丽纹龙蜥不同组织的同工酶

采用聚丙烯酰胺凝胶电泳方法，对雄性丽纹龙蜥的脂肪体、精巢、肺、肝脏、肌肉、肠、胃和心脏等8种组织中的酯酶、过氧化物酶、苹果酸脱氢酶、乳酸脱氢酶和醇脱氢酶等5种同工酶进行了研究，结果表明，丽纹龙蜥的5种同工酶存在组织特异性．     

鲇和大口鲇同工酶比较研究

采用聚丙烯酰胺垂直平板凝胶电泳技术，对鲇和大口鲇的肌肉、心脏、肝脏、肾脏、脑、眼等6种组织器官乳酸脱氢酶（LDH）和酯酶（EST）同工酶进行了比较研究。结果表明：2种鲇的同种组织中乳酸脱氢酶（LDH）同工酶的酶谱相同，但在不同组织中表现出明显的组织特异性；而2种鲇的酯酶（EST）同工酶谱表现出明显的组织特异性和物种特异性。笔者还对2种鲇的亲缘关系进行了探讨。     

树(鼠句)(Tupaia belangeri chinensis)消化器官和性腺LDH同工酶的电泳分析

采用0.5%琼脂糖凝胶电泳方法对云南树鼩的肝脏、胃、小肠、胰腺、睾丸和卵巢组织的乳酸脱氢酶同工酶进行电泳分析.结果表明,在6种不同组中,乳酸脱氢酶均呈现为5种不同分子形式的同工酶.其LDH_4至LDH_1同工酶随电泳迁移率的增大依次趋于阳极端,LDH_5同工酶则趋于阴极端.按分光光度比色定量法对各同工酶组分进行洗脱定量,分别测得6种组织中5种同工酶组分的相对百分含量.通过酶谱分析,提示在6种不同组织中由于A、B两种基因表达活性的差异,其酶谱特征也各不相同.文中对树鼩6种组织的LDH同工酶的H和M亚基百分率及H/M亚基比率进行了统计分析,提示这与各类组织的生物学功能及代谢类型相关.     

人工养殖下棘胸蛙的LDH同工酶冬眠前后的变化

利用聚丙烯酰胺垂直板电泳法,研究了平原地区人工养殖的棘胸蛙在冬眠前、中、后三个期间中,肝、肾、心、肌四种组织的乳酸脱氢酶酶谱,发现乳酸脱氢酶同工酶酶谱在不同组织中有差异,且在冬眠前后有一定的变化;初步探讨了季节对LDH同工酶的影响。     

中国鲎同工酶组织器官特异性表达

采用垂直聚丙烯酰胺凝胶电泳技术,分析了中国鲎6种组织器官(肝胰腺、心脏、肠、鳃、肌肉和黄色结缔组织)的乙醇脱氢酶(ADH)、山梨醇脱氢酶(SDH)、酯酶(EST)、过氧化氢酶(CAT)、天冬氨酸转化酶(AAT)、苹果酸脱氢酶(MDH)和苹果酸酶(ME)等7种同工酶的活性和分布,并对其酶谱的表型进行了分析.结果表明,中国鲎的同工酶系统存在不同程度的组织特异性.乙醇脱氢酶在鳃和黄色结缔组织中不表达;山梨醇脱氢酶在鳃中不表达;酯酶、过氧化氢酶和苹果酸脱氢酶在6种组织器官中均有表达,但表达活性存在差异;而天冬氨酸转化酶Aat-1位点在鳃中不表达.     

大银鱼Protosalanx hyalocranius(Abbott)不同组织LDH同工酶、EST同工酶的初步研究

报道了应用聚丙烯酰胺凝胶垂直板电泳(PAGE)研究大银鱼Protosalanxhyalocra nius(Abbott)胚胎发育不同时期及成体肝脏、骨骼肌、肾脏、眼等几种组织器官中LDH同工酶(乳酸脱氢酶同工酶)电泳图谱、EST同工酶(脂酶同工酶)电泳图谱.结果表明:大银鱼LDH有三条酶带;EST有两条酶带.分析表明LDH同工酶、EST同工酶在活力和组织分布方面均具有特异性.     

黑冀地鳖和中华真地鳖三种同工酶的比较研究

用聚丙烯酰胺垂直平板凝胶电泳法分析比较了新疆黑冀地鳖polyphaga obscurachop和中华真地鳖Eupolyphaga sinensis Walker的酯酶(EST)、苹果酸脱氢酶(MDH)和乳酸脱氢酶(LDH)三种同工酶的电泳酶谱,研究结果表明:两种地鳖各自具有不同的特征性酶谱;不同组织具有不同的特征性酶谱;酯酶(EST)同工酶的两种地鳖中的活性均较高,而乳酸脱氢酶(LDH)同工酶的活性则较低.     

湖北侧褶蛙与黑斑侧褶蛙同工酶的比较研究

采用垂直板聚丙烯、酰胺凝胶电泳方法，对湖北侧褶蛙(Pelophylax hubeiensisi)和黑斑侧褶蛙(Pelophylax mgromaculata)的消化道、心脏、肌肉、脾脏、肾脏、脑、肝脏、生殖腺、晶体和肺等10种组织中的酯酶(EST)、苹果酸脱氢酶(MDH)、乙醇脱氢酶(ADH)、乳酸脱氢酶(LDH)、过氧化物酶(POX)等5种同工酶进行了比较研究。分析并讨论了5种同工酶在不同组织中的分布及遗传基础。结果显示，两种蛙的POX、MDH、ADH同工酶表现明显的遗传相似性；EST、LDH同工酶存在较大的种间差异。     

密点麻蜥冬眠期和繁殖期几种器官组织中乳酸脱氢酶(LDH)同工酶的比较研究

采用聚丙烯酰胺凝胶电泳法,对冬眠期和繁殖期密点麻蜥(Tremies multidcelate)6种器官组织中乳酸脱氢酶(LDH)同工酶酶带和酶活性进行了测定和分析研究.结果表明:不同组织的酶带明显不同,且有多画带现象;6种器官组织中LDH同工酶的相对活性表现出明显的季节差异.     

黄河鲤鱼乳酸脱氢酶同工酶研究

本文采用聚丙烯酰胺凝胶垂直平板电泳，分析了黄河鲤鱼Ｃｙｐｒｉｎｕｓ ｃａｒｐｉｏｈａｅｍａｔｏｐｔｅｒｕｓ的脑、肝、心、肾、肌、眼六种器官的乳酸脱氢酶（ＬＤＨ）同工酶。结果表明：在不同器官中乳酸脱氢酶同工酶存在着明显的组织特异性；比较黄河鲤鱼与鲤鱼Ｃｙｐｒｉｎｕｓ Ｃａｒｐｉｏ Ｌ．及其它鲤科鱼类乳酸脱氢酶同工酶酶谱，除它们的酶谱相似外，黄河鲤鱼乳酸脱氢酸同工酶表现有一定的特异性，这种特异性可能是     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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Brief Introduction to “Journal of Jilin University( Science Edition) ”

<正>"Journal of Jilin University(Science Edition)"is a comprehensive academic journal in the fields of science sponsored by Jilin University and administrated by the Ministry of Education of the Peoples Republic of China.The journal started publication in 1955.The original name at starting publication was"Journal of Natural Science of Northeast People University",which was changed into"Acta Scientiarum Naturalium Universitatis Jilinensis"in 1958 owing to the name change of the university.The present journal name has