The localization of tellurium in tellurium-induced hydrocephalus

Résumé L'étude présente concerne la localisation du tellure dans le cytoplasme de cellules nerveuses ftales et post-natales chez des animaux dont la mère a ingéré du tellure durant la grossesse.

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This work was supported by USPHS research grant No. NSO-8891.     

The target period during fetal life for the production of tellurium hydrocephalus

Résumé Le terme de «target period», qui peut se traduire par période-cible, signifie la période de grossesse la plus sensible aux effets nocifs d'un agent tératogène. Le présent travail situe chez le rat la période-cible du tellure entre les 10^e et 15^e jours de la grossesse.

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This work is supported by grant No. NS 8891-01 from the U.S. Public Health Service.     

Period of teratogenic vulnerability of rat embryo to induction of hydrocephalus by tellurium

Zusammenfassung Die intramuskuläre Injektion von metallischem Tellur an trächtige Ratten am 9. oder 10. Tag der Gestation führte bei den Jungtieren zu Hydrocephalie. Bei Verabfolgung des Metalls vor oder nach dieser Periode der Embryonalentwicklung konnte die Missbildung nicht beobachtet werden. Das Auftreten der Hydrocephalie nach Verabfolgung von Tellur korreliert somit mit der «empfindlichen Phase» der ZNS-Entwicklung bei der Ratte.     

Fetal encephalopathy following ingestion of tellurium

Résumé L'ingestion de tellure, associé à la diète normale, par une rate en gestation, peut donner des ratons hydrocéphaliques. Des anomalies cellulaires s'observent dans le cerveau du foetus de 13 à 15 jours.     

The localization of mutarotase in rat kidney

Summary The localization of mutarotase in rat kidney was investigated by fluorescein-labelled and peroxidase-labelled antibody techniques, and by method of isolation of the nuclei and cytoplasm in non-aqueous solvents. In these immunohistochemical studies, mutarotase was almost exclusively recognized in the nuclei of epithelial cells of renal tubules and glomeruli in rat. The specific activity of mutarotase was found to be 1.5 times higher in the nuclei (122 units/g dry wt) than that in the cytoplasm (80 units/g dry wt) isolated with non-aqueous solvents. These results suggest that mutarotase may be involved in the metabolism of D-glucose in nuclei.Acknowledgment. The authors thank Prof. Y. Nishizuka (Department of Biochemistry, School of Medicine, Kobe University) for his valuable advice.     

The localization of mutarotase in rat kidney.

The localization of mutarotase in rat kidney was investigated by fluorescein-labelled and peroxidase-labelled antibody techniques, and by method of isolation of the nuclei and cytoplasm in non-aqueous solvents. In these immunohistochemical studies, mutarotase was almost exclusively recognized in the nuclei of epithelial cells of renal tubules and glomeruli in rat. The specific activity of mutarotase was found to be 1.5 times higher in the nuclei (122 units/g dry wt) than that in the cytoplasm (80 units/g dry wt) isolated with non-aqueous solvents. These results suggest that mutarotase may be involved in the metabolism of D-glucose in nuclei.     

The reticulo-endothelial localization of hepatic acetylcholinesterase

Zusammenfassung Die Acetylcholinesterase wurde histochemisch in den sinusoidal-reticulo-endothelialen Zellen der Kaninchenleber gefunden. Es wird angenommen, dass das Enzym an der Fett- und Esterumwandlung beteiligt ist.     

The localization of kallikrein in the dog and guinea-pig submandibular glands

Summary In the dog and guinea-pig submandibular glands kallikrein seems to be present in the striated duct cells. Following sympathetic nerve and in vivo isoproterenol stimulation of the dog and guinea-pig submandibular gland respectively, there is a reduction of kallikrein concentration. Ultrastructurally this reduction corresponds to the decrease of straited duct secretory granules in both species. Parasympathetic stimulation also causes some release of kallikrein from both species.Supported by Dean's M.R.C. grant to the College of Dentistry.     

The localization of acetylcholinesterase in the optic lobe in the developing chick embryo

Summary The localization of acetylcholinesterase (AChE) in the optic lobe in the developing chick embryo was studied histochemically and biochemically. The histochemical reaction of AChE increased remarkably between stage 42 and 44 especially in the neuropile. The increase of the biochemical activity of the AChE in the synaptic membrane fraction occurred at a later stage than that in the microsomal fraction. These findings can be interpreted as the result of axonal transport of the enzyme from the synthetic to the synaptic site.Acknowledgments. The authors wish to thank Prof. Yasuharu Kuwahara, Cataract Research Institute, for the use of his cryostat and other laboratory facilities, Dr. Koji Kami, Department of Anatomy, Keio University School of Medicine, for his valuable advice regarding histochemistry.     

The localization and functional significance of alkaline phosphatase in the vertebrate ovary

Zusammenfassung Es zeigt sich, dass die Wirkung der alkalischen Phosphatase in der Theca interna, im interstitiellen glandulären Gewebe und im Corpora lutea liegt und eine bedeutende Transportfunktion bei den ovariellen Zellmembranen der Wirbeltiere hat.     

Experimental hydrocephalus following mechanical increment of intraventricular pulse pressure

Summary Experimental hydrocephalus has been induced in lambs by artificial increase of the amplitude of intraventricular cerebrospinal fluid (CSF) oscillations related to arterial pulsations, without concominant changes of the mean CSF-pressure. The characteristics of this hydrocephalus demonstrate that the intraventricular CSF-pulsations can play a role in the genesis of ventricular dilatation. Such a method may be used to produce an original model of hydrocephalus independent of changes of CSF-circulation or absorption.This work was supported by grant of CNR.     

Increased level of 5-hydroxyindoleacetic acid in cerebrospinal fluid from infantile hydrocephalus

Zusammenfassung Die Konzentration der 5-Hydroxyindolessigsäure wurde in der Cerebrospinalflüssigkeit hydrocephalischer Kleinkinder (bis 1 Jahr) bestimmt. Die Werte waren deutlich erhöht. In einigen Fällen wurde die Konzentration an Homovanillinsäure, die ebenfalls erhöht war, bestimmt.     

The localization of3H-corticosterone in mast cell granules by electron microscopic autoradiography

Zusammenfassung Der Einbau von 1,2-³H-Corticosteron in die Mastzellen der lymphatischen Organe wurde bei Mäusen untersucht. Bei gleichzeitiger Anwendung von Autoradiographie und Elektronenmikroskopie konnte das Corticosteron in den Mastzellen nachgewiesen werden.     

Dysplasia of subcommissural organ in congenital hydrocephalus spontaneously occurring in CWS/Idr rats

Summary The subcommissural organ (SCO) of the congenital hydrocephalus spontaneously occurring in CWS/Idr rats was severely reduced in size and displaced at some distance from the anterior end of the cerebral aqueduct. The cerebral aqueduct of the hydrocephalic rats was open throughout its total length during postnatal days 1–20, though it was somewhat narrower at its middle region than in the normal brain.     

Dysplasia of subcommissural organ in congenital hydrocephalus spontaneously occurring in CWS/Idr rats

The subcommissural organ (SCO) of the congenital hydrocephalus spontaneously occurring in CWS/Idr rats was severely reduced in size and displaced at some distance from the anterior end of the cerebral aqueduct. The cerebral aqueduct of the hydrocephalic rats was open throughout its total length during postnatal days 1-20, though it was somewhat narrower at its middle region than in the normal brain.