不同胃疾病患者血清胃蛋白酶原亚群的变化

目的 探讨不同胃疾病患者胃黏膜病理改变过程中,血清胃蛋白酶原(PC)Ⅰ和Ⅱ的变化规律.方法 选择已行常规胃镜检查和病理活检确诊的245例患者.其中非萎缩性胃炎53例,慢性萎缩性胃炎20例,胃溃疡36例,十二指肠溃疡31例,进展期胃癌69例,早期胃癌13例,胃癌术后23例,并设正常对照组36例,用酶联免疫吸附实验(ELISA)测定其血清PGⅠ及PGⅡ,并计算PGⅠ/PGⅡ的比值(PGK).结果 与正常对照组比较,早期胃癌和进展期胃癌患者血清PG Ⅰ和PG Ⅰ/Ⅱ比值均显著降低(P<0.005),胃溃疡患者血清PGⅠ升高(P<0.05),十二指肠溃疡患者血清PG Ⅰ和PGⅡ升高,PGI/PGⅡ比值升高(P<0.05);进展期胃癌血清PG Ⅰ、早期与进展期胃癌PGR均显著低于萎缩性胃炎组(P<0.001).胃癌组中以PG Ⅰ≤70μg/L和PGR≤6作为阳性指标,同时符合这两项的确诊率为62.1%,特异性为94.2%.结论 血清PG Ⅰ、PG Ⅱ含量的变化及PGR值与胃黏膜病变有关,血清PG Ⅰ和PG Ⅰ/PGⅡ比值的降低,是胃癌发生的危险因素,可以作为人群筛查和辅助诊断胃癌的一项血清学指标.     

胃蛋白酶原亚群与胃部疾病相关性研究

为测定胃蛋白酶原亚群(pepsinogenI PGI,pepsinogenⅡPGⅡ)在健康体检者及胃部疾病患者的血清含量,探讨血清中PG亚群检测对萎缩性胃炎和胃癌早期诊断的临床意义,利用放射免疫法(radioimmunoassay RIA)检测35例健康体检者、18例胃溃疡、30例萎缩性胃炎和30例胃癌患者血清中PGⅠ、PGⅡ及PGⅠ/PGⅡ比值的变化,比较正常组与各疾病组的胃蛋白酶原含量。结果:(1)与正常组相比,胃溃疡组PGⅠ、PGⅡ升高,以PGⅡ增高明显,而PGⅠ/PGⅡ比值降低;萎缩性胃炎和胃癌组PGⅠ水平均呈下降趋势,而PGⅡ水平变化不明显,PGⅠ/PGⅡ比值下降。(2)与溃疡组相比,萎缩性胃炎和胃癌组PGⅠ、PGⅡ及PGⅠ/PGⅡ比值均明显降低。(3)与萎缩性胃炎组相比,胃癌组PGⅠ、PGⅡ及PGⅠ/PGⅡ比值虽略低于萎缩性胃炎,但差异无统计学意义。(4)当PGⅠ≤80 ng/mL和PGⅠ/PGⅡ≤6时,检出萎缩性胃炎的特异度和灵敏度分别为94.3%和53.3%。说明血清PGⅠ和PGⅠ/PGⅡ比值的降低,是萎缩性胃炎及胃癌人群筛查和辅助诊断的一项血清学指标。血清PGI≤80 ng/mL且PGⅠ/PGⅡ≤6时检测萎缩性胃炎具有较好的特异度和灵敏度。测定血清PG亚群的方法简单易行,适于初筛萎缩性胃炎及胃癌人群及胃镜检查禁忌者。     

胃溃疡性病变与血清胃蛋白酶原水平的关系

测定97例溃疡型胃癌(GC)和135例胃溃疡(GU)患者的血清PG水平,并与39例胃黏膜基本正常者(NOR)作对比分析.血清PGⅠ水平在GU和GC组明显低于NOR组(P均＜0.01),而GC组又明显低于GU组(P＜0.01);PGⅡ在GC组明显低于NOR组(P＜0.05);PGⅠ/PGⅡ值GC组明显低于NOR组和GU组(P均＜0.01).胃底胃体癌患者血清PGⅠ水平明显低于贲门癌患者(P＜0.05).提示血清PGⅠ水平和PGⅠ/PGⅡ值降低是溃疡型GC的危险因素,检测血清PG水平有助于临床对胃良恶性溃疡的鉴别诊断.     

