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1.
棒曲霉UA—2木聚糖酶的蔗渣固态发酵   总被引:3,自引:0,他引:3  
棒曲霉(Aspergilusclavatus)UA-2固态发酵蔗渣产木聚酶的培养基为每克蔗渣加营养液5ml,初始pH8.5.营养液的适宜组成为每升含:NH4NO310g、K2HPO46g、MgSO4·7H2O0.75g、CaCl20.75g、FeSO4·7H2O11.25mg、MnSO4·H2O3.75mg、ZnSO43.0mg、CoCl24.5mg.在上述培养基中接入其湿重10%(W/W)的新鲜的UA-2种子曲,28℃培养108h,其木聚糖酶,滤纸降解酶和羧甲基纤维素酶的活力分别为2695.6、28.5和42.7u/g蔗渣.酶反应的最适pH5.0,最适温度50℃;在pH4.0~11.0内酶活性十分稳定.50℃保温1h,酶活剩余55%,8℃下放置23d,活力几乎不变.磷酸盐,半胱氨酸对酶有激活作用,EDTA和对氯汞本甲酸对酶有抑制作用  相似文献   

2.
采用均匀设计对肌苷工程菌枯草芽孢杆菌株Q2901-4-35(SGr)的最佳发酵条件进行了研究。确定发酵培养基配方为(质量分数)葡萄糖12% ,药用酵母粉1.4% ,硫酸铵1.3% ,玉米浆0.5% ,Na2HPO4·12H2O 0.5% ,KCl0.6% ,MgSO4·7H2O 0.22% ,CaCO3 2.0% ,尿素0.2% (分消)pH7.0,在上述发酵培养基中发酵产苷达15.20g/L,该菌株的产苷能力比在原发酵培养基中的产苷能力提高了10% 。从而证明均匀设计实验所给回归模型对发酵条件的选择具有重要的指导意义。  相似文献   

3.
产胞外木聚糖酶青霉菌发酵条件的正交设计试验   总被引:1,自引:0,他引:1  
正交设计试验结果表明,青霉菌(Penicillium sp.m8) 胞外木聚糖酶活力达92.96 U/mL,合适的产酶发酵条件为:培养基(g/L),麦草粉40,(NH4)2SO4 4.44,KH2PO4 1.0,MgSO4 ·7H2O0.5,NaCl0.3,Tween 80 3.0,CaCO3 1.0;每亳升培养基接种2.0×105 个孢子,28 ℃,水浴振荡培养4 d(120r/min)。  相似文献   

4.
氯化铜对滇紫草细胞色素形成的影响   总被引:7,自引:0,他引:7  
滇紫草细胞于M-9培养液中悬浮培养以形成紫草色素。当M-9培养液中CuSO4·5H20由相同浓度的CuCl2·2H2O取代后,色素产量比对照提高了58.7%,CuCl2·2H2O的最适浓度为30μmol/L。滇紫草细胞的继代培养基B—5中的CuSO4·5H2O取代后,培养所得的细胞接种于M-9培养液中,色素产量比对照提高了32.08%,CuCl2·2H2O的最适浓度为0.6μmol/L.CuCl2·2H2O对细胞生长无不良影响。M—9培养液中,铜于色素形成的初期,可快速吸附于细胞上,这一现象为CuCl2·2H2O促进紫草色素合成机制的深入研究提供参考。  相似文献   

5.
北冬虫夏草液体深层发酵最适营养的研究   总被引:5,自引:0,他引:5  
通过摇瓶单因子和正交设计试验结果。液体培养的最佳营养条件为:葡萄糖2%,食用糖1%,酵母膏1%,蛋白胨1%,NH4Cl0.2%,MgSO4.7H2O0.05%,KCl10.1%,100立升发酵罐结果,碳,氮源分别为食用糖3%,蛋白胨1.5%。  相似文献   

6.
测定了293.2~323.2K间的7个温度下电池GE|H2O(1-w)+En(OH)2(w)+HCl(m)|AgCl-Ag的电动势,由此求得了上述7个温度下,m=0.0020,0.0050,0.0100,0.0500,0.1000mol/kg的HCl在0~90%乙二醇—水二元混合溶剂中的平均活度系数γ±与Pitzer参数;HCl自水向各组成En(OH)2—H2O混合溶剂中的标准迁移自由能,标准迁移熵和标准迁移焓.最后从分子间相互作用的角度对HCl在该混合溶剂中的热力学行为给予了理论解释.  相似文献   

7.
复合型纳滤膜的制备及表征   总被引:12,自引:0,他引:12  
在聚砜基膜上用界面聚合法制备了两种复合型纳滤膜PA01和PA02。在0.3MPa下,PA01膜对MgSO4溶液和FeCl3溶液的脱直 96.0%和78.0%,水通量分别为8.5(L.m^-2.h^-1)和7.9(L.m^-2.h^-1);PA02膜对MgSO4ipwkiyw (2.0g.L^-1)和CaCl溶液(0.5g.L^-1)的脱盐率分别为98.0%和88.8%,水通量分别为1.0(L。m^  相似文献   

8.
CaCl2*6H2O-MgCl2*6H2O多温截面的研究   总被引:3,自引:0,他引:3  
用步冷曲线法研究并绘制了CaCl2·6H2O-MgCl2·6H2O多温截面图,实验显示该截面有两个四相转熔反应,其转熔温度分别为25.0℃和22.0℃.结果表明,CaCl2·6H2O-MgCl2·6H2O混合盐可用于低温相变储热.  相似文献   

9.
采用均匀设计的方法,优化胡萝卜素产生菌红酵母R-93发酵培养基的营养条件,经回归分析和发酵试验得出最佳培养基配方(%)蔗糖为1.0,蛋白胨为1.25;(NH4)2SO4为1.0KH2PO4为0.28;MgSO4.7H2O为0.025,在此条件下,发酵指数可达到0.262g(干菌体).(L.h)^-1。  相似文献   

10.
本文提出以Ag2SO4—Al2(SO4)3—MgSO4作催化剂,在H2SO4-H3PO4混酸溶液中快速测定废水COD的新方法.实验确定的最佳条件是:Ag2SO4:Al2(SO4)3:MgSO4=1∶1∶1(质量比),H2O4:H3PO4=5∶1(体积比),回流时间0.5小时,通过对废水COD的测定,取得与标准法相近的结果.  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

13.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

14.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

15.
正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-  相似文献   

16.
We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-  相似文献   

17.
正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that  相似文献   

18.
For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the  相似文献   

19.
Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of  相似文献   

20.
<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with  相似文献   

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