不同pH值的酸处理DNA溶液的拉曼光谱分析

通过拉曼光谱研究了经不同pH值的酸处理后的DNA溶液．小牛胸腺DNA被配制成pH6．0、pH5．0、pH4．0、pH3．0、pH2．0、pH1．0的酸性溶液，经24h充分水合后测试其拉曼光谱．实验结果表明，DNA分子内部产生了明显的质子化，其拉曼特征频率和强度均发生了变化，而且不同的pH值致使它们的变化程度也不同．对DNA分子结构的影响包括磷酸骨架基团，脱氧核糖及碱基．随着酸性的增强，DNA的构型也发生变化：在pH6．0、pH5．0时DNA溶液为标准B构型，而在pH4．0、pH3．0、pH2，0的DNA溶液出现B和C构型共存，到pH1．0时出现了Z构型．从pH3．0碱基开始质子化，逐渐地GC碱基对之间形成了Hoogsteen型氢键，AT碱基对之间的氢键发生断裂，双螺旋结构遭到破坏，而脱氧核糖也受到不同程度的损伤．     

萘酚绿B与DNA相互作用的FT-SERS研究

利用傅立叶变换———表面增强拉曼光谱(FT SERS)研究了萘酚绿B(NGB)与小牛胸腺DNA(ctDNA)的相互作用. 在银胶体系中, NGB分子1436,1358~1333,1080~1043,987,672 cm-1等处的拉曼信号有较显著的增强, 表明NGB以萘环吸附到银胶粒子的表面. 加入ctDNA之后, NGB分子的部分SERS带发生位移, 或强度进一步增加, 部分SERS带信号趋于消失, 说明NGB分子可能以静电力作用键合到DNA沟槽和通过插入作用等两种不同的模式与DNA发生了相互作用.     

DNA主链构象与脱氧鸟苷组分构象相关性的喇曼分析

对小牛胸腺ＤＮＡ纤维样品溶解３ｈ内的空间构象变化过程进行了喇曼光谱分析，结果显示了表征脱氧鸟苷糖环折迭不同类型的６７２ｃｍ＾－１与６８３ｃｍ＾－１峰的同时存在，与ＤＮＡ分子主链不同特征峰的同时出现之间有着明显的联系。     

Abnormal behavior of fluorescence quenching of gramicidin A inphase transition of lipid vesicles by hypocrellin B——Does anelectron transfer inverted region exist?

The fluorescence of gramicidin A(GA) in lipid bilayer may be quenched by Hypocrellin B. When the phospholipids undergo a phase transition from gel to liquid crystalline phase, the reaction rate decreases unusually. This phenomenon has been analyzed by Marcus’ photoinduced electron theory, and an electron transfer inverted region hypothesis is proposed, which may adequately explain this abnormal phenomenon.     

紫外辐射对Fei鱼精DNA溶液影响的拉曼光谱研究

报道了鲱鱼精DNA水溶液的拉曼光谱及其分别被UVA(320-400?nm)和UVA&UVB(280-400?nm)两个区段紫外辐射后的拉曼光谱.实验中所用的紫外辐射强度与中国南京地区冬季日光辐射强度相当.实验表明,在仅用UVA区段紫外辐射时,短时间内未见DNA分子结构有明显的变化;而UVA&UVB区段的紫外辐射则对水溶液中的DNA分子造成一定的损害,照射时间愈长损害愈大,首先受到损害的是嘧啶碱基和脱氧核糖.     

