Regulation of feeding and ovipositional success ofAmblyomma americanum ticks

Summary Duration of the adult femaleAmblyomma americanum feeding period was found to be dependent upon male receptiveness to mating, which was in turn dependent upon when males were placed with females. Prolonging the time to mating by 5 days had no effect on female engorgement weight or length of the preovipositional period, but prolonging mating by 10 days substantially decreased the number of ovipositing females and ablated egg viability. In the absence of males, females were severely stunted in size and had to be forcibly removed from the host.Supported in part by grants from the United States Public Health Service, National Institutes of Health No. AI 17555, RR 05443, and from the Rockefeller Foundation.     

The composition of Taft E s o constants: An application of biased alternatives to least squares

Summary A systematic analysis of the composition of E _s ^o like rate constants clearly revealed that Taft E _s ^o -values depend upon the size of the substituents. Further evidence in favor of this view is adduced even in a case where OLS led to the conclusion that E _s ^o should be completely independent of the size of the substituents, since biased estimators (PCRA, LRRA) showed that this statement is not correct. Furthermore, it seems that the magnitude of the steric effect represented by E _s ^o is a function of the thickness of the substituent along 2 directions perpendicular on its main axis and is not influenced by its length.Acknowledgment. We would like to thank Prof. J.T. Webster, Department of Statistics, Southern Methodist University, Dallas, for sending us s computer program of latent root regression analysis. We also thank the referees whose insightful comments were most helpful in improving the presentation.     

Effect of adaptation level on maintained firing and sensitivity in receptive field center of X and Y cells

Maintained firing rates of X cells and Y cells were compared at 6 adaptation levels (AL) between -2.71 log cd/m2 and 2.28 log cd/m2 (10 mm2 pupil size). X cell maintained firing was higher at all ALs and was statistically different at medium and high ones. Changes in AL had nearly identical effects upon X and Y cell suprathreshold sensitivity to a flashing spot in the center of their receptive fields; the Weber function had a slope of 0.744 for Y cells and 0.743 for X cells. These values are not statistically different.     

Eiweissvermehrung in ein- und zweikernigen systemen vonAcetabularia

Summary (1) The rate of protein synthesis was found to be different inAcetabularia crenulata andAcetabularia mediterranea the higher cytoplasmic protein synthesis inA. crenulata depending upon the diameter of the stalk.(2) In systems containing one or two nuclei, there was no difference in the rate of cytoplasmic synthesis of proteins. This corresponds to the diminution of size and efficiency of the nuclei in binucleated systems.(3) In interspecific grafts, the rate of cytoplasmic protein synthesis corresponds nearly to the rate of protein synthesis ofAcetabularia crenulata. Corresponding to morphogenetic processes, thecren-action is prevalent.     

Effect of adaptation level on maintained firing and sensitivity in receptive field center of X and Y cells

Summary Maintained firing rates of X cells and Y cells were compared at 6 adaptation levels (AL) between –2.71 log cd/m² and 2.28 log cd/m² (10 mm² pupil size). X cell maintained firing was higher at all ALs and was statistically different at medium and high ones. Changes in AL had nearly identical effects upon X and Y cell suprathreshold sensitivity to a flashing spot in the center of their receptive fields; the Weber function had a slope of 0.744 for Y cells and 0.743 for X cells. These values are not statistically different.This research is supported by Public Health Service grant no. EY 00701.     

The unconventional secretion of stress-inducible protein 1 by a heterogeneous population of extracellular vesicles

