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1.
Summary The rate of development of Ruhemann's purple in the ninhydrin reaction of two deuterated primary amines, -d2-p-tyramine and -d2--phenylethylamine, is significantly reduced It appears to be a primary isotope effect and indicates that the cleavage of the carbon-hydrogen bond at the -position is involved in the rate-determining step of the color reaction.Acknowledgements. We thank Dr A.A. Boulton for his advice and encouragement and the Canadian Medical Research Councel and Saskatchewan Health for their continuing financial support. 相似文献
2.
I. A. Macdonald 《Cellular and molecular life sciences : CMLS》1978,34(4):426-427
Summary Tritium distribution on randomly labelled taurocholate (TC) was estimated at 28%, 4%, 1% and <0.5% on the hydrogens opposite the 3-, 7- and 12-OH groups and taurine moiety respectively. Anomalously,C. perfringens 3-hydroxysteroid dehydrogenase (3-HSDH) catalyzed tritium loss of 36% on formation of 7-, 12-dihydroxy-3-keto-5-cholanoate, implying additional losses of tritium at other sites by this enzyme.This work is supported by the National Cancer Institute. 相似文献
3.
O. E. Tsitsiloni P. P. Yialouris H. Echner W. Voelter A. A. Haritos 《Cellular and molecular life sciences : CMLS》1992,48(4):398-402
A new radioimmunoassay has been developed for thymosin 4 by generating rabbit polyclonal antibodies against the synthetic N-terminal peptide fragment 1–15 coupled to KLH. The synthetic analogue [Tyr12]-thymosin 4 (1–15) was used as tracer. This radioimmunoassay, with a useful range of 10–1000 pmoles, showed cross-reactivity with the second homologous -thymosin of man and rat (thymosin 10) but not of calf (thymosin 9). This radioimmunoassay, together with an improved radioimmunoassay for the N-terminus of parathymosin , was employed for the measurement of the levels of thymosin 4 and parathymosin in nuclear and extranuclear extracts of calf thymus. The bulk of these polypeptides was found in the extranuclear material whereas only traces were observed in the nuclear environment, which indicates the extranuclear localisation of - and -thymosins. 相似文献
4.
J. Brown M. L. Forsling G. Valdes J. D. H. Slater C. T. Dollery 《Cellular and molecular life sciences : CMLS》1988,44(6):513-516
Summary Human -atrial natriuretic peptide (h-ANP) makes the urine of dehydrated volunteers hypotonic to plasma despite high circulating concentrations of antidiuretic hormone. Urinary dilution with h-ANP also occurs in subjects receiving indomethacin. Therefore, h-ANP antagonises effects of antidiuretic hormone on distal tubular V2-receptors in man, probably without involving prostaglandins. 相似文献
5.
M. E. Charness 《Cellular and molecular life sciences : CMLS》1989,45(5):418-428
Summary Ethanol may modulate endogenous opioid systems by disrupting opioid receptor signalling. Low concentrations of ethanol slightly potentiate -opioid receptor binding by increasing receptor Bmax, and, in some cases, chronic ethanol exposure decreases the density or affinity of the -opioid receptors. By contrast, high concentrations of ethanol acutely decrease -opioid receptor binding by decreasing receptor affinity, whereas chronic exposure of animals and neuronal cell lines to lower concentrations of ethanol leads to possibly adaptive increases in the density or affinity of the -opioid receptors. In the neuronal cell line NG108-15, ethanol does not up-regulate the -opioid receptor by blocking receptor degradation or endocytosis, but protein synthesis is required for this response. Up-regulation of the -opioid receptor renders ethanol-treated NG108-15 cells 3.5-fold more sensitive to opioid inhibition of adenylyl cyclase. Long-term treatment with ethanol also increases maximal opioid inhibition in NG108-15 cells, possibly by decreasing levels of Gs and its mRNA. Ethanol differentially modulates signal transduction proteins in three additional neuronal cell lines, N18TG2, N4TG1, and N1E-115. Ethanol-treated N18TG2 cells show the least up-regulation of the -opioid receptor, little heterologous desensitization of adenylyl cyclase, and no changes in Gs or Gi. By contrast, ethanol-treated N1E-115 cells show the largest up-regulation of the -opioid receptor, the most heterologous desensitization of adenylyl cyclase, and concentration-dependent decreases in Gs and increases in Gi. Further analysis of these related neuronal cell lines may help to identify the molecular elements that endow some, but not all, neuronal cells with the capacity to adapt to ethanol. 相似文献
6.
