Reduction of efferent renal nerve activity by propranolol in rabbits]

During intravenous infusion of propranolol (0.3--0.8 mg/kg) in the Rabbit, systolic arterial pressure is decreased (-6.5 +/- 4.2 mmHg) and electrical activity recorded from central end of the renal nerve is reduced significantly (-8.7 +/- 14.-%) with regard to the activity obtained, at the same pressure levels, by hemorrhage (+7.9 +/- 6.4%; p less than 0.25) or by intravenous infusion of the peripheral vasodilator sodium nitroprusside (+14.1 +/- 9.9%; p less than 0.01).     

Tubular and glomerular nephropathy induced in rabbits by injection of a heterologous antitubular basement membrane serum]

A nephropathy affecting both tubules and glomeruli has been induced in Rabbits by the injection of an heterologous immune serum directed against Rabbit tubular basement membrane. It differs from experimental antiglomerular basement membrane nephropathy by the fixation of antibodies and C3 on tubular basement membrane and by the occurrence of glucosuria.     

Study of the cortical field potentials produced by weak light stimulation in adult rabbits]

On a chronic preparation of an adult Rabbit, the cortical visual evoked response, produced by a weak luminous stimulation activating only the scotopic system is characterized by the suppression of the early surface-positive phase and by a lengthening of the latencies of the following deflection. The profile of the fields of potential corresponding to the various defletions in relation to the depth, has been investigated.     

The effects of a heterologous antibody against a crude lipoprotein lipase fraction in mice]

The antigens contained in a crude lipoprotein-lipase fraction from the Rabbit adipose tissue have no effect on Mice, even after daily injections for several months. But they induce an immune reaction in Sheep, allowing one to produce several immune sera with a high physiological activity, without any specific barrier between Rabbit and Mouse. After total absorption of these immune sera by normal Rabbit serum, the remaining antibodies have an activity against esterasic lipoproteins. Small doses of these antibodies induce in Mice marked hyperplastic and degenerative reactions of the adipose tissue and an extensive lysis of the pancreas. A correlative high lymphocytic activation, producing B and T immunoblasts, is induced.     

Activity of rabbit heart on thromboxane synthetase]

The soluble and microsomal fractions of Rabbit myocardium are not able to induce the synthesis of thromboxanes. On the contrary, they inhibit the thromboxane synthetase of various sources. The chemical structure of the active constituent responsible for this activity is not yet known: it is probably neither of an enzymatic nature, nor a protein of high molecular weight.     

Immuno-electron microscopic localization of fibronectin on rat mast cells

Summary Rabbit anti-rat plasma fibronectin (pFN) causes histamine release from rat mast cells in the presence of complement. Fibronectin (FN) on rat mast cells, as shown by immuno-electron microscopy, is principally localized on cell folds, so they may play a role of attachment in the matrix of connective tissue.Acknowledgment. This work is supported by grants (No. 56770014 and No. 544018) from the Ministry of Education, Science and Culture of Japan.     

Simultaneous isolation of rabbit glomerular and tubular basement membrane]

A method allowing simultaneous isolation of Rabbit alomerular and tubular basement membranes has been perfected. Rabbit tubular basement membrane has also been obtained by tryptic digestion of the renal cortex. Electron microscopic observation and biochemical analysis show that the contamination of the membrane preparations by cellular components is negligible.     

嫦娥三号“玉兔号”巡视器遥操作中的关键技术

2013年12月14日,"嫦娥三号"成功地将月面着陆器和"玉兔号"巡视器送抵月球,这标志着我国已完成了探月工程中"落"月的重要阶段.嫦娥三号任务的一个关键组成部分是由地面遥操作中心控制"玉兔号"巡视器在月面非结构化环境中进行巡视和科学探测,而遥操作技术正是该组成部分成功实施的关键."玉兔号"巡视器遥操作技术主要包括巡视器的导航定位、月面地形重构、行驶路径规划和机械臂探测等关键步骤,这些步骤相互支撑与融合,保证了嫦娥三号任务的顺利实施.本文在对上述4个方面关键技术的发展和应用现状进行总结的基础上,阐述了相关的主要技术途径及其应用特点,分析了各种技术在"玉兔号"月面巡视与科学探测任务实施中的发挥的重要作用,并评述了它们的发展潜力和应用前景,对中国探月工程以及后续火星探测工程中的遥操作具有一定的指导意义.     

Role of lysosomes, microtubules and microfilaments in the mechanism of the lactogenic action of prolactin in the rabbit mammary gland]

The lysosomotropic agents, ammonium chloride and chloroquine, added to the culture medium of pseudopregnant Rabbit mammary gland, did not inhibit the initiation of casein synthesis by prolactin. By contrast, they considerably reduced the down-regulation of prolacting receptors. Converse-y, colchicine totally blocked the lactogenic action of prolactin without altering the down-regulation of the receptor. Cytochalasin B inhibited only partly the lactogenic action of prolactin while it has no effect on the down-regulation of the receptor. These data suggest that the degradation of the prolactin-receptor complex in lysosome is not a compulsory step in the mechanism of prolactin action. The integrity of microtubules but not of microfilaments is required for prolactin to initiate casein synthesis. These elements of the cytoskeleton are not strictly involved in the down-regulation of the receptor.     