PG与HP检测相关性的探讨

目的:分析HP感染与PG水平间存在的相关性.方法:对HP检测为阳性的标本采用ELISA法检测PG Ⅰ、PG Ⅱ和PG Ⅰ/PG Ⅱ比值;对于PG Ⅰ、PG Ⅱ和PG Ⅰ/PG Ⅱ比值异常的标本分别采用金标法检测HP抗体.结果:HP阳性组与对照组相比较,PG Ⅰ、PG Ⅱ和PG Ⅰ/PG Ⅱ比值均有显著差异(P＜0.01);PG Ⅰ、PG Ⅱ和PG Ⅰ/PG Ⅱ异常组与对照组相比较,HP抗体阳性率均无显著差异(P＞0.01).结论:我国是胃癌发病率较高的国家之一,因此建议检出HP阳性的病例均要进行PG检测,PG的优先检测对胃病或胃癌的防治和诊断有重要意义.     

影响胃癌根治术后复发转移的因素

目的 探讨影响胃癌根治术后复发转移的因素.方法 回顾性分析302例胃癌患者根治术后肿瘤复发转移的情况,并采用Logistic进行单因素和多因素回归分析.结果 本组患者根治术后复发转移率为45.4%,术后2年内复发转移率73.7%,2～5年复发转移率21.9%,5年后复发转移率4.4%;单一复发转移占83.2%, 多部位复发转移占16.8%.单因素分析显示胃癌根治术后复发转移与肿瘤大小、原发灶部位、癌细胞分化程度、大体分型、癌细胞授润深度、辅助化疗、术后病理、TNN分期有关,经过多因素回归分析后显示肿瘤大小、辅助化疗、TNM分期为胃癌根治术后复发转移的独立因素.结论 胃癌根治术后复发转移大部分在术后2年内,以单一复发转移为主;肿瘤大小、TNM分期及辅助化疗为胃癌根治术后复发转移的独立影响因素.     

胃癌患者基质蛋白酶-9血浆浓度的变化

通过对127例胃癌根治手术或姑息手术的患者纳入本研究,最终78例完成本实验,其中51例患者术后胃癌复发或发生转移癌,27例未发生胃癌复发或转移癌.观察病理确诊的胃癌患者术前、术后及术后6个月的MMP-9的变化.发现胃癌术后MMP-9水平均明显降低(P＜0.05);复发病例MMP-9水平则明显升高,与未复发病例相比有显著性差别(P＜0.05),达到术前水平.     

基于磁珠的胃蛋白酶原Ⅰ和Ⅱ双标记时间分辨荧光免疫分析试剂的研制

针对胃癌及胃病患者血清中胃蛋白酶原Ⅰ(pepsinogenⅠ,PGⅠ)和胃蛋白酶原Ⅱ(pepsinogenⅡ,PGⅡ),研制以磁珠为载体的基于镧系元素铕和钐双标记时间分辨荧光免疫分析技术的检测试剂,能够同时检测患者血清中的PGⅠ和PGⅡ.实验采用双抗体夹心一步法反应模式,铕和钐作为高灵敏的荧光示踪物分别标记抗PGⅠ及PGⅡ抗体,并利用磁珠作为抗原抗体反应的载体,建立双标记磁珠时间分辨荧光免疫分析试剂,并对试剂的各项指标进行评价.实验结果表明,该试剂检测PGⅠ和PGⅡ的分析灵敏度分别达到0.15 ng/m L和0.16 ng/m L,检测范围分别为0.15 500.00 ng/m L和0.16 50.00 ng/m L,准确度和精密性指标均达到临床检测要求,61份临床考核样本经本试剂和雅培化学发光试剂测定,结果一致性较高,无显著差异.实验阐明了磁珠作为固相载体结合经典的时间分辨荧光免疫分析技术在检测PGⅠ和PGⅡ方面的应用潜能,该研究将在多种胃病的诊断、监测及高通量血液筛查等领域发挥巨大的临床应用价值.     

