Lymphatic metastasis of tumour; persistent transport of cells.

A model of lymphatic metastasis established by injecting Walker rat carcinoma cells into the rat footpad was used to study the output of tumour cells from the footpad. The lymphatic efferent from the footpad was cannulated in a group of rats with advanced neoplasm; it was shown that the output of tumour cells was continuous over periods up to 90 min and ranged from 10(2)-10(5) cells/min.     

Lymphatic metastasis of transplantable animal neoplasms

Résumé L'injection de 5 millions de cellules tumorales RD3 dans la patte du rat, fait apparaître des dépôts métastasique sous forme de ganglions lymphatiques poplitétaux. L'examen histologique détaillé montre qu'ils sont vraies métastases. Des résultats pareils sont apparus dans 2 autres tumeurs qui étaient histologiquement malignes, mais non dans celles qui étaient bénignes. Cette technique semble convenir à étude de la métastase lymphatique.     

Increased release of tumour cells by collagenase at acid pH: A possible mechanism for metastasis

Summary The ability of collagenase to disaggregate a solid metastasizing lymphosarcoma has been shown to considerably increase with reducing environmental pH. It is suggested that this effect may be operating in vivo to release cells from a primary tumour.     

Increased release of tumour cells by collagenase at acid pH: a possible mechanism for metastasis.

The ability of collagenase to disaggregate a solid metastasizing lymphosarcoma has been shown to considerably increase with reducing environmental pH. It is suggested that this effect may be operating in vivo to release cells from a primary tumour.     

On the prevention of haematogenous tumour metastasis to liver and lung

Summary Vascular endothelium is lined by a layer of alpha, 2, macroglobulin (AMG). This protide is believed to regulate the rate of extravasation of tumor cells. Experiments are described which show that increasing the deposition of AMG (enhancing its production and blocking its lysis) reduces the incidence of tumour metastases to liver and lung.     

Pea phytohemagglutinin selective agglutination of tumour cells

Zusammenfassung Phytohaemagglutinin, aus der ErbsePisum sativum L. isoliert, agglutiniert vornehmlich Tumorzellen, ähnlich wie Concanavalin A. Die durch RNS-onkogene Viren transformierten sowie spontan transformierten Zellen befolgen während der Agglutination das normale Muster der Tumorzellen-Interaktion mit Concanavalin A oder Weizenkeim-Agglutinin.     

Effect of biguanides on the respiration of tumour cells

Riassunto È stato studiato l'effetto di un gruppo di derivati biguanidici sulla respirazione in vitro di cellule tumorali ed epatiche. I composti esaminati sono tutti capaci di inibire, in vario grado, lo sviluppo del tumore ascite di Ehrlich del topo, tranne uno, privo di attività antitumorale in vivo. Tutti i composti mostrano di deprimere lievemente il consumo di ossigeno delle cellule epatiche. I derivati attivi in vivo bloccano quasi completamente la respirazione delle cellule tumorali, mentre il composto inattivo ha un'attività assai inferiore. Si fa l'ipotesi che l'azione antitumorale dei derivati biguanidici si esplichi attraverso una inibizione dei processi respiratori collegati alla formazione di legami altoenergetici.     

Experiments on the tumour cells using time-lapse microcinematography

Riassunto Viene descritta mediante riprese microcinematografiche a tempo in cellule del tumore di Walker trattate con desossiribonucleasi la formazione graduale di globuli conseguenti alla aggregazione di ribosomi. Si Riritiene pertanto che venga confermata l'ipotesi che nella cellular tumorale esista una condizione particolare per cui, ad una anormale produzione di ribonucleoproteine, non segua la produzione di concreti prodotti di sintesi.     

Lymphatic drainage of the brain

Zusammenfassung In krassem Gegensatz zur heute herrschenden Auffassung spielt eine Lymphdrainage beim Flüssigkeitskreislauf des Gehirns eine ausschlaggebende Rolle. Werden die zervikalen Lymphbahnen verschlossen, so entsteht eine lymphostatische Enzephalopathie, welche u.a. durch ein Hirnödem, Ganglienzell- und Myelinveränderungen sowie durch Beeinträchtigung der Funktion des Zentralnervensystems gekennzeichnet ist. Es wird ein Durchbruch der Lymphologie in das Gebiet der Neurologie und Neuropathologie gefordert und darauf hingewiesen, dass bei der Pathologie des Hirnödems die Rolle der Lymphzirkulation nicht ausser acht gelassen werden sollte.     

Lipid transport pathways in mammalian cells

Summary A major deficit in our understanding of membrane biogenesis in eukaryotes is the definition of mechanisms by which the lipid constituents of cell membranes are transported from their sites of intracellular synthesis to the multiplicity of membranes that constitute a typical cell. A variety of approaches have been used to examine the transport of lipids to different organelles. In many cases the development of new methods has been necessary to study the problem. These methods include cytological examination of cells labeled with fluorescent lipid analogs, improved methods of subcellular fractionation, in situ enzymology that demonstrates lipid translocation by changes in lipid structure, and cell-free reconstitution with isolated organelles. Several general patterns of lipid transport have emerged but there does not appear to be a unifying mechanism by which lipids move among different organelles. Significant evidence now exists for vesicular and metabolic energy-dependent mechanisms as well as mechanisms that are clearly independent of cellular ATP content.     

Nuclear projections in tumour cells and large chromosome markers

Summary The application of banding techniques on cytological smears from pleural effusion in a case of histiocytic sarcoma has provided direct evidence for correspondence between nuclear projections in tumor cells and extra large chromosome markers observed in the neoplastic karyotype obtained by direct preparations.