A highly active glutathione peroxidase in red blood cells of normal and acatalasemic mice

Summary The activities of catalase (E.C.1.11.1.6) and glutathione peroxidase (E.C.1.11.1.9) were compared in red blood cells from humans, ducks and normal and acatalasemic mice. In the cells from both strains of mice, an equally high activity of GSH-Px was found which could be inhibited completely by iodoacetate but was not sensitive to N-ethylmaleimide.Acknowledgment. This work was supported by a postdoctoral fellowship from the Gesellschaft für Strahlen- und Umwelt-forschung, München, Federal Republic of Germany, and was performed under contract with the U. S. Energy Research and Development Administration at the University of Rochester Biomedical and Environmental Research Project and has been assigned Report. No. UR-3490-1138.     

A highly active glutathione peroxidase in red blood cells of normal and acatalasemic mice

The activities of catalase (E.C.1.11.1.6) and glutathione peroxidase (E.C.1.11.1.9) were compared in red blood cells from humans, ducks and normal and acatalasemic mice. In the cells from both strains of mice, an equally high activity of GSH-Px was found which could be inhibited completely by iodoacetate but was not sensitive to N-ethylmaleimide.     

The investigation ofXenopus laevis hemoglobins during development by a fluorescent antibody

Zusammenfassung BeiXenopus laevis findet man im Kaulquappenstadium 2 fötale Hämoglobine (HbF) und nach der Metamorphose 3 neue Hämoglobine (HbA) vom Erwachsenentypus. Immunofluoreszenz-Untersuchungen lassen erkennen, dass während der Metamorphose keine oder nur wenige Erythrozyten gleichzeitig HbF und HbA enthalten.     

Catalase inhibition by potassium chlorate in red cells

Zusammenfassung Die Geschwindigkeit der Katalaseaktivitätsverminderung bei mit Kaliumchlorat vergifteten Erythrozyten wird vom pH beeinflusst. Die vorgeschlagene Methode ist zur Gewinnung von Erythrozyten niedriger Katalaseaktivität geeignet.     

Loss of antibody production accompanied by chromosome loss in a cloned hybrid line secreting antibodies to sheep red blood cells

Somatic cell hybrids between Sp2/O-Ag14 mouse myeloma cells and lymphocytes derived from BALB/c mice hyperimmunized with sheep red blood cells (SRBC) were produced. One hybrid producing IgG1 antibody to SRBC was selected, cloned twice and subsequently transferred to BALB/c mice. After a number of transfers it was found that the antibody titer in ascites fluid gradually decreased. Cytogenetic analysis revealed gradual chromosome loss in the hybrid clone, which produced progressively less antibody.     

Loss of antibody production accompanied by chromosome loss in a cloned hybrid line secreting antibodies to sheep red blood cells

Summary Somatic cell hybrids between Sp2/O-Ag14 mouse myeloma cells and lymphocytes derived from BALB/c mice hyperimmunized with sheep red blood cells (SRBC) were produced. One hybrid producing IgG₁ antibody to SRBC was selected, cloned twice and subsequently transferred to BALB/c mice. After a number of transfers it was found that the antibody titer in ascitec fluid gradually decreased. Cytogenetic analysis revealed gradual chromosome loss in the hybrid clone, which produced progressively less antibody.     

The localization of water-soluble proteins in the wheat endosperm as revealed by fluorescent antibody techniques

Zusammenfassung Die Anreicherung wasserlöslicher Proteine in Endospermzellen von Weizenkörnern, in der die Stärkegranula umgebenden Zone, konnten mittels der Fluoreszenz-Antikörper-Technik nachgewiesen werden.     

Reduction of ferricytochrome c by human red cells

Summary Human red cells reduced extracellular ferricytochrome c to ferrocytochrome c under various conditions, suggesting that ferricytochrome c reducing systems are present at the outer surface of the red cell membrane.     

Reduction of ferricytochrome c by human red cells

Human red cells reduced extracellular ferricytochrome c to ferrocytochrome c under various conditions, suggesting that ferricytochrome c reducing systems are present at the outer surface of the red cell membrane.     

The influence ofBordetella pertussis on the kinetics of antibody production to sheep red blood cells in NMRI mice

Zusammenfassung Die simultane i.p. Injektion von 4 × 10⁸ Schaferythrozyten und 2 × 10⁹ Zellen vonB. pertussis führt im Vergleich zur alleinigen Injektion des Erythrozytenantigens bei NMRI-Mäusen zu einer gesteigerten und verlängerten Proliferation M- und G/A-Antikörper produzierender Milzzellen.

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This work was supported by research grant No. Fi 115/1 of the Deutsche Forschungsgemeinschaft.     

Immunofluorescent technique to detect anti-penicillin antibody

Resumen Se propone un sencillo y rápido método de inmuno fluorescencia para detectar anticuerpos antipenicilina: Frotis de eritrocitos de conejo, sobre los que se han fijado moleculas de peniciloyl-polylisina, son tratados con suero de conejo inmunizado y despues con suero de cabra anticonejo marcado con isothiocianato de fluoresceina. De este modo se observa acusada fluorescencia en los casos positivos.     

Demonstration of cell-bound antibody by fluorescent microscopy in the intestine of rabbits following live enterai cholera vaccination

Zusammenfassung Mit Hilfe der Fluoreszenzmikroskopie lassen sich zellgebundene Immunkörper in isolierten Darmepithelzellen erwachsener Kaninchen nach Darmimpfung mit lebender Kultur desVibrio-eltor-Stammes erkennen. Immunkörper waren in den Epithelzellen nicht immunisierter Kaninchen nicht nachweisbar. Es wird die mögliche Rolle der zellgebundenen Immunkörper beim antibakteriellen Immunmechanismus (Schutz gegen Cholera und Ansteckungsort) diskutiert.