白唇鹿睾丸生精上皮的组织学观察

研究了白唇鹿睾丸和生精上皮的组织学结构，钭基表上皮的细胞组合为八个不同的时相，并描述了各时相的生精细胞组合特点，发现白唇曲细精中级生精细胞的数量比较少，且细胞的排列较为疏松。     

白唇鹿血浆性激素的测定与分析

本文用放射免疫法对锯茸期白唇鹿血浆中的睾酮、雌二醇、孕酮.雌酮等性激素的含量连行了测定,结果发现,睾酮含量较低,雌二醇等雌激素的含量较高。     

白唇鹿(Cervus albirostris)生殖器官的形态观察

白唇鹿为我国的特产动物,仅分布于四川、青海、甘肃、西藏的高寒高原地区,是青藏高原的特有种。关于白唇鹿的生态生物学资料已有一些报道。由于高原寒冷的原因。它的繁殖时间较其他鹿类晚,对其生殖器官的解剖特征,至今未见报道。本文根据得自青海祁连县两个白唇鹿成体的生殖器官进行解剖观察,以提供解剖学资料。     

白唇鹿生殖器官解剖特征的研究

为了探讨白唇鹿的生殖生理,保护和增殖这一珍贵资源动物,本文对其生殖器官进行了解剖学研究。发现雄性睾丸之纵轴与体轴成直角位置,实质棕黄色,小叶不明显;精囊腺表面光滑而发达,前列腺和尿道球腺短小;阴茎呈圆柱状,龟头前端有4—5个裂口,膨大时呈莲花瓣状。雌性卵巢为偏平的椭圆形;子宫角为绵羊角状,基部融为一体,每侧角有4—5个长椭圆形的肉阜排成一列,怀孕时可随胎儿的发育而增长,子宫体短小,子宫颈坚实,阴道宽大.白唇鹿的以上特征与其它动物有着明显差.     

白唇鹿与牦牛:高寒地区的好邻居

白唇鹿与牦牛都栖息在青藏高原．都喜欢在海拔3500米以上的高寒地区生活。但是，由于平时没有往来，互不了解，还曾发生了邻里之间的不愉快。后来，由于及时沟通，消除了误解，成为了好邻居。     

青石爬鮡头骨形态的观察

对青石爬鮡的头骨形态作了描述.结果表明,其头骨不仅具有鲶形目鱼类的共有特征,而且具有一些高度特化的特征,如头骨极为平扁,腭骨、颌骨特化并高度发达,鳃弓的基-下鳃区骨化程度很低,大部分为结缔组织.这些特化是与其山溪激流生活相适应的.     

梅花鹿染色体G-带及模式图

采用胰酶G-带技术,对梅花鹿的G-带进行了研究,参照ISCNDA(1989)的国际家畜细胞遗传学命名体制,绘制了梅花鹿的G-带模式图.结果表明:梅花鹿单倍染色体组所显示的染色体数目为248条,包括X和Y染色体.     

梅花鹿FGF10基因的生物信息学分析

应用生物信息学的方法对梅花鹿FGF10基因的核苷酸和氨基酸序列进行了初步的生物信息学分析,包括理化性质分析、信号肽和跨膜结构域分析、磷酸化位点和疏水性分析、蛋白质二级结构分析、功能结构域分析以及系统进化分析.结果表明:梅花鹿FGF10基因编码213个氨基酸,蛋白相对分子量为23.84kD,为碱性不稳定蛋白;存在信号肽和跨膜结构域;共有26个磷酸化位点;二级结构主要由α螺旋、β转角、延伸链和随机卷曲组成.具有FGF典型的FGF结构域.系统进化分析显示,梅花鹿FGF10与哺乳动物FGF10相似性较高,并且与牛、羊在亲缘关系上最相近.为梅花鹿FGF10基因的结构和功能的进一步研究打下了坚实的理论基础.     

海南坡鹿血浆纤维蛋白原的测定及其意义

采用饱和氯化纳溶液法，通过分光光度计测定海南坡鹿血浆纤维蛋白原的光密度，计算出它的血浆纤维蛋白原的百分浓度．测定结果，海南坡鹿血浆纤维蛋白原的含量为（４２０±２０）ｍｇ／１００ｍｌ．     

饲养条件下水鹿（Cervus unicolor）行为的研究

从１２１头水鹿中，按不同性别和年龄选取９头水鹿，用定量记录的方法，观察了１２９６０次行为。结果表明，不同性别和年龄组的个体在常规行为上均有差异，其中成年雄鹿（鹿角硬化或脱落）和成年雌鹿在各项行为频次有明显的不同；成年雄鹿和长茸鹿间各项行为频次也表现出明显的不同。白昼，其休息和摄食行为频次较高，其余行为均受摄食状况的直接影响。晨、昏各出现一次水鹿活动的高峰。在摄食和性行为中，水鹿表现出社群等级。发情期，水鹿间存在着性信息联系。水鹿的某些特别行为，与性活动有关。     

吉林双阳梅花鹿成纤维细胞生长因子10的克隆和基因分析

根据多个物种的成纤维细胞生长因子10的序列,设计了特异的引物,从吉林双阳梅花鹿子宫cDNA库中得到了梅花鹿成纤维细胞生长因子10,并应用多种数据库和软件对其进行了分析．     

梅花鹿骨形态发生蛋白BMP4基因的克隆与序列分析

通过提取梅花鹿肝脏总RNA以及采用RT-PCR技术,成功克隆梅花鹿骨形态发生蛋白BMP4基因序列,并对其核酸序列、蛋白质序列,功能保守区、亲疏水性进行生物信息学分析,同时构建序列进化树以及部分结构的三维模型.该序列已提交Genbank(索取号:HQ877675).该研究可为日后通过基因工程手段表达重组梅花鹿BMP蛋白并探究其药理作用提供基础.     

