红细胞生成素(EPO)在中国仓鼠卵巢细胞(CHO)中稳定表达

将ＥＰＯｃＤＮＡ分别插入真核表达质粒载体ｐＳＶ２ｄｈｆｒ和ｐｃＤＮＡ３的适当位点,用电脉冲法导入ＣＨＯ细胞,用ＥＬＩＳＡ法检测ＥＰＯ的表达量,用ＴＦ１细胞检测ＥＰＯ表达产物的生物活性．结果表明,用ｐＳＶ２ｄｈｆｒ作为载体,在抗ＭＴＸ阳性细胞克隆中,获得了表达ＥＰＯ（２５８ｎｇ／ｍＬ培养液）的ＣＨＯ细胞株,并且表达产物具有生物活性．进一步的传代和冻存试验表明,所得细胞株表达ＥＰＯ的水平不受传代和冻存的影响     

人肿瘤细胞株中细小病毒H—1的DNA扩增与非结构蛋白NS—1基因的表达

研究三株人癌细胞和两株对照细胞对细小病毒Ｈ－１杀伤作用敏感性的分子机制．表明了在感染复数ｍｏｉ（ｍｕｌｔｉｐｉｃｉｔｙｏｆｉｎｆｅｃｔｉｏｎ）为５ｐｆｕ（ｐｌａｑｕｅｆｏｒｍｉｎｇｕｎｉｔ）／细胞的情况下，作为Ｈ—１病毒复制受纳细胞的人肝癌细胞株ＯＧＹ－７７０３和人胃癌细胞株ＳＧＣ—７９０１，能够支持病毒ＤＮＡ扩增和非结构蛋白ＮＳ—１基因的表达，这和作为阳性对照的由ＳＶ４０转化的新生儿肾细胞株ＮＢ—Ｋ一样，但对Ｈ—１病毒感染有抗性的人肾癌细胞株ＯＵＲ—１０和它的对照人胎肾细胞株ＨｕＫ—１，并不支持病毒ＤＮＡ扩增和ＮＳ—１蛋白的表达．本文结果指出，细小病毒Ｈ—１的杀伤作用与细胞中的病毒ＤＮＡ扩增及ＮＳ—１基因表达的程度相关．     

用复制酶基因cDNA探针检测表达病毒外壳蛋白基因马…

利用马铃薯卷叶病毒（ＰＬＲＶ）复制酶基因３＇端长约６００ｂｐ的ｃＤＮＡ，用缺口平移方法进行＾３２Ｐ同位素标记，制备成ｃＤＮＡ探针，通过核酸斑点杂交，对提纯的马铃薯卷叶病毒（ＰＬＲＶ）、病毒ＲＮＡ、ＰＬＲＶ感染的马铃薯汁液，表达ＰＬＲＶ ＣＰ基因的马铃薯叶片及块茎芽进行了检测。结果表明，＾３２Ｐ标记的复制酶基因ｃＤＮＡ探针特异性强、灵敏度高。可测得提纯病毒的最低含量为４０８ｎｇ／ｍｌ，病毒ＲＮＡ最低     

鸭乙肝病毒表面抗原特异性单抗的研制及应用

以纯化的鸭乙型肝炎病毒表面抗原（ＤＨＢｓＡｇ）免疫ＢＡＬＢ／ｃ小鼠,取免疫鼠脾细胞与小鼠骨髓瘤ＳＰ２／Ｏ－Ａｇ１４融合,应用ＥＬＩＳＡ筛选出５Ｂ８和８Ｇ１１ ２株抗体分泌滴度较高的杂交瘤细胞,二者所分泌的单抗仅与ＤＨＢｓＡｇ发生反应,而与其它９种禽类常见的病毒均不发生反应,其亚类鉴定分别属于ＩｇＭ和ＩｇＧ２ａ。用鸭乙型肝炎病毒（ＤＨＢＶ）阳性鸭血清中粗提的病毒悬液进行５Ｂ８株单抗介导的ＳＰＡ胶体金     

RP—HPLC法测定葛根芩连胶囊中盐酸小檗碱的含量

用ＲＰ－ＨＰＬＣ法测定葛根芩连胶囊中盐酸小檗碱的含量。采用Ｓｈｉｍａｄｚｕ ＶＰ－ＯＤＳ柱，乙腈－０．０６ｍｏｌ／Ｌ ＫＨ２ＰＯ４－ＳＤＳ（２５０：３００：３．３ｇ）为流动相，检测波长３４７ｎｍ。方法准确可靠，快捷方便，回收率为９９．６０％，ＲＳＤ为１．０６％。     

腺病毒同源重组子的鉴定

携带入ＩＦＮ－γｃＤＮＡ序列的Ｅ１区缺失的腺病毒载体ｐＡｄｌ２／ＲＳＶ－ＩＦＮ－ｂｐＡ与ｐＪＭ１７质粒，通过磷酸钙沉淀法共转染２９３细胞，经同源重组，共获得６个病毒噬斑。病毒噬斑感染２９３细胞，至出现完全细胞病变效应（ＣＰＥ）后，抽提细胞内的病毒ＤＮＡ，经ＸｈｏⅠ酶切后走凝胶电泳，出现重组腺病毒圾的３．５ｋｂ大小的ＤＮＡ条带，＾３２Ｐ标记的探针ｐＡｄｌ２／ＲＳＶ－ＩＦＮ－ｂｐＡ与ＸｈｏⅠ酶切过的病     

