河北雾灵山地衣的研究Ⅲ(石耳科Umbilicariaceae、黄枝衣科Teloshistaceae、蜈蚣衣科Physciaceae)

本研究对采自河北省雾灵山的石耳科Umbilicariaceae、黄枝衣科Teloshistaceae、蜈蚣衣科Physciaceae地衣标本的形态、结构及化学成分进行了分析鉴定,研究结果表明:雾灵山的石耳科有石耳属(Umbilicaria)1种:绒毛石耳Umbilicaria vellea;黄枝衣科有石黄衣属(Xanthoria)2种:中国石黄衣Xanthoria mandschurica和阿佛列石黄衣Xanthoria alfredi;蜈蚣衣科黑蜈蚣衣属(Phaeophyscia)4种:毛边黑蜈蚣衣Phaeophyscia hispidula;白刺毛黑蜈蚣衣Phaeophyscia hirtuosa;裂芽黑蜈蚣衣Phaeophyscia exornatula;刺黑蜈蚣衣Phaeophyscia primaria;雪花衣属(Anaptychia)1种:毛边雪花衣Anaptychia ciliaris(L.)K b..其中,裂芽黑蜈蚣衣Phaeophyscia exornatula属于河北省地衣的新记录种.本工作为雾灵山地衣的区系研究奠定了基础.     

高层住宅外墙面污染(渗漏)预防及治理

建筑物外墙被雨(雪)水污染的原因是当雨(雪)水沿垂直面下流侵入墙的毛细管中并沿横向毛细管侵入任何深度,而表面粗糙的墙面阻碍了雨(雪)水在墙面上的流速,并在风压的作用下,增加了水渗入横向毛细管的数量和速度.为了阻止雨(雪)水毛细作用,防止雨(雪)水将污染物带到墙面,并渗透墙体,唯一有效的方法就是做好滴水槽(滴水线)和流水坡度,加强细部做法的施工质量.     

基于小波融合的视频图像去雨(雪)方法

户外视觉系统在雨(雪)天气条件下获取的视频图像会产生严重退化.提出一种基于小波融合的视频图像去雨(雪)方法,从频率域角度分析,采用小波多层分解和小波融合技术:先确定雨(雪)噪声所在的具体层,再制定一种基于雨(雪)噪声污染度的融合规则,最后对多幅连续退化图像的特定层进行小波融合,以达到去除雨(雪)的目的.仿真结果表明,本文方法的复原结果理想,不受噪声强度制约.     

福建省新分布植物(Ⅱ)

报道了福建被子植物2个新记录属:肋柱花属(Lomatogonium)、庭菖蒲属(Sisyrinchium),6个新记录种:大花肋柱花(Lomatogonium macranthum)、黄花庭菖蒲(Sisyrinchium iridifolium)、水香薷(Elsholtzia kachinensis)、小花月见草(Oenothera parviflora)、裂叶月见草(Oenothera laciniata)和纤草(Burmannia itoana),其中黄花庭菖蒲、小花月见草和裂叶月见草为本省新归化植物,并提供了相应的生境或特写图片.凭证标本均保存于福建师范大学生命科学学院植物标本馆(FNU).     

路面主动融冰(雪)技术的发展与展望

针对目前路面融冰(雪)技术存在滞后性、效果差、能耗大等问题,分别从刚性路面、柔性路面两个角度,深入研究了6种路面主动融冰(雪)技术的现状、应用效果及发展前景.对其优、缺点以及适用地区进行了全面的分析,为解决冬季道路冰雪问题提供了系统的思路和途径.     

河北省地衣新记录(Ⅰ)

在研究河北省地衣资源的过程中,首次报道了河北省产11种地衣新记录种:苔生双缘衣(Diploschistes muscorum)、裂杯石蕊(Cladonia rei)、蒙古石蕊(Cl.mongolica)、矮石蕊(Cl.humilis)、多毛猫耳衣(Leptogium hirsutum)、土星猫耳衣(L.saturninum)、短根条衣(Everniastum nepalense)、圆叶黑蜈蚣衣(Phaeophyscia orbicularis)、斑面蜈蚣衣(Physcia aipolia)、兰灰蜈蚣衣(P.caesia)和石黄衣(Xanthoria parietina).其中,双缘衣属(Diploschistes)为河北省新记录属.对11种地衣的基物、识别特征和地理分布特点作了初步的讨论.     