影响胃癌根治术后复发转移的因素

目的探讨影响胃癌根治术后复发转移的因素。方法回顾性分析302例胃癌患者根治术后肿瘤复发转移的情况,并采用Logistlc进行单因素和多因素回归分析。结果本组患者根治术后复发转移率为45．4％,术后2年内复发转移率73．7％,2-5年复发转移率21．％,5年后复发转移率4．4％;单一复发转移占83．2％,多部位复发转移占16．8％。单因素分析显示胃癌根治术后复发转移与肿瘤大小、原发灶部位、癌细胞分化程度、大体分型、癌细胞浸润深度、辅助化疗、术后病理、TNM分期有关,经过多因素回归分析后显示肿瘤大小、辅助化疗、TNN分期为胃癌根治术后复发转移的独立因素。结论胃癌根治术后复发转移大部分在术后2年内,以单一复发转移为主;肿瘤大小、1NM分期及辅助化疗为胃癌根治术后复发转移的独立影响因素。     

保乳简化根治术治疗乳腺癌42例报告

目的 :探讨乳腺癌保乳手术的方法与疗效。方法 :42例Ⅰ -Ⅱ期原发性乳腺癌 ,施行了局部扩大切除并腋窝淋巴结清扫 ,重视术后早期化疗 ,有效地预防复发和转移。结果 :术后半年随访 ,患者本人对保留乳房的满意率为100 % ,十分满意者91 %。经2～50mo随访 ,平均16mo ,仅1例患侧局部复发。无远处转移和死亡病例。结论 :Ⅰ—Ⅱ期乳腺癌行保乳手术 ,近期疗效满意 ,远期效果有待随访观察。     

胃癌患者血清miR-101与miR-25的动态变化及其诊断价值

目的:探讨miR-101与miR-25在胃癌术前术后血清中的表达及其与病理因素间的关系,探讨其是否能作为胃癌早期诊断的新型生物标志物.方法:应用实时荧光定量(PCR)检测58例胃癌(其中31例配对胃癌术前术后),30例胃癌癌前病变,30例正常对照血清miR-101与miR-25的相对表达量,比较miR-101与miR-25在胃癌术前术后表达差异,及其与病理参数的关系,绘制研究对象工作特征曲线(ROC),评估血清miR-101与miR-25对胃癌的临床诊断价值.结果:胃癌血清miR-101表达水平显著性低于正常对照组(P0.01)及胃癌癌前病变组(P0.01),而胃癌血清miR-25(P0.01)表达水平显著升高.胃癌术后血清miR-101表达水平明显高于术前(P=0.007),而血清miR-25在胃癌术后显著性降低(P=0.011).ROC曲线分析显示血清miR-101与miR-25对胃癌诊断价值较高[miR-101:ROC曲线下面积(Area Under roc Curve,AUC)=0.857,敏感度=0.710,特异度=0.881;miR-25:AUC=0.821,敏感度=0.702,特异度=0.800],而血清miR-101与miR-25联合诊断胃癌的AUC为0.905,其敏感性和特异性分别为75.9%和86.7%.进一步分析发现胃癌血清miR-101低表达与幽门螺旋杆菌(helicobacter pylori,Hp)感染有关,miR-101表达水平在感染HP的胃癌血清中显著低于未感染HP的胃癌血清(P=0.012);胃癌血清miR-101低表达与胃癌淋巴结转移(P=0.033)和临床分期(P=0.015)显著相关;胃癌血清miR-25在高临床分期(P=0.018)和淋巴结转移(P0.001)表达水平升高.结论:血清miR-101与miR-25可能作为胃癌术前术后动态监测和胃癌诊断的新型潜在标志物.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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Brief Introduction to “Journal of Jilin University( Science Edition) ”

<正>"Journal of Jilin University(Science Edition)"is a comprehensive academic journal in the fields of science sponsored by Jilin University and administrated by the Ministry of Education of the Peoples Republic of China.The journal started publication in 1955.The original name at starting publication was"Journal of Natural Science of Northeast People University",which was changed into"Acta Scientiarum Naturalium Universitatis Jilinensis"in 1958 owing to the name change of the university.The present journal name has