凤尾菇发酵液抑制枇杷炭疽病菌组分的分离及活性研究

以枇杷炭疽病菌为靶标菌,测定了凤尾菇发酵液的乙酸乙酯和正丁醇萃取物的抑菌作用,对抑菌活性较强的乙酸乙酯萃取物采用硅胶柱和Sephadex LH-20凝胶柱进行分离.结果表明,经硅胶柱分离后获得5个组分,其中F4组分对枇杷炭疽病菌有较强的抑菌活性,F4组分再经Sephadex LH-20凝胶柱进一步分离获得3个组分,其中F4-A组分对枇杷炭疽病菌抑菌活性最强.电镜观察结果显示,经F4-A组分处理后的枇杷炭疽病菌菌丝呈不规则的肿大和缢缩,菌丝细胞壁不规则增厚,细胞原生质不均匀,有较大的空腔形成,囊泡增多,这些变化最终导致菌丝细胞解体死亡.     

紫菜多糖的提取及抗流感病毒活性研究

采用4种方法提取坛紫菜多糖,其中综合法提取率高达16.58%.气相色谱及化学分析表明其单糖主要由半乳糖、3,6-内醚-L-半乳糖、甘露糖和少量木糖组成.可溶性多糖经DEAE-23柱层析进一步纯化,得到蒸馏水洗脱组分———中性多糖和1.0-2.0mol LNaCl梯度洗脱组分———酸性多糖,各占4.12%和95.88%.酸性多糖经硫酸降解和超滤膜分离得到多糖组分F1(350030000),二者比例为4∶1.鸡胚实验表明,紫菜多糖具有抗甲1型流感病毒活性,且F1>F2>中性多糖.     

利用离子色谱测定海带硫酸多糖中硫酸根的含量

建立了用离子色谱测定海带硫酸多糖硫酸根的方法。海带经酶解，季胺盐沉淀，离子交换和凝胶过滤，从中提取分离到3种多糖：F1，F2，F3，在对3个组分进行了GPC及红外光谱分析，证明匀为均一硫酸多糖的基础上，2组分分别经灰化，定容处理后，利用离子色谱对它们的硫酸根含量分别进行了测定，结果分别为：F1：28.41KD,F2:15.87KD,F3:32.50KD。与传统硫酸钡浊度法相比，该方法消除了其它离子及杂质的干扰，操作简单，具有精确，灵敏，高效等许多优点，是测定硫酸多糖硫酸根含量的理想方法。     

CP破坏与超对称模型

分析CP破坏与超对称模型,然后由超对称模型出发,认为u.d夸克有两类反夸克:F erm i型和B ose型反夸克,质子P F 1也有两类反质子P -F 1及P -B 1,且P -B 1=P -F 1 3 lS0,F([q1q2]g).当P F 1P -B 1对湮灭时,只有P -B 1中的P -F 1与P F 1被湮灭而转化的光子,l0S,F([q1q2]g)组分将被保留下来.q1,q2,g等亚夸克都只是正物质的组分.因此,宇宙中正物质应多于反物质.     

基于试验拟合的泡沫油油藏模拟模型评价及渗流机制研究

建立能够反映泡沫油渗流机制的4组分(水、原油、溶解气和自由气)和5组分(水、原油、溶解气、分散气和自由气)模型,并以黑油模型为参照,通过拟合压力衰竭试验数据的方法,系统评价这3种模型的适用性,分析反应因子(代表气泡聚集和增长的速度)F1、原油黏度μo、压力控制时间t P等参数对泡沫油流的影响。结果表明:5组分泡沫油模型更适用于泡沫油油藏的模拟;F1、μo和t P对泡沫油模型影响较大;随着F1的增大,4组分泡沫油模型累积产油量减少,累积产气量增加,而5组分泡沫油模型累积产油、气量均增加;随着μo增大,3种模型累积产油、气量均减少;加快压力衰竭速度使临界含气饱和度变大,气相流动能力降低,泡沫油溶解气驱开发效果变好。     

阴沟肠杆菌(Enterbacter Cloacae)糖蛋白活性组分抑瘤效应的研究

从水体中分离到阴沟肠杆菌（Enterbacter Cloacae)，利用该菌制剂对荷肉瘤S180（实体型）小鼠有抑瘤作用，该菌大量培养后，将菌体超声波破碎，十六烷基三甲基溴化铵（Cetyltrimethylaminonium bromide,CTAB)沉淀，乙醇沉淀得粗提物，再经DEAE纤维素柱层析，分步收集，聚乙二醇浓缩得F1、F2、F3和F4 4种主要组分，形态观察法体外抑瘤实验证明F2、F3组分对肝癌QGY7703细胞有明显杀伤作用，F3作用显著，MTT法检测F3对肝癌QGY7703，肺腺癌A549，胃癌KatoⅢ，肠癌SW1116瘤细胞株均有明显杀伤作用，其IC50分别为0．024，0．0123，0．0035，0.012mg/mL。体外抑瘤试验证明F3对小鼠无急性毒作用，并且对小鼠Lewi肺癌有抑制作用，蛋白电泳，红外和核磁共振表明F3是含木糖成分的糖蛋白，其相对分子质量为38ku.     