X-ray diffraction of the collagen contained in blood vessels]

An X-ray diffraction study has been undertaken on Human and Rabbit blood-vessels. Vessels are stretched 500% and the diagrams present a great analogy with those obtained from collagen. Thus, it appears, that it is possible to determine by X-Rays some pathological anomalies of the collagen in vessels.     

Binding of cytophilic rabbit IgG to homologous hepatocytes.

Rabbit liver cells were able to bind cytophilic monomeric and polymeric homologous IgG via their Fc receptor binding sites (FcR). On the other hand, non-cytophilic rabbit IgG did not bind to hepatocytes, even after its aggregation. The present findings suggest that FcR on rabbit liver cells are specific for cytophilic monomeric IgG but do not significantly bind non-cytophilic, polymeric IgG.     

Synthesis of sulfated glycosaminoglycans by the three cell types of the rabbit cornea in culture.

Rabbit corneal cells were cultivated for 21 days and then exposed to Na235SO4, a precursor of sulfated glycosaminoglycans (GAG). All 3 cell types of the cornea, the fibroblasts, the epithelial as well as the endothelial cells, synthesize GAG. The fractionation-patterns of the epithelial and endotherlial GAG are almost identical and differ clearly from the one of fibroblastic GAG.     

Evolution of intrabacterial glucides during differentiation of two types of feces in the colon of the domestic rabbit]

In the Rabbit, during excretion of hard feces, a correlation exists between the stock of bacterial carbohydrates at the caecal level and the intensity of the bacterial lysis observed in the proximal colon.     

Apparent transitory hyperthyrotropinemia of mothers and newborn infants related to the presence of maternal immunoglobulins

Some newborns infants (screened for congenital hypothyro?dism) and their mothers were found to present an apparent isolated hyperthyrotropinemia. A large molecular size material (150 000 dalton) was found to carry the apparent immunoreactivity and was characterized as immunoglobulins, capable of binding TSH as well as Rabbit IgG. These maternal immunoglobulins are passively transferred to the newborn infants and interfere in various radioimmunoassays.     

Inhibition of microsomal Na+ K+ ATPase of the brain by extracts of Mansonia altissima]

Water extracts of the bark of Mansonia altissima var altissima inhibit the activity of the Na+, K+-ATPase of Rabbit brain microsomes. The kinetics of hydrolysis of ATP show non-competitive inhibition analogous to that produced by ouabain.     

Membrane damage by coelomic fluid from Holothuria polii (Echinodermata)

Rabbit erythrocyte membranes lyzed by Holothuria polii coelomic fluid, observed under the electron microscope, present lesions consisting of irregular holes which are heterogeneous in size (ranging from 50 A to 250 A) and ultrastructurally different from the ring-like structure produced by human complement. The protein pattern associated with the lyzed membrane was also examined.     

Binding of cytophilic rabbit IgG to homologous hepatocytes

Summary Rabbit liver cells were able to bind cytophilic monomeric and polymeric homologous IgG via their Fc receptor binding sites (FcR). On the other hand, non-cytophilic rabbit IgG did not bind to hepatocytes, even after its aggregation. The present findings suggest that FcR on rabbit liver cells are specific for cytophilic monomeric IgG but do not significantly bind non-cytophilic, polymeric IgG.We thank Mrs Sanda Maghiar for her excellent technical assistance.     

In vivo and in vitro incorporation of 3H-thymidine in the uterus of the rabbit during pseudopregnancy

During pseudopregnancy in the Rabbit the DNA synthesis in the uterine epithelium is very reduced. These results conjoined with the decrease of the mitotic index and previous ultrastructural observations allow to think that multinucleated cells proceed mainly by cell fusion.     

Immunological demonstration of large quantities of glycogen or of a glycogen-like substance in human embryonic colon cells and in colon carcinomas by means of rabbit antisera raised against a strain of Escherichia coli 013]

Rabbit antisera raised against a strain of E. coli 013, with a strong antiglycogen activity, were tested on human fetal and normal adult colons, on colon carcinomas, and on colon tumor cells in culture (HT29). Only very rare granules were present in adult normal colons when tested with the immunofluorescence method. In faetal colons, in 12 out of 14 carcinomas, and on HT29 cells, the immunofluorescent reactions were similar to those observed in normal liver. The reactions were negative after previous treatment with alpha-amylase. They were inhibited with glycogen, with phenol-alcohol, perchloric, and trichloroacetic extracts from faetal colons, and with a tumor trichloroacetic extract. The extracts precipitated with anti-E. coli 013 antisera. They had a strong inhibiting activity in a radioimmunoassay test with labeled glycogen. The extracts from normal adult colons did not precipitate with the antisera and they had no inhibiting activity in either immunofluorescence and radioimmunoassay tests.     

Respiratory responses in heat-exposed rabbits: Inhibition of tachypnoea offset by increase in tidal volume

Summary Rabbits in which thermal panting has been inhibiting by previous cold exposure or by water deprivation respond to a raised ambient temperature with an increase in tidal volume. By so doing, they are able to maintain a minute volume appropriate to their thermoregulatory requirements.