河北省地衣新记录(Ⅱ)

报道了采自河北省7种地衣新记录种:裸果猫耳衣Leptogium hildenbrandii、变兰猫耳衣L.cyanescens、薄刃猫耳衣多叶变种L.moluccanum var.myriophyllinum、颈外蜈蚣衣Hyperphyscia syncolla、拟菊叶黄梅Xanthoparmelia taractica、粉大孢蜈蚣衣Physconia distorta、污白雪花衣Anaptychia ulotricoides.对7种地衣的生境、识别特征和地理分布特点进行了简要概述.     

厚唇光唇鱼(Acrossocheilus labiatus)和侧条光唇鱼(A.parallens)的比较研究

通过对其形态、解剖及部分同工酶表型的比较研究,发现厚唇光唇鱼(Acrossocheiluslabiatus)和侧条光唇鱼(A.parallens)的差别比较显著,认为二者都是有效种,但它们各自占领不同的水域,其分布受到南岭的阻隔.     

实验动物科学(Laboratory Animal Science)1985年第35卷第三期

生物医学研究中的雪貂 1.雪貂、狗、猫的视网膜毯、视网膜、颅骨的比较研究。A comparative Study orthe TaPetum,Retina and Skull of theFerret,Dog and Cat.//G.Ywen,JA Sturman,J W Shek······……200 2.雪貂在视觉系统研究币的应用。useof Ferret in Studies of the     

诸葛菜属及近缘类群(十字花科)的幼苗形态及其分类学意义

对中国特有属诸葛菜属 Orychophragmus 4种植物及近缘的心叶碎米荠 Cardamine limp richti‐ana 、大叶葱芥 A llaria grandi f olia 等的幼苗进行了形态学观察,并探讨了分类学意义.结果表明:1)诸葛菜属4种植物与心叶碎米荠、大叶葱芥幼苗的子叶均对折,出土,其脉序为掌状网脉,具侧生一级至四级脉,诸葛菜 O . violaceus 、大叶葱芥和心叶碎米荠3种具少数五级脉;2)在诸葛菜属内,诸葛菜的幼苗初生叶的毛被和子叶脉序与其他3种(湖北诸葛菜 O .hupehensis 、太白诸葛菜 O .taibaiensis 、铺散诸葛菜 O .di f f usus)存在明显差异,其中铺散诸葛菜第二、第三真叶羽裂.诸葛菜的下胚轴长度、直径明显长于或粗于其他3种 ;3)心叶碎米荠、大叶葱芥的幼苗与诸葛菜相似,但初生叶边缘具圆齿而有别,与铺散诸葛菜等3种在子叶脉序上差异较大,子叶与异堇叶碎米荠 C .violi f olia var .diversi f olia也有很大差别;4)全面观察幼苗形态并结合形态特征、花粉微形态、体细胞染色体数和系统发育研究结果,不支持将铺散诸葛菜、湖北诸葛菜和太白诸葛菜并入诸葛菜,也不支持将心叶碎米荠和大叶葱芥归入诸葛菜属.     

Agrobacterium tumefaciens-mediated Transformation of CryⅠA(b) gene to Trichoderma harzianum

In this study, Cry ⅠA(b) gene was successfully transferred into the biocontrol fungus Trichoderma harzianum with an efficiency of 60-180 transformants per 10^6 spores by using Agrobacterium tumefaciens-mediated transformation. Putative transformants were analyzed to test the presence of Cry ⅠA(b) gene by Southern blot. Most transformants contained a single T-DNA copy. RT-PCR analysis showed that the Cry ⅠA(b) gene was transcribed. Antifungal activities and insecticidal activities of the transformants were examined. There was no obvious difference in antifungal activities between the transformants and their wild strains. The modified mortalities of the transformants T1 and T2 were 69.57% and 91.30%, respectively. The tranformation system mediated by A. tumefaciens proved to be a powerful tool for the filamentous fungi transformation and functional genomic study with its high transformation frequency, simplicity of T-DNA integration, and genetic stability of transformants.     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