G. W. Oertel P. Knapstein K. Heide 《Cellular and molecular life sciences : CMLS》1969,25(11):1192-1193
Zusammenfassung Nach. i.v. Gabe von 7-3H-Dehydroepiandrosteron wurde menschliches Plasma einer präparativen Zonenelektrophorese unterworfen und in Albumine,
1-,
2-,- und-Globuline zerlegt. Aus den einzelnen Proteinfraktionen trennte man freie Steroide, Steroid-Sulfokonjugate und -Glukuronoside ab und untersuchte letztere Fraktionen auf ihren Gehalt an markierten C19-Steroiden. Es zeigte sich, dass die Steroid-Sulfokonjugate vornehmlich in der Proteinfraktion der Albumine, die Steroid-Glukuronoside dagegen bevorzugt in der Proteinfraktion der
1-Globuline auftraten. 相似文献
7.
Summary 3, 16-diacetoxy-5-pregnane-20-one and the analogue pregn-5-ene derivative have been converted into (22R, 25R)-22, 26-imino-5-cholestane-3, 16-diol. The configuration at C-22 and C-25 was determined by transformation into the known (22R, 25R)-5-solanidane-3-one. Application of the Ruschig method led to (25R)-22,26-imino-5-cholest-22(N)-ene-3, 16-diol.
Solanum-Alkaloide. XVI. Mitteilung. — XV. Mitteilung:K. Schreiber undH. Ripperger, Tetrahedron Letters Nr.27, 9 (1960). 相似文献
Solanum-Alkaloide. XVI. Mitteilung. — XV. Mitteilung:K. Schreiber undH. Ripperger, Tetrahedron Letters Nr.27, 9 (1960). 相似文献
8.
P. -C. Thiercelin B. Quintard J. Saint-Blancard 《Cellular and molecular life sciences : CMLS》1966,22(4):226-228
Summary In vitro, the mixture Chlorpromazine-human serum gives modifications of the lipoproteins and the-globulins. These modifications are studied by paper electrophoresis, Tiselius-electrophoresis, and immuno-electrophoresis. Under the experimental conditions described, the selective precipitation of the
1-globulins seems quite unexpected. 相似文献
9.
Summary The effects of selected -agonists and -antagonists on theophylline-induced lipolysis were investigate in isolated hamster white fat cells 2-Agonists (tramazoline, clonidine) inhibited theophylline-induced lipolysis while an 2-agonist (methoxamine) was without any effect. The inhibitory effect of 2-agonists was suppressed by yohimbine (2-antagonist), whereas 2-antagonists were inefficient. This result implies that the -adrenergic receptor of hamster fat cells is of the 2-type, although located postsynaptically.Acknowledgments. This work was supported by grants from CNRS (ERA 412) and DGRST (grant No. 787 1078). We thank M. Dauzats for excellent technical assistance. We thank Prof. H. Schmitt for tramazoline and AR-C 239 and for helpful discussion. 相似文献
10.
Summary Starting from 17-hydroxy-17-hydroxymethyl-4-androsten-3-one, we synthesized 4-chloro-17-hydroxy-17-hydroxymethyl-4-androsten-3-one-17, 20-acetonide and 17-hydroxy-17-hydroxymethyl-1, 4-androstadien-3-one. 11, 17-dihydroxy-17-hydroxymethyl-4-androsten-3-one was obtained from cortisone via methyl-17-hydroxy-3, 11-dioxo-4-etienate. 相似文献