尾巨桉化感作用对广金钱草化学成分的影响

【目的】研究尾巨桉Eucalyptus urophylla×Eucalyptus grandis对广金钱草Desmodium styracifolium(Osb.)Merr.药材化学成分的影响,探索尾巨桉-广金钱草复合经营模式的可行性。【方法】在试验田中,以不同类型和重量的尾巨桉落叶铺盖在土壤上并种植广金钱草,同时另在尾巨桉人工林中直接套种广金钱草,待药材成熟后统计其产量,并利用HPLC指纹图谱法及含量测定法检测其化学成分变化。【结果】广金钱草在尾巨桉林下种植时,产量稍有下降,但药材指纹图谱与正常种植无显著差异。【结论】广金钱草可以套种在尾巨桉人工林下。     

绿桐增殖、生根培养及炼苗移栽研究

本研究旨在建立适于绿桐（Paulownia fortunei×Paulownia tomentosa）的快速无性繁殖技术,为试管苗工厂化生产提供技术参考。实验以绿桐当年生枝条为外植体,对消毒方式、取材部位、植物生长调节剂及其浓度配比条件进行优化,筛选合适的诱导、增殖和生根培养基。实验结果表明,最佳消毒方式为依次采用75%乙醇、等体积的10%次氯酸钠与0.1%升汞混合液、0.1%升汞消毒;当年生嫩条顶端10 cm长度的茎段为最佳外植体;外植体在MS+1.0 mg/L 6-苄氨基嘌呤（6-BA）+1.0 mg/L 2,4-对氯苯氧乙酸（2,4-D）+0.8 mg/L赤霉素（GA₃）的培养基中,能诱导出长势健壮的不定芽;在MS+0.8 mg/L 6-BA+0.4 mg/L萘乙酸（NAA）的增殖培养基中,增殖系数达2.02;而生根以1/2 MS+0.6 mg/L吲哚-3-丁酸（IBA）+0.4 mg/L NAA培养基为最佳,生根率达100%;对组培苗进行炼苗后覆膜保湿,移栽存活率可达92%。本研究获得了适合绿桐快速繁殖的培养基,建立了一套绿桐快速繁殖体系。     

巨尾桉与黧蒴栲、红锥光响应曲线的比较研究

【目的】通过对广西桂林灵田乡巨尾桉（Eucalyptus grandis×E．urophylla）、黧蒴栲（Castanopsis fiSSa）和红锥（Castanopsis hystrix）光合一光响应曲线研究,寻找高光效阔叶树种。【方法】采用Li-6400便携式光合仪测定各树种光响应曲线。【结果】当光合有效辐射（PAR）开始增强时,各树种问净光合速率（Pn）差异较小,但随着光合有效辐射强度的增加,不同树种问净光合速率出现较大的差异。各树种的净光合速率随着光合有效辐射升高达到最高点之后稳定。蒸腾速率（Tr）、气孔导度（Gs）、水分利用效率（WUE）和CO2利用效率（CuE）对光合有效辐射的响应趋势与净光合速率相似,胞问CO：浓度、光能利用效率对光合有效辐射的相应趋势则与净光合速率相反。3个树种的净光合速率为3．975～20．800／μmol·m^2·s^1,巨尾桉最高,黧蒴栲最低。光补偿点和光饱和点都是巨尾桉最高,黧蒴栲最低。【结论】综合各指标和光响应变化规律,确定巨尾桉为优良的高光效阔叶树种,可在水分和光照条件好的地方适当推广。     

Enhancive effect of N,N′-dinitrosopiperazine on inducing precancerous lesion on nasal and/or nasopharyngeal epithelia of TgN(p53mt-LMP1)/HT mice

Objective: To investigate the enhancive effect of N,N′-dinitrosopiperazine (DNP) on induced carcinogenesis in nasal and/or nasopharyngeal epithelia among TgN(p53mt-LMP1)/HT transgenic mice to examine the underlying mechanism for the development of nasopharyngeal carcinoma (NPC). Methods: TgN(p53mt-LMP1)/HT transgenic mice and the same strain of C₅₇BL/6J wild-type mice both at the age of 5 months were randomly divided into 2 groups in parallel, respectively, i.e., TgN(p53mt-LMP1)/HT cancerous lesion-inducing group (TI), TgN(p53mt-LMP1)/HT control group (TC), C₅₇BL/6J cancerous lesion-inducing group (CI), and C₅₇BL/6J control group (CC). TI and CI mice were treated only with DNP for 16 weeks, twice each week, while TC and CC mice were given the same volume of saline as controls. At the end of treatment, animals were sacrificed to collect epithelial tissue samples from nasal cavity and nasopharynx for pathohistological evaluation by haematoxylin and eosin (HE) staining and for determination on the expression of TRAF2, c-Jun, and p16 by immunohistochemistry. Results: Atypical hyperplasia was more significant in the samples of TI than in those of TC, CI, and CC, with the rates of lesions being 90%, 10%, 0, and 0 (P<0.01) respectively, though DNP was used alone in a much shortened inducing period at less dosage and without the use of carcinogenic promoter 12-O-tetradecanoylphorbol-13-acetate as usual. The expressions of tumor necrosis factor (TNF) receptor-associated factor 2 (TRAF2) and c-Jun in these samples were significantly up-regulated in TI (P<0.01), while the expression of p16 was significantly lower in TI than in the other groups (P<0.01). Conclusion: TgN(p53mt-LMP1)/HT mice hold inherited constitutional defect in immune surveillance function, which can be aggravated by environmental carcinogens, such as DNP used even though in a much less strength. The enhanced carcinogenesis-inducing effect of DNP on TgN(p53mt-LMP1)/HT mice should be closely associated with abnormal signaling of activator protein-1 (AP-1) pathway, especially up-regulated expressions of TRAF2 and c-Jun, and down-regulated expression of p16.     

用第一性原理研究Co2Ti1-xMxAl(x=0,1/4;M=C,Zr)晶体的电子结构

用广义梯度近似密度泛函和全势能线性缀加平面波方法研究了晶体Co2Til-xMxA1（x=0,1/4;M=C,Zr）的电子结构。为了更好的了解杂质元素对晶体A1Co2Ti结构的影响,对杂质元素M（M=C,Zr）替代晶体A1Co2Ti中的Ti（1／2,1／2,1／2）位进行了对比研究。同时,对杂质元素M（M=C,Zr）对晶体A1Co2Ti的品格常数、体弹模量和原子之间的化学键产生的作用进行了探讨。