Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method

A 1-step silver-staining technique, requiring only 2 min to perform, is described for the differential staining of nucleolus organizer regions. A protective colloidal developer is used to control the reduction of the silver.     

Variable positions of nucleolus organizer regions in Bovidae

Summary Silver NOR staining has been applied to cattle (Bos taurus L.), goat (Capra hircus L.) and sheep (Ovis aries L.) chromosomes. The sites of silver NORs showed variation within the family Bovidae probably due to a reciprocal translocation event.     

Detection of nucleolus organizer regions (NOR) in the chromosomes of the domestic pig (Sus scrofa domestica L.)

Summary In the domestic pig (Sus scrofa domestica L.) nucleolar organizer regions (NOR) were detected by a combined silver-Giemsa method (Ag-G). The main sites of NORs are the secondary constrictions of chromosomes 8 and 10. Sometimes an additional NOR was observed near the centromere of 1 homologue of chromosome 11. Associations of NORs were seen only between chromosomes 10 and at a very low frequency.     

Detection of nucleolus organizer regions (NOR) in the chromosomes of the domestic pig (Sus scrofa domestica L.)

In the domestic pig (Sus scrofa domestica L.) nucleolar organizer regions (NOR) were detected by a combined silver-Giemsa method (Ag-G). The main sites of NORs are the secondary constrictions of chromosomes 8 and 10. Sometimes an additional NOR was observed near the centromere of 1 homologue of chromosome 11. Association of NORs were seen only between chromosomes 10 and at a very low frequency.     

Interphase studies with a simplified method of silver staining of nucleoli

Summary A simple silver staining method is presented providing a rapid and reliable technique for the selective staining of nuclear structures synthesizing ribosomal RNA (18S and 28S RNA).The critical advice of Prof. Dr W. Krone is gratefully acknowledged.     

Interphase studies with a simplified method of silver staining of nucleoli.

A simple silver staining method is presented providing a rapid and reliable technique for the selective staining of nuclear structures synthesizing ribosomal RNA (18S and 28S RNA).     

控制性低中心静脉压下原位肝移植术中凝血功能的变化

目的 观察控制性低中心静脉压（Low central venous pressure,LCVP）技术下,接受原位肝移植患者手术中各时期及术后凝血功能的变化. 方法 选择我院原位肝移植手术患者40例,随机分为正常CVP组（C组）和LCVP组（L组）,采用相同全身麻醉方式后,C组维持CVP在8～12 mmHg,L组将CVP降至4～5 mmHg,维持动脉收缩压≥90 mmHg,检测和记录肝移植各期和术后血小板水平、凝血功能相关指标、术中出血量、相关血制品输注量、术后引流情况及气管导管拔出时间. 结果 两组患者术前血小板及凝血功能各指标差异无统计学意义;C组患者中无肝期和新肝期1 h与无肝前期比较,PLT、Fg明显减少,TT、PT、APTT明显延长,L组患者移植各期PLT、TT、PT、Fg水平差异无统计学意义;L组与C组患者比较,移植各期PLT、Fg值增高,PT、APTT明显缩短;术中L组出血量较C组减少;患者血小板、凝血酶原复合物、冷沉淀输注总量均明显减少;L组患者肝上、肝下间隙引流管引流量减少,拔管时间缩短. 结论 LCVP技术应用于原位肝移植手术中,可改善术中凝血功能障碍,减少术中各类血制品输注及相关风险,有利于术后凝血功能的迅速恢复和缩短引流管放置时间,促进术后患者恢复.     

An improved method for estimating the activity of a mouse with the photoswitch

Summary A new method for estimating the activity of a mouse by means of the photoswitch is contrived. This device can follow the activity with high fidelity and notable sensitivity, as compared with the conventional method.     

An improved method for estimating the activity of a mouse with the photoswitch

A new method for estimating the activity of a mouse by means of the photoswitch is contrived. This device can follow the activity with high fidelity and notable sensitivity, as compared with the conventional method.     

Studies on GABA accumulation induced byγ-glutamyl-hydrazide in regions of rat brain following treatment with a tyrosine hydroxylase inhibitor and 6-hydroxy-dopamine

Summary Reduction of DA receptor activity via depletion of DA stores does not seem to influence GABA turnover in the forebrain and in the DA cell body rich region of the midbrain.This work was supported by a grant (MH25504-03) from the National Institute of Health, USA, and by Magn. Bergvalls Stiftelse and Funds from the Karolinska Institute, Stockholm, Sweden.     

Brain chopping: a new method for the rapid removal of newborn rat brain.

A new multi-bladed air-driven guillotine is described. This device removes newborn rat heads and slices them into several thin wafers, which facilitates freezing and permits easy regional dissection.