Optimization of hypocrellin B derivative amphiphilicity and biological activity

To satisfy the dual requirements of the fluent transportation in blood and the affinity to the target tissues of vascular diseases, hypocrellin derivatives with optimized amphiphilicity are expected. In this work, 3-amino-l-propanesulfonic acid and 4-amino-l-butanesulfonic acid substituted hypocrellin B, named compounds 1 and 2, were designed, synthesized in high yields and characterized. Besides greatly strengthened red absorption, the maximum solubility of compound 2 in phosphate buffered saline （PBS） is 4.2 mg/mL which is just enough to prepare an aqueous solution for intravenous injection in clinically acceptable concentration, while the partition coefficient between n-octanol and PBS, 5.6, benefits the cell-uptake and biological activity as well. Furthermore, EPR measurements reveal that the photosensitization activities of the two compounds to generate semiquinone anion radicals, superoxide anion radicals and singlet oxygen are a little bit higher than those of taurine substituted hypocrellin B （THB）, but the photodynamic activities to human lung cancer A549 cells are several times that of THB, mainly due to increases in lipophilicity and cell-uptake.     

小牛胸腺DNA的激光喇曼谱

用常规喇曼和表面增强喇曼的方法获得了小牛胸腺ＤＮＡ固体纤维和水溶液的激光喇曼谱，样品的喇曼特征谱带与国外文献及国内紫外共振喇曼法得到的谱图基本一致，由此可分析出样品的构象，基团归属等信息，证明喇曼光谱是用于测量生物分子结构的一个有力武器。     

竹红菌甲素和竹红菌乙素的光电转换性能

通过光电转换检测系统对竹红菌甲素和竹红菌乙素的光电转换性能进行了研究，结果表明两种光敏剂均具有良好的光敏性能，其中竹红菌甲素光敏电极无论是光电流还是光电压均要大于竹红菌乙素光敏电极，说明竹红菌甲素比竹红菌乙素的光敏性能更佳．     

Study on the binding mode of Mg（Sal2trien） with DNA

Polyamide could not only combine with DNA out- side but also inhibit expressing of gene[1]. At the same time, polyamide could coordinate many molecules. Ren et al.[2] found that [MgⅡ(dien)(OH)] can cleave pBR322DNA effectively in 2004. Liu et al.[3,4] f…     

镍在碱溶液中阳极氧化膜的拉曼光谱

利用拉曼光谱方法研究了Ｎｉ在１ｍｏｌ．Ｌ＾－１ＮａＯＨ溶液中阳极氧化膜的性质，结果表明，在电极电位为０．５５Ｖ时，表面氧化膜为β－ＮｉＯＯＨ，在０．３Ｖ和－０．４Ｖ时，虽然大部分β－ＮｉＯＯＨ已被还原成β－Ｎｉ（ＯＨ）２，但表面的最外层可能是ＮｉＯ薄层，在电位阶跃的同时，记录表面拉曼光谱光谱的变化，在β－ＮｉＯＯＨ膜的阴极还原时，光谱变化缓慢，可以认为，反应起始于β－ＮｉＯＯＨ／Ｎｉ界面或膜内部，     

光谱法研究PBBHAMF与小牛胸腺DNA的相互作用

以小牛胸腺DNA为研究对象,探讨了4’-苯基-3-溴-8-[N,N-二(2-羟基乙基)氨基甲基]黄酮(PB-BHAMF)与小牛胸腺DNA之间的相互作用模式.在以吖啶橙(AO)为荧光探针的实验中,DNA-AO复合物的荧光被PBBHAMF猝灭,其猝灭过程主要为静态猝灭;DNA的存在使PBBHAMF的紫外光谱发生了减色效应,DNA的粘度增大,CD光谱274 nm处信号发生变化,这些都能判断PBBHAMF与DNA发生了嵌插结合.此外,红外光谱结果表明PBBHAMF与DNA还存在静电结合.     

Mechanical behavior study of microdevice and nanomaterials by Raman spectroscopy: a review

With the rapid development of micro/nano manufacturing technology and nanomaterials, the accurate measurement of the mechanical properties and behaviors at the micronano scale represents a new field of mechanical experiments. Raman spectroscopy, which is based on lat tice dynamics theory, is applicable to the detection of the statistical information of the lattice structure deformation within the measuring points. Due to its peculiarities, such as non-destructiveness, convenience and highresolution, this technology allows the online in situ measurement of residual stress in microstructures caused by processing and can also achieve the realtime deformation of graphene, carbon nanotubes and other nanomaterials under force loading. In recent years, mechanical measurements based on Raman spectroscopy technology have developed rapidly. In this review, Ramanbased stress measurement theories for several commonly used materials are briefly described. Applications related to the residual stress measurements of microstructure and experimental investigations of the mechanical properties of lowdimensional nanomaterials are then reviewed. Finally, the development trend of this method is proposed.     