负载型Sb催化剂在Baeyer—Villiger反应中的应用研究

采用浸渍法制备了新型负载型SbR／SBA-15及SbF3／F—SBA-15非均相催化剂,考察了不同载体、活性组分的负载量等对金刚烷酮Baeyer-Villiger氧化反应的影响,同时对反应条件进行了优化．当反应温度为70℃,反应7h,反应底物与催化剂最佳摩尔比为1：0．4时,在系列具有不同sbR负载量的SbR／F—SBA-15催化剂中,10％-sbR／F—SBA-15表现出最佳的催化活性．结合XRD以及BET结果,其活性较高的原因主要归因于载体SBA-15中修饰的F离子能够与活性组分sbR发生相互作用,从而有效提高10％-SbR／F—SBA-15中活性组分与载体的相互作用,进而提高其分散度．     

双通道气相色谱-脉冲氦离子化检测器对SF_6电力设备中17种气体组分的检测

基于SF6等气体组分分析在电气设备故障/缺陷诊断中的显著作用,采用双通道气相色谱-脉冲氦离子化检测器(GC-PDHID)建立了SF6电力设备中17种常见的气体组分(CO、CF4、CH4、CO2、C2F6、C2H2、COS、C2H4、C2H8、H2S、SO2F2、C3F8、C3H6、C3H8、CS2、SO2、S2OF10)的定性定量分析方法。结果发现,通过采用双通道气相色谱的方法将不同的气体组分切换到由0.5 nm柱和Poropak Q柱组成的通道A以及由HP-5毛细管柱组成的通道B,17种气体中除了C2H4与C2H6、C3H6与C3H8无法分离外,其余各组分均得到的良好的分离。同时,PDHID对这17种气体进行检测,结果表明,17种气体的峰面积的相对标准偏差(RSD)小于7%,检出限(LOD)为10-8(SO2F2)~10-6(CO2)。该方法重现性好,灵敏度高,适用于对该15种气体进行定性定量分析。用以上的方法对气体绝缘开关设备(GIS)中气体组分进行分析,检测到N2、O2、CO2、CF4、SO2、SO2F2等。其中,SO2和SO2F2的检出说明该GIS设备内可能发生了火花放电。     

Pr~(3+)掺杂Ge-Ga-S-KCl硫卤玻璃的近红外发光研究

采用熔融淬冷法制备掺Pr3+(0.1%,0.3%,0.5%,0.7%)(按物质的量计算百分数,下同)的0.64GeS2-0.16Ga2S3-0.2KCl硫卤玻璃.测试玻璃的光学吸收谱和光致发光谱,在红外吸收谱中有4个明显的吸收峰,分别是Pr3+的基态3H4向3F4,3F3,3F2和3H6激发态跃迁引起的吸收.近红外区的3个发光峰,分别对应1D2→1G4(~1 390 nm),3F4→3H4(~1 430 nm)和3F3→3H4(~1 480 nm)的跃迁.0.5l%PrCl3掺杂时该组分玻璃的发光强度最高,而且吸收峰和发光峰峰值位置比相应的硫系玻璃有蓝移.分析表明:吸收峰和发光峰的蓝移是由于硫卤玻璃的离子键性比相应的硫系玻璃的离子键性强,导致Pr3+的相应能级间隔增大.     

Cartan型阶化李代数的中心扩张和H~1(L,L)

A.S.Dzhumadil'daev决定了Cartan型阶化李代数的上同调群H~2(L,F)的结构,其中L=W(1,m)(p≥3),S(3,m)(p≥3),H(n,m)(p>3),K(n+1,m)(n≡-3 mod(p)和p≥3)和F是特征数p的代数闭域,R.Farnsteiner决定了H~2(L,F)的结构,其中L=W(n,m)(p>3),S(n,m)(p>3和n=3),H(n,m)(p>3)和K(n,m)(p>3)。利用H~2(L,F),他们也得到相应的中心扩张     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that