The co-chaperone stress-inducible protein 1 (STI1) is released by astrocytes, and has important neurotrophic properties upon binding to prion protein (PrP^C). However, STI1 lacks a signal peptide and pharmacological approaches pointed that it does not follow a classical secretion mechanism. Ultracentrifugation, size exclusion chromatography, electron microscopy, vesicle labeling, and particle tracking analysis were used to identify three major types of extracellular vesicles (EVs) released from astrocytes with sizes ranging from 20–50, 100–200, and 300–400 nm. These EVs carry STI1 and present many exosomal markers, even though only a subpopulation had the typical exosomal morphology. The only protein, from those evaluated here, present exclusively in vesicles that have exosomal morphology was PrP^C. STI1 partially co-localized with Rab5 and Rab7 in endosomal compartments, and a dominant-negative for vacuolar protein sorting 4A (VPS4A), required for formation of multivesicular bodies (MVBs), impaired EV and STI1 release. Flow cytometry and PK digestion demonstrated that STI1 localized to the outer leaflet of EVs, and its association with EVs greatly increased STI1 activity upon PrP^C-dependent neuronal signaling. These results indicate that astrocytes secrete a diverse population of EVs derived from MVBs that contain STI1 and suggest that the interaction between EVs and neuronal surface components enhances STI1–PrP^C signaling.     

Judgmental extrapolation and the salience of change

People may often forecast using cognitive procedures that resemble formal time-series extrapolation models. A model of judgmental extrapolation based on exponential smoothing is proposed in which the setting of the trend parameter is hypothesized to depend upon the relative salience of the successive changes. The salience hypothesis was first tested with exponential series by the use of a framing manipulation. As predicted, focusing the subjects' attention on the changes led to more accurate forecasts. In two investment simulation studies, the salience hypothesis was further examined by varying the statistical properties of the price changes. As predicted, subjects were more likely to sell as prices fell and to buy as prices rose (1) as the sample size of similar changes increased; (2) when the variance of the changes was low; and (3) when the absolute value of the mean change was high. Conditions that may influence judgmental forecasting processes are discussed.     

Activated scramblase and inhibited aminophospholipid translocase cause phosphatidylserine exposure in a distinct platelet fraction

Platelet procoagulant activity is mainly determined by the extent of surface-exposed phosphatidylserine (PS), controlled by the activity of aminophospholipid translocase and phospholipid scramblase. Here, we studied both transport activities in single platelets upon stimulation with various agonists. Besides the formation of procoagulant microparticles, the results show that a distinct fraction of the platelets exposes PS when stimulated. The extent of PS exposure in these platelet fractions was similar to that in platelets challenged with Ca²⁺-ionophore, where all cells exhibit maximal attainable PS exposure. The size of the PS-exposing fraction depends on the agonist and is proportional to the platelet procoagulant activity. Scramblase activity was observed only in the PS-exposing platelet fraction, whereas translocase activity was exclusively detectable in the fraction that did not expose PS. We conclude that, irrespective of the agonist, procoagulant platelets exhibit maximal surface exposure of PS by switching on scramblase and inhibiting translocase activity.Received 8 March 2005; received after revision 19 April 2005; accepted 13 May 2005     

Neuroglobin,seven years after

Neuroglobin is expressed in vertebrates brain and belongs to a branch of the globin family that diverged early in evolution. Sequence conservation suggests a relevant role in the nervous system, with tight structural restraints. Experiments in vivo and in vitro showed increased hypoxic stress damage upon repressing neuroglobin biosynthesis and improved recovery following overexpression. Neuroglobin shows internal heme hexacoordination, which controls oxygen affinity and kinetics. Neuroglobin concentration, oxygen affinity and enhanced autooxidation question a role in oxygen delivery; thus it was proposed that the neuroprotective effect might be due to radical scavenging or activation of protection mechanisms. Neuroglobin's structure shows a peculiar internal cavity of very large size. Binding of heme ligands is associated to a conformational change involving the heme that "slides" into the pre-existing cavity and makes the sixth coordination position available. These features may pave the way to an understanding of neuroprotection by neuroglobin.     

Vergleichende Untersuchungen über die angeborene Grössenunterscheidung beiTilapia nilotica undHemihaplochromis multicolor (Pisces; Cichlidae)

Summary Female mouth-brooding Cichlid fishes pick up the young fry again when disturbed. The young, for their part, collect about the head of the mother fish and, contacting it, try to enter the mouth cavity. This contact behaviour is based upon an inborn readiness to react on the visual stimulus offered by the mother. Mother fishes inTilapia nilotica are much larger than those ofHemihaplochromis multicolor. By comparative experimental studies with models of various size (black balls of different diameters) it is shown that the young of the 2 species strictly prefer different sizes of the model, corresponding with the different sizes of the parent fishes.

