同时施加高频交流电压,实现了对4μm和10μm聚苯乙烯微球的介电泳力操纵。最终微球在负介电泳力的作用下分别以8.8μm/s和35μm/s的速度运动。通过光诱导介电泳实现了对微球的无接触无损伤的操纵。     

交流电热流对导电岛纳米电极介电组装的影响

为了获得导电岛纳米间隙电极系统中纳米颗粒介电组装成的纳米粒子链的形貌规律,在所制备出的导电岛纳米间隙电极系统中进行了纳米颗粒介电组装实验。基于纳米颗粒介电操控理论,建立了导电岛纳米间隙电极组装体系的纳米颗粒介电组装模型,并通过数值仿真方法,对该介电组装模型中的电场分布和介电组装过程中纳米颗粒所受的介电泳力、交流电热流以及两者合作用的电动力学行为进行了分析,探究了交流电热流对导电岛纳米电极介电组装的影响。实验发现:在导电岛纳米间隙电极系统中纳米颗粒的介电泳组装具有两种模式——纳米间隙之间的体组装与电极和导电岛表面上的面组装,并且组装模式的强弱程度受频率的影响,进而造成组装形成的纳米粒子链的分布和形态的不同。仿真结果表明:当频率增大到350kHz时,纳米缝两侧电热流向发生反向;在频率为150kHz且缝宽为500nm时,会在整条纳米缝任意位置(端点处外)出现电热流旋涡,并且电热流旋涡会随着驱动频率的增加而减弱;随纳米缝宽度减小,电热流对纳米粒子介电组装的影响减弱。     

由电泳沉积ZIF-67薄膜制备高效染料敏化太阳能电池对电极

为了提高碳化ZIF-67薄膜制备的染料敏化太阳能对电极的光电性能,本研究通过碳化电泳沉积ZIF-67薄膜得到多孔碳电极,并进一步在多孔碳电极热分解氯铂酸得到负载铂的多孔碳复合电极。通过光电流-电压曲线、电化学阻抗谱和强度调制光电流谱等的测试,对比单独在FTO导电玻璃基底热分解氯铂酸得到的铂电极和单独碳化电泳沉积ZIF-67薄膜得到的多孔碳电极,研究了负载铂的多孔碳复合电极作为染料敏化太阳能电池对电极的光电性能。与其它两种对电极相比,以负载铂的多孔碳复合电极为对电极的染料敏化太阳能电池光电转换效率最高,因碳化ZIF-67薄膜的多孔电极具有较大的比表面积,能负载足够量的铂,从而提供更多的催化位点,具有更好的催化性能,从而使染料敏化太阳能电池的光电转换效率得到显著提升。     

基于ITO玻璃-PDMS复合式低电压电泳芯片的快速制备

提出了一种基于ITO玻璃-PDMS复合式低电压电泳芯片的快速制备新方法.该方法采用Protel软件绘制低电压电泳芯片沟道的形状,利用电路板加工技术加工沟道模具,并采用PDMS整体浇注方法制备芯片微沟道;采用光刻、湿法刻蚀等微加工技术方法对ITO导电玻璃进行加工,并制备出ITO阵列电极.最后经修饰、封装等工序得到基于ITO玻璃-PDMS复合式低电压电泳芯片.实验表明:采用该方法制备的ITO阵列电极线条清晰,沟道侧壁陡直、表面光滑.同时,采用该方法制备芯片周期短,不需要昂贵的制作设备.     

基于电压/频率转换的极简交流电压测量方法

为解决现有交流电压幅值测量方法中对模数转换器性能要求高、硬件电路复杂的问题,采用电压/频率 转换器将交流电压幅度信息调制成数字频率信号,通过分析数字频率信号,可计算出调制前的交流电压幅值。 推导出变换周期内电压/频率转换器的输出频率与输入交流电压幅值的数学关系; 在此基础上设计出仅含有电 压/频率转换芯片和一片双运放的交流电压幅值检测电路。实验结果表明,该方法正确,电路设计合理有效。 该方法利用数字频率信号易积分特性,将每个测试值均用于计算待测信号的幅度,提高了测试数据的利用率, 且简洁的电路设计提高了测量的稳定性。     

超声辅助微细电解制备微细圆柱体电极基础试验

分析了超声因素在微细电解制备微细圆柱体电极工艺中的作用机理,在自行研制的数字化微细电解加工系统上,以超声辅助微细电解的技术手段,利用初始直径400／μm的钨丝进行了微细圆柱体电极的制备试验．试验中重点分析了超声频率、加工电压、电极浸入深度等工艺因素对电极制备质量和制备效率的影响．试验结果表明,超声因素的介入可有效改善微细圆柱体电极制备的成型精度,且其制备效率可提高24．4％～41．2％．     

菲涅尔液晶透镜的制备及偏光织构研究

针对现有的液晶透镜制备工艺复杂、工作电压高等缺陷,提出了一种制备工艺简单、工作电压低的新型菲涅尔液晶透镜.用紫外光曝光光刻掩膜版,得到了相应的菲涅尔波带式电极,灌注TN型液晶并制成样品,其工作电压为3.0 V,占空比为1:1,偏压为1:1.施加频率为100 Hz的交流方波信号,用正交偏光显微镜在不同外加电压下对偏光织构进行了观察.结果表明,偏光织构随外加电压的改变呈连续性变化,电极结构存在边缘效应,且随着电压的增大,边缘效应越严重.     

基于Simplorer的正交矢量型锁定放大器的仿真与研究

提出了一种基于Simplorer7.0实现正交矢量型锁定放大器的仿真分析方法,并探讨了正交矢量型锁定放大器在芯片电泳非接触电导检测方面的应用,重点分析了相关参数,如激励信号频率,待测信号幅度、频率等,对芯片电泳谱检测的影响.仿真结果表明该正交矢量型锁定放大器具有卓越的微弱信号检测能力,能有效地实现芯片电泳信号的检测和拾取.     

电极形状对介电泳抛光影响的仿真研究

为了解决平面抛光加工中提高精度和效率的难题,根据中性微粒在非均匀电场中可以产生介电泳效应,提出了一种可以提高抛光均匀性和效率的介电泳抛光方法.讨论了介电泳理论及抛光原理,运用COMSOL软件仿真分析了不同电极的电场线分布.仿真结果表明:圆环70~90mm电极形状的电场线分布最不均匀.进行了相应电极的加工试验,试验结果表明:使用圆环70~90mm电极时材料去除率最大,达到0.573mg/min,相比传统CMP提升了19.9%.使用圆Φ60mm电极形状,工件材料去除最均匀、抛光效率最高,2.5h可以将Φ76.2mm硅片全部抛亮,比传统CMP提高了3.6倍,且最终表面粗糙度为0.97nm.在实际加工中,圆Φ60mm电极形状最适合Φ76.2mm硅片的介电泳抛光.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with