用复制酶基因cDNA探针检测表达病毒外壳蛋白基因马铃薯中的PLRV

利用马铃薯卷叶病毒（ＰＬＲＶ）复制酶基因３′端长约６００ｂｐ的ｃＤＮＡ，用缺口平移方法进行３２Ｐ同位素标记，制备成ｃＤＮＡ探针，通过核酸斑点杂交，对提纯的马铃薯卷叶病毒（ＰＬＲＶ）、病毒ＲＮＡ、ＰＬＲＶ感染的马铃薯汁液，表达ＰＬＲＶＣＰ基因的马铃薯叶片及块茎芽进行了检测．结果表明，３２Ｐ标记的复制酶基因ｃＤＮＡ探针特异性强、灵敏度高．可测得提纯病毒的最低含量为４０８ｎｇ／ｍｌ，病毒ＲＮＡ最低量为２７．８ｐｇ／ｍｌ，未转ＣＰ基因接种ＰＬＲＶ的马铃薯叶片提取液的最高稀释度为１３７５．接种ＰＬＲＶ的转ＣＰ基因的抗性马铃薯块茎芽和叶片提取液的最高稀释度为０～１／１５．此探针和只转入病毒外壳蛋白基因，不接种ＰＬＲＶ的转基因马铃薯汁液不发生反应．表明，探针只与ＰＬＲＶ基因组ＲＮＡ特异反应，而不与外壳蛋白基因及其ｍＲＮＡ反应，从而为表达ＣＰ基因的转基因马铃薯中ＰＬＲＶ的检测和抗病性鉴定建立了一种可靠的灵敏的分子生物学技术．此项技术已用于转ＣＰ基因马铃薯Ｄｅｓｉｒｅ，Ｆａｖｏｒｉｔａ，乌盟６０１和虎头的抗病性鉴定     

化学沉积Ni-Cu-P合金及晶化处理对镀层结构和硬度的影响

从弱碱性化学镀溶液中化学沉积Ｎｉ－Ｃｕ－Ｐ合金,并用差热分析（ＤＳＣ）、Ｘ－射线衍射（ＸＲＤ）和透射电镜（ＴＥＭ）研究了晶化处理对镀层结构及硬度的影响。结果表明,镀液中ＣｕＳＯ４．５Ｈ２Ｏ浓度对合金组成有显著影响。在化学沉积过程中,Ｃｕ的优先析出使镀层中Ｃｕ／Ｎｉ摩尔比远远高于镀液中Ｃｕ＾２＋／Ｎ＾２＋摩尔比。镀液中ＳｕＳＯ４．５Ｈ２Ｏ浓度低时,沉积速度随其浓度增加而增大,但ＣｕＳＯ４．５Ｈ２Ｏ浓     

重组人钙调素的表达及其McAb的制备与精子CaM定位

用基因重组技术构建人钙调素基因Ⅲ（ｈＣａＭⅢｃＤＮＡ）表达载体ｐＢＶ／ｈＣａＭⅢ,并在大肠杆菌ＤＨ５α中经热诱导获得可溶性ＣａＭ蛋白的高效表达．将纯化的重组ｈＣａＭ与异型双功能剂ＳＰＤＰ及鼠血清白蛋白（ＭＳＡ）交联,免疫Ｂａｌｂ／ｃ小鼠,用常规细胞融合技术制备单克隆抗体（ＭｃＡｂ）,得到３株稳定分泌单克隆抗体的杂交瘤细胞．间接ＥＬＩＳＡ和斑点免疫结果证实,三种单克隆抗体均与自制的ｒｈＣａＭ和ＣａＭ标准品起特异反应．用免疫组化法对精子中ＣａＭ定位,发现ＣａＭ主要分布于精子头颈部,不育组ＣａＭ＋精子率（（４５．０±７．５）％）显著低于生育组（（６８．５±１０．５）％）．     

hSOD基因在大白鼠肺上皮细胞中的表达

本文以哺乳动物细胞表达载体ｐＲｃ／ＣＭＶ为运载体,构建了受控于ＣＭＶ启动子的ｈＳＯＤ基因的表达载体ｐＲｃ／ＣＭＶ＿ＳＯＤ,用脂质体转染法转染大白鼠肺上皮细胞Ｌ２细胞后,经抗生素Ｇ４１８选择性培养,获得与细胞染色体ＤＮＡ稳定整合的ｈＳＯＤｃＤＮＡ导入细胞,经ＳｏｕｔｈｅｒｎＢｌｏｔｓ和ＷｅｓｔｅｒｎＢｌｏｔｓ分析,结果表明,ｈＳＯＤ在细胞中得到稳定表达,同时测定细胞ＳＯＤ酶活性,ｈＳＯＤｃＤＮＡ导入细胞的ＳＯＤ酶活性是空载体导入细胞和父本细胞的约２倍,并且在选择细胞克隆之后的６０天内是持续的．     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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