几种番茄品种基因组DNA的相似指数初步分析

用４种随机引物（１０ｂｐ）,对普通番茄５个品种的基因组ＤＮＡ进行了ＰＣＲ扩增,并对其ＲＡＰＤ带谱进行了统计处理,发现不同随机引物扩增出来的ＲＡＰＤ标记所表现的相似指数有所不同,综合考虑,普通番茄种内不同品种同ＤＮＡ多态性保持了较高的稳定性。     

PCR扩增纳豆激酶基因的程序优化

研究了利用ＰＣＲ从纳豆菌基因组ＤＮＡ中扩增纳豆激酶基因的最优化条件，得到ＰＣＲ反应在本研究中的最重要的三个参数值为：模板量１０ｎｇ；Ｍｇ＾２＋浓度１．５ｍｍｏｌ／Ｌ；退火温度６０℃。在这种优化程序下进行ＰＣＲ反应，获得了特异、高效和忠实的纳豆激酶基因产物。     

热激下小白菜矮杂一号和矮脚黄呼吸作用及游离脯氨酸代谢

以抗热性不同的小白菜品种矮杂一号（抗热性品种）和矮脚黄（对照品种）为材料，测定了其呼吸强度，α－酮戊二酸脱氢酶（α－ＫＧＤＨａｓｅ）活性，游离脯氨酸（ｐｒｏ）含量和二氢吡啶－５－羧酸还原酶（Ｐ５ＣＲ）活性。实验结果表明，当热激温度为４０℃和４５℃时，矮杂一号呼吸强度持续稳定地上升，而矮脚黄则迅速下降。说明非抗热品种在高温下呼吸代谢不稳定，各个处理温度下，两个品种α－ＫＧＤＨａｓｅ活性有相同的变化趋     

中国鹅5个品种随机扩增多态DNA(RAPD)分析

目的 用随机扩增多态ＤＮＡ（ＲＡＰＤ）技术分析中国鹅的５个品种一皖西白鹅、太湖鹅、浙江白鹅、四川白鹅和狮头鹅的随机扩增多态ＤＮＡ（ＲＡＰＤ）。方法 从３４个１０－寡核苷酸随机引物中筛选出的１４个引物对每一品种的总基因组ＤＮＡ进行了单引物ＰＣＲ扩增,结果 ５个品种共扩增出１７４个ＤＮＡ片段,其中４８个（占２７．６％）在５个品种间表现多态性,根据扩增ＤＮＡ片段的异同计算了各品种间的遗传距离指皖西白鹅和     

葡萄属植物的基因组DNA提取及RAPD体系优化

采用ＣＴＡＢ改良法提取葡萄属７种植物的基因组ＤＮＡ,对其完整性进行了测定,并以此为模板进行ＲＡＰＤ反应,对ＲＡＰＤ反应体系的５个主要影响因子进行系统的初选与复选,获得了葡萄属植物的ＲＡＰＤ最优反应体系及扩增程序。     

西瓜种植物RAPD分析影响因子的研究

以西瓜种内的１２个品种（系）为模板ＤＮＡ,研究了影响ＲＡＰＤ扩增效果的因素。结果表明,模板ＤＮＡ、Ｔａｑ酶、随机引物、Ｍｇ２＋浓度以及ＰＣＲ反应程序在ＲＡＰＤ分析中都具有重要的作用。根据以上因子的研究结果,建立了适合西瓜种植物的ＲＡＰＤ分析的技术体系：１０μＬ反应液中,模板１０ｎｇ／１０μＬ,引物０５μｍｏｌ／Ｌ,ＴｒｉｓＨＣｌ（ｐＨ８３）５０ｍｍｏｌ／Ｌ,ＢＳＡ５００μｇ／ｍＬ,ＭｇＣｌ２２５ｍｍｏｌ／Ｌ,ＴａｑＤＮＡ０６Ｕ／１０μＬ,ｄＮＴＰｓ２００μｍｏｌ／Ｌ,１０％蔗糖４００和１ｍｍｏｌ／Ｌ甲酚红。     

日本脑炎病毒（JEV）内蒙古分离株M73—1E基因的5′端？…

根据国外报道的ＪＥＶ（Ｊａｐａｎｅｓｅ ｅｎｃｅｐｈａｌｉｔｉｓ ｖｉｒｕｓ）基因组全序列,针对ＪＥＶ Ｅ基因５′端设计合成一对特异引物,以日本脑炎病毒内蒙古分离株Ｍ７３－１ＲＮＡ为模板,经ＲＴ－ＰＣＲ扩增,获得３６６ｂｐ的ＪＥＶ Ｅ基因ｃＤＮＡ片段。将此片段重组于质粒ｐＵＣ１９中,并转化大肠杆菌ＤＨ５α,经ＰＣＲ扩增,酶切及序列分析,结果表明ＪＥＶ Ｍ７３－１ ５′端１２６个核苷酸序列与ＪＥＶ日     

经典Ramsey数R(7,n)的5个下界

通过计算机构造了５个完全图的新的循环图分解,从而获得了Ｒａｍｓｅｙ数Ｒ（７,１８）,Ｒ（７,１９）,Ｒ（７,２０）,Ｒ（７,２１）和Ｒ（７,２２）的下界．这５个结果填补了Ｒａｍｓｅｙ数研究的５个空白．     

荔枝LitchiChinensisSonn果皮热处理护色技术的探讨

选择妃子笑,糯米糍,淮枝等广东省主栽荔枝品种,比较研究了不同热处理技术包括热水（及沸水）,热空气,热蒸汽及其实施配合浸酸处理对荔枝皮颜色保持的效果。结果表明：果实分别经９８℃沸水３ｓ,５６℃热水５ｍｉｎ,高温条件下９５℃,热空气５ｍｉｎ,或９５℃,热蒸汽３－５ｓ处理后再在ｐＨ０．６的ＨＣｌ溶液中浸泡５ｍｉｎ,或９５℃,热蒸汽３－５ｓ处理后再在ｐＨ０．５的ＨＣｌ溶液中浸泡５ｍｉｎ,能有效提高并稳定保     

5个新的素数阶循环图

通过计算机构造了５个新的素数阶循环图,从而获得了Ｒａｍｓｅｙ数的５个下界：Ｒ（６,１３）≥２４２,Ｒ（８,１７）≥６０２,Ｒ（８,１８）≥６４２,Ｒ（８,１９）≥６８４,Ｒ（８,２０）≥７６２．这些结果填补了Ｒａｍｓｅｙ数研究的５个空白．     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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