On the mineralocorticoid and hypertensogenic properties of 16β-Hydroxy-Dehydroepiandrosterone

Summary Dosages of either 1 or 2 mg daily of 16-hydroxy-dehydroepiandrosterone, given to mononephretomized, salt-loaded female rats, had no detectable effect upon saline consumption, blood pressure, kallikrein excretion or heart and kidney weight. Its alleged mineralocorticoid properties, as judged by these criteria, were not demonstrable.This study was supported by a grant No. HL 15319 from the United States Public Health Service.     

Effect of aldosterone and methylprednisolone on cardiac NaK-ATPase

Summary Aldosterone (15 g BID) and methylprednisolone (8 mg QD) administration to female guinea-pigs augmented both the total and the specific activity of NaK-ATPase but not the activity of adenylate cyclase in the cardiac sarcolemma. The rise in NaK-ATPase was due to increase in the number of enzyme molecules; catalytic activity and ouabain-sensitivity of individual molecules did not change.Acknowledgments. This work was supported by grant 1 R01 HL16611 from the National Heart and Lung Institute of the National Institutes of Health, United States Public Health Service. I thank Mr Kooil Kang for his excellent technical assistance.     

Continuous quantitative recording of changes in vascular permeability

Zusammenfassung Es wird die Anwendung radioaktiven Phosphanilsäure-Rinderserum-Albumins zur kontinuierlichen Messung von Änderungen der Gefässwandpermeabilität beschrieben.

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This work was supported by a grant and Fellowship (to A.B.) from the Asthma Foundation of New South Wales and a grant from the United States Public Health Service (Grant No. HE 08513-02).     

Degradation of [3H]thymidine by a pentosyltransferase (EC 2.4.2.4) in the plasma of man and different animals

Summary [³H]Thymidine is degraded by an enzyme (thymidine phosphorylase; EC 2.4.2.4) which we have identified in the plasma of man and some animals. The presence of this enzyme in plasma or sera used to supplement culture media may, under certain experimental conditions, limit the validity of measuring the uptake of radiolabeled thymidine as a means of defining DNA synthesis.Supported in part by United States Public Health Service grant CA-17658 from the National Cancer Institute, and grant RR-05648 from the National Institutes of Health.     

Thein vivo effect of some peroxides upon the mouse ascites carcinoma of ehrlich

Zusammenfassung Die Lebensdauer von mit Ehrlich-Ascites-Karzinom injizierten Mäusen wird durch Wasserstoffsuperoxyd und Harnstoffperoxyd auffallend verlängert. Der Tumor wird dabei von der Ascites- in die solide Form umgewandelt.

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This work was supported by a grant from the United States Public Health Service (C-3538).     

Structural alterations in normal and axotomized facial nucleus neurons after treatment with morphine

Summary Chronic morphine administration induced ultrastructural alterations in neurons of the facial nucleus and increased the incidence of cell death after axotomy. These findings may correlate with the significant depression of neuronal metabolism known to occur after opiate treatment.Supported by a grant from the United States Public Health Service, NIDA 5 RO1 DA 0014-06.     

The visual response of the purple shore crab,Hemigrapsus nudus,to ionizing and non-ionizing radiations

Zusammenfassung Im Krabbenauge vonHemigrapsus nudus entstehen elektroretinographische Reaktionen (ERG) mit sichtbarem Licht und Röntgenstrahlung, die andeuten, dass dieselben Rhodopsin-Erregunsprozesse mit beiden Strahlungsarten stattfinden. Nach⁹⁰Sr--Strahlung wird angenommen, dass Cerenkov-Effekt- und induzierte Fluoreszenz-Lichterzeugung die-ERG-Reaktion deutlich beeinflussen.

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This research was conducted under a grant from the Environmental Control Administration, CPEHS, U.S. Public Health Service No. EC00119.

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Public Health Service Radiological Health Fellowship No. 97996-06-69.     

Vitamin A antibodies: Application to radioimmunoassay

Summary A radioimmunoassay for serum vitamin A is described which can detect as little as 1 ng of retinol. The statistical characteristics of this assay are presented and its use in a nutritional experiment is discussed.This work was supported by United States Public Health Service grant 2 RO1 AM19716.     

Lack of major cytoplasmic protein contamination of rat liver nuclear chromatin

Résumé Des mesures quantitatives et des modèles sur gels d'acrilamide ont permi de conclure que les protéines cytoplasmiques préparées de cette manière ne produisent pas de contamination appréciable.

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Supported by grant No. CA-07746 from the United States Public Health Service and grant No. G-138 from the Robert A. Welch Foundation.     

Effect of angiotensin II on neonatal lamb carotid arteries

Summary Isometric tension was measured in arterial strips from neonatal lambs and adult sheep, after stimulation by angiotensin II. During the early maturation period immediately following birth (3 weeks) there was a progressive increase in sensitivity to the agent.This study was supported by the Golden Empire Chapter of the American Heart Association and the United States Public Health Service, grant No. PHS HL 14780-03.     

Anamnestic response to an opioid

Zusammenfassung In Mäusen entsteht eine Toleranz gegen den kataraktogenen Effekt einer Einzeldosis von Opioid. Werden fraktionierte Dosen gegeben, so entwickelt sich jedoch eine weitaus grössere Toleranz, wofür eine Sensibilisierung als Ursache angenommen wird.

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Supported by a grant No. MH-12988-05 from the United States Public Health Service.     