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Herrn Prof.H. M. Peters zum 60. Geburtstag gewidmet.     

Das beim Gefrieren von Gelen entstehende Eis

Summary Highly swollen gels which contain, in addition to a large amount of solvent, a spatial network formed by chains of a high polymer, show an abnormal lowering of the freezing point. This is caused by the fact that the network prevents the formation of macroscopically undisturbed crystals.If, in the course of freezing at this lower temperature, a conglomerate of microcrystals were created corresponding in size to the width of the network, one should be able to observe a broadening of the X-ray interference lines. Experiments have shown that no measurable broadening takes place.This is explained by the assumption that a crystal which is prevented from growing in the normal way by the surrounding filaments of the gel, when cooled to a suitably low temperature grows crystallites with a small cross-section corresponding to the mesh size. The crystallites thus formed are orientated in a crystallographically exact manner toward their supporting crystal and parallel to each other. Therefore, when reaching the necessary height, they unite to larger, ordered crystalline regions which incorporate the polymeric filaments. This explains the observed sharpness of the X-ray interferences as well as the observation that, upon heating of a frozen gel, the melting does not occur at the same low temperature as the freezing, but closer to the melting point of macroscopic ice.     

Voltage-gated sodium channel-associated proteins and alternative mechanisms of inactivation and block

Voltage-gated sodium channels mediate inward current of action potentials upon membrane depolarization of excitable cells. The initial transient sodium current is restricted to milliseconds through three distinct channel-inactivating and blocking mechanisms. All pore-forming alpha subunits of sodium channels possess structural elements mediating fast inactivation upon depolarization and recovery within milliseconds upon membrane repolarization. Accessory subunits modulate fast inactivation dynamics, but these proteins can also limit current by contributing distinct inactivation and blocking particles. A-type isoforms of fibroblast growth factor homologous factors (FHFs) bear a particle that induces long-term channel inactivation, while sodium channel subunit Navβ4 employs a blocking particle that rapidly dissociates upon membrane repolarization to generate resurgent current. Despite their different physiological functions, the FHF and Navβ4 particles have similarity in amino acid composition and mechanisms for docking within sodium channels. The three competing channel-inactivating and blocking processes functionally interact to regulate a neuron’s intrinsic excitability.     

Propriétés phytobiologiques de la sérotonine

Summary The serotonin (5-hydroxytryptamin) which shows a slight influence upon the growth of plant tissues, acts upon water uptake by regulating cellular permeability (latter is activated in the case of K⁺).     

Nucleolar localization and circadian regulation of Per2S,a novel splicing variant of the Period 2 gene

In this work, we show for the first time that a second splicing variant of the core clock gene Period 2 (Per2), Per2S, is expressed at both the mRNA and protein levels in human keratinocytes and that it localizes in the nucleoli. Moreover, we show that a reversible perturbation of the nucleolar structure acts as a resetting stimulus for the cellular clock. Per2S expression and periodic oscillation upon dexamethasone treatment were assessed by qRT-PCR using specific primers. Western blot (WB) analysis using an antibody against the recombinant human PER2 (abRc) displayed an intense band at a molecular weight of ~55 kDa, close to the predicted size of Per2S, and a weaker band at the expected size of Per2 (~140 kDa). The antibody raised against PER2 pS662 (abS662), an epitope absent in PER2S, detected only the higher band. Immunolocalization studies with abRc revealed a peculiar nucleolar signal colocalizing with the nucleolar marker nucleophosmin, whereas with abS662 the signal was predominantly diffuse all over the nucleus and partially colocalized with abRc in the nucleolus. The analysis of cell fractions by WB confirmed the enrichment of PER2S and the presence of PER2 in the nucleolar compartment. Finally, a pulse (1 h) of actinomycin D (0.01 μg/ml) induced reversible nucleolar disruption, PER2S de-localization and circadian synchronization of clock and Per2S genes. Our work represents the first evidence that the Per2S splicing isoform is a clock component expressed in human cells localizing in the nucleolus. These results suggest a critical role for the nucleolus in the process of circadian synchronization in human keratinocytes.     

