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1.
萱草(Hemerocallis hybrida)再生植株过程中根的诱导   总被引:4,自引:0,他引:4  
利用1/2MS培养基作为基本培养基,附加不同浓度的NAA作为外源激素,分别对萱草愈伤组织块和无根再生进行根的发生诱导试验,结果:愈伤组织上均不易生根,而无根再生苗根的诱导效果较好,特别是在含有0.075mg/L NAA的培养基上,生根数量和生根率均最佳,为工厂化生产提供了相关的技术资料。  相似文献   

2.
欧林达夏橙组织培养研究   总被引:3,自引:0,他引:3  
比较不同基本培养基、不同外植体、不同生长调节物质种类及配比对欧林达夏橙实生苗芽诱导及根分化的影响.结果表明,子叶节段诱导芽效果较佳,MS为基本培养基附加生长调节物质BA2mg/L,NAA0.2mg/L适于芽的诱导,White为基本培养基附加生长调节物质IBA2mg/L对根的诱导较佳,生根率达100%,移栽成活率达94%.  相似文献   

3.
芦笋组织培养生根技术   总被引:2,自引:0,他引:2  
切取生长势一致的芦笋顶芽2~3mm,接种在添加不同浓度KT和IBA两种激素的生根培养基中,结果表明:当IBA浓度为1mg/L,KT浓度为0.02mg/L时,生根率可达到90%.另外,在相同条件下不同芦笋品种生根情况差异明显.添加NAA0.1mg/L或PP3331mg/L可促进难生根品种生根,改善生根情况.  相似文献   

4.
非洲紫罗兰的组织培养和植株再生   总被引:1,自引:0,他引:1  
对非洲紫罗兰的组织培养研究表明,外植体消毒以0.1%的升汞溶液浸泡7~8min为宜;不同激素浓度对其芽丛的形成、分化及根的形成有不同的影响;生长素与细胞分裂素的比例决定着芽丛的形成;小苗在1/2MS培养基上生根最好,6-BA对其生根有抑制作用.各培养阶段的最适培养基分别为,芽诱导培养基MS 2mg/L 6-BA 0.2mg/L NAA,芽分化培养基MS 1mg/L 6-BA 0.1mg/L NAA,壮苗培养基MS 0.1 mg/L NAA,生根培养基1/2MS 0.2mg/L IBA。  相似文献   

5.
闵炜  陈志萍 《江西科学》2009,27(2):219-223
在试管中诱导金边瑞香芽苗生根,要同时添加NAA和IBA或NAA和IAA才能形成良好的根系,添加足够的蔗糖对提高生根率,增加每株生根数和根的长度都至关重要。通过对6种生根培养基比较,确定1/2MS+NAA0.5mg/L+IBA0.5mg/L+3%蔗糖为最佳的诱导生根培养基,平均生根率达84.6%。  相似文献   

6.
以三尖杉茎段诱导的腋芽苗为外植体进行生根诱导研究.结果表明,改良WH附加一定浓度的IBA能诱导三尖杉试管苗生根,NAA单独处理时未能诱导三尖杉不定根的发生;IBA、NAA共同使用时生根诱导效果比IBA单独使用时好,其中以改良WH IBA3.0mg/L NAA1.0mg/L培养基组合生根诱导效果较好,生根率为60.7%.  相似文献   

7.
中国蕙兰(金蕙)试管苗生根培养研究   总被引:3,自引:0,他引:3  
用中国惹兰品种(金蕙)的茎尖诱导产生的原球茎(根状茎)在继代过程中形成的无根幼苗为材料,研究其在不同培养基、不同生长素、不同蔗糖浓度下的生根情况,结果表明:最适宜生根的培养基为1/2MS,30d生根率达100%,根质好,苗壮;生长索生根试验证明:NAA优于IBA,NAA的最佳使用浓度为0.2~0.3mg/L;在培养基中加入30g/L蔗糖(市售白砂糖),不仅生根快,生根率高,且根质好,苗壮均匀。  相似文献   

8.
生长素对乔纳金苹果试管苗生根的影响   总被引:1,自引:0,他引:1  
以三倍体苹果品种乔纳金试管苗顶部茎段为试材,将其接种于添加不同生长素及其组合的MS(基本培养基)中,研究IAA、IBA、NAA 3种生长素对苹果试管苗生根的不同效应。结果表明:3种生长素均可诱导乔纳金试管苗生根,且组合处理的效应普遍优于单种激素,其中以MS IBA 0.8mg/L IAA 0.7mg/L为最好,其生根率达92%。  相似文献   

9.
以猪笼草的幼嫩茎段为外植体进行离体组织培养及植株再生,研究结果表明:以液体培养基1/2MS+6-BA1.0mg/I.+NAA0.1mg/L对芽的初始诱导以及固体培养基1/2MS+6-BA2.0mg/L+NAA0.1mg/L对不定芽的增殖效果最好,增殖率达419%;生根培养基以1/4MS+IBA0.5mg/L+活性碳(0.01%)为最佳,生根率可达100%.  相似文献   

10.
湿地松的组织培养及植株再生   总被引:19,自引:4,他引:19  
在以湿地松实生无菌苗带子叶顶芽为外植体诱导丛生芽的过程中,激素的种类及浓度、处理时间等对丛生芽的诱导影响较大,表现为:单独使用6-BA即可诱导丛生芽分化,但NAA与6BA协同作用效果更佳;外植体在改良GD 5mg/L 6-BA 0.1mg/L NAA的培养基上培养4~5周,其丛生芽诱导率最高达95%。将丛生芽单个切下继代培养在改良GD 3.5mg/L 6-BA 0.05mg/L NAA的培养基上时增殖较快。改良GD培养基中添加0.05~0.1mg/L生长素(NAA)或0.5g/L活性炭(AC)能明显促进丛生芽伸长;培养基中的大量元素减半则不利于湿地松丛生芽的伸长生长。将伸长的丛生芽单个切下置于生根培养基中,4周后生根率达53.3%,移栽成活率达85%。  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

13.
王慧 《科技信息》2008,(10):240-240
Wuthering Heights, Emily Bronte's only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.  相似文献   

14.
The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.  相似文献   

15.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

16.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

17.
Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.  相似文献   

18.
正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-  相似文献   

19.
We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-  相似文献   

20.
正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that  相似文献   

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