Effect of cocaine and tyramine on noradrenaline release and uptake in isolated nerve vesicles

Zusammenfassung Die Ergebnisse sprechen nicht dafür, dass die durch Cocain bedingte Hemmung der Noradrenalinaufnahme an die Granula gebunden ist. Es ist anzunchmen, dass diese vielmehr an die Axonmembrane verlegt ist.

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This work has been supported by grant NA 04432-02 from the United States Public Health Service and by the Swedish Medical Research Council; Project No. 14X-97-01.     

Compound 48/80 decreases adenosine 3′5′-monophosphate formation in rat peritoneal mast cells

Zusammenfassung Präparat 48/80 unterdrückt die Bildung von 3,5-AMP aus Adenin in isolierten peritonealen Mastzellen. Es konnte keine zeitliche Verschiebung zwischen dieser Unterdrückung und der Histamin freisetzenden Wirkung von Präparat 48/80 festgestellt werden.

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This investigation was supported by United States Public Health Service Grant GM 13016 from the National Institute of General Medical Sciences.     

The detection of superoxide anion from the reaction of oxyhemoglobin and phenylhydrazine using EPR spectroscopy

Summary The low temperature EPR spectrum of a quickly reacted mixture of oxyhemoglobin and phenylhydrazine was studied. With the use of a computer, the spectral contribution of methemoglobin in the region of g=2 was subtracted. The remaining spectrum was that of an axial free radical (g=2.00, g=2.06) having the magnetic parameters of superoxide anion. In the presence of superoxide dismutase, this axial radical was not seen, confirming that superoxide anion is indeed generated by the reaction.The portion of this investigation carried out at the Albert Einstein College of Medicine was supported in part by US Public Health Service Research Grant HL-93399 from the Heart and Lung Institute and by National Institute Contract Nol-CP-55606 to J.P. This is communication No. 378 from the Joan and Lester Avnet Institute of Molecular Biology.Predoctoral fellow in the Medical Scientist Training Program (United States Public Health Service Grant 5-TO-5-GM 01669-12) at the New York University School of Medicine.Recipient of a grant-in-aid from the New York Heart Association.     

The effects of various agents in vitro on homocarnosine-carnosine synthetase from rat brain

Summary The present paper reports the first evidence that homocarnosine-carnosine synthetase from rat brain requires free sulfhydryl groups for activity. The activity of the synthetase can be stabilized by dithioerythritol and inhibited strongly by Cu²⁺, Cd²⁺, Hg²⁺, Zn²⁺, and to a lesser extent by Ca²⁺, Ni²⁺, Li, chlorpromazine, -methyl DOPA and norepinephrine at the concentrations tested.Acknowledgment. This work was supported by United States Public Health Service Grant No. NS-06137.     

Experimental hypertension elicited by injections of methyl cellulose

Résumé Des injections sous-cutanés d'une solution de méthyl-cellulose provoquent dans la rate une vraie hypertension artérielle maligne. Les animaux utilisés eurent fréquemment des dèmes et des ascites et toujours des lésions artérielles dans le cur, les reins et ailleurs.

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This study was supported by grants H-2703 and H-4327 from the United States Public Health Service.     

Trace element stimulation of keratin (Hair) degradation by oral keratinolytic microflora

Zusammenfassung Nach der Proteolyse-Chelationstheorie der Zahnkaries werden organische und anorganische Bestandteile des Zahnschmelzes mehr oder weniger gleichzeitig abgebaut. Die Ergebnisse dieser Untersuchungen zeigen, dass 1. mineralische Bestandteile des Schmelzes die mikrobiologische Zersetzung des Keratins fördern und dass 2. die enzymatische Zersetzung des Keratins Stoffe entwickelt, welche die Apatitauflösung mittels Chelation fördern.

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This investigation was supported in part by a research grant D-182(C) from the National Institute of Dental Research of the National Institutes of Health, United States Public Health Service.     

Pineal inhibition of compensatory testicular enlargement in light-deprived hamsters

Zusammenfassung Halbseitige Kastration fÜhrt bei geblendeten Goldhamstermännchen mit Atrophie der Geschlechtsorgane nur nach Pinealektomie zu kompensatorischer Hodenhypertrophie.

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Supported by grant No. HD-02937 United States Public Health Service.

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U.S.P.H.S. Career Development Awardee (No. 1 K04 HD42398).     

Monoamines in sympathetic ganglia studied with fluorescence microscopy

Zusammenfassung Sympathische Ganglien wurden mit einer spezifischen und besonders empfindlichen histochemischen Methode zum Nachweis gewisser Monoamine studiert. Die meisten Zellkörper der Ganglienzellen enthalten im Cytoplasma mehr oder weniger einer primären Monoamine, wahrscheinlich Noradrenalin. In prävertebralen Ganglien wurden auch adrenerge Nevenendigungen nachgewiesen, die synaptische Verbindungen mit den Ganglienzellen eingehen.

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This work has been supported by grants from the United States Public Health Service (NB 02854-03), the Foundation Therese and Johan Anderssons Minne, and Karolinska Institutet.     

Progesterone stimulates energy-dependent contraction of swollen heart mitochondria

Summary Progesterone stimulates the rate and efficiency of respiration-dependent contraction of heart mitochondria in nitrate salts at alkaline pH. Ion extrusion under these conditions is normally slow and inefficient due to the elevated permeability of the membrane to monovalent cations through a putative uniport pathway. Progesterone also inhibits passive swelling under these conditions and appears to act by restricting cation influx through the uniport pathway.This study was supported in part by United States Public Health Service grant No. HL09364.