瞬态电磁脉冲对细胞跨膜电位的影响

我们初步研究了瞬态电磁脉冲的非热生物效应,取得了第一手实验数据,为进一步探索其机理提供基础［１］．低强度瞬态电磁脉冲非热效应的源发点为细胞膜,脉冲对细胞跨膜电位的影响是导致其生物效应的原因之一［２］．所以需要了解电磁脉冲激发的细胞跨膜电位,以及瞬态电磁脉冲特性对跨膜电位的影响．我们采用简化的细胞电学模型计算得到不同特性的瞬态电磁脉冲对细胞跨膜电位的影响,结果具有普遍指导意义．１　简化的细胞电学模型　　在实验系统中,电磁脉冲的频率范围不是很宽,采用的细胞种类也有限,可以统一采用简化的细胞电学模型进…     

低强度瞬态电磁场脉冲作用于不同动物细胞形成电穿孔的实验比较

电穿孔是由电磁脉冲作用引起细胞或生物组膜半通透性暂时或永久性丧失出现穿孔的现象 .目前电穿孔技术已广泛应用于生物技术、基因工程及临床医学等很多领域 ,而其研究多集中于针对单个或数个瞬时高强度脉冲 (电场强度为 10 2 ～ 10 3ｋＶ/ｍ ,脉冲作用时间一般为 10 - 6 ～ 10 - 3ｓ)作用下细胞或组织膜的穿孔现象 ,与此相关的机理分析及实验研究已较为详尽[1～ 4 ] .近 10年来 ,我们一直致力于弱电磁场的非热生物效应的研究 ,并采用电场强度几ｋＶ/ｍ、脉冲宽度几个ｎｓ～ 10 0ｎｓ的低强度瞬态电磁脉冲对生物样品进行照射实验 ,结果发现…     

瞬态电磁脉冲以淋巴细胞遗传及免疫功能的影响

通过以人的T－淋巴细胞为受试对象，以其结合绵羊红细胞形成E－玫瑰花结的百分率为指标，测试了不同波形参数的脉冲电磁场，不同照射时间对E－玫瑰花结形成率的影响；并讨论了瞬态电磁脉冲（TEMP）对长白猪巴细胞转化率等遗传学效应的影响，为解释低强度瞬态电磁脉冲对细胞产生非热效应作用的机理提供了可靠的依据。     

电穿孔——解释生物体中非热生物效应的关键机理

介绍了细胞膜电穿孔的形成过程和基本应用，报道了瞬态电磁脉冲和生物细胞相互作用的一些重要实验结果。采用电穿孔机理解释了细胞膜破裂的原因。     

瞬态电磁脉冲对淋巴细胞遗传及免疫功能的影响

通过以人的 T-淋巴细胞为受试对象 ,以其结合绵羊红细胞形成 E-玫瑰花结的百分率为指标 ,测试了不同波形参数的脉冲电磁场、不同照射时间对 E-玫瑰花结形成率的影响 ;并讨论了瞬态电磁脉冲(TEMP)对长白猪淋巴细胞转化率等遗传学效应的影响 ,为解释低强度瞬态电磁脉冲对细胞产生非热效应作用的机理提供了可靠的依据     

瞬态电磁脉冲对淋巴细胞遗传及免疫功能的影响

通过以人的T-淋巴细胞为受试对象,以其结合绵羊红细胞形成E-玫瑰花结的百分率为指标,测试了不同波形参数的脉冲电磁场、不同照射时间对E-玫瑰花结形成率的影响;并讨论了瞬态电磁脉冲(TEMP)对长白猪淋巴细胞转化率等遗传学效应的影响,为解释低强度瞬态电磁脉冲对细胞产生非热效应作用的机理提供了可靠的依据.     

利用电穿孔提高抗肿瘤药物细胞毒性的初步研究

作者运用电磁脉冲使细胞电穿孔并结合抗肿瘤药物观察细胞的毒性 ,比较了电穿孔组与对照组细胞的抗肿瘤药物胞质毒性的差异 ,发现电穿孔组比对照组的胞质毒性显著提高 .结果说明 ,利用电穿孔可提高抗肿瘤药物的细胞毒性 ,为治疗肿瘤提供了新的途径 .     

低强度瞬态电磁脉冲对淋巴细胞影响的实验研究

从低强度瞬态电磁脉冲对淋巴细胞转化率、T-淋巴细胞免疫能力等影响的实验入手，通过人的T-淋巴细胞为受试对象，以其结合绵羊红细胞形成E-玫瑰花结百分率为指标，测试了不同波形参数的脉冲电磁场、不同照射时间对E-玫瑰花结形成率的影响，为解释低强度脉冲电磁场对细胞产生非热效应作用的机理了可靠的依据。     

低强度瞬态电磁场下动物细胞的电穿孔效应

用低强度瞬态电磁场处理动物细胞后,一些胞内蛋白可以溢出细胞,荧光标记抗体蛋白（ＩｇＧ）可以进入细胞,通过扫描电镜观察到红细胞膜上有电致孔洞,证实了低强度瞬态电磁场作用能起电穿孔。     

瞬态电磁脉冲对细胞增殖的影响

实验采用有丝分裂指数测定法及＾３Ｈ－ＴｄＲ掺入法,研究瞬态电磁脉冲对人外周血淋巴细胞及艾氏腹水癌细胞增殖的影响。结果发现,没有植物凝集素ＰＨＡ时,１００ｋＨｚ和２００ｋＨｚ瞬态电磁脉冲对淋巴细胞有丝分裂指数和＾３Ｈ－ＴｄＲ掺入量无显著影响;有ＰＨＡ时,１００ｋＨｚ瞬态电磁脉冲促进淋巴细胞摄取＾３Ｈ－ＴｄＲ,细胞有丝分裂指数增加,２００ｋＨｚ瞬态电磁脉冲抑制淋巴细胞摄取＾３Ｈ－ＴｄＲ,细胞有丝分裂指     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with