D16S539基因座中检出三带型等位基因及遗传分析

目的:在亲子鉴定案件中检出的D16S539基因座三带型.方法:提取个体不同组织样本DNA,经两种扩增试剂盒检测及DNA测序分析.结果:被检母亲D16S539基因座分型结果为9/10/11,阴阳性对照分型结果准确.结论:D16S539三带型非常罕见,该个体为正常成年女性,推测此三带型突变来源于杂合子局部的染色体重排或非整倍染色体.     

用基因扫描技术对新疆维吾尔族4个STR位点遗传多态性的分析

研究了新疆维吾尔族群体4个STR基因位点D5S18、D13S317、D7S820和D16S539的基因及基因型分布,获得4个基因库的群体贵传学数据。采用PCR扩增技术和基因扫描技术进行群体样本STR遗传结构分析,并与其他种族、人群的等位基因频率进行比较。结果表明,4个基因位点在新疆维吾尔族人群中均具有遗传多态性,4个基因座的基因型分布均符合Hardy-Weinberg平衡定律（P＞0．05）,不同人群基因频率分布存在一定的差异。     

北京地区汉族群体D1S1612 STR基因座的遗传多态性

目的首次调查中国北京地区汉族群体D1S1612基因座的等位基因频率分布.方法采用扩增片段长度多态性(Amp-FLP)分型技术对D1S1612 STR基因座进行检测.结果D1S1612检出9个等位基因,25种基因型.其STR基因座基因型频率分布符合Hardy-Weinberg平衡(P＞0.01).个人识别机率(DP)为0.901～0.927.结论D1S1612 STR基因座属高杂合度、高识别能力的遗传标记,可用于法庭科学亲子鉴定和个人识别.     

Minifiler基因座对陈旧骨检测效果及在南阳回族人群中的多态性分布

目的:探讨Minifiler 9个基因座对陈旧骨骼中的检测与分型效果,并调查此9个基因座在南阳回族人群中的多态性分布。方法:用改良酚-氯仿法提取陈旧骨DNA 10份,Chelex-100法提取南阳回族无关个体DNA 440份,Minifiler试剂盒基因座复合扩增,检测,计算样本基因型分型和基因多态性参数。结果:9个基因座在陈旧骨中的检出率为90%~100%。基因座在南阳回族人群分布范围H值在0.7342~0.8844之间,Pm值在0.0425~0.0953之间,PIC值在0.7100~0.8582之间,DP值在0.8973~0.9736之间,PE值在0.4688~0.7282之间。结论:Minifiler试剂盒9个基因座可作为陈旧骨微量DNA的参考检测基因位点,该基因座尤以FGA、D2S1338、D21S11在南阳回族人群中的基因多态性较好,个体识别率高,在法医实践中具有应用价值。     

简化基因测序技术在植物检材个体认定中应用初探

目的在涉及中草药真假辨识及个体识别的案件中很大比例的植物类物证都无法用形态学检验方法来鉴别,而植物DNA测序分析技术为我们提供了一种强有力的手段。方法通过对18个桂花植物样品进行DNA建库和高通量测序、序列多态性分析,评估其遗传多样性,并用基于单核苷酸多态性分析方法探讨了简化基因测序技术用于个体识别的案例。结果未知植物物证样品A与桂花1的匹配度最高,未知植物物证样品B与桂花4匹配度最高,未知植物物证样品B与桂花11的匹配度最高,该方法能成功识别3个盲测样品。     