入侵植物紫茎泽兰叶斑真菌多样性及其致病性研究

 从云南、广西等12个地区的紫茎泽兰和其他8种植物叶片上共分离得到47个真菌菌株.根据菌落、菌丝以及孢子形态,结合18SrRNA基因限制性酶切片段长度多态性(RFLP)分型和序列测定分析,47个菌株共分为了19个类群,系统发育关系与Aspergillusniger,Cladosporiumcladosporioides,Sporisoriumreilianum,Colleto-trichumlupini,Alternaria sp.,Sporobolomycesroseus,Dothideomycetesp.,Phoma sp.,Filobasidiumelegans,Aureobasid-iumpullulans,Rhodotorulaslooffiae,R.glutinis,Penicilliumdecumbens,Pestalotiopsismaculans,Cryptococcusaureus,Bimurianovae-zelandiae,Xylariaceae sp.类似.这些菌株中仅有5个对紫茎泽兰离体叶片表现致病性,在系统发育树上分别和Myrothecium sp.,C.lupini,Alternaria sp.,C.cladosporioides,Dothideomycete sp.聚类.有2个R.gluti-nis菌株对马铃薯和白菜有致病作用,但对紫茎泽兰没有致病力.     

谷氨酰胺转胺酶发酵培养基的响应面分析优化

利用SAS（Statistical Analysis System）软件中二水平设计的Plackett-Burman设计和响应面分析法（Response Surface Analysis，简称RSA），研究了链霉菌（Streptomyces sp.）HS-1摇瓶发酵生产谷氨酰胺转胺酶的发酵培养基.通过二水平设计实验考察了八种因素对发酵生产谷氨酰胺转胺酶的影响，利用极差分析确定其中以豆饼粉水解物、甘油以及硫酸铵为影响链霉菌发酵生产谷氨酰胺转胺酶的重要因素.通过响应面分析法建立了这三个重要因素的二次回归模型，应用规范分析找到最优点，分别为豆饼粉水解物24.0 g &;#8226; L^-1，甘油24.4 g &;#8226; L^-1，硫酸铵5.40g &;#8226; L^-1.摇瓶实验表明，应用优化培养基谷氨酰胺转胺酶酶活由6.27 μ &;#8226; mL^-1提高到7.30μ &;#8226;mL^-1，提高了16.4%.     

Induction of nicotine in tobacco by herbivory and its relation to glucose oxidase activity in the labial gland of three noctuid caterpillars

Tobacco Nicotiana tabacum L. is a host plant ofHelicoverpa armigera (Huibner), Helicoverpa assulta Guenee and Spodoptera litura (Fabricius) (Lepidoptera, Noctuidae). The difference in leaf nicotine response to the feeding by these three larvae and the mechanical simulation of their feeding was examined by HPLC. Results indicated that nicotine induction was suppressed by H. armigera and H. assulta larvae feeding or by simulated damage treated with their labial glands extracts. The production of nicotine was also suppressed by the glucose oxidase from Aspergillus niger when it was treated on mechanically wounded leaf area. On the contrary, the nicotine production was stimulated by S. litura larva feeding or by simulated damage treated with its labial gland extract. Heat denature can not counteract the stimulation effect of the S. litura labial gland extracts to tobacco nicotine production. The glucose oxidase activity was detected in labial gland extracts of both H. armigera and H. assulta, but the activity in H. armigera was significantly higher than that in H. assulta. No glucose oxidase activity was detected in labial gland extracts of S. litura. It is shown that the glucose oxidase activity in labial glands of caterpillars plays an important role in the nicotine response to herbivory. The glucose oxidase was mainly contained in the labial gland of H. armigera larva, and had the highest activity at pH 7.0. D-Glucose was the optimal substrate of the glucose oxidase. Labial gland glucose oxidase activities varied daily during larval development with high activities found when larvae were actively feeding.     

