高锰酸钾用于铜离子的可视化检测

建立了以高锰酸钾作指示剂的可视化检测铜离子的分析方法,并探讨了反应机理.讨论了利用本体系测定铜离子的最佳条件,结果表明,在p H为9.62的BR缓冲溶液中,检测Cu2+的线性范围为5.0×10-8~1.0×10-6mol/L,相关系数为r=0.998 4.该方法检出限低,线性范围宽,方法简便,应用于环境水样中Cu2+检测,获得了较好的结果.     

The condensing vacuole of exocrine cells is more acidic than the mature secretory vesicle

A number of intracellular, membrane-bound compartments in both the endocytic and exocytic pathways of eukaryotic cells have an acidic internal pH. In endocrine cells, the mature secretory vesicle has an acidic pH; secretory vesicles isolated from exocrine cells, however, appear to have a neutral pH. Recently we have used a newly developed immunocytochemical technique to map low-pH compartments in insulin-secreting islet cells with the electron microscope and find that during the maturation of the secretory vesicle there is a progressive acidification of these vesicles that begins as soon as the trans Golgi condensing vacuoles form. Now we have used this technique to examine two exocrine cells: the pancreatic acinar cell and the parotid serous cell. In both cell types, the trans Golgi condensing vacuoles are acidic and accumulate the low-pH probe to the same extent as condensing vacuoles of insulin-secreting islet cells. Unlike insulin-secreting cells, however, maturation of the granules is accompanied by a return of luminal pH to near neutrality. Therefore, although the pH of storage granules in exocrine and endocrine cells is different, the pH of the condensing vacuoles in both cells is acidic.     

Regulated import and degradation of a cytosolic protein in the yeast vacuole.

The key regulatory enzyme in gluconeogenesis, fructose 1,6-bisphosphatase (FBPase) is subject to glucose-stimulated proteolytic degradation in Saccharomyces cerevisiae. This process involves the regulated transfer of FBPase directly from the cytosol into the vacuole or a vacuole-related organelle. Glucose may regulate the production of an FBPase receptor or import factor that is transported to the vacuole through the secretory pathway.     

植物细胞质化石研究进展

 近年来古植物学研究表明,植物细胞质确实可以保存为化石。随着相关研究技术的进步,研究发现植物化石中具有和现代植物相似的超微结构。特殊情况下,植物细胞质化石研究能够帮助解决现代生物学中某些难以解决的问题。高温和野火在植物细胞质化石的形成过程中起到了重要作用,而一个过去被古生物学家视而不见的自然现象--雷电可能对某些超微结构的固定起到了关键作用。植物细胞质化石研究把古植物学推向一个新的研究层面和方向,促进了与其他学科之间的融合,也把一些新的研究手段引入到了古生物学中。本文回顾植物细胞质化石的研究历程,总结研究成果和经验,并对未来发展趋势进行了展望。