FHIT基因微卫星不稳定性及人乳头瘤病毒感染与宫颈癌的关系

目的探讨FHIT基因微卫星不稳定性及人乳头瘤病毒感染与宫颈癌的关系.方法选取FHIT基因的两个微卫星多态标记,采用聚合酶链反应(Polymerase Chain Reaction,PCR)法对50例宫颈癌及40例宫颈上皮内瘤样病变组织进行微卫星不稳定性及HPV16感染的检测.结果在D3S1234和D3S4103位点上宫颈癌的MI发生率分别为18%、14%;CIN的MI发生率分别为12.5%、7.5%.宫颈癌与CIN在两个位点上的MI差异均无统计学意义.但宫颈癌在两位点上MI发生频率均高于CIN,且高级别CIN发生率高于低级别CIN.HPV感染的阳性组中MI发生率为31.8%,阴性组MI发生率为16.7%,但两组间MI的差异无统计学意义.结论宫颈癌中存在FHIT基因的MI,准确检测MI有利于对宫颈癌的预后作出客观估计,具有一定的临床意义.     

中国江苏地区汉人群五个高多态性短串联重复序列(STR)基因座基因频率分布调查

为选择有较好基因频率分布的短串联重复序列遗传标记,对中国江苏地区随机汉族人群的D5S818、D19S253、D12S391、D2S1338和D22S684 5个微卫星基因座进行了基因频率分布调查,并评估它们作为法医遗传标记在中国江苏地区汉族群体中的效能.采用Chelex-100法从中国江苏地区无血缘关系汉族个体的血样本105份中提取DNA,特异引物聚合酶链反应,产物经6%变性聚丙烯酰胺凝胶电泳分离,银染检测.这5个STR基因座在中国江苏地区汉人群均显具有高度遗传多态性并符合Hardy-Weinberg定律,它们的个人识别率(DP)范围从D5S818的0.892 8到D2S1338的0.952 7,非父排除率(PE)范围从D5S818的0.538 5到D2S1338的0.690 7不等.5个基因座的联合DP值和联合PE分别为0.999 997和0.991 8.在D22S684基因座,首次在汉人群报道的等位基因频率分布与英国Caucasian,Afro-Caribbean和Asian from the Indian 3个人群相比较,卡方检验有显著差异(P《0.005).为常规应用这8个STR遗传标记在中国江苏地区汉人群中进行亲子鉴定和个人识别提供了概率计算依据和群体遗传学数据.     

通过模拟现场提升《法医物证学》教学质量

根据法医物证学实践性和实战性强的学科特点,为了把＂学＂与＂用＂有机融合,培养实战性和实用型的高级法医学人才,我们对传统的《法医物证》实验教学进行了大胆改革,探索一种以《刑事科学技术学》的现场勘查为主导,通过＂模拟现场＂将《法医物证学》实验教学贯穿于其中的教学模式。经过五年的教学实践,在推动《法医物证学》教学和提升教学质量中发挥重要的作用。     

D2S1338基因座在华北汉族的遗传多态性分析

目的：了解D2S1338基因座在华北汉族人群中的基因分布情况,并与国内外相关统计资料进行对比,给科研和办案提供法律依据。方法：373个无关华北汉族个体和40个家系检材经Identifiler五色荧光复合试剂盒PCR扩增,ABI PRISM 3100DNA毛细管电泳仪检测,使用GeneScan(3．1．2)和Genotyper软件分析。结果：华北汉族中观测到D2S1338基因座有13个等位基因,52种基因型,He为0．855,PIC为0．84,DP为0．964。出现频率最多的等位基因是23、24。华北汉族与黑种人和白种人比较,基因分布有极显著差异。结论：D2S1338基因座具有很高的个体识别能力,在法庭科学中具有很高的应用价值。     

D18S535 STR基因座的遗传多态性研究

调查中国北方地区汉族群体D1 8S535基因座的等位基因频率分布。方法 :采用扩增片段长度多态性 (Amp FLP)分型技术对其STR基因座进行检测。结果 :D1 8S535检出 9个等位基因 ,2 7种基因型。该STR基因座基因型频率分布符合Hardy Weinberg平衡 (P >0 0 2 5)。个人识别机率 (DP)为 0 92 7。结论 :D1 8S535STR基因座属高杂合度、高识别能力的遗传标记 ,可用于法科学亲子鉴定和个人识别     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that