大肠杆菌新菌毛抗原的研究V．用标记抗体技术检验菌毛抗原

应用荧光抗体染色、免疫酶染色及斑点酶联免疫吸附试验（Ｄｏｔ－ＥＬＩＳＡ）对大肠杆菌Ｆ１９８７新菌毛抗原进行了检验，经可行性、特异性及对粪便标本检验等试验，表明这些方法均可用于对Ｆ１９８７新菌毛抗原的检出。三种方法除均表现特异、准确外，又分别具有各自的特点。荧光抗体染色结果易分辨，但需具备荧光显微镜；免疫酶染色只需普通显微镜；Ｄｏｔ－ＥＬＩＳＡ可用眼观判定结果，但对病料标本需做一定的处理后才能进行检验。本次试验均采用了间接染色法，同时试验建立了三种方法对Ｆ１９８７新菌毛抗原的具体操作程序。     

应用miniVIDAS检测食品中沙门氏菌效果分析

采用miniVIDAS和GB法同时对不同种类食品进行沙门氏菌检测,评价自动酶联荧光免疫分析系统检测食品的灵敏度和特异性。     

抗紫杉醇抗体的制备方法

用化学方法将紫杉醇（Ｔａｘｏｌ）与牛血清白蛋白（ＢＳＡ）偶联制成全抗原Ｔａｘｏｌ－ＢＳＡ。以Ｔａｘｏｌ－ＢＳＡ免疫家兔后收集抗血清，分离纯化抗体，经酶联免疫吸附法测定证实抗血清中有特异性抗紫杉醇抗体，将纯化的抗体进行免疫双扩散鉴定，结果表明其有较高滴度（３．２×１０＾２）。     

抗紫杉醇抗体的制备方法

用化学方法将紫杉醇（Ｔａｘｏｌ）与牛血清白蛋白（ＢＳＡ）偶联制成全抗原Ｔａｘｏｌ－ＢＳＡ．以Ｔａｘｏｌ－ＢＳＡ免疫家兔后收集抗血清，分离纯化抗体，经酶联免疫吸附法测定证实抗血清中有特异性抗紫杉醇抗体，将纯化的抗体进行免疫双扩散鉴定，结果表明其有较高滴度（３．２×１０２）．     

纯化抗原半定量检测幽门螺杆菌感染

目的：鉴定并提取幽站螺杆菌（Ｈｐ）特异性抗Ｈｐ抗体的半定量免疫酶技术。方法：１０株Ｈｐ可溶性蛋白用于十二烷基磺酸钠－聚丙烯酰胺凝胶电泳和免疫印迹分析，凝胶怪析提取特异性抗原，方阵滴定建立免疫酶方法。纯化怕与全菌抗原同批检测１３６例患，以免疫印迹分析作为金标准。结果：Ｈｐ主要特异性怕分子量为６６０００，６９０００，３１０００和２５０００，ＳｅｐｈａｄｅｘＧ－２００柱层析第１提取物含其中三种怕成分。     

两种不同示踪剂用于蛋白质与抗原分子微阵列信号检测的对比研究

以氧化型琼脂糖凝胶膜为基片，制作蛋白质与抗原分子微阵列，建立了检测固相人IgG,游离IgG和自身抗体分子的免疫学测定模式，分别以辣根过氧化物酶－二氨基联苯胺一双氧水（HRPDAB－H2O2）和胶体金－硝酸银－对苯二酚2种示踪系统作为蛋白质与抗原分子微阵列信号显示剂，并对其作用的原理与特点进行讨论，检测结果发现基于胶体金的免疫金银染色法（IGSS）比固相酶免疫测定法（EIA）敏感，2种示踪系统对固相人IgG的最低检出量分别为0．05ng和0．5ng；而对血清游离IgG的最低检出量IGSS法比EIA法至少高出10倍，两法用于自身抗体的测定亦表现出较好的重复性，在14例HCV感染者中，自身抗体的总体检出率分别为38．6％和31．4％，结果具有显著的同步性，故同时辅以2种示踪剂并用于阵列芯片的联合测试，有助于提高自身抗体的总体检出水平。     

新型肾综合征出血热EIA诊断试剂盒的研制

用多肽合仪合成汉坦病毒核衣壳蛋白（NP）(aa17－66）50个氨基酸残基的核心序列,以此为抗原,建立特羿性检测抗汉坦病毒IgG和IgM的酶联免疫吸附剂测定法（ELISA）,通过实验发现这一序列具有较强的抗原性,研制出了以合成肽为抗原的新型牧场异性检测抗汉坦病毒IgG和IgM的诊断试剂盒,通过对肾综合征出血热抗体阳性血清的检测,效果良好。     

免疫转印技术对人结肠Mr40KD蛋白粗提物中干扰成份的分析

目的：分析ELISA包被抗原在检测溃疡性结肠炎（UC）血清ACA的非特异性干扰物。方法：应用免疫转印技术，结合酶标抗人IgG抗体和荧光抗人IgG抗体对含Mr40KD蛋白包被抗原的人结肠提取物进行纯度和免疫学性质的分析。结果：包被抗原粗提物中除含有Mr40KD蛋白外，还含有过氧化物酶和人IgG活性成份。给论：过氧化物酶和人IgG活性成份是应用ELISA法检测UC血清ACA－IgG的非特异性干扰物，需进一步纯化处理。     

高香草酸—H2O2—HRP荧光酶联免疫分析测定HRP的研究

提出了高香草酸－Ｈ２Ｏ２－ＨＲＰ荧光酶联免疫分析测定ＨＲＰ的新方法。该法基于ＨＲＰ催化Ｈ２Ｏ２氧高香草酸生成能产生荧光的物质，该物质在Ｅ＝３１７ｎｍ激光作用下产生荧光，     

ELISA技术及在甘肃马铃薯脱毒种薯生产中的应用现状

酶联免疫吸附（Ｅｎｚｙｍｅ－ＬｉｎｋｅｄＩｍｍｕｎｏｓｏｒｂｅｎｔＡｓｓａｙ）测定即ＥＬＩＳＡ技术，是近年来继免疫荧光技术与放射免疫分析法之后发展应用于植物病毒检测的新方法。它不仅可以定量测定抗体，也可以鉴定抗原，具有灵敏度高、特异性强、测定方法简单、便于直接观察、可快速大规模检测样品的特点。目前已广泛应用于生物学和医学的许多领域，成为检测鉴定病毒的最新血清学方法之一。１ＥＬＩＳＡ技术的起源与发展１９９６年Ｎａｋａｎｅ和Ａｖｒａｍｅａｓ分别用酶代替荧光色素标记抗体，进行生物组织中抗原的鉴定及定位…     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with