Science and the romantic movement

Several authors have used the expression ‘formal asymmetry’ to characterize Einstein's method of introducing conceptual innovations. Prior to his use of formal asymmetries, however, Einstein relied upon analogy to introduce his major concepts, but without satisfactory results. He gradually refined another technique, reflection upon empirical problems, into the method of formal asymmetries, with impressive results. This historical study, based upon a textual analysis of Einstein's publications, raises a series of questions regarding the place of formal asymmetries in his work.     

Cytological effect of some medicinal plants used in the control of fertility

Summary The effects induced upon the cell cycle of Allium cepa meristems by 2 medicinal plants used in the control of fertility were studied. Infusions of Aristolochia triangularis induces typical c-mitotic figures. On the other hand, Stevia rebaudiana have no specific toxicological effects upon the cell cycle.This work was supported by a research grant from the Instituto para el Estudio de la Reproducción Humana, Asunción (Paraguay).     

Action du LiCl sur de jeunes blastodermes de poulet cultivésin vitro

Summary The young blastoderms cultivatedin vitro, by the method ofNew, slightly modified, grow abnormally on a medium of albumen mixed with LiCl solution. It acts in the first place upon the morphogenesis, secondarily upon the differentiation of organs, if they are able to develop.

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Travail subventionné par le Fonds national suisse de la Recherche scientifique.     

Structural and functional properties of porin channels in E. coli outer membranes.

Porin is a channel-forming, voltage-dependent protein of E. coli outer membranes. It exhibits relatively unspecific molecular sieve properties (exclusion size 600 Da). The trimer (110 kDa) consists of three identical polypeptides. Its secondary structure is mostly beta-structure, part of which can be visualized by electron microscopy to form a single beta-pleated sheet near the protein-lipid interface of the trimer. This folding pattern is significantly different from those of the reaction centers and of bacteriorhodopsin. Moreover, it contains many polar and ionizable side chains. It is argued that local as well as global electroneutrality, and complete saturation of the entire hydrogen bonding potential not only allow the protein to reside in the hydrophobic membrane core, but also confer upon it its unusual stability.     

Acute cold exposure increases the glucagon sensitivity of thermogenic metabolism in the rat

Summary Administration of glucagon to rats at 25°C had no effect upon their VO₂ while administration of noradrenaline or noradrenaline plus glucagon raised the VO₂-At 5°C, noradrenaline had no effect upon the cold-enhanced VO₂, while glucagon caused a rise of 13.7% implying increased glucagon sensitivity at 5°C. The glucagon-induced enhancement of VO₂ was abolished by concurrent administration of noradrenaline.     

Sonic hedgehog signaling pathway in vertebrate epithelial appendage morphogenesis: perspectives in development and evolution

Vertebrate epithelial appendages are elaborate topological transformations of flat epithelia into complex organs that either protrude out of external (integument) and internal (oral cavity, gut) epithelia, or invaginate into the surrounding mesenchyme. Although they have specific structures and diverse functions, most epithelial appendages share similar developmental stages, including induction, morphogenesis, differentiation and cycling. The roles of the SHH pathway are analyzed in exemplary organs including feather, hair, tooth, tongue papilla, lung and foregut. SHH is not essential for induction and differentiation, but is involved heavily in morphogenetic processes including cell proliferation (size regulation), branching morphogenesis, mesenchymal condensation, fate determination (segmentation), polarizing activities and so on. Through differential activation of these processes by SHH in a spatiotemporal-specific fashion, organs of different shape and size are laid down. During evolution, new links of developmental pathways may occur and novel forms of epithelial appendages may emerge, upon which evolutionary selections can act. Sites of major variations have progressed from the body plan to the limb plan to the epithelial appendage plan. With its powerful morphogenetic activities, the SHH pathway would likely continue to play a major role in the evolution of novel epithelial appendages.