The effect of kryptopyrrole on the porphyrin auxotrophic strains ofBacillus subtilis

Zusammenfassung Nachweis, dass Kryptopyrrol zwar die Porphyrinsynthese inBacillus subtilis Wildtyp stimuliert, nicht aber das Wachstum von Porphyrin-Mangelmutanten dieses Bakteriums ermöglichen kann.     

Stimulation of sporulation by ppApp in a conditionally asporogenous rifampin-resistant mutant ofBacillus subtilis

Summary Addition of ppApp to Sterlini-Mandelstam medium stimulates sporulation of a conditionally asporogenous rifampin-resistant mutant ofBacillus subtilis to the same extent as the effect of 4 amino acids. Mutant cells sporulating in the presence of amino acids also produce 2 phosphorylated nucleotides one of which comigrated with ppApp on PEI thin layer chromatogram.Acknowledgment. This work was supported in part by a grant from Research Corporation, New York, New York.     

A sporulating strain ofBacillus popilliae

Zusammenfassung Durch Behandlung mit Chloroform ist es möglich,Bacillus popilliae in flüssigem Medium zur Sporenbildung zu veranlassen. Diese Bakterien können zur Bekämpfung von Insekten, derPopilliae japonica, benützt werden.     

Regulation of ppApp synthesis during sporulation of a conditionally asporogenous rifampin mutant ofBacillus subtilis

Summary The intracellular synthesis of ppApp in a conditionally asporogenous rifampin-resistant mutant ofBacillus subtilis stimulated to sporulate in the Sterlini-Mandelstam (SM) medium supplemented with ppApp is negligible and comparable to that of the non-sporulating culture. The formic acid extract from the sporulating culture stimulates sporulation of the mutant in SM medium.This paper was presented at the Annual Meeting of the American Society for Microbiology in Dallas, Texas, in March 1981. This work was supported in part by a grant from Research Corporation, New York, N.Y., USA.     

Effect of proteins on the reversibility of the thermal inactivation ofBacillus subtilis α-amylase

Zusammenfassung Die Reaktivierung von thermisch desaktivierter-Amylase ausBacillus subtilis durch Zusatz von Guanidin-HCl oder Harnstoff oder von Guanidin mit Zusatz verschiedener Proteine wurde untersucht.     

Inhibition of L-Alanine-induced germination ofBacillus cereus spores by theophylline

Riassunto In spore diBacillus cereus, la teofillina inibisce reversibilmente la germinazione dal-alanina ma non l'attivazione che lal-alanina esercita sulla germinazione da inosina. Il possibile significato di questi dati è discusso.     

Penicillium auxotrophic mutants can be detected by suing xanthene dyes

Summary Auxotrophic mutants ofPenicillium spp. have been directly isolated after mutagenic treatment from agar plates containing Xanthene dyes. They grow as characteristic small colored colonies. Some strains were tested and they showed a differential response depending on the Xanthene dye used.     

Lipid accumulation by a cellulolytic strain ofAspergillus niger

Lipid accumulation by a cellulolytic mold,Aspergillus niger, was studied. The amount of lipid accumulated ranged from 13.6–16.6% on various carbon sources, namely glucose, xylose, avicel (microcrystalline cellulose) and bagasse (a natural lignocellulosic substrate). Neutral lipids, phospholipids and glycolipids of the mycelia varied from 41.0–46.2%, 34.9–38.4% and 18.7–22.6% of total lipids, respectively. Unsaturated fatty acids comprised around 80% of total fatty materials with linoleic and oleic acid predominating. Of the four nitrogen sources tested, NH₄Cl was the best source for lipid synthesis from cellulose (bagasse). Optimum temperature range for growth and lipid synthesis was 25–30°C.     

Lipid accumulation by a cellulolytic strain of Aspergillus niger.

Lipid accumulation by a cellulolytic mold, Aspergillus niger, was studied. The amount of lipid accumulated ranged from 13.6-16.6% on various carbon sources, namely glucose, xylose, avicel (microcrystalline cellulose) and bagasse (a natural lignocellulosic substrate). Neutral lipids, phospholipids and glycolipids of the mycelia varied from 41.0-46.2%, 34.9-38.4% and 18.7-22.6% of total lipids, respectively. Unsaturated fatty acids comprised around 80% of total fatty materials with linoleic and oleic acid predominating. Of the four nitrogen sources tested, NH4Cl was the best source for lipid synthesis from cellulose (bagasse). Optimum temperature range for growth and lipid synthesis was 25-30 degrees C.     

Stimulation of sporulation by ppApp in a conditionally asporogenous rifampin-resistant mutant in Bacillus subtilis

Addition of ppApp to Sterlini-Mandelstam medium stimulates sporulation of a conditionally asporogenous rifampin-resistant mutant of Bacillus subtilis to the same extent as the effect of 4 amino acids. Mutant cells sporulating in the presence of amino acids also produce 2 phosphorylated nucleotides one of which comigrated with ppApp on PEI thin layer chromatogram.     

A new actinomycin-like antibiotic produced by a mutant strain ofStreptomyces indicus

Zusammenfassung Aus einer Mutante vonStreptomyces indicus Chakrabarty sp. nov. wurde das neue Antibiotikum MT-10 (orange-gelbe Kristalle) isoliert. Die antibakterielle und antifungische Aktivität wurde untersucht und festgestellt, dass MT-10 mit Actinomycin D verwandt ist.     

Activation of nisin production by UV-irradiation in a nisin-producing strain ofStreptococcus lactis

Zusammenfassung Isolierung von Mutanten mit erhöhter Nisin-Produktion aus einem weiterenStreptococcus lactis-6 Stamm durch UV-Bestrahlung (9000 erg/mm²). Die Produktion des Antibiotikums nahm nach der ersten Bestrahlung im 50%, nach der zweiten Bestrahlung um 100% zu, während nach der dritten eine Abnahme beobachtet wurde.

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