Die Isolierung von Cortisol-21-sulfat aus dem Urin von Cushingpatienten vor und nach totaler Adrenalektomie

Summary Cortisol-21-sulphate has been identified in the urine of three patients with Cushing's syndrome before and after total adrenalectomy (substitution with 200 mg F orally). The identification was based upon the following data: Identical behaviour of the standard cortisol-21-sulphate and the unknowns in two different paper chromatographic systems; positive methylene blue reaction and UV-contact-photogram; identical absorption spectra in concentrated sulphuric acid; correspondence with free cortisol of the steroid moiety liberated by dioxan solvolysis and formation of adrenosterone following treatment with chromic acid. The data support evidence that cortisol-21-sulphate may originate not only from the human adrenal gland but also from extra-adrenal sources, presumably the liver.     

Die Isolierung von Ribonukleinsäuren aus menschlichen Knochenmarkzellen

Summary By use of bentonite and sodium dodecylsulfate, which bind or inhibit ribonucleases, pure RNA preparations were obtained from human bone marrow. Phenol extraction at 4 °C yielded cytoplasmic RNA, which could be separated into 3 main peaks by sucrose density gradient centrifugation. Sedimentation coefficients of these peaks were about 30S, 19S, and 5S, respectively. Re-extraction of the phenolic interphase at 65° gave a polydisperse ribonucleic acid, sedimenting between 2S and 19S. This RNA is characterized by template activity and is supposed to be biologically active m-RNA.     

Zu Hermann von Helmholtzens mechanischer Grundlegung der Wärmelehre aus dem Jahre 1884

Ohne Zusammenfassung Vorgelegt von S. Flügge     

Zur Frage der Neubildung von Primordialfollikeln aus dem Keimepithel während der Geschlechtsreife im menschlichen Ovar

Summary Findings in the human ovary confirmed the fact already pointed out in animal ovaries that under the influence of oestrogens the germinal epithelium is capable of forming new oocytes. Whether the germinal epithelium of the mature woman can form new oocytes under normal conditions cannot be determined as yet, but the present findings allow the conclusion that neo-oogenesis during the reproductive period of women seems possible. Further investigations, now in progress, are required to determine this possibility.     

Nachweis von Reflex-Bluten bei Lampyriden (Coleoptera) mit Hilfe der „Tracer”-Methode

Summary The very short time (5–9 min) between injection of radiophosphorous into the hemolymph of adult fireflies (Lampyridae) and outflow of radioactive droplets after irritation demonstrates with certainty that reflex-bleeding is involved. Labelling the secretions of dermal glands would require much more time (several h or even 1 day.

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Institut für Angewandte Zoologie der Universität Bonn. Die Untersuchungen wurden 1972 während eines Aufenthaltes vonW. J. Kloft als Visiting Professor an der University of Florida durchgeführt.     

Ein einfaches Verfahren zur Sichtbarmachung von Glasmikroelektroden mit Hilfe von Fluorescein

Summary Glass microelectrodes were filled with 3M KCl containing 2 g of sodium fluoresceine/l. The glass-tips of the electrodes are visible under the microscope in a blue activating light.     

Isolierung eines neuen Guanidin-Derivates aus der Geissraute,Galega officinalis L.

Summary A new guanidine derivative has been isolated from the seeds ofGalega officinalis L., for which the structure 4-hydroxy-3-methyl-but-2-enyl-guanidine-(1) was proposed.     

Bestimmung des zeitlichen Verlaufes der Erschlaffung der kontraktilen Elemente mit Hilfe des Spannungs-Frequenz-Diagrammes

Summary By plotting the tension developed during maximal summation of muscle twitches against the stimulus interval, a curve can be drawn which has the same time course of the falling phase of the active state as determined by the method of quick release.     

Desazetylierung von Cardenoliden mit der Zinkazetatmethode

Summary Some examples of transesterifications of cardenolidacetates to acetyl-free glycosides with zincacetate are reported. The method is advantageous for the deacetylation of acetates with base-resistant acetyl groups or with special base sensitivity.

