A simplified method for isocratic HPLC analysis of polyamines

Summary A simple technique is described for the separation and analysis of polyamines in tissues and body fluids, utilizing precolumn clean-up on disposable CM-cellulose columns, followed by an automatable HPLC procedure. Complete separation and analysis takes 12–15 min per sample with sensitivity in the pmole range.Acknowledgments. Supported by USPHS HD-09713, EPA R811621010 and by Research Scientist Development Award DA-00006 to TAS.     

A simplified silver impregnation method

Resvmer Método sencillo de impregnación argéntica para degeneración walleriana. Buenos resultados en cerebros guardados mucho tiempo en formol. Se discute la acción del permanganato y la hidroquinona-oxálico como tratamiento previo. Se omite reacción con plata acuosa y se elimina el alcohol de la plata amoniacal.     

A simplified method for silanization of doublebarrelled ion-sensitive microelectrodes

Summary A simplified procedure is described for silanization of one shank of a doublebarrelled microelectrode. this procedure makes it possible to make more functioning electrodes in a shorter time.     

A simplified method of nerve organ culture

Résumé Une méthode simplifiée de culture d'organe nerveux est décrite. Elle réduit le temps consacré à la nourriture et permet un changement rapide du milieu nutritif. Elle convient aux études microscopique (m. à contraste de phase) et elle est relativement peu coûteuse.     

A rapid HPLC method for determination of adenylate energy charge

A simple procedure is described for separation and analysis of adenine nucleotides in tissue extracts, utilizing anion exchange HPLC. Determination of AMP, ADP, and ATP takes 10 min per sample.     

Primary tissue cultures from organ fragments: A simplified method

Summary Organ fragments washed in Ca⁺⁺ and Mg⁺⁺-free saline, treated with trypsin and placed directly into culture flasks adhered within seconds to the vessel surface. If the fragments were suspended in culture medium before they were added to the flasks, they did not adhere. This technique permits the rapid attachment and subsequent growth of the primary tissue cultures.Adapted from a thesis submitted in partial fulfillment of the requirements for the degree of Master of Science at Florida Atlantic University.Acknowledgment. This work was carried out in the laboratory of J.X. Hartmann, whom I thank for his generosity and helpful discussion.     

A simplified method for reading hemagglutinations on a flat-bottom microtitration plate in the mouse H-2 assay

Summary Kaliss' method for determining hemagglutination titer of mouse alloantiserum was modified to a simpler form. It became possible to read the hemagglutination directly on a flat-bottom microtitration plate using an inverted type microscope. Sensitivity of the modified method was almost the same as that of the original one. This method seems to be useful especially for the larger scale assay of H-2 specificities.Contribution from the National Institute of Genetics. Supported in part by grants-in-aid (No. 111510, 111504, 112105) from the Ministry of Education, Culture and Science, Japan.Acknowledgments. The excellent technical assistance provided by Miss M. Kuranou, Mr K. Sakakibara and Miss M. Tsuyuki is gratefully acknowledged.     

A new method of scenario analysis for strategic planning

We first present scenario analysis as a qualitative forecasting technique useful for strategic planning. Then we develop an overview of the two classes of methods for scenario analysis described in the literature. Based on both classes, a new method is developed which especially fits the needs of strategic planning. The method can be divided into three stages: 1. Determination of compatible scenarios, 2. Determination of scenario probabilities, and 3. Determination of main scenarios. An example is given to illustrate the method.     

A simplified genesis of quantum mechanics

The bewildering complexity of the history of quantum theory tends to discourage its use as a means to understand or teach the foundations of quantum mechanics. The present paper is an attempt at simplifying this history so as to make it more helpful to physicists and philosophers. In particular, Heisenberg's notoriously difficult derivation of the fundamental equations of quantum mechanics, or later derivations of its statistical interpretation are replaced with shorter and more direct arguments to the same purpose. As the implied amputations and distortions do not imply major anachronisms, they should facilitate the grasping of the main historical steps without excluding a reasonable assessment of their historical or logical necessity.     

A simplified assay for cyclic AMP using protein kinase binding

Summary The protein kinase binding assay for cAMP was modified by substitution of adsorption by QAE cellulose for the membrane filtration. This modification obviates the variation of recovery of cAMP with the volume of buffer used to wash the filter. The assay is reproducible and technically simpler than those currently employed.Acknowledgment. This work was partially supported by grants HL-16583 and HL-18827 from the National Heart and Lung Institute.     

Interphase studies with a simplified method of silver staining of nucleoli

Summary A simple silver staining method is presented providing a rapid and reliable technique for the selective staining of nuclear structures synthesizing ribosomal RNA (18S and 28S RNA).The critical advice of Prof. Dr W. Krone is gratefully acknowledged.     

Interphase studies with a simplified method of silver staining of nucleoli.

A simple silver staining method is presented providing a rapid and reliable technique for the selective staining of nuclear structures synthesizing ribosomal RNA (18S and 28S RNA).     

Primary tissue cultures from organ fragments: a simplified method.

Organ fragments washed in Ca++ and Mg++--free saline, treated with trypsin and placed directly into culture flasks adhered within seconds to the vessel surface. If the fragments were suspended in culture medium before they were added to the flasks, they did not adhere. This technique permits the rapid attachment and subsequent growth of the primary tissue cultures.