首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 390 毫秒
1.
林曦碧 《武夷科学》2008,24(1):78-81
通过比较4株白僵菌菌株的产孢量及其对油茶毒蛾的致病力,结果发现白僵菌菌株Bf08,B01,产孢量分别为(5.1±0.4)×10^8,(3.4±0.4)×10^8孢子/cm^2,接种油茶毒蛾后其巩。分别为5.64,6.62d,说明对这两个菌株油茶毒蛾的毒力很强,是可用于油茶毒蛾防治的优良白僵菌菌株。  相似文献   

2.
对柞栎象成虫和幼虫虫体的白僵菌菌株进行分离、鉴定及生物学特性观察.结果表明:柞栎象体上的虫生真菌为布氏白僵菌;影响布氏白僵菌菌落直径生长量的3个因素中菌株种类居于首位,其次是培养温度;影响产孢量的首要因素是培养基种类,其次是培养温度,最后是菌株种类.对柞栎象成虫提取的布氏白僵菌产孢条件进行选择,结果表明:20~25℃时,在PPDA或PDA培养基上培养可获得最大产孢量.在柞栎象成虫和幼虫虫体上提取的布氏白僵菌萌发率相似,分别为89.11%和85.67%;柞栎象成虫、幼虫虫体提取的布氏白僵菌菌株的抗逆性表现为随着照射时间的增加,生长速度减慢.以紫外线照射45 min为分界点,照射时间小于45 min时柞栎象幼虫菌株长势强于成虫菌株,大于45 min时成虫菌株强于幼虫菌株.  相似文献   

3.
在室内对球孢白僵菌(Beauveria bassiana) 6个自然菌株的营养生长、产孢量、孢子活力、 孢子耐热能力、孢子抗紫外线能力及其对萧氏松茎象成虫的毒力等指标进行了研究。结果表明:菌 株B6和B3对萧氏松茎象成虫的毒力最强,20 d的死亡率和白僵率均达到90 %和80 %。B6菌株生长最 快,14 d直径生长量达24.21 mm,产孢量最高,达12.15×107个/mm2,产孢时间最早,108 h就开始 产孢。B3菌株的孢子萌发率最高,36 h后达到71.07 %,且耐热性较好,抗紫外线能力较强,但其在 PPDA培养基上的产孢量最低。因此,B6菌株是防治萧氏松茎象的优良菌株,具有较大应用价值。  相似文献   

4.
对采自安徽黄山的松墨天牛Monochamus alternatus成虫虫体的病原真菌进行分离与鉴定,共获得8个球孢白僵菌Beauveria bassiana菌株,对其菌落形态、菌株生长速度、产孢量和萌发率进行了研究.最终成功筛选出了球孢白僵菌Bb660菌株,该菌株具有生长速度快、萌发率高和产孢能力强等生物学特性,在松墨天牛的生物防治中将有潜在的应用价值.  相似文献   

5.
柳杉毛虫高毒力白僵菌Bbd3菌株的筛选及应用   总被引:3,自引:0,他引:3  
对天然柳杉毛虫僵虫组织分离纯化,选取8株菌株在PDA 虫尸培养基上培养,在比较菌粉质量以及不同菌株室内外毒力测定试验基础上,筛选出菌丝生长快、产孢时间短、产孢量高、对柳杉毛虫毒力强的2株Bbd3和Bbd6高毒力白僵菌优良菌株,将具最优品质的Bbd3菌株大规模生产和大面积防治应用,菌粉平均含孢量达3.09×1010个/g,平均孢子萌发率达99.1%,平均防治效果达92%以上。  相似文献   

6.
几种复合微生物杀虫剂对刚竹毒蛾的药效试验   总被引:12,自引:1,他引:12  
应用3种白僵菌菌株、苏云金杆菌分别对2 ̄3龄刚竹毒蛾幼虫进行毒力测定,结果表明,4种生物制剂的毒力效果由高到低分别为白僵菌松毛虫菌株(永安)〉苏云金杆菌〉白僵菌刚竹毒蛾菌株(邵武)〉白僵菌松毛虫菌株(邵武)。应用白僵菌+苏云金杆菌+低浓度的80%敌敌畏复合杀虫剂对刚竹毒蛾2 ̄3龄及5 ̄6龄幼虫进行正交试验,结果表明,复合杀虫剂的效果均高于单一生物制剂,其最佳组合为白僵菌松毛虫菌株(永安)孢子浓度为  相似文献   

7.
利用PDA培养基,对收集自广东茂名的玉米螟(Pyrausta nubilalis)病原真菌(编号:Bba1菌株)进行分离培养。对Bba1菌株的菌落形态、产孢结构和分生孢子形态、大小进行观察和测量,从形态学初步鉴定该菌株为球孢白僵菌(Beauveria bassiana);同时对Bba1菌株的26,s r DNA和r DNA ITS区域进行序列分析,与NCBI数据库中球孢白僵菌的同源性达到99%,以上。最终判定来自玉米螟的病原真菌Bba1菌株为球孢白僵菌(Beauveria bassiana)。  相似文献   

8.
球孢白僵菌Bb051230菌株对番石榴实蝇的毒力测定   总被引:1,自引:0,他引:1  
利用球孢白僵菌Bb051230菌株不同孢子液浓度对番石榴实蝇成虫、幼虫和蛹进行毒力测定,结果表明:在4.0×108个/mL浓度下第8天成虫的累计校正死亡率为(89.66±5.34)%,致死中时间(LT50)(4.53±0.31)d,第8天的致死中浓度(LC50)为(2.687 2±0.325 8)×105个/mL;4.0×108个/mL浓度下第8天幼虫的累计校正死亡率为(91.83±4.02)%,致死中时间(LT50)(4.66±0.59)d,第8天的致死中浓度(LC50)为(2.329 7±0.418 5)×105个/mL;4.0×108个/mL浓度下第8天蛹的累计校正死亡率为(83.79±2.27)%,致死中时间(LT50)(4.89±0.44)d,第8天的致死中浓度(LC50)为(2.829 1±0.156 3)×105个/mL.  相似文献   

9.
木毒蛾白僵菌优良菌株的自然筛选及应用研究   总被引:3,自引:0,他引:3  
本研究通过对林间采集的天然木毒蛾僵虫蛹进行组织分离和单孢分离纯化 ,选取 12个典型单孢菌株在马铃薯虫尸琼脂培养基上的表现性状以及与不同菌株的室内外毒力测定试验比较 ,从中筛选出 Bm1-1、Bm2 - 7、Bm3- 3三个木毒蛾白僵菌优良菌株 ,并将最具优良品质的 Bm1- 1菌株进行大量生产和大面积应用防治示范 ,取得了显著的效果  相似文献   

10.
研究了温度对绿僵菌菌株LA06、LD04生物学特性的影响,结果表明两菌株生长和产孢的适宜温度均在25-30℃;适温下LA06在第3d开始产孢,产孢量达6×107个/cm2,LD04则第7d才开始产孢,产孢量仅1×107个/cm2;两菌株均以30℃下孢子萌发最快,GT50分别为7.55 h和9.88 h;45℃下处理8 h后LA06孢子萌发率为45.35%,LD04则为0.LA06菌株在实际应用中具有较大潜力.  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

13.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

14.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

15.
正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-  相似文献   

16.
We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-  相似文献   

17.
正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that  相似文献   

18.
For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the  相似文献   

19.
<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with  相似文献   

20.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号