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56. Mitteilung über Herzglykoside. 55. Mitt. s. Helv. chim. Acta51, 1317 (1968).     

Isolierung von Charcot-Leydenschen Kristallen

Summary A method is described for isolation of Charcot-Leyden crystals from high eosinophil-count blood specimens or from eosinophil rich exudates. Once the crystals have been formed, isolation is accomplished by lysing the blood cells with an enzyme and a wetting agent.

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Die vorliegenden Untersuchungen wurden durch ein Stipendium des Schweizerischen Nationalfonds zur Förderung der wissenschaftlichen Forschung ermöglicht.     

Darstellbarkeit doppelbrechender Lipoidtropfen aus dem Urin und pathogenetische Auffassungen der Lipoidurie

Summary (1) No double refractile Iipoid droplets can be obtained by alcohol ether extraction from urine or from urinary protein as it is possible from blood serum.(2) The conclusion is drawn that the double refractile lipoids droplets in urinary sediment do not pass through the glomeruli and therefore are not reabsorbed by the tubuli, but that they come from the own lipoproteins of the epithelial cells of renal tubuli and pass into urine by dissolution and desquamation of these cells.     

Beschleunigtes Wachstum von Karpfen in Aquarien mit Hilfe biologischer Wasserklärung

Summary Keeping carps (Cyprinus carpio) according to a new method in plastic containers (40 1) in a closed water cycle with biological cleaning yielded a quick increase in weight at a water temperature of 25°C.     

Proteolytische Enzyme aus dem Darmtrakt der Hornisse (Vespa orientalis F.)

Summary Two proteinases from the digestive tract of the oriental hornet larvaVespaorientalis were investigated. One hydrolyzes the chymotrypsin substrates ATEE and APNE, the other one the trypsin substrates BAEE and BANA. They belong to the serine proteases, and split B-chain of oxidized insulin in a way similar to that of vertebrates.

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Der Deutschen Forschungsgemeinschaft sei für finanzielle Unterstützung gedankt.     

Eine Methode zur titrimetrischen Bestimmung des Eisens im Blut mit Hilfe von Komplexon

Summary The volumetric determination of total iron in blood by titration with Complexone III is described. The method is simple, rapid, and accurate but not sensitive enough to permit the estimation of iron in blood-serum alone.     

Ein neues Alkaloid aus dem Mycel einesClaviceps purpurea-Stammes. Zur Biogenese von Ergocristin

Summary A new alkaloid from the lyophilized mycelia of aClaviceps purpurea strain, N-[N-(d-Lysergyl)-l-valyl]-l-phenylalanyl-d-prolin-lactam, has been isolated and characterized. From its fast methanolysis it is deduced that the previously reported N-(d-Lysergyl)-l-valinmethylester is actually an artefact. The biogenetic significance and its possible role in biogenesis of Ergocristine is discussed.     

Mechanismus der Reaktion von Invertseifen mit isolierten Zellen

Summary Reaction equilibria were established between an invert soap on the one side and carbon, lecithin, erythrocytes and yeast cells on the other side. From the parallelism of the invert soap-lecithin and the invert soap-erythrocytes curves a specific reaction of the invert soap with the lipoids of the erythrocytes may be concluded, whereas the invert soap-yeast cells curves yield a pure adsorption isotherm corresponding to the invert soap-carbon curve.     

Gleichzeitiger Nachweis der 4. Aktionssubstanz mit dem Polarographen und am Froschherzen

Ohne Zusammenfassung     

Zur Methodik der elektrophoretischen Untersuchung der Proteine der Speicheldrüsensekrete

Summary Several methods have been studied for concentrating the human saliva in order to get reliable electrophoresis patterns. The best method is a dialysis in a cellophane tube against veronal buffer (pH 8.2) followed by vacuum exsiccation.     

Clausius' mechanische Grundlegung des zweiten Hauptsatzes der Wärmelehre aus dem Jahre 1871

Ohne Zusammenfassung Vorgelegt von S. Flügge