大豆球蛋白的红外和Raman光谱分析

利用Fourier变换红外光谱(FTIR)和激光Raman光谱研究大豆球蛋白的结构, 并对大豆球蛋白的红外光谱和Raman光谱的特征峰进行指认, 计算Raman费米共振\%I\%850/830的比值. 结果表明: 红外光谱和Raman光谱的酰胺Ⅰ带去卷积和曲线拟合获得二级结构, 其中β 折叠结果差异较小, α 螺旋、 β 转角和
无规卷曲结果差异较大（p<0.05）; 红外光谱在1 618,1 682 cm^-1处的吸收峰归属于大豆球蛋白的分子间和分子内聚集, 拟合峰面积百分数分别为11.1%和9.5%; 包埋和外露的酪氨酸残基占酪氨酸残基总量的14%和86%.     

毫米波辐照对昆明鼠皮肤组织Raman光谱的影响

为探讨高功率毫米波辐照对小鼠皮肤全部换为Raman光谱的影响,并为毫米波辐照的治疗和防护提供剂量学依据。采用高功率密度毫米波辐照小鼠背部,50s后立即取辐照部位皮肤制成样品,辐照样品和对照组样品冰冻切片后,测定毫米波辐照区域不同深度的小鼠皮肤组织切片的拉曼光谱。结果显示:与对照组比较,辐照后的特征峰位855cm-1、932cm-1消失,1080cm-1、1125cm-1和1166cm-1的峰强增大,1230~1410cm-1峰带中峰位增多,1585cm-1和1653cm-1峰强度大幅增加,2722cm-1峰减小及2930cm-1峰左侧翼减小。这表明,毫米波辐照50s使得小鼠皮肤组织中的蛋白质构象发生了变性,皮肤组织受到一定程度的损伤。     

与DNA键联的激发态Cu（NACN）共振拉曼研究

用４００ｎｍ和４４５ｎｍ脉冲激光激发测量水溶铜卟啉（４－Ｎ－乙腈基－吡啶基铜卟啉）及其与小牛胸腺ＤＮＡ复合物的共振拉曼光谱。分析表明Ｃｕ（ＮＡＣＮ）与ＤＮＡ形成了电子激发态复合物。观察到除铜卟啉的谱带６和８外，７带也在它的低频区出现了代表形成激发态复合物的额外峰。激发态复合中铜卟啉骨架发生了较大畸变，而吡啶取代基团没有明显变化，并且激发态复合中卟啉核π电子与ＤＮＡπ电子之间产生了比基态时强得多的相互作用。     

甲醇和乙醇的激光喇曼光谱

采用激光喇曼方法,对甲醇和乙醇液体样品进行了喇曼光谱研究,得到了上述样品的喇曼光谱,并对其进行了归属.经过分析比较,给出了检测甲醇的特征谱线,为假酒鉴别、深入研究甲醇和乙醇的能级结构等提供了可供借鉴的参考.     

Raman spectroscopic studies on the structural changes of poly(3-methylthiophene) during heating and cooling processes

Poly(3-methylthiophene) (PMeT) electrosynthesized by direct oxidation of 3-methylthiophene in boron trifluoride diethyl etherate (BFEE) has been studied by Raman spectroscopy in the temperature scale of 123-458 K. Experimental results demonstrate that the thermal stability of PMeT in the oxidized state is much lower than that of the polymer in the neutral state. Furthermore, during the cooling process, the conformation of neutral species changes from a coil-like state into a rod-like state, while the conformation of the oxidized species does not change.