Regional distribution of thiamin pyrophosphokinase in rat brain

Summary The highest specific activity of thiamin pyrophosphokinase was found in the cerebellum, and lower activity in cerebral cortex and midbrain. The regional difference in the enzyme activity was similar to that in thiamin content and the influx rate in rat brain, suggesting that the enzyme is involved in the thiamin transport.     

Regional distribution of lactate dehydrogenase isoenzymes in adult rat brain.

The highest lactate dehydrogenase (LDH) activity was found in thalamus, statistically significantly less in cerebral and cerebellar cortex and the lowest in pons. LDH1 and LDH4+5 represented 58% and 23% of the total activity in cerebral cortex, 54% and 20% in thalamus, 42% and 4% in cerebellar cortex and 55% and 7% in pons, respectively.     

Regional distribution of adenine nucleotides,glycogen, glucose and lactate in the adult rat brain

Résumé Le cervelet contient des quantités plus grandes d'ATP, d'ADP et de nucléotides totaux que le lobe frontal du cerveau ou la moelle cervicale. Ces différences ne sont pas trouvées avec l'AMP. Le contenu en glycogène, en glucose et en lactate est plus grand dans la moelle cervicale que dans le cervelet et dans le lobe frontal.

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We wish to thank Dr.H. Rehkämper from Boehringer Mannheim GmbH, who kindly supplied us with all the enzymes, substrates and cofactors.     

Regional and subcellular distribution of superoxide dismutase in brain

Summary Superoxide dismutase has been found to be widely distributed and of approximately the same specific activity in all regions of human brain examined. It is not reduced during degeneration of the basal ganglia in Huntington's Chorea. After subcellular fractionation of human and guinea-pig cerebral cortex, the highest specific activity of the enzyme was found in the soluble fraction.Acknowledgments. We thank the Huntington's Chorea Foundation for financial support. Address reprint requests to the Neurochemistry Lab., V. A. Hospital, Seattle, Wash. 98108, USA.     

Topographical distribution of ATP in rat brain

Summary Studies on the distribution of ATP in microdissected segments of the rat brain indicate that the nucleotide is concentrated in gray matter, and especially in the thalamus, hypothalamus, hippocampus, entorhinal cortex and sensorimotor cortex. These distribution studies in conjuction with previous neuropharmacological studies, support the concept that adenine nucleotides may function as intercellular mediators in various regions of the brain.     

Regional and subcellular distribution of superoxide dismutase in brain.

Superoxide dismutase has been found to be widely distributed and of approximately the same specific activity in all regions of human brain examined. It is not reduced during degeneration of the basal ganglia in Huntington's Chorea. After subcellular fractionation of human and guinea-pig cerebral cortex, the highest specific activity of the enzyme was found in the soluble fraction.     

Topographical distribution of ATP in rat brain.

Studies on the distribution of ATP in microdissected segments of the rat brain indicate that the nucleotide is concentrated in gray matter, and especially in the thalamus, hippocampus, entorhinal cortex and sensorimotor cortex. These distribution studies in conjunction with previous neuropharmacological studies, support the concept that adenine nucleotides may function as intercellular mediators in various regions of the brain.     

The subcellular distribution of lysergic acid diethylamide in the rat brain

Résumé Quinze min après une injection i.p. de LSD, près de 48% de la drogue présente dans le cerveau fut trouvée dans le surnageant, 20% dans la fraction nucléaire, 15% dans la fraction synaptosomale, 6% dans la fraction microsomale, 5% dans la fraction myélinique et près de 1,7% dans la fraction mitochondriale. Les fractions synaptosomale et mitochondriale sont presque saturées en LSD lors d'une injection de 10 mg/kg de LSD.

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Acknowledgment. I thank Dr.J. Knopp for his help with the spectrophotoflourometric analysis.     

Regional cyclic AMP levels in homogenates of rat brain after ketalar and trifluoperazine

There was a significant fall in cAMP levels after administration of TFP or ketalar. Different amounts of cAMP were present in different regions of rat brain. Concentrations of cAMP in different regions of the rat brain were found to decrease in the following order: cerebrum > thalamus with hypothalamus > midbrain > hippocampus > cerebral cortex.     

Regional cyclic AMP levels in homogenates of rat brain after ketalar and trifluoperazine

Summary There was a significant fall in cAMP levels after administration of TFP or ketalar. Different amounts of cAMP were present in different regions of rat brain. Concentrations of cAMP in different regions of the rat brain were found to decrease in the following order: cerebrum > thalamus with hypothalamus > midbrain > hippocampus > cerebral cortex.Conducted under ocntract No 10.4.2 with the Polish Academy of Science.     

Regional distribution of pancreatic polypeptide cells in the 21-day fetal rat pancreas

Summary 21-day fetal rat pancreata were stained with the unlabeled antibody peroxidase-antiperoxidase technique using bovine pancreatic polypeptide as the primary antibody. Total counts of pancreatic polypeptide cells were made over the entire pancreas. It was found that the head region contained the greatest number of pancreatic polypeptide cells with the body next and the tail having the smallest number. The pancreatic polypeptide cells of the body were concentrated in the portion closest to the distal duodenum. This distribution pattern seems to support the suggested role of pancreatic polypeptide on the physiological function of the digestive tract.The authors wish to express their appreciation to Dr R. McEvoy, T. Whittlsey, G. Wassilchenko and D. Wilson for their assistance in this study. To whom reprint requests should be addressed.     

Regional distribution of blood flow in the renal cortex

Zusammenfassung Es wird ein autoradiographisches Verfahren zur Bestimmung der regionalen Durchblutungsverteilung in differenziert strukturierten Organen beschrieben. Mit dieser neuen Methode wird die Durchblutung der Nierenrinde untersucht. Es wird nachgewiesen, dass die Durchblutungs- bzw. Widerstandsverteilung innerhalb der Rinde nicht einheitlich ist, sondern dass sich drei verschieden durchblutete, anatomisch voneinander abgrenzbare Teile unterscheiden lassen.     

Regional variations and particle binding of free amino acids in the rat brain: effects of amphetamine and methaqualone]

Amphetamine and methaqualone affected aspartate level in various regions of Rat brain. Increased aspartate level in cerebral cortex of methaqualone treated Rats was produced in the aspartate fraction whose binding to subcellular particules was Na+ dependant. This work suggest that aspartate level was altered only in brain fraction implicated in the pharmacological effects of the drugs.