WIND observations of plasma waves inside the magnetic cloud boundary layers

Based on the WIND observational data for the plasma waves from thermal noise receptor （TNR） working on the frequency 4-256 kHz and the solar wind and the magnetic fields, we analyze the plasma wave activities in the 60 magnetic cloud＇s boundary layers （BLs） and find that there are often various plasma wave activities in the BLs, which are different from those in the adjacent solar wind （SW） and the magnetic clouds （MC）. The basic characteristics are that： （1） the enhancement of the Langmuir wave near the electronic plasma frequency （fpe） is a dominant wave activity, which occupies 75% investigated samples; （2） the events enhanced both in the langmuir and ion acustic （f〈fpe） waves are about 60% of investigated samples; （3） broadband, continuous enhancement events in the plasma wave activities were observed in the whole frequency band of TNR, and about 30% of the 60 samples, however, were not observed in the SW and the MC investigated events; （4） although the ratio of the temperatures between the electon and proton, Te/Tp ≤1, the ion caustic wave enhancement activities are still often observed in the BLs, which makes it difficult to explain them by the traditional plasma theory. New results reported in this paper further show that the magnetic cloud＇s BL is an important dynamic structure, which could provide useful diagnosis for understanding the cloud＇s BL physics and could expand a space developing space plasma wave theory.     

双团棘胸蛙(Paa yunnanensis)肌肉营养成分分析与评价

 对双团棘胸蛙的主要食用部分(肌肉)中的水分、蛋白质、脂肪和灰分质量分数及氨基酸的组成进行了测定,结果显示:水分占鲜重的78.01%,蛋白质占19.77%,脂肪占1.13%,灰分为1.09%,与其他蛙类相比没有显著差异.肌肉中氨基酸占干物质总量的70.14%,其中必需氨基酸质量分数较高,占总氨基酸的46.98%,高于棘胸蛙、棘腹蛙、牛蛙和虎纹蛙;鲜味氨基酸占氨基酸总量的37.34%,接近棘胸蛙和虎纹蛙,而远高于棘腹蛙和牛蛙.还将双团棘胸蛙营养价值与其瘦猪肉、牛肉、牛乳和鸡蛋进行比较与分析,从营养学的角度评价了双团棘胸蛙肌肉营养价值高而且味道鲜美,为今后该物种的保护与合理利用提供基础数据.     

Crystal structure, magnetic and photoelectric properties of coordination polymer[ Co2（C3H4N2）4（ C10H2O8） ]n

The coordination polymer is designed and synthe-sized through the choice of organic ligand and coordina-tion geometry of metal ion as well as the control over theinfinite network topology structure, which usually con-tains both the characteristic of the organic molecules andmetal ions. So, it exhibits more singular function and haspotential application in optics, electrics, information, ca-talysis, medicament, metallurgy, novel material and lifesciences fields. And it has been the confluence m…     

利用Uq(sp(8))的向量表示实现辛型量子群(O)(Spq(8))

Jantzen[1]利用A型李代数A2的量子包络代数Uq(sl2(C)),借助于其表示论并利用R-矩阵的方法给出了SLq(2)的定义关系式.本文对结构更为复杂的C型李代数做了类似的研究,通过Uq(sp(8))的表示理论实现了(O)(Spq(8))的定义关系式.     

α-硫辛酸和二氢硫辛酸的抗氧化作用

采用多种化学发光体系和比色体系研究了氧化型硫辛酸——α-硫辛酸（α-lipoic acid, LA）和还原型硫辛酸——二氢硫辛酸（dihydrolipoic acid, DHLA）直接清除活性氧、抗脂质过氧化以及对&;#8226;OH引起的DNA氧化损伤的保护作用.实验发现，LA和DHLA能有效清除相应体系中的活性氧过氧化氢（H₂O₂）、羟自由基（&;#8226;OH）、过氧亚硝基阴离子（ONOO^-）和二苯代苦味肼基自由基（DPPH&;#8226; ），能显著抑制脂质过氧化，并对&;#8226;OH引起的DNA氧化损伤有良好的保护作用，而DHLA还可以有效清除超氧阴离子自由基（O&;#8226;₂^-）.这些结果提示，LA和DHLA都是良好的抗氧化剂，但相比之下DHLA的抗氧化能力要显著强于LA.本研究为LA和DHLA保健和药用价值的进一步开发，提供了自由基生物学方面的依据.