四川梅花鹿食性的研究

对四川梅花鹿食性的研究表明：鹿采食活动在晨昏和夜间进行，采食的植物种类共计212种，杨柳科，桦木科，蓼科，小蘖科，蔷薇科，豆科，胡秃子科，忍冬科，菊科，禾本科，莎草科的植物是其食物的主要来源，占采食植物总数的67％，鹿在不同的季节对食物基地和采食的有明显的选择性，10月至翌年4月，鹿常在2700－3200m处的土欠下面舐食含铵盐的土，饮水在晨昏和夜间进行，水源为河流，小溪和山泉。     

Natural diet and food habitat use of the Tarim red deer,Cervus elaphus yarkandensis

In order to determine the natural diet and food habitat use of Tarim red deer (Cervus elaphus yarkandensis), a study was carried out in Qiemo, Xinjiang , China from October 2000 to June 2001. Direct observation combined with faecal analysis method was used to determine the natural diet of red deer. 15 different species of plant were identified as food items. Among them, 13 species of plants were identified in winter diet and 9 species in summer. Red deer consumed a wider range of species in winter because of their nutrient requirement as well as the shortage of food and the scarcity of high-quality forage in the study area. Phragmites communis, Glycyrrhiza inflata and populus diversifo-lia were frequently present in the deer's diet whenever in winter and summer. Among them, Phragmites communis was the most abundant plant in the area and was included in the deer's diet. Observation on food selection frequency of captive Tarim red deer showed that Populus diversifolia was the first preferred species. However, this food was limited in the study area. Five food habitat types were found in the study area according to plant association: (1) Phragmites communis-Tamarix ramosissima association, (2) Tamarix ramosissima-Halostachys cas-pica association, (3) Tamarix ramosissima-Phragmites communis association, (4) Populus diversifolia-Phragmites communis association, (5) Burned area. Among them, Phragmites communis-Tamarix ramosissima association (reed meadow and reed marsh) was preferred to other types within the study area whenever in summer and winter. Dense reed cover could reduce the chance of detection from predator and obstruct attack from predator. Furthermore, under the cover of the reed, Tarim red deer was protected from direct solar radiation during the hours of hot day in summer. The reed meadow and marsh was preferred, presumably because the red deer could minimize their movements while searching for food, water and cover.     

Natural diet and food habitat use of the Tarim red deer, Cervus elaphus yarkandensis

In order to determine the natural diet and food habitat use of Tarim red deer （Cervus elaphus yarkandensis）, a study was carried out in Qiemo, Xinjiang , China from October 2000 to June 2001. Direct observation combined with faecal analysis method was used to determine the natural diet of red deer. 15 different species of plant were identified as food items. Among them, 13 species of plants were identified in winter diet and 9 species in summer. Red deer consumed a wider range of species in winter because of their nutrient requirement as well as the shortage of food and the scarcity of high-quality forage in the study area. Phragmites communis, Glycyrrhiza inflata and populus diversifolia were frequently present in the deer＇s diet whenever in winter and summer. Among them, Phragmites communis was the most abundant plant in the area and was included in the deer＇s diet. Observation on food selection frequency of captive Tarim red deer showed that Populus diversifolia was the first preferred species. However, this food was limited in the study area. Five food habitat types were found in the study area according to plant association： （1） Phragmites communis-Tamarix ramosissima association, （2） Tamarix ramosissima-Halostachys caspica association, （3） Tamarix ramosissima-Phragmites communis association, （4） Populus diversifoliaPhragmites communis association, （5） Burned area. Among them, Phragmites communis-Tamarix ramosissima association （reed meadow and reed marsh） was preferred to other types within the study area whenever in summer and winter. Dense reed cover could reduce the chance of detection from predator and obstruct attack from predator. Furthermore, under the cover of the reed, Tarim red deer was protected from direct solar radiation during the hours of hot day in summer. The reed meadow and marsh was preferred, presumably because the red deer could minimize their movements while searching for food, water and cover.     

短尾拟鲿头骨的研究

短尾拟鲿头骨结构中无顶骨、后耳骨、下鳃盖骨,有续软骨存在;犁骨、前颌骨;齿骨上密生铺石状牙齿;上颌骨和腭骨分别特化成粒状和棒状小骨,用以支持触须;舌颌骨、方骨、中翼骨、外翼骨发育良好;前鳃盖骨失去鳃盖作用而参与悬器形成;上枕骨具成对的三叉神